OBJECTIVE To detect the differences in gene expression of nonhomologous end-joining pathway including Ku70, Ku80, ERCC4, lig4 and DNA-PKcs between human primary gliomas and normal brain tissues, and furthermore, to ex...OBJECTIVE To detect the differences in gene expression of nonhomologous end-joining pathway including Ku70, Ku80, ERCC4, lig4 and DNA-PKcs between human primary gliomas and normal brain tissues, and furthermore, to explore the underlying mechanism for the expression alteration.METHODS The expression levels of Ku70, Ku80, ERCC4, lig4 and DNA-PKcs in 36 specimens of glioma and 12 specimens of normal brain tissue were measured using SYBR green-based real- time quantitative PCR. Methylation of DNA-PKcs was detected through methylation-specific PCR （MSP）.RESULTS There was no significant difference in expression of Ku70, Ku80, ERCC4 and lig4 between human primary gliomas and normal brain tissues （P 〈 0.05）, while DNA-PKcs were significantly up-regulated （P = 0.002）. The expression of DNA- PKcs was significantly higher in patients with grade III and IV diseases compared to patients with grade II disease or in normal brain tissues （P 〈 0.05）. Moreover, glioma tissue showed weaker methvlation than normal brain tissue.CONCLUSION The up-regulation of the DNA-PKcs may be associated with pathogenesis of glioma. Demethylation of DNA- PKcs promoter is an important reason for its up-regulation.展开更多
In this paper, we conduct research on the high-strength coating preparation methodology based on acid catalytic and Sol-Gel method. This method has been widely used in the preparation of various functional thin film, ...In this paper, we conduct research on the high-strength coating preparation methodology based on acid catalytic and Sol-Gel method. This method has been widely used in the preparation of various functional thin film, film and protective film structure, etc. As a result of sol gel process continuously broaden the application field, this method has been more and more get the favor of people. Compared with other traditional preparation methods of inorganic material, sol-gel process has many characteristics. To adjust the solution acidity and add a small amount of acid or alkali can have the effect of＂ catalyst, its reaction process on sol to get and gel structure may also be affected. Our research analyze the topic theoretically and numerically which is meaningful.展开更多
The work describes the properties of soluble organic silicates and their applications to obtain nanocomposite materials. We analyzed the properties of the water-soluble high-modulus silicate systems and their technolo...The work describes the properties of soluble organic silicates and their applications to obtain nanocomposite materials. We analyzed the properties of the water-soluble high-modulus silicate systems and their technology for producing. The aim of this paper is the comparison properties of binders based on liquid glass containing strong organic bases silicates. We have shown how these systems are transformed from lower to higher oligomers through the formation of the silica sol and the implementation of the sol-gel process for these oligomers. We have conducted advanced research of various aspects of the use of these materials as the binder. Advantages of strong organic bases silicates in the preparation of heat resistant, nanocomposite materials are shown. Ways to obtaining quaternary ammonium silicates and their use to produce nanocomposites are proposed. Products obtained in this way can be used as a binder in the preparation of nanostruetured composite materials, water-based paints, coatings, etc. Modifiers have been proposed for making of hybrid nanostructured composite materials by a sol-gel process. There have been shown of structuring phenomena some aspects, synthesis and application of hybrid materials based on silica with grafted polymers. It has been shown, the possibility of modifying compositions using the nanostructuring agents such as tetrafurfuryloxysilane. This paper also describes methods for the synthesis of products for modifying a sol-gel process using organic soluble silicates. We are displaying their use for the production of new nanocomposite materials and coatings for protection against various external factors.展开更多
文摘OBJECTIVE To detect the differences in gene expression of nonhomologous end-joining pathway including Ku70, Ku80, ERCC4, lig4 and DNA-PKcs between human primary gliomas and normal brain tissues, and furthermore, to explore the underlying mechanism for the expression alteration.METHODS The expression levels of Ku70, Ku80, ERCC4, lig4 and DNA-PKcs in 36 specimens of glioma and 12 specimens of normal brain tissue were measured using SYBR green-based real- time quantitative PCR. Methylation of DNA-PKcs was detected through methylation-specific PCR （MSP）.RESULTS There was no significant difference in expression of Ku70, Ku80, ERCC4 and lig4 between human primary gliomas and normal brain tissues （P 〈 0.05）, while DNA-PKcs were significantly up-regulated （P = 0.002）. The expression of DNA- PKcs was significantly higher in patients with grade III and IV diseases compared to patients with grade II disease or in normal brain tissues （P 〈 0.05）. Moreover, glioma tissue showed weaker methvlation than normal brain tissue.CONCLUSION The up-regulation of the DNA-PKcs may be associated with pathogenesis of glioma. Demethylation of DNA- PKcs promoter is an important reason for its up-regulation.
文摘In this paper, we conduct research on the high-strength coating preparation methodology based on acid catalytic and Sol-Gel method. This method has been widely used in the preparation of various functional thin film, film and protective film structure, etc. As a result of sol gel process continuously broaden the application field, this method has been more and more get the favor of people. Compared with other traditional preparation methods of inorganic material, sol-gel process has many characteristics. To adjust the solution acidity and add a small amount of acid or alkali can have the effect of＂ catalyst, its reaction process on sol to get and gel structure may also be affected. Our research analyze the topic theoretically and numerically which is meaningful.
文摘The work describes the properties of soluble organic silicates and their applications to obtain nanocomposite materials. We analyzed the properties of the water-soluble high-modulus silicate systems and their technology for producing. The aim of this paper is the comparison properties of binders based on liquid glass containing strong organic bases silicates. We have shown how these systems are transformed from lower to higher oligomers through the formation of the silica sol and the implementation of the sol-gel process for these oligomers. We have conducted advanced research of various aspects of the use of these materials as the binder. Advantages of strong organic bases silicates in the preparation of heat resistant, nanocomposite materials are shown. Ways to obtaining quaternary ammonium silicates and their use to produce nanocomposites are proposed. Products obtained in this way can be used as a binder in the preparation of nanostruetured composite materials, water-based paints, coatings, etc. Modifiers have been proposed for making of hybrid nanostructured composite materials by a sol-gel process. There have been shown of structuring phenomena some aspects, synthesis and application of hybrid materials based on silica with grafted polymers. It has been shown, the possibility of modifying compositions using the nanostructuring agents such as tetrafurfuryloxysilane. This paper also describes methods for the synthesis of products for modifying a sol-gel process using organic soluble silicates. We are displaying their use for the production of new nanocomposite materials and coatings for protection against various external factors.
