Mercury （Hg） is one of the commonly encountered heavy metals, which is widespread in inshore sediments of China. In order to investigate the toxicity of Hg on marine invertebrates, we studied the effects of the diva...Mercury （Hg） is one of the commonly encountered heavy metals, which is widespread in inshore sediments of China. In order to investigate the toxicity of Hg on marine invertebrates, we studied the effects of the divalent mercuricion （Hg2＋） （at two final concentrations of 0.0025 and 0.0050mgL-1, prepared with HgC12） on metallothionein （MT） content, DNA integrity （DNA strand breaks） and catalase （CAT） in the gills and hepatopancreas, antioxidant enzyme activities of superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPx）, in the hemolymph, gills and hepatopancreas of the portunid crab Chao,bdisjaponica for an experiment period up to 15 d. The results indicated that MT was significantly induced after 3 d, with a positive correlation with Hg2＋ dose and time in the hepatopancreas and a negative correlation with Hg2＋ dose and time in the gills. While CAT in the hemolymph was not detected, it increased in the hepatopancreas during the entire experiment; SOD and GPx in the three tissues were stimulated after 12h, both attained peak value and then reduced during the experimental period. Meanwhile, DNA strand breaks were all induced significantly after 12 h. These results suggested the detoxification strategies against Hg2＋ in three tissues of C. japonica.展开更多
基金supported by the 111 Project (B0804)the Agricultural Science and Technology Achievement Transfer Project of TianJin City of China (07050201)
文摘Mercury （Hg） is one of the commonly encountered heavy metals, which is widespread in inshore sediments of China. In order to investigate the toxicity of Hg on marine invertebrates, we studied the effects of the divalent mercuricion （Hg2＋） （at two final concentrations of 0.0025 and 0.0050mgL-1, prepared with HgC12） on metallothionein （MT） content, DNA integrity （DNA strand breaks） and catalase （CAT） in the gills and hepatopancreas, antioxidant enzyme activities of superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPx）, in the hemolymph, gills and hepatopancreas of the portunid crab Chao,bdisjaponica for an experiment period up to 15 d. The results indicated that MT was significantly induced after 3 d, with a positive correlation with Hg2＋ dose and time in the hepatopancreas and a negative correlation with Hg2＋ dose and time in the gills. While CAT in the hemolymph was not detected, it increased in the hepatopancreas during the entire experiment; SOD and GPx in the three tissues were stimulated after 12h, both attained peak value and then reduced during the experimental period. Meanwhile, DNA strand breaks were all induced significantly after 12 h. These results suggested the detoxification strategies against Hg2＋ in three tissues of C. japonica.
