Two soy protein 11S fractions with different surface sulfhydryl contents were prepared.Utilizing analytical ultracentrifugation,the effects of storage time and hydrogen peroxide at different concentrations(0.5-100 mmo...Two soy protein 11S fractions with different surface sulfhydryl contents were prepared.Utilizing analytical ultracentrifugation,the effects of storage time and hydrogen peroxide at different concentrations(0.5-100 mmol/L)on the two 11S fractions were investigated.Results show that after removing 2-mercaptoethanol(2-ME)by size exclusion chromatography,the 11S fraction with high surface sulfhydryl content(2.0 mol sulfhydryl/mol 11S)progressively formed 15S and 21S in dilute solutions during storage at 4℃ for 82 days.While,the 11s fraction with low surface sulfhydryl content(0.2 mol sulfhydryl/mol 11S)was stable under the same condition.Moreover,after treating the 11s with high surface sulfhydryl content with 1 mmol/L H_(2)O_(2),the weight percentage of 15S reached the maximum value of 20%.The 15S induced by air and H_(2)O_(2)could be totally converted to 11S with the addition of 10 mmol/L 2-ME,which could be attributed to that the disulfide bond linking two 11S molecules is on the surface of the 15S and easily accessible to the reducing agent 2-ME.This study helps us to deeply understand the formation mechanism of 15S and the stability of 11S.展开更多
[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic h...[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.展开更多
基金supported by the National Natural Science Foundation of China(No.22173092 and No.21674107).
文摘Two soy protein 11S fractions with different surface sulfhydryl contents were prepared.Utilizing analytical ultracentrifugation,the effects of storage time and hydrogen peroxide at different concentrations(0.5-100 mmol/L)on the two 11S fractions were investigated.Results show that after removing 2-mercaptoethanol(2-ME)by size exclusion chromatography,the 11S fraction with high surface sulfhydryl content(2.0 mol sulfhydryl/mol 11S)progressively formed 15S and 21S in dilute solutions during storage at 4℃ for 82 days.While,the 11s fraction with low surface sulfhydryl content(0.2 mol sulfhydryl/mol 11S)was stable under the same condition.Moreover,after treating the 11s with high surface sulfhydryl content with 1 mmol/L H_(2)O_(2),the weight percentage of 15S reached the maximum value of 20%.The 15S induced by air and H_(2)O_(2)could be totally converted to 11S with the addition of 10 mmol/L 2-ME,which could be attributed to that the disulfide bond linking two 11S molecules is on the surface of the 15S and easily accessible to the reducing agent 2-ME.This study helps us to deeply understand the formation mechanism of 15S and the stability of 11S.
文摘[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.
