[Objective] The aim was to explore the relationship of pollen types and blooming plants,pollen fertility and meteorological factors during the falling process of pollen in the atmosphere.[Method] The falling record of...[Objective] The aim was to explore the relationship of pollen types and blooming plants,pollen fertility and meteorological factors during the falling process of pollen in the atmosphere.[Method] The falling record of pollen in Lanzhou University in spring in 2009 was obtained by 10 m platform natural falling method.The pollen falling number,duration,relationship among falling types and surrounding plants,as well as weather were discussed.[Result] Main pollen types included Populus L.,Salix,Paulownia and Platanaceae.Its pollen falling number,duration was consistent with the flowering period of corresponding plants in the garden;the classification and richness of falling of pollen was influenced by wind.The larger the wind was,the richer the variety become.There was basically no pollen from outside.The authors failed to observe the distinct changes of palynological assemblage in sandy storm weather.[Conclusion] The study could lay basis for the study of the modern progress of natural plants pollen.展开更多
Based on field investigation and study of petrology, mineralogy, geochemistry and isotopic geochronology for the granodiorite, the authors discussed the formation setting of the granodiorite. The pluton is calci alkal...Based on field investigation and study of petrology, mineralogy, geochemistry and isotopic geochronology for the granodiorite, the authors discussed the formation setting of the granodiorite. The pluton is calci alkalic and peraluminous granite in which light rare earth elements (LREE) are intensively enriched, heavy rare earth elements are relatively poor, the ratio of LREE to heavy rare earth elements (HREE) is 7.12, and the fractionation of REE is good. The pluton formed in late orogenic setting which is relatively steady.展开更多
AIM: To investigate whether birch pollen allergy symptoms are linked with gut microbiota changes and whether probiotics have an effect on these. METHODS: Forty seven children with confirmed birch pollen allergy were...AIM: To investigate whether birch pollen allergy symptoms are linked with gut microbiota changes and whether probiotics have an effect on these. METHODS: Forty seven children with confirmed birch pollen allergy were randomized to receive either a probiotic combination of Lactobacillus acidophilus (L. acidophilus) NCFM^TM (ATCC 700396) and Bifidobacterium lactis (B. lactis) BI-04 (ATCC SD5219) or placebo in a double-blind manner for 4 mo, starting prior to onset of the birch pollen season. Symptoms were recorded in a diary. Blood samples were taken for analysis of cytokines and eosinophils. Fecal samples were analysed for microbiota components, calprotectin and IgA. Nasal swabs were taken for analysis of eosinophils.RESULTS: The pollen season induced a reduction in Bifldobacterium , Clostridium and Bacteroides which could not be prevented by the probiotic intervention. During the intervention, significantly higher numbers ofB. lactis 11.2 × 10^7 ± 4.2 ×10^7 vs 0.1 × 10^7 ± 0.1 × 10^7 bacteria/g feces (P 〈 0.0001) and L. acidophilus NCFMTM 3.5 × 10^6 ± 1.3 × 10^6 vs 0.2 × 10^6 ±0.1 × 10^6 bacteria/g feces (P 〈 0.0001) were observed in the probiotic group compared to the placebo group.During May, there was a tendency for fewer subjects, (76.2% vs 95.2%, P = 0.078) to report runny nose, while during June, fewer subjects, 11.1% vs 33.3%, reported nasal blocking in the probiotics group (P = 0.101). Concomitantly, fewer subjects in the probiotic group had infiltration of eosinophils in the nasal mucosa compared to the placebo group, 57.1% vs 95% (P = 0.013). Eye symptoms tended to be slightly more frequent in the probiotic group, 12.5 d [interquartile range (IQR) 6-18] vs 7.5 d (IQR 0-11.5) (P = 0.066) during May. Fecal IgA was increased in the placebo group during the pollen season; this increase was prevented by the probiotics (P = 0.028). CONCLUSION: Birch pollen allergy was shown to be associated with changes in fecal microbiota composition. The specific combination of probiotics used was shown to prevent the pollen-induced infiltration of eosinophils into the nasal mucosa, and indicated a trend for reduced nasal symptoms.展开更多
The growing trend of network virtualization results in a widespread adoption of virtual switches in virtualized environments. However, virtual switching is confronted with great performance challenges regarding packet...The growing trend of network virtualization results in a widespread adoption of virtual switches in virtualized environments. However, virtual switching is confronted with great performance challenges regarding packet classification especially in Open Flow-based software defined networks. This paper first takes an insight into packet classification in virtual Open Flow switching, and points out that its performance bottleneck is dominated by flow table traversals of multiple failed mask probing for each arrived packet. Then we are motivated to propose an efficient packet classification algorithm based on counting bloom filters. In particular, counting bloom filters are applied to predict the failures of flow table lookups with great possibilities, and bypass flow table traversals for failed mask probing. Finally, our proposed packet classification algorithm is evaluated with real network traffic traces by experiments. The experimental results indicate that our proposed algorithm outperforms the classical one in Open v Switch in terms of average search length, and contributes to promote virtual Open Flow switching performance.展开更多
Four concentrations of CCC were used to treat pepper seedlings.The results indicated that 50-150mg·L-1 CCC decreased the content of malonaldehydic acid (MDA) and increased the content of soluble protein,ascorbic ...Four concentrations of CCC were used to treat pepper seedlings.The results indicated that 50-150mg·L-1 CCC decreased the content of malonaldehydic acid (MDA) and increased the content of soluble protein,ascorbic acid (AsA) and glutathion(GSH),and activity of superoxide dismutase (SOD) and catalase (CAT),whereas 200mg·L-1 CCC caused the soluble protein content and the CAT activity of Xiangyan 1 and the AsA content of Xiangyan 10 to descend.The comprehensive evaluation manifested that 100-150mg·L-1 CCC had obvious effects on water-logging resistance of pepper.Different varieties required different concentrations,eg,l00mg·L-1 CCC suited Xiangyan 1,while 150 mg·L-1 CCC suited Xiangyan 10.展开更多
Most research in the past using genetically modified crops (GM crops) has focused on the ecological safety of foreign gene (i.e., the gene flow), gene products (for example, Bt (Bacillus thuringiensis) protein), and t...Most research in the past using genetically modified crops (GM crops) has focused on the ecological safety of foreign gene (i.e., the gene flow), gene products (for example, Bt (Bacillus thuringiensis) protein), and the safety of transgenic food for humans. In this study, changes in both the species and amounts of low-molecular-weight components in cotton (Gossypium hirsutum L.) root exudates after foreign Bt gene overexpression were investigated under different nutritional conditions. Transgenic cotton containing Bt (Bt-cotton), supplemented with all the mineral nutrients, secreted more organic acids than the wild-type cotton (WT). When nitrogen was removed from the full-nutrient solution, the amount of organic acids secretion of Bt-cotton was lesser than that of WT. The roots of the transgenic cotton secreted lesser amounts of amino acids and soluble sugars than the WT roots in the full-nutrient solution. Deficiencies of P and K caused a large increase in the total amino acid and soluble sugar secretions of both Bt-cotton and WT, with larger increases observed in Bt-cotton. Because transferring the foreign Bt gene into cotton can result in alterations in the components of the root exudates, with the effect varying depending on the nutritional status, the cultivation of genetically modified crops, such as Bt-cotton, in soil environments should be more carefully assessed, and the possible effects as a result of the alterations in the root exudate components should be considered.展开更多
It is important to understand the pathogenesis of asthma induced by natural allergens, which could exclude the interference of artificial adjuvant and provide insights of natural immune response in the disease. In the...It is important to understand the pathogenesis of asthma induced by natural allergens, which could exclude the interference of artificial adjuvant and provide insights of natural immune response in the disease. In the present study, we show that Trichosanthin (TCS) could induce airway inflammation even without the help of alum. Furthermore, TCS appeared capable of replacing alum to promote OVA-specific airway inflammation. TCS induced accumulation of IL-4-producing eosinophils in peritoneum at an early stage and the adjuvant function of TCS was eliminated by blockage of IL-4 at this stage. Finally, the eosinophils triggered by TCS from WT mice, but not from IL-4- deficient mice were shown to function as adjuvant for the induction of OVA-specific Th2 responses. Our data indicate that TCS is not only an allergen, but also a Th2-typc adjuvant modulating the switching of immune responses to a Th2 pathway. This chain of events results from IL-4 production by eosinophils at an early stage of TCS-priming. In conclusion, TCS may be useful as a Th2 adjuvant, and innate immune cells, such as eosinophils, may be a good target to study the initiation of Th2 response.展开更多
During three years lasting studies on the territory of the city of Novi Sad, terrain studies of Ambrosia artemisiifolia L. distribution as well as mapping were performed on regulated and disordered green areas and on ...During three years lasting studies on the territory of the city of Novi Sad, terrain studies of Ambrosia artemisiifolia L. distribution as well as mapping were performed on regulated and disordered green areas and on arable areas. For mapping of ragweed distribution, partially modified method of Braun-Blanquet was used, and data processing was perfomed by specially designed program Ambrosia Spot Marker. Monitoring of this species was also done because of the occurrence of retrovegetation after mowing. During vegetation period, this allergenic weed species was controlled by multiple mowing, and rough ruderal sites outside settlements were treated by glyphosate at a rate of 1.2 kg ha^-1 -2.4 kg ha^-1. The study comprehended also monitoring of pollen concentrations in the air by Rotorod pollen sampler spore collector. A. artemisiifolia L. was established in 21 city zones on over 200 localities, on the banks of the river Danube, in Petrovaradin and Sremski Karlovci. Recommended mechanical measures such as mowing, drilling in the phase of germination, and chemical control measures, resulted in significant reduction ofA. artemisiifolia L. In the second half of the August 2009 the highest number of pollen grains was found in the amount of 783 pollen grains per m3 of air.展开更多
Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent fo...Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent for extraction process. Besides functioning as dyes, anthocyanins have a role as a good antioxidant, so it can be used to protect the fat content of fermented milk such as yoghurt. This experiment was to find out the effect of different solvent type on extraction pigment (aquadest: sitrat acid/lactic acid/sulfate acid/chloride acid) and to determine the effect of the addition of anthocyanins in protecting the fat content of yogurt on several levels addition. The research result showed that anthocyanin's pigment of red rose from petal flower's could be effective solvent extracted using lactic acid 0.02 M and increased fat stability of yoghurt. The anthocyanin pigment showed with HPLC analysis that pigment resulted TLC analysis at rite time 13.10 (as Cyanidin glucoside) and 22.61 (as Malvidin glucoside). Yoghurt that given anthocyanin pigment has proven that fat content can be maintained its 86.7% (fat content with 2% pigment as such as 0.117% and without pigment 0.087%) after being stored for 6 days at cold and room temperature.展开更多
We examined IgE levels in 4,312 samples of umbilical blood taken from children born into families with a positive history of allergy in one or both parents from 1998-2015. At the age often days, those with high IgE we...We examined IgE levels in 4,312 samples of umbilical blood taken from children born into families with a positive history of allergy in one or both parents from 1998-2015. At the age often days, those with high IgE were given Colinfant Newborn (a lyophilized non-pathogenic strain of E. coli) for one month, three times weekly. At 15 months and three years we investigated the levels of IgE, IgA and IgG, and the incidence of illness and allergy. The results revealed that allergy and high umbilical IgE is strongly linked with family history (p 〈 0.001). We also detected differences in seasonality, especially with regards to pollen allergies. About 80% of children treated with Colinfant had significantly reduced IgE and morbidity at 13-15 months and 3 years, and furthermore without any clinical signs of allergy. Surprisingly 20% of treated children did not have reduced IgE, and yet they did not have any clinical signs of allergy. Normalization of IgA and IgG was seen in 90% of treated subjects (p 〈 0.001). These levels significantly are correlated with an almost negligible morbidity up to 4 years of life. Our study strongly suggests a positive effect of physiological E. coli on the microbiome of children as evidenced by a significantly reduced incidence of allergy and morbidity when applied early in life.展开更多
The objectives of this study are to research the relationship between pectin and the softening of peach by soaking in citric acid solutions for 24 h at 35 ℃, pressurizing for 30 rain at 500 MPa or heating for 10 min....The objectives of this study are to research the relationship between pectin and the softening of peach by soaking in citric acid solutions for 24 h at 35 ℃, pressurizing for 30 rain at 500 MPa or heating for 10 min. Also, comparing high-pressure-induced jam (HP-jam) and heat-induced jam (H-jam) were evaluated. It was found that firmness of the peach decreased greatly when soaked at pH 2.0 〉 heated 〉 soaked at pH 2.2 or 2.5 〉 pressurized, respectively. About 88% of the peach pectin was water-soluble-pectin and high-methoxyl pectin, while low-methoxyl pectin was slight. During pressurization, the pectin did not change. However, pectin degraded through hydrolysis during heating; consequently, the middle lamella separated. Also, eight kinds of peach jam (65% sugar, pH 2.0 or pH 2.2, and 50% or 60% sugar, pH 2.5) were compared. Both color and flavor of HP-jam were better than H-jam. As the pH values were lower, L-, a-, b-values of jam became higher, and the jam became pinker. Raw peach contained about 0.3%-0.4% pectin, therefore, an addition of 0.6% pectin was needed for both HP- and H-jams. However, there was no great difference in rheology or sensory evaluation between HP- and H-jams.展开更多
The industrial chemistry of oils and fats is a mature technology, with decades of experience and refinement behind current practices, therefore some physico-chemical characteristics of eleven vegetable oils sunflower ...The industrial chemistry of oils and fats is a mature technology, with decades of experience and refinement behind current practices, therefore some physico-chemical characteristics of eleven vegetable oils sunflower oil (four different manufacturing companies), olive oil (three different manufacturing companies), corn oil, castor oil, coconut oil and canola oil in Iraqi Kurdistan region (Hawler city center) have been evaluated according to association of official analytical chemists (AOAC) (1995) official methods. The refractive index, viscosity, acid value, peroxide value, pH values, and values of specific gravity of the vegetable oil samples were measured at various different conditions. The results showed that some of the oil samples have unacceptable values in comparison with the standards.展开更多
The aim of this study was to determine the characteristics of the distribution of energy plant moisture content along the height of their shoots and the dynamics of moisture during storage in natural conditions. The s...The aim of this study was to determine the characteristics of the distribution of energy plant moisture content along the height of their shoots and the dynamics of moisture during storage in natural conditions. The shoots of Spartina, Miscanthus and willow were used in the study. Entire shoots were cut into sections of 10 cm and for each set in monthly cycles for six months moisture content was evaluated. After a month's storage of freshly cut shoots the biggest decrease of content moisture in the shoots of Spartina and Miscanthus was recorded, by 31% and 22%, respectively, and the lowest in willow shoots (12%). After sixth months of shoots storage the lowest moisture content (10%-12%) was reached in miscanthus. The most uneven moisture content along the height of shoots Spartina was characterized because on one third of the height from the bottom, the moisture content of shoots was 20%, and the top had moisture content in the range 5%-10%. Willow shoots were characterized by the smallest drop in moisture, and the final moisture content was about 23%, with the top part of moisture content of 10%-20%. The dynamics of moisture change during the six months of storage of grass shoots (Miscanthus and Spartina) in natural conditions under roofing was described by one power function regression, and willow by another one. Empirical models can be used to predict changes in moisture content of these plants in experiment conditions, since the coefficients of determination were 94.66% and 89.18%, respectively.展开更多
This work aims to study the development of the pericarp of the fruit of Elaeis guineensis Jacq. var. dura. The thickness, the water and the oil contents of its tissues are evaluated every two weeks, from pollination t...This work aims to study the development of the pericarp of the fruit of Elaeis guineensis Jacq. var. dura. The thickness, the water and the oil contents of its tissues are evaluated every two weeks, from pollination to the maturity of the fruit. The development of the oil palm fruit takes 5.5 months. The endocarp reaches its maximum thickness at the 70th DPP (day post-pollination), with a water content of 72%. It then starts its dehydration, while sclerifying. It therefore isolates the seed at start and later protects it. The mesocarp is visible at anthesis and its water content is close to 92%. From the 100th DPP, it begins a continuous dehydration associated, from the 130th DPP, with an active lipids biosynthesis. Ultimately, the pericarp of the oil palm fruit fulfills both functions, namely to protect the seed by early sclerification of the endocarp and ensure the dissemination of the species by the high oil content of the mesocarp. A comparative anatomy of the pericarp tissues of the three genotypes.of E. guineensis Jacq., during the first three weeks of fruit development, will enhance the understanding of the primary effect of sh gene.展开更多
[Objective] The aim was to provide certain theoretical basis for the protection of broccoli. [Method] Exogenous sodium nitroprusside was used to in the experiment. Under the condition of 20 ℃ and RH 95%, the optimal ...[Objective] The aim was to provide certain theoretical basis for the protection of broccoli. [Method] Exogenous sodium nitroprusside was used to in the experiment. Under the condition of 20 ℃ and RH 95%, the optimal SNP concentration of broccoli was determined. [Result] The exogenous SNP could effectively maintain the quality of broccoli, inhabited the yellowing of broccoli and the increasing of cell membrane permeability, and maintain the soluble solids content, decreased the degradation rate of chlorophyll and delayed the loss of VC. [Conclusion] Exogenous sodium nitroprusside can effectively maintain the quality of broccoli to keep it fresh.展开更多
The Baoshan Block is tectonically located in the middle segment of the Sibumasu plate. Granitic magmatism within the Baoshan Block has been considered weakly active due mainly to very limited exposures during the Hima...The Baoshan Block is tectonically located in the middle segment of the Sibumasu plate. Granitic magmatism within the Baoshan Block has been considered weakly active due mainly to very limited exposures during the Himalaya orogenic episode. The geochronological study on the buried Shuangmaidi granite has confirmed the existence of the Cenozoic granitoids in the Baoshan Block. The present study indicates that: (1) It is medium- to coarse-grained two mica phyric granite, characterized by high SiO2 (73.55%-77.16%) and low CaO (0.34%-1.38%) contents, with a total alkalis (K2O+Na2O) of 5.22%-8.03%, K2O/Na2O ratios of 0.24-1.79, and total rare earth elements (ZREE) of the granite between 85 and 125 ppb. All samples are enriched in light REE and exhibit medium negative Eu anomalies; and they show pronounced negative anomalies in Ba, Sr, Ti, and Nb but significant positive anomalies in K, Rb, U, Th, and Pb on mantle-normalized trace element patterns, indicating typi-cally peraluminous to strongly peraluminous S-type granite. (2) The zircon SHRIMP U-Pb ages of the granite are 36.27±0.48 Ma for the samples from ZK7-1 and 35.78±0.49 Ma for those from ZK0-1, respectively. The similar zircon ages from these two drill cores may suggest that the granite samples come from the same buried pluton. (3) 206pb/204pb values of the granite vary from 20.115 to 25.359, 207pb/204pb from 15.776 to 16.160, and 208pb/204pb from 39.236 to 41.285, showing the characteristics of radio- active lead anomaly of the upper crust. The (87Sr/86Sr)i values calculated on the average age of the two-mica orthoclase granite (36 Ma) range from 0.72524 to 0.77503 and eNd(t) values vary from -10.9 to -11.7. These data, along with the depleted-mantle Nd modal ages of 1.73-1.80 Ga, imply that the granites might have formed from partial melting of the Precambrian crystal basements. (4) On the Hf-Rb-Ta diagram, almost all the samples fall within the field of post-collision tectonic setting. The CaO/Na2O and A1203/TiO2 ratios suggest that the granitic magma may have formed from partial melting of clay-rich crustal materials with a pos- sible melting temperature of about 900℃ and a possible crystallization temperature of 775-795~C. (5) During the post-collision of the Himalaya orogen, with the southeastward extrusion of the Indochina continent resulting from the continuous northward indentation of the India continent into the Asia, the Gaoligong Fault, as the western boundary of the Indochina continent, moved in the dextral strike-slip on a large scale to trigger partial melting of the thickened crust, and the peraluminous granitic magma from which the Shuangmaidi two-mica orthoclase granite derived was formed.展开更多
The Middle-Late Jurassic Cu-Pb-Zn-bearing and W-bearing granites in the Nanling Range have distinctly different mineralogical and geochemical signatures. The Cu-Pb-Zn-bearing granites are dominated by metaluminous amp...The Middle-Late Jurassic Cu-Pb-Zn-bearing and W-bearing granites in the Nanling Range have distinctly different mineralogical and geochemical signatures. The Cu-Pb-Zn-bearing granites are dominated by metaluminous amphibole-bearing granodiorites, which have higher CaO/(Na2O+K2O) ratios, light/heavy rare earth element(LREE/HREE) ratios, and δEu values,lower Rb/Sr ratios, and weak Ba, Sr, P, and Ti depletions, exhibiting low degrees of fractionation. The W-bearing granites are highly differentiated and peraluminous, and they have lower CaO/(Na2O+K2O) ratios, LREE/HREE ratios, and δEu values,higher Rb/Sr ratios, and strong Ba, Sr, P, and Ti depletions. The Cu-Pb-Zn-bearing granites were formed predominantly between155.2 and 167.0 Ma with a peak value of 160.6 Ma, whereas the W-bearing granites were formed mainly from 151.1 to 161.8Ma with a peak value of 155.5 Ma. There is a time gap of about 5 Ma between the two different types of ore-bearing granites.Based on detailed geochronological and geochemical studies of both the Tongshanling Cu-Pb-Zn-bearing and Weijia W-bearing granites in southern Hunan Province and combined with the other Middle-Late Jurassic Cu-Pb-Zn-bearing and W-bearing granites in the Nanling Range, a genetic model of the two different types of ore-bearing granites has been proposed. Asthenosphere upwelling and basaltic magma underplating were induced by the subduction of the palaeo-Pacific plate. The underplated basaltic magmas provided heat to cause a partial melting of the mafic amphibolitic basement in the lower crust, resulting in the formation of Cu-Pb-Zn mineralization related granodioritic magmas. With the development of basaltic magma underplating,the muscovite-rich metasedimentary basement in the upper-middle crust was partially melted to generate W-bearing granitic magmas. The compositional difference of granite sources accounted for the metallogenic specialization, and the non-simultaneous partial melting of one source followed by the other brought about a time gap of about 5 Ma between the Cu-Pb-Zn-bearing and W-bearing granites.展开更多
The Zhuxi ore deposit is a super-large scheelite(copper) polymetallic deposit discovered in recent years. It grew above copper/tungsten-rich Neoproterozoic argilloarenaceous basement rocks and was formed in the contac...The Zhuxi ore deposit is a super-large scheelite(copper) polymetallic deposit discovered in recent years. It grew above copper/tungsten-rich Neoproterozoic argilloarenaceous basement rocks and was formed in the contact zone between Yanshanian granites and Carboniferous-Permian limestone. Granites related to this mineralization mainly include equigranular, middle- to coarse-grained granites and granitic porphyries. There are two mineralization types: skarn scheelite(copper) and granite scheelite mineralization. The former is large scale and has a high content of scheelite, whereas the latter is small scale and has a low content of scheelite. In the Taqian-Fuchun Basin, its NW boundary is a thrust fault, and the SE boundary is an angular unconformity with Proterozoic basement. In Carboniferous-Permian rock assemblages, the tungsten and copper contents in the limestone are both very high. The contents of major elements in granitoids do not differ largely between the periphery and the inside of the Zhuxi ore deposit. In both areas, the values of the aluminum saturation index are A/CNK>1.1, and the rocks are classified as potassium-rich strongly peraluminous granites. In terms of trace elements, compared to granites on the periphery of the Zhuxi ore deposit, the granites inside the Zhuxi ore deposit have smaller d Eu values, exhibit a significantly more negative Eu anomaly, are richer in Rb, U, Ta, Pb and Hf, and are more depleted in Ba, Ce, Sr, La and Ti, which indicates that they are highly differentiated S-type granites with a high degree of evolution. Under the influence of fluids, mineralization of sulfides is evident within massive rock formations inside the Zhuxi ore deposit, and the mean SO_3 content is 0.2%. Compared to peripheral rocks, the d Eu and total rare earth element(REE) content of granites inside the Zhuxi ore deposit are both lower, indicating a certain evolutionary inheritance relationship between the granites on the periphery and the granites inside the Zhuxi ore deposit. For peripheral and ore district plutons, U-Pb zircon dating shows an age range of 152–148 Ma. In situ Lu-Hf isotope analysis of zircon in the granites reveals that the calculated e_(Hf)(t) values are all negative, and the majority range from -6 to -9. The T_(DM2) values are concentrated in the range of 1.50–1.88 Ga(peak at 1.75 Ga), suggesting that the granitic magmas are derived from partial melting of ancient crust. This paper also discusses the metallogenic conditions and ore-controlling conditions of the ore district from the perspectives of mineral contents, hydrothermal alteration, and ore-controlling structures in the strata and the ore-bearing rocks. It is proposed that the Zhuxi ore deposit went through a multistage evolution, including oblique intrusion of granitic magmas, skarn mineralization, cooling and alteration, and precipitation of metal sulfides. The mineralization pattern can be summarized as "copper in the east and tungsten in the west, copper at shallow-middle depths and tungsten at deep depths, tungsten in the early stage and copper in the late stage".展开更多
基金Supported by Foundation for Excellent Youth Scholars of CAREERI,CAS(51Y184991)~~
文摘[Objective] The aim was to explore the relationship of pollen types and blooming plants,pollen fertility and meteorological factors during the falling process of pollen in the atmosphere.[Method] The falling record of pollen in Lanzhou University in spring in 2009 was obtained by 10 m platform natural falling method.The pollen falling number,duration,relationship among falling types and surrounding plants,as well as weather were discussed.[Result] Main pollen types included Populus L.,Salix,Paulownia and Platanaceae.Its pollen falling number,duration was consistent with the flowering period of corresponding plants in the garden;the classification and richness of falling of pollen was influenced by wind.The larger the wind was,the richer the variety become.There was basically no pollen from outside.The authors failed to observe the distinct changes of palynological assemblage in sandy storm weather.[Conclusion] The study could lay basis for the study of the modern progress of natural plants pollen.
文摘Based on field investigation and study of petrology, mineralogy, geochemistry and isotopic geochronology for the granodiorite, the authors discussed the formation setting of the granodiorite. The pluton is calci alkalic and peraluminous granite in which light rare earth elements (LREE) are intensively enriched, heavy rare earth elements are relatively poor, the ratio of LREE to heavy rare earth elements (HREE) is 7.12, and the fractionation of REE is good. The pluton formed in late orogenic setting which is relatively steady.
文摘AIM: To investigate whether birch pollen allergy symptoms are linked with gut microbiota changes and whether probiotics have an effect on these. METHODS: Forty seven children with confirmed birch pollen allergy were randomized to receive either a probiotic combination of Lactobacillus acidophilus (L. acidophilus) NCFM^TM (ATCC 700396) and Bifidobacterium lactis (B. lactis) BI-04 (ATCC SD5219) or placebo in a double-blind manner for 4 mo, starting prior to onset of the birch pollen season. Symptoms were recorded in a diary. Blood samples were taken for analysis of cytokines and eosinophils. Fecal samples were analysed for microbiota components, calprotectin and IgA. Nasal swabs were taken for analysis of eosinophils.RESULTS: The pollen season induced a reduction in Bifldobacterium , Clostridium and Bacteroides which could not be prevented by the probiotic intervention. During the intervention, significantly higher numbers ofB. lactis 11.2 × 10^7 ± 4.2 ×10^7 vs 0.1 × 10^7 ± 0.1 × 10^7 bacteria/g feces (P 〈 0.0001) and L. acidophilus NCFMTM 3.5 × 10^6 ± 1.3 × 10^6 vs 0.2 × 10^6 ±0.1 × 10^6 bacteria/g feces (P 〈 0.0001) were observed in the probiotic group compared to the placebo group.During May, there was a tendency for fewer subjects, (76.2% vs 95.2%, P = 0.078) to report runny nose, while during June, fewer subjects, 11.1% vs 33.3%, reported nasal blocking in the probiotics group (P = 0.101). Concomitantly, fewer subjects in the probiotic group had infiltration of eosinophils in the nasal mucosa compared to the placebo group, 57.1% vs 95% (P = 0.013). Eye symptoms tended to be slightly more frequent in the probiotic group, 12.5 d [interquartile range (IQR) 6-18] vs 7.5 d (IQR 0-11.5) (P = 0.066) during May. Fecal IgA was increased in the placebo group during the pollen season; this increase was prevented by the probiotics (P = 0.028). CONCLUSION: Birch pollen allergy was shown to be associated with changes in fecal microbiota composition. The specific combination of probiotics used was shown to prevent the pollen-induced infiltration of eosinophils into the nasal mucosa, and indicated a trend for reduced nasal symptoms.
基金supported in part by National Natural Science Foundation of China(61272148,61572525,61502056,and 61602525)Hunan Provincial Natural Science Foundation of China(2015JJ3010)Scientific Research Fund of Hunan Provincial Education Department(15B009,14C0285)
文摘The growing trend of network virtualization results in a widespread adoption of virtual switches in virtualized environments. However, virtual switching is confronted with great performance challenges regarding packet classification especially in Open Flow-based software defined networks. This paper first takes an insight into packet classification in virtual Open Flow switching, and points out that its performance bottleneck is dominated by flow table traversals of multiple failed mask probing for each arrived packet. Then we are motivated to propose an efficient packet classification algorithm based on counting bloom filters. In particular, counting bloom filters are applied to predict the failures of flow table lookups with great possibilities, and bypass flow table traversals for failed mask probing. Finally, our proposed packet classification algorithm is evaluated with real network traffic traces by experiments. The experimental results indicate that our proposed algorithm outperforms the classical one in Open v Switch in terms of average search length, and contributes to promote virtual Open Flow switching performance.
文摘Four concentrations of CCC were used to treat pepper seedlings.The results indicated that 50-150mg·L-1 CCC decreased the content of malonaldehydic acid (MDA) and increased the content of soluble protein,ascorbic acid (AsA) and glutathion(GSH),and activity of superoxide dismutase (SOD) and catalase (CAT),whereas 200mg·L-1 CCC caused the soluble protein content and the CAT activity of Xiangyan 1 and the AsA content of Xiangyan 10 to descend.The comprehensive evaluation manifested that 100-150mg·L-1 CCC had obvious effects on water-logging resistance of pepper.Different varieties required different concentrations,eg,l00mg·L-1 CCC suited Xiangyan 1,while 150 mg·L-1 CCC suited Xiangyan 10.
基金Project supported by the Knowledge Innovation Program of the Institute of Soil Science, Chinese Academy of Sciences,and the National Natural Science Foundation of China (No. 30270789).
文摘Most research in the past using genetically modified crops (GM crops) has focused on the ecological safety of foreign gene (i.e., the gene flow), gene products (for example, Bt (Bacillus thuringiensis) protein), and the safety of transgenic food for humans. In this study, changes in both the species and amounts of low-molecular-weight components in cotton (Gossypium hirsutum L.) root exudates after foreign Bt gene overexpression were investigated under different nutritional conditions. Transgenic cotton containing Bt (Bt-cotton), supplemented with all the mineral nutrients, secreted more organic acids than the wild-type cotton (WT). When nitrogen was removed from the full-nutrient solution, the amount of organic acids secretion of Bt-cotton was lesser than that of WT. The roots of the transgenic cotton secreted lesser amounts of amino acids and soluble sugars than the WT roots in the full-nutrient solution. Deficiencies of P and K caused a large increase in the total amino acid and soluble sugar secretions of both Bt-cotton and WT, with larger increases observed in Bt-cotton. Because transferring the foreign Bt gene into cotton can result in alterations in the components of the root exudates, with the effect varying depending on the nutritional status, the cultivation of genetically modified crops, such as Bt-cotton, in soil environments should be more carefully assessed, and the possible effects as a result of the alterations in the root exudate components should be considered.
基金Acknowledgments We thank ProfYongjun Liu, Dangsheng Li and Yangxin Fu for helpful comments and Dr Sheri Skinner for reviewing the manuscript and for constructive suggestions. This work was supported by grants from the National Natural Science Foundation of China (30530700, 30623003, 30600568, 30721065, 90713044, 30600308, 30801011, 30870126) and CAS project (KSCX1-YW-R-43), grant from SIBS project (2007KIP301), grants from the Ministry of Science and Technology (2006CB504300, 2007CB512404, 2006AA02A247, 20072714), the Technology Commission of Shanghai Municipality (88014199, 07DZ22916, 07XD14033, 064319034, 08431903004, 2008ZX10206, 08DZ2291703), EU project (FP6-2005-SSP-5-B, SP5B-CT-2006-044161) and from the E-institutes of Shanghai Universities Immunology Division.
文摘It is important to understand the pathogenesis of asthma induced by natural allergens, which could exclude the interference of artificial adjuvant and provide insights of natural immune response in the disease. In the present study, we show that Trichosanthin (TCS) could induce airway inflammation even without the help of alum. Furthermore, TCS appeared capable of replacing alum to promote OVA-specific airway inflammation. TCS induced accumulation of IL-4-producing eosinophils in peritoneum at an early stage and the adjuvant function of TCS was eliminated by blockage of IL-4 at this stage. Finally, the eosinophils triggered by TCS from WT mice, but not from IL-4- deficient mice were shown to function as adjuvant for the induction of OVA-specific Th2 responses. Our data indicate that TCS is not only an allergen, but also a Th2-typc adjuvant modulating the switching of immune responses to a Th2 pathway. This chain of events results from IL-4 production by eosinophils at an early stage of TCS-priming. In conclusion, TCS may be useful as a Th2 adjuvant, and innate immune cells, such as eosinophils, may be a good target to study the initiation of Th2 response.
文摘During three years lasting studies on the territory of the city of Novi Sad, terrain studies of Ambrosia artemisiifolia L. distribution as well as mapping were performed on regulated and disordered green areas and on arable areas. For mapping of ragweed distribution, partially modified method of Braun-Blanquet was used, and data processing was perfomed by specially designed program Ambrosia Spot Marker. Monitoring of this species was also done because of the occurrence of retrovegetation after mowing. During vegetation period, this allergenic weed species was controlled by multiple mowing, and rough ruderal sites outside settlements were treated by glyphosate at a rate of 1.2 kg ha^-1 -2.4 kg ha^-1. The study comprehended also monitoring of pollen concentrations in the air by Rotorod pollen sampler spore collector. A. artemisiifolia L. was established in 21 city zones on over 200 localities, on the banks of the river Danube, in Petrovaradin and Sremski Karlovci. Recommended mechanical measures such as mowing, drilling in the phase of germination, and chemical control measures, resulted in significant reduction ofA. artemisiifolia L. In the second half of the August 2009 the highest number of pollen grains was found in the amount of 783 pollen grains per m3 of air.
文摘Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent for extraction process. Besides functioning as dyes, anthocyanins have a role as a good antioxidant, so it can be used to protect the fat content of fermented milk such as yoghurt. This experiment was to find out the effect of different solvent type on extraction pigment (aquadest: sitrat acid/lactic acid/sulfate acid/chloride acid) and to determine the effect of the addition of anthocyanins in protecting the fat content of yogurt on several levels addition. The research result showed that anthocyanin's pigment of red rose from petal flower's could be effective solvent extracted using lactic acid 0.02 M and increased fat stability of yoghurt. The anthocyanin pigment showed with HPLC analysis that pigment resulted TLC analysis at rite time 13.10 (as Cyanidin glucoside) and 22.61 (as Malvidin glucoside). Yoghurt that given anthocyanin pigment has proven that fat content can be maintained its 86.7% (fat content with 2% pigment as such as 0.117% and without pigment 0.087%) after being stored for 6 days at cold and room temperature.
文摘We examined IgE levels in 4,312 samples of umbilical blood taken from children born into families with a positive history of allergy in one or both parents from 1998-2015. At the age often days, those with high IgE were given Colinfant Newborn (a lyophilized non-pathogenic strain of E. coli) for one month, three times weekly. At 15 months and three years we investigated the levels of IgE, IgA and IgG, and the incidence of illness and allergy. The results revealed that allergy and high umbilical IgE is strongly linked with family history (p 〈 0.001). We also detected differences in seasonality, especially with regards to pollen allergies. About 80% of children treated with Colinfant had significantly reduced IgE and morbidity at 13-15 months and 3 years, and furthermore without any clinical signs of allergy. Surprisingly 20% of treated children did not have reduced IgE, and yet they did not have any clinical signs of allergy. Normalization of IgA and IgG was seen in 90% of treated subjects (p 〈 0.001). These levels significantly are correlated with an almost negligible morbidity up to 4 years of life. Our study strongly suggests a positive effect of physiological E. coli on the microbiome of children as evidenced by a significantly reduced incidence of allergy and morbidity when applied early in life.
文摘The objectives of this study are to research the relationship between pectin and the softening of peach by soaking in citric acid solutions for 24 h at 35 ℃, pressurizing for 30 rain at 500 MPa or heating for 10 min. Also, comparing high-pressure-induced jam (HP-jam) and heat-induced jam (H-jam) were evaluated. It was found that firmness of the peach decreased greatly when soaked at pH 2.0 〉 heated 〉 soaked at pH 2.2 or 2.5 〉 pressurized, respectively. About 88% of the peach pectin was water-soluble-pectin and high-methoxyl pectin, while low-methoxyl pectin was slight. During pressurization, the pectin did not change. However, pectin degraded through hydrolysis during heating; consequently, the middle lamella separated. Also, eight kinds of peach jam (65% sugar, pH 2.0 or pH 2.2, and 50% or 60% sugar, pH 2.5) were compared. Both color and flavor of HP-jam were better than H-jam. As the pH values were lower, L-, a-, b-values of jam became higher, and the jam became pinker. Raw peach contained about 0.3%-0.4% pectin, therefore, an addition of 0.6% pectin was needed for both HP- and H-jams. However, there was no great difference in rheology or sensory evaluation between HP- and H-jams.
文摘The industrial chemistry of oils and fats is a mature technology, with decades of experience and refinement behind current practices, therefore some physico-chemical characteristics of eleven vegetable oils sunflower oil (four different manufacturing companies), olive oil (three different manufacturing companies), corn oil, castor oil, coconut oil and canola oil in Iraqi Kurdistan region (Hawler city center) have been evaluated according to association of official analytical chemists (AOAC) (1995) official methods. The refractive index, viscosity, acid value, peroxide value, pH values, and values of specific gravity of the vegetable oil samples were measured at various different conditions. The results showed that some of the oil samples have unacceptable values in comparison with the standards.
文摘The aim of this study was to determine the characteristics of the distribution of energy plant moisture content along the height of their shoots and the dynamics of moisture during storage in natural conditions. The shoots of Spartina, Miscanthus and willow were used in the study. Entire shoots were cut into sections of 10 cm and for each set in monthly cycles for six months moisture content was evaluated. After a month's storage of freshly cut shoots the biggest decrease of content moisture in the shoots of Spartina and Miscanthus was recorded, by 31% and 22%, respectively, and the lowest in willow shoots (12%). After sixth months of shoots storage the lowest moisture content (10%-12%) was reached in miscanthus. The most uneven moisture content along the height of shoots Spartina was characterized because on one third of the height from the bottom, the moisture content of shoots was 20%, and the top had moisture content in the range 5%-10%. Willow shoots were characterized by the smallest drop in moisture, and the final moisture content was about 23%, with the top part of moisture content of 10%-20%. The dynamics of moisture change during the six months of storage of grass shoots (Miscanthus and Spartina) in natural conditions under roofing was described by one power function regression, and willow by another one. Empirical models can be used to predict changes in moisture content of these plants in experiment conditions, since the coefficients of determination were 94.66% and 89.18%, respectively.
文摘This work aims to study the development of the pericarp of the fruit of Elaeis guineensis Jacq. var. dura. The thickness, the water and the oil contents of its tissues are evaluated every two weeks, from pollination to the maturity of the fruit. The development of the oil palm fruit takes 5.5 months. The endocarp reaches its maximum thickness at the 70th DPP (day post-pollination), with a water content of 72%. It then starts its dehydration, while sclerifying. It therefore isolates the seed at start and later protects it. The mesocarp is visible at anthesis and its water content is close to 92%. From the 100th DPP, it begins a continuous dehydration associated, from the 130th DPP, with an active lipids biosynthesis. Ultimately, the pericarp of the oil palm fruit fulfills both functions, namely to protect the seed by early sclerification of the endocarp and ensure the dissemination of the species by the high oil content of the mesocarp. A comparative anatomy of the pericarp tissues of the three genotypes.of E. guineensis Jacq., during the first three weeks of fruit development, will enhance the understanding of the primary effect of sh gene.
基金Supported by National Vegetable Industry System Construction
文摘[Objective] The aim was to provide certain theoretical basis for the protection of broccoli. [Method] Exogenous sodium nitroprusside was used to in the experiment. Under the condition of 20 ℃ and RH 95%, the optimal SNP concentration of broccoli was determined. [Result] The exogenous SNP could effectively maintain the quality of broccoli, inhabited the yellowing of broccoli and the increasing of cell membrane permeability, and maintain the soluble solids content, decreased the degradation rate of chlorophyll and delayed the loss of VC. [Conclusion] Exogenous sodium nitroprusside can effectively maintain the quality of broccoli to keep it fresh.
基金supported by National Natural Science Foundation of China (GrantNos. 40772197,40972232)National High-Tech Research & Development (Grant No. 2006BAB01A03)National Support Program of Science & Technology (Grant Nos. 2006BAB01A01,2006BAB01A03)
文摘The Baoshan Block is tectonically located in the middle segment of the Sibumasu plate. Granitic magmatism within the Baoshan Block has been considered weakly active due mainly to very limited exposures during the Himalaya orogenic episode. The geochronological study on the buried Shuangmaidi granite has confirmed the existence of the Cenozoic granitoids in the Baoshan Block. The present study indicates that: (1) It is medium- to coarse-grained two mica phyric granite, characterized by high SiO2 (73.55%-77.16%) and low CaO (0.34%-1.38%) contents, with a total alkalis (K2O+Na2O) of 5.22%-8.03%, K2O/Na2O ratios of 0.24-1.79, and total rare earth elements (ZREE) of the granite between 85 and 125 ppb. All samples are enriched in light REE and exhibit medium negative Eu anomalies; and they show pronounced negative anomalies in Ba, Sr, Ti, and Nb but significant positive anomalies in K, Rb, U, Th, and Pb on mantle-normalized trace element patterns, indicating typi-cally peraluminous to strongly peraluminous S-type granite. (2) The zircon SHRIMP U-Pb ages of the granite are 36.27±0.48 Ma for the samples from ZK7-1 and 35.78±0.49 Ma for those from ZK0-1, respectively. The similar zircon ages from these two drill cores may suggest that the granite samples come from the same buried pluton. (3) 206pb/204pb values of the granite vary from 20.115 to 25.359, 207pb/204pb from 15.776 to 16.160, and 208pb/204pb from 39.236 to 41.285, showing the characteristics of radio- active lead anomaly of the upper crust. The (87Sr/86Sr)i values calculated on the average age of the two-mica orthoclase granite (36 Ma) range from 0.72524 to 0.77503 and eNd(t) values vary from -10.9 to -11.7. These data, along with the depleted-mantle Nd modal ages of 1.73-1.80 Ga, imply that the granites might have formed from partial melting of the Precambrian crystal basements. (4) On the Hf-Rb-Ta diagram, almost all the samples fall within the field of post-collision tectonic setting. The CaO/Na2O and A1203/TiO2 ratios suggest that the granitic magma may have formed from partial melting of clay-rich crustal materials with a pos- sible melting temperature of about 900℃ and a possible crystallization temperature of 775-795~C. (5) During the post-collision of the Himalaya orogen, with the southeastward extrusion of the Indochina continent resulting from the continuous northward indentation of the India continent into the Asia, the Gaoligong Fault, as the western boundary of the Indochina continent, moved in the dextral strike-slip on a large scale to trigger partial melting of the thickened crust, and the peraluminous granitic magma from which the Shuangmaidi two-mica orthoclase granite derived was formed.
基金supported by the National Natural Science Foundation of China (Grant No. 41273053)the National Key Basic Research Program of China (Grant No. 2012CB416702)the Sino-French Cai Yuanpei Program of China Scholarship Council
文摘The Middle-Late Jurassic Cu-Pb-Zn-bearing and W-bearing granites in the Nanling Range have distinctly different mineralogical and geochemical signatures. The Cu-Pb-Zn-bearing granites are dominated by metaluminous amphibole-bearing granodiorites, which have higher CaO/(Na2O+K2O) ratios, light/heavy rare earth element(LREE/HREE) ratios, and δEu values,lower Rb/Sr ratios, and weak Ba, Sr, P, and Ti depletions, exhibiting low degrees of fractionation. The W-bearing granites are highly differentiated and peraluminous, and they have lower CaO/(Na2O+K2O) ratios, LREE/HREE ratios, and δEu values,higher Rb/Sr ratios, and strong Ba, Sr, P, and Ti depletions. The Cu-Pb-Zn-bearing granites were formed predominantly between155.2 and 167.0 Ma with a peak value of 160.6 Ma, whereas the W-bearing granites were formed mainly from 151.1 to 161.8Ma with a peak value of 155.5 Ma. There is a time gap of about 5 Ma between the two different types of ore-bearing granites.Based on detailed geochronological and geochemical studies of both the Tongshanling Cu-Pb-Zn-bearing and Weijia W-bearing granites in southern Hunan Province and combined with the other Middle-Late Jurassic Cu-Pb-Zn-bearing and W-bearing granites in the Nanling Range, a genetic model of the two different types of ore-bearing granites has been proposed. Asthenosphere upwelling and basaltic magma underplating were induced by the subduction of the palaeo-Pacific plate. The underplated basaltic magmas provided heat to cause a partial melting of the mafic amphibolitic basement in the lower crust, resulting in the formation of Cu-Pb-Zn mineralization related granodioritic magmas. With the development of basaltic magma underplating,the muscovite-rich metasedimentary basement in the upper-middle crust was partially melted to generate W-bearing granitic magmas. The compositional difference of granite sources accounted for the metallogenic specialization, and the non-simultaneous partial melting of one source followed by the other brought about a time gap of about 5 Ma between the Cu-Pb-Zn-bearing and W-bearing granites.
基金supported by the National Basic Research Program of China(Grant No.2012CB416701)National Natural Science Foundation of China(Grant Nos.41330208+3 种基金41572200)National Science and Technology Support Program(Grant No.2011BAB04B02)the Jiangxi Geological Exploration Fund(Grant No.20100112)Jiangxi Science and Technology Project(Grant No.20122BBG70068)
文摘The Zhuxi ore deposit is a super-large scheelite(copper) polymetallic deposit discovered in recent years. It grew above copper/tungsten-rich Neoproterozoic argilloarenaceous basement rocks and was formed in the contact zone between Yanshanian granites and Carboniferous-Permian limestone. Granites related to this mineralization mainly include equigranular, middle- to coarse-grained granites and granitic porphyries. There are two mineralization types: skarn scheelite(copper) and granite scheelite mineralization. The former is large scale and has a high content of scheelite, whereas the latter is small scale and has a low content of scheelite. In the Taqian-Fuchun Basin, its NW boundary is a thrust fault, and the SE boundary is an angular unconformity with Proterozoic basement. In Carboniferous-Permian rock assemblages, the tungsten and copper contents in the limestone are both very high. The contents of major elements in granitoids do not differ largely between the periphery and the inside of the Zhuxi ore deposit. In both areas, the values of the aluminum saturation index are A/CNK>1.1, and the rocks are classified as potassium-rich strongly peraluminous granites. In terms of trace elements, compared to granites on the periphery of the Zhuxi ore deposit, the granites inside the Zhuxi ore deposit have smaller d Eu values, exhibit a significantly more negative Eu anomaly, are richer in Rb, U, Ta, Pb and Hf, and are more depleted in Ba, Ce, Sr, La and Ti, which indicates that they are highly differentiated S-type granites with a high degree of evolution. Under the influence of fluids, mineralization of sulfides is evident within massive rock formations inside the Zhuxi ore deposit, and the mean SO_3 content is 0.2%. Compared to peripheral rocks, the d Eu and total rare earth element(REE) content of granites inside the Zhuxi ore deposit are both lower, indicating a certain evolutionary inheritance relationship between the granites on the periphery and the granites inside the Zhuxi ore deposit. For peripheral and ore district plutons, U-Pb zircon dating shows an age range of 152–148 Ma. In situ Lu-Hf isotope analysis of zircon in the granites reveals that the calculated e_(Hf)(t) values are all negative, and the majority range from -6 to -9. The T_(DM2) values are concentrated in the range of 1.50–1.88 Ga(peak at 1.75 Ga), suggesting that the granitic magmas are derived from partial melting of ancient crust. This paper also discusses the metallogenic conditions and ore-controlling conditions of the ore district from the perspectives of mineral contents, hydrothermal alteration, and ore-controlling structures in the strata and the ore-bearing rocks. It is proposed that the Zhuxi ore deposit went through a multistage evolution, including oblique intrusion of granitic magmas, skarn mineralization, cooling and alteration, and precipitation of metal sulfides. The mineralization pattern can be summarized as "copper in the east and tungsten in the west, copper at shallow-middle depths and tungsten at deep depths, tungsten in the early stage and copper in the late stage".