Genomic mining has identifi ed a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21（DE3） and further functionally characterized. Under optimal conditions（10 mmo...Genomic mining has identifi ed a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21（DE3） and further functionally characterized. Under optimal conditions（10 mmol/L substrate, p H 6.0, 2 h at 40 ℃）, this esterase can hydrolyze racemic methyl mandelate to（ R）-methyl mandelate with very high optical purity（e. e. 〉99%） and yield（nearly 90%）. Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate（ S）-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the fi nal chiral product（R）-methyl mandelate, which can potentially simplify the production procedure of（ R）-methyl mandelate catalyzed by esterase.展开更多
Aiming at the higher bit-rate occupation of motion vector encoding and more time load of full-searching strategies, a multi-resolution motion estimation and compensation algorithm based on adjacent prediction of frame...Aiming at the higher bit-rate occupation of motion vector encoding and more time load of full-searching strategies, a multi-resolution motion estimation and compensation algorithm based on adjacent prediction of frame difference was proposed.Differential motion detection was employed to image sequences and proper threshold was adopted to identify the connected region.Then the motion region was extracted to carry out motion estimation and motion compensation on it.The experiment results show that the encoding efficiency of motion vector is promoted, the complexity of motion estimation is reduced and the quality of the reconstruction image at the same bit-rate as Multi-Resolution Motion Estimation(MRME) is improved.展开更多
A novel moving object detection method was proposed in order to adapt the difficulties caused by intermittent object motion,thermal and dynamic background sequences.Two groups of complementary Gaussian mixture models ...A novel moving object detection method was proposed in order to adapt the difficulties caused by intermittent object motion,thermal and dynamic background sequences.Two groups of complementary Gaussian mixture models were used.The ghost and real static object could be classified by comparing the similarity of the edge images further.In each group,the multi resolution Gaussian mixture models were used and dual thresholds were applied in every resolution in order to get a complete object mask without much noise.The computational color model was also used to depress illustration variations and light shadows.The proposed method was verified by the public test sequences provided by the IEEE Change Detection Workshop and compared with three state-of-the-art methods.Experimental results demonstrate that the proposed method is better than others for all of the evaluation parameters in intermittent object motion sequences.Four and two in the seven evaluation parameters are better than the others in thermal and dynamic background sequences,respectively.The proposed method shows a relatively good performance,especially for the intermittent object motion sequences.展开更多
Video Super-Resolution （SR） reconstruction produces video sequences with High Resolution （HR） via the fusion of several Low-Resolution （LR） video frames. Traditional methods rely on the accurate estimation of su...Video Super-Resolution （SR） reconstruction produces video sequences with High Resolution （HR） via the fusion of several Low-Resolution （LR） video frames. Traditional methods rely on the accurate estimation of subpixel motion, which constrains their applicability to video sequences with relatively simple motions such as global translation. We propose an efficient iterative spatio-temporal adaptive SR reconstruction model based on Zemike Moment （ZM）, which is effective for spatial video sequences with arbitrary motion. The model uses region correlation judgment and self-adaptive threshold strategies to improve the effect and time efficiency of the ZM-based SR method. This leads to better mining of non-local self-similarity and local structural regularity, and is robust to noise and rotation. An efficient iterative curvature-based interpolation scheme is introduced to obtain the initial HR estimation of each LR video frame. Experimental results both on spatial and standard video sequences demonstrate that the proposed method outperforms existing methods in terms of both subjective visual and objective quantitative evaluations, and greatly improves the time efficiency.展开更多
Ultrafast electron diffraction （UED） is a rapidly advancing technique capable of recording the atomic-detail structural dynamics in real time. We report the establishment of the first UED system in China. Employing ...Ultrafast electron diffraction （UED） is a rapidly advancing technique capable of recording the atomic-detail structural dynamics in real time. We report the establishment of the first UED system in China. Employing this UED apparatus, both the coherent and the concurrent thermal lattice motions in an aluminium thin-film, trigged by ultrafast laser heating, have been observed. These results demonstrate its ability to directly measure a sub-milli-angstrom lattice spacing change on a sub-picosecond time scale.展开更多
The Yanchang Formation is extensively developed in the Ordos Basin and its surrounding regions. As one of the best terrestrial Triassic sequences in China and the major oil-gas bearing formations in the Ordos Basin, i...The Yanchang Formation is extensively developed in the Ordos Basin and its surrounding regions. As one of the best terrestrial Triassic sequences in China and the major oil-gas bearing formations in the Ordos Basin, its age determination and stratigraphic assignment are important in geological survey and oil-gas exploration. It had been attributed to the Late Triassic and regarded as the typical representative of the Upper Triassic in northern China for a long time, although some scholars had already proposed that the lower part of this formation should be of the Middle Triassic age in the mid-late 20 th century. In this paper, we suggest that the lower and middle parts of the Yanchang Formation should be of the Ladinian and the bottom possibly belongs to the late Anisian of the Middle Triassic, mainly based on new fossils found in it and high resolution radiometric dating results. The main source rocks, namely the oil shales and mudstones of the Chang-7, are of the Ladinian Age. The upper part of the Yanchang Formation, namely the Chang-6 and the above parts, belongs to the Late Triassic. The uppermost of the Triassic is missed in most parts of the Ordos Basin. The Middle-Upper Triassic Series boundary lies in the Yanchang Formation, equivalent to the boundary between Chang-7 and Chang-6. The Ladinian is an important palaeoenvironmental turning point in the Ordos Basin. Palaeoenvironmental changes in the basin are coincidence with that of the Sichuan Basin and the main tectonic movement of the Qinling Mountains. It indicates that tectonic activities of the Qinling Mountains are related to the big palaeoenvironmental changes in both the Ordos and Sichuan Basins, which are caused by the same structural dynamic system during the Ladinian.展开更多
基金Supported by the National Natural Science Foundation of China(No.21302199)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11030404)+1 种基金the Project of“Engineering HighPerformance Microorganisms for Advanced Bio-Based Manufacturing”from the Chinese Academy of Sciences(No.KGZD-EW-606)the Guangzhou Science and Technology Plan Projects(No.201510010012)
文摘Genomic mining has identifi ed a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21（DE3） and further functionally characterized. Under optimal conditions（10 mmol/L substrate, p H 6.0, 2 h at 40 ℃）, this esterase can hydrolyze racemic methyl mandelate to（ R）-methyl mandelate with very high optical purity（e. e. 〉99%） and yield（nearly 90%）. Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate（ S）-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the fi nal chiral product（R）-methyl mandelate, which can potentially simplify the production procedure of（ R）-methyl mandelate catalyzed by esterase.
基金Supported by the National Natural Science Foundation of China (No. 60803036)the Scientific Research Fund of Heilongjiang Provincial Education Department (No.11531013)
文摘Aiming at the higher bit-rate occupation of motion vector encoding and more time load of full-searching strategies, a multi-resolution motion estimation and compensation algorithm based on adjacent prediction of frame difference was proposed.Differential motion detection was employed to image sequences and proper threshold was adopted to identify the connected region.Then the motion region was extracted to carry out motion estimation and motion compensation on it.The experiment results show that the encoding efficiency of motion vector is promoted, the complexity of motion estimation is reduced and the quality of the reconstruction image at the same bit-rate as Multi-Resolution Motion Estimation(MRME) is improved.
基金Project(T201221207)supported by the Fundamental Research Fund for the Central Universities,ChinaProject(2012CB725301)supported by National Basic Research and Development Program,China
文摘A novel moving object detection method was proposed in order to adapt the difficulties caused by intermittent object motion,thermal and dynamic background sequences.Two groups of complementary Gaussian mixture models were used.The ghost and real static object could be classified by comparing the similarity of the edge images further.In each group,the multi resolution Gaussian mixture models were used and dual thresholds were applied in every resolution in order to get a complete object mask without much noise.The computational color model was also used to depress illustration variations and light shadows.The proposed method was verified by the public test sequences provided by the IEEE Change Detection Workshop and compared with three state-of-the-art methods.Experimental results demonstrate that the proposed method is better than others for all of the evaluation parameters in intermittent object motion sequences.Four and two in the seven evaluation parameters are better than the others in thermal and dynamic background sequences,respectively.The proposed method shows a relatively good performance,especially for the intermittent object motion sequences.
基金the National Basic Research Program of China (973 Program) under Grant No.2012CB821200，the National Natural Science Foundation of China under Grants No.91024001,No.61070142，the Beijing Natural Science Foundation under Grant No.4111002
文摘Video Super-Resolution （SR） reconstruction produces video sequences with High Resolution （HR） via the fusion of several Low-Resolution （LR） video frames. Traditional methods rely on the accurate estimation of subpixel motion, which constrains their applicability to video sequences with relatively simple motions such as global translation. We propose an efficient iterative spatio-temporal adaptive SR reconstruction model based on Zemike Moment （ZM）, which is effective for spatial video sequences with arbitrary motion. The model uses region correlation judgment and self-adaptive threshold strategies to improve the effect and time efficiency of the ZM-based SR method. This leads to better mining of non-local self-similarity and local structural regularity, and is robust to noise and rotation. An efficient iterative curvature-based interpolation scheme is introduced to obtain the initial HR estimation of each LR video frame. Experimental results both on spatial and standard video sequences demonstrate that the proposed method outperforms existing methods in terms of both subjective visual and objective quantitative evaluations, and greatly improves the time efficiency.
基金Supported by the National Natural Science Foundation of China under Grant Nos 10728409, 10734130, 10735050 and 60621063, and the National Basic Research Programme of China under Grant No 2007CB815102.
文摘Ultrafast electron diffraction （UED） is a rapidly advancing technique capable of recording the atomic-detail structural dynamics in real time. We report the establishment of the first UED system in China. Employing this UED apparatus, both the coherent and the concurrent thermal lattice motions in an aluminium thin-film, trigged by ultrafast laser heating, have been observed. These results demonstrate its ability to directly measure a sub-milli-angstrom lattice spacing change on a sub-picosecond time scale.
基金supported by PetroChina Basic Research Programs (Grant Nos. 2011A0206, 2014A-0216)the National Basic Research Program of China (Grant No. 2014CB239001)
文摘The Yanchang Formation is extensively developed in the Ordos Basin and its surrounding regions. As one of the best terrestrial Triassic sequences in China and the major oil-gas bearing formations in the Ordos Basin, its age determination and stratigraphic assignment are important in geological survey and oil-gas exploration. It had been attributed to the Late Triassic and regarded as the typical representative of the Upper Triassic in northern China for a long time, although some scholars had already proposed that the lower part of this formation should be of the Middle Triassic age in the mid-late 20 th century. In this paper, we suggest that the lower and middle parts of the Yanchang Formation should be of the Ladinian and the bottom possibly belongs to the late Anisian of the Middle Triassic, mainly based on new fossils found in it and high resolution radiometric dating results. The main source rocks, namely the oil shales and mudstones of the Chang-7, are of the Ladinian Age. The upper part of the Yanchang Formation, namely the Chang-6 and the above parts, belongs to the Late Triassic. The uppermost of the Triassic is missed in most parts of the Ordos Basin. The Middle-Upper Triassic Series boundary lies in the Yanchang Formation, equivalent to the boundary between Chang-7 and Chang-6. The Ladinian is an important palaeoenvironmental turning point in the Ordos Basin. Palaeoenvironmental changes in the basin are coincidence with that of the Sichuan Basin and the main tectonic movement of the Qinling Mountains. It indicates that tectonic activities of the Qinling Mountains are related to the big palaeoenvironmental changes in both the Ordos and Sichuan Basins, which are caused by the same structural dynamic system during the Ladinian.
