Against the deficiencies of component-based software(CBS) reliability modeling and analysis,for instance,importing too many assumptions,paying less attention to debugging process without considering imperfect debuggin...Against the deficiencies of component-based software(CBS) reliability modeling and analysis,for instance,importing too many assumptions,paying less attention to debugging process without considering imperfect debugging and change-point(CP) problems adequately,an approach of CBS reliability process analysis is proposed which incorporates the imperfect debugging and CP.First,perfect/imperfect debugging and CP are reviewed.Based on the queuing theory,a multi-queue multichannel and infinite server queuing model(MMISQM) is presented to sketch the integration test process of CBS.Meanwhile,considering the effects of imperfect debugging and CP,expressions for fault detection and correction are derived based on MMISQM.Numerical results demonstrate that the proposed model can sketch the integration test process of CBS with preferable performance which outperforms other models.展开更多
Two Lecanicillium lecanii isolates, ATCC26854 and V3, were evaluated for spore production in liquid media. Both isolates have interesting known properties for the production of high concentrations of chitinases （ATCC...Two Lecanicillium lecanii isolates, ATCC26854 and V3, were evaluated for spore production in liquid media. Both isolates have interesting known properties for the production of high concentrations of chitinases （ATCC26854）, and an outstanding pathogenic activity against the green cabbage aphid, Brevicorine brassiacae （L.）. The isolates were grown on thirteen different liquid media, which had been used to produce other entomopathogenic Hyphomycetes. Experiments were carried out at 27 -4- 1 ℃ with a 12：12 photoperiod using shake flasks. The production of spores was quantified during a seven days period, and the effects of the media were evaluated by determining spore concentration and morphology. Submerged conidia yields were higher with ATCC26854 than with V3 in all thirteen media, while higher concentrations （5.3×10^9, 4.6 ×10^9 and 3.4 ×10^9 conidia/mL） were found with ATCC26854 isolate in the Camaron, Minerales and Jenkins-Prior medium, respectively; lower yields （2.3 ×10^8, 2.2 ×10^8 and 2.3 ×10^8 conidia/mL） were found with the V3 isolate in Catroux, TKI and Camaron media, respectively. Spore production curves were adjusted to different sigmoid models. The process was better explained by the Richards model （r^2 = 0.99）. Concerning conidia morphology, submerged conidia seemed to look like aerial conidia, but they were different in size （ATCC26854 2.73-6.99 μm and V3 5.28-14.29 μm）; however, the dimensions fall within the ranges reported for L. lecanii. The analysis of shake flask cultures with the Richards model allowed selecting two low-cost liquid medium, Camaron and Jenkins-Prior, for scaling up conidia production for use in aphid biological control programs.展开更多
基金Supported by the National High Technology Research and Development Program of China(No.2008AA01A201)the National Natural ScienceFoundation of China(No.60503015)+1 种基金the National Key R&D Program of China(No.2013BA17F02)the Shandong Province Science andTechnology Program of China(No.2011GGX10108,2010GGX10104)
文摘Against the deficiencies of component-based software(CBS) reliability modeling and analysis,for instance,importing too many assumptions,paying less attention to debugging process without considering imperfect debugging and change-point(CP) problems adequately,an approach of CBS reliability process analysis is proposed which incorporates the imperfect debugging and CP.First,perfect/imperfect debugging and CP are reviewed.Based on the queuing theory,a multi-queue multichannel and infinite server queuing model(MMISQM) is presented to sketch the integration test process of CBS.Meanwhile,considering the effects of imperfect debugging and CP,expressions for fault detection and correction are derived based on MMISQM.Numerical results demonstrate that the proposed model can sketch the integration test process of CBS with preferable performance which outperforms other models.
文摘Two Lecanicillium lecanii isolates, ATCC26854 and V3, were evaluated for spore production in liquid media. Both isolates have interesting known properties for the production of high concentrations of chitinases （ATCC26854）, and an outstanding pathogenic activity against the green cabbage aphid, Brevicorine brassiacae （L.）. The isolates were grown on thirteen different liquid media, which had been used to produce other entomopathogenic Hyphomycetes. Experiments were carried out at 27 -4- 1 ℃ with a 12：12 photoperiod using shake flasks. The production of spores was quantified during a seven days period, and the effects of the media were evaluated by determining spore concentration and morphology. Submerged conidia yields were higher with ATCC26854 than with V3 in all thirteen media, while higher concentrations （5.3×10^9, 4.6 ×10^9 and 3.4 ×10^9 conidia/mL） were found with ATCC26854 isolate in the Camaron, Minerales and Jenkins-Prior medium, respectively; lower yields （2.3 ×10^8, 2.2 ×10^8 and 2.3 ×10^8 conidia/mL） were found with the V3 isolate in Catroux, TKI and Camaron media, respectively. Spore production curves were adjusted to different sigmoid models. The process was better explained by the Richards model （r^2 = 0.99）. Concerning conidia morphology, submerged conidia seemed to look like aerial conidia, but they were different in size （ATCC26854 2.73-6.99 μm and V3 5.28-14.29 μm）; however, the dimensions fall within the ranges reported for L. lecanii. The analysis of shake flask cultures with the Richards model allowed selecting two low-cost liquid medium, Camaron and Jenkins-Prior, for scaling up conidia production for use in aphid biological control programs.
