克氏锥虫的新过氧化物酶基因克隆表达研究(英文)

目的为了探索克氏锥虫的谷胱甘肽依赖的过氧化物酶活性。方法使用基因克隆与表达鉴定的方法学对克氏锥虫谷胱甘肽依赖的过氧化物酶基因克隆和表达。结果一个全新的、18kDa的克氏锥虫单拷贝过氧化物酶基因被完全克隆到E.coli的表达载体pHrcHis上,并测序。蛋白序列分析表明与基因库谷胱甘肽依赖的过氧化物酶有高度的同源性。其表达蛋白被纯化分离,测定其酶活性显示了良好的活性,并能被亚油酸-氢过氧化物饱和。结论结果证实克氏锥虫仍然存在过氧化物酶介导的生物代谢。

农杆菌介导仙客来遗传转化体系的建立

利用根癌农杆菌介导法对仙客来叶片进行遗传转化,研究影响仙客来转化的若干因素,建立一套高效的遗传转化体系。结果表明:2 d的预培养,OD600值约为0.8的工程菌液浓度,侵染时间15 min,4 d的共培养,延迟筛选10 d有利于获得较高的转化效率。PCR检测表明,APX基因已整合到仙客来基因组中,共获得4株转基因植株,转化率为9.3%。

FT Ⅰ, a novel positive myeloid-lineage-specific transcription regulatory element within the mouse myeloperoxidase gene enhancer, En 1

FT Ⅰ (AAAAGGGGAAGCAGAG), a poly purine ele-ment within the myloid-lineage specific enhancer (En 1) of the mouse myeloperoxidase gene [1, 2] has been fur-ther characterised. 1, FT Ⅰ functions as a myeloid-lineage specific transcription regulatory element; 2, WEHI 3BD+ cells have higher binding activity to FT Ⅰ and express the proteins which could form the unique DNA-protein com-plex(es) of FT Ⅰ;. 3, The essential sequence for the specific DNA-protein interactions of FT Ⅰ is AAAAGGGGAAGC; 4, South-western analysis in conjunction with the compe-tition assay of the proteins binding to FT Ⅰ, has revealed a 28 kd protein in WEHI 3BD+ cells that displays the properties of the putative transcription factor which acts through FT Ⅰ. These new findings have demonstrated both the functional myeloid-lineage specificity and the novelty of FT Ⅰ.