The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm mot...The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.展开更多
A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environment...A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environments. At the mobile hosts, all transactions perform local pre-validation. The local pre-validation process is carried out against the committed transactions at the server in the last broadcast cycle. Transactions that survive in local pre-validation must be submitted to the server for local final validation. The new protocol eliminates conflicts between mobile read-only and mobile update transactions, and resolves data conflicts flexibly by using multiversion dynamic adjustment of serialization order to avoid unnecessary restarts of transactions. Mobile read-only transactions can be committed with no-blocking, and respond time of mobile read-only transactions is greatly shortened. The tolerance of mobile transactions of disconnections from the broadcast channel is increased. In global validation mobile distributed transactions have to do check to ensure distributed serializability in all participants. The simulation results show that the new concurrency control protocol proposed offers better performance than other protocols in terms of miss rate, restart rate, commit rate. Under high work load (think time is ls) the miss rate of DMVOCC-MVDA is only 14.6%, is significantly lower than that of other protocols. The restart rate of DMVOCC-MVDA is only 32.3%, showing that DMVOCC-MVDA can effectively reduce the restart rate of mobile transactions. And the commit rate of DMVOCC-MVDA is up to 61.2%, which is obviously higher than that of other protocols.展开更多
The accuracy of a flight simulation is highly dependent on the quality of the aerodynamic database and prediction accuracies of the aerodynamic coefficients and derivatives. A surrogate model is an approximation metho...The accuracy of a flight simulation is highly dependent on the quality of the aerodynamic database and prediction accuracies of the aerodynamic coefficients and derivatives. A surrogate model is an approximation method that is used to predict unknown functions based on the sampling data obtained by the design of experiments. This model can also be used to predict aerodynamic coefficients/derivatives using several measured points. The objective of this paper is to develop an efficient digital flight simulation by solving the equation of motion to predict the aerodynamics data using a surrogate model. Accordingly, there is a need to construct and investigate aerodynamic databases and compare the accuracy of the surrogate model with the exact solution, and hence solve the equation of motion for the flight simulation analysis. In this study, sample datas for models are acquired from the USAF Stability and Control DATCOM, and a database is constructed for two input variables (the angle of attack and Mach number), along with two derivatives of the X-force axis and three derivatives for the Z-force axis and pitching moment. Furthermore, a comparison of the value predicted by the Kriging model and the exact solution shows that its flight analysis prediction ability makes it possible to use the surrogate model in future analyses.展开更多
Research at home and abroad shows that the simulation of ground motion using the 3D finite-difference method might be accurate and feasible. Based on related theories and methods,and using the wave velocity and densit...Research at home and abroad shows that the simulation of ground motion using the 3D finite-difference method might be accurate and feasible. Based on related theories and methods,and using the wave velocity and density model of the crust in the Yanqing-Huailai Basin,this paper makes a simulation of ground motion at Guanting Reservoir Dam based on the scenario earthquake in the Yanqing-Huailai Basin. Comparative analysis shows that the results of 3D finite-difference simulation accord with those of the empirical formula. The parameters such as the velocity-time series of ground motion,PGV and frequency might be referred to for the analysis of seismic protection design of the dam's structure.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31072212,41076100)the CAS Scientific and Technological Innovation Program for Cross and Cooperative Team,Marine Economy Innovative Development Project(No.12PYY001SF08)the National Key Basic Program of Science and Technology Platforms of Aquaculture Stock Resources,Shandong Technology Development Project(No.2013GHY11509)
文摘The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.
基金Project(20030533011)supported by the National Research Foundation for the Doctoral Program of Higher Education of China
文摘A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environments. At the mobile hosts, all transactions perform local pre-validation. The local pre-validation process is carried out against the committed transactions at the server in the last broadcast cycle. Transactions that survive in local pre-validation must be submitted to the server for local final validation. The new protocol eliminates conflicts between mobile read-only and mobile update transactions, and resolves data conflicts flexibly by using multiversion dynamic adjustment of serialization order to avoid unnecessary restarts of transactions. Mobile read-only transactions can be committed with no-blocking, and respond time of mobile read-only transactions is greatly shortened. The tolerance of mobile transactions of disconnections from the broadcast channel is increased. In global validation mobile distributed transactions have to do check to ensure distributed serializability in all participants. The simulation results show that the new concurrency control protocol proposed offers better performance than other protocols in terms of miss rate, restart rate, commit rate. Under high work load (think time is ls) the miss rate of DMVOCC-MVDA is only 14.6%, is significantly lower than that of other protocols. The restart rate of DMVOCC-MVDA is only 32.3%, showing that DMVOCC-MVDA can effectively reduce the restart rate of mobile transactions. And the commit rate of DMVOCC-MVDA is up to 61.2%, which is obviously higher than that of other protocols.
文摘The accuracy of a flight simulation is highly dependent on the quality of the aerodynamic database and prediction accuracies of the aerodynamic coefficients and derivatives. A surrogate model is an approximation method that is used to predict unknown functions based on the sampling data obtained by the design of experiments. This model can also be used to predict aerodynamic coefficients/derivatives using several measured points. The objective of this paper is to develop an efficient digital flight simulation by solving the equation of motion to predict the aerodynamics data using a surrogate model. Accordingly, there is a need to construct and investigate aerodynamic databases and compare the accuracy of the surrogate model with the exact solution, and hence solve the equation of motion for the flight simulation analysis. In this study, sample datas for models are acquired from the USAF Stability and Control DATCOM, and a database is constructed for two input variables (the angle of attack and Mach number), along with two derivatives of the X-force axis and three derivatives for the Z-force axis and pitching moment. Furthermore, a comparison of the value predicted by the Kriging model and the exact solution shows that its flight analysis prediction ability makes it possible to use the surrogate model in future analyses.
基金sponsored by the key lab.program of Shaanxi Province (08JZ04)the key discipline fund for scientific research program of Baoji University of Arts and Science (ZK0796)the key discipline fund for natural geography of Shaanxi Province in Baoji University of Arts and Science
文摘Research at home and abroad shows that the simulation of ground motion using the 3D finite-difference method might be accurate and feasible. Based on related theories and methods,and using the wave velocity and density model of the crust in the Yanqing-Huailai Basin,this paper makes a simulation of ground motion at Guanting Reservoir Dam based on the scenario earthquake in the Yanqing-Huailai Basin. Comparative analysis shows that the results of 3D finite-difference simulation accord with those of the empirical formula. The parameters such as the velocity-time series of ground motion,PGV and frequency might be referred to for the analysis of seismic protection design of the dam's structure.