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3种淡水环境中低核酸含量细菌的滤过性 被引量:1
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作者 刘杰 宋宇昊 +1 位作者 赵志鹏 王莹莹 《微生物学通报》 CAS CSCD 北大核心 2016年第9期1895-1901,共7页
【目的】增加低核酸含量(LNA)细菌与过滤性细菌之间的认识。【方法】采用流式细胞技术(FCM)、变性梯度凝胶电泳(DGGE)及统计学分析研究3种典型淡水环境中细菌群落与滤过性。【结果】LNA细菌与过滤性细菌在数量上具有很好的相关性(y=0.64... 【目的】增加低核酸含量(LNA)细菌与过滤性细菌之间的认识。【方法】采用流式细胞技术(FCM)、变性梯度凝胶电泳(DGGE)及统计学分析研究3种典型淡水环境中细菌群落与滤过性。【结果】LNA细菌与过滤性细菌在数量上具有很好的相关性(y=0.646x+22.42,R2=0.984,P<0.01),细菌的滤过性不仅与细菌大小有关,还与细胞整体形状及其伸缩性有关;细菌群落组织与LNA细菌比重呈负相关性,而与HNA细菌呈正相关性。【结论】0.45μm膜过滤对水样微生物群落中的LNA细菌具有极强的筛选效果;细菌群落组织与其基于流式细胞技术测定的基因含量具有密切联系。 展开更多
关键词 流式细胞技术(FCM) 低核酸含量(LNA)细菌 高核酸含量(HNA)细菌 过滤性细菌 变性梯度凝胶电泳(DGGE) 群落组织
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Performance of biotrickling filters for hydrogen sulfide removal under starvation and shock loads conditions 被引量:1
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作者 Lan-he ZHANG Xiu-li MENG +1 位作者 Ying WANG Li-dan LIU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2009年第8期595-601,共7页
In the industrial operation of biotrickling filters for hydrogen sulfide(H2S) removal,shock loads or starvation was common due to process variations or equipment malfunctions.In this study,effects of starvation and sh... In the industrial operation of biotrickling filters for hydrogen sulfide(H2S) removal,shock loads or starvation was common due to process variations or equipment malfunctions.In this study,effects of starvation and shock loads on the performance of biotrickling filters for H2S removal were investigated.Four experiments were conducted to evaluate the changes of biomass and viable bacteria numbers in the biotrickling filters during a 24-d starvation.Compared to biomass,viable bacteria numbers decreased significantly during the starvation,especially when airflow was maintained in the absence of spray liquid.During the subsequent re-acclimation,all the bioreactors could resume high removal efficiencies within 4 d regardless of the previous starvation conditions.The results show that the re-acclimation time,in the case of biotrickling filters for H2S removal,is mainly controlled by viable H2S oxidizing bacteria numbers.On the other hand,the biotrickling filters can protect against shock loads in inlet fluctuating H2S concentration after resuming normal operation.When the biotrickling filters were supplied with H2S at an input of lower than 1700 mg/m3,their removal efficiencies were nearly 98% regardless of previous H2S input. 展开更多
关键词 Biotrickling filter STARVATION Shock loads ODOR Hydrogen sulfide
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