Objective To examine the vesicular glutamate transporters （VGluTs： VGluT 1-VGluT3） in the peripheral vestibular system. Methods The vestibular structures, including Scarpa＇s ganglion （vestibular ganglion, VG）, m...Objective To examine the vesicular glutamate transporters （VGluTs： VGluT 1-VGluT3） in the peripheral vestibular system. Methods The vestibular structures, including Scarpa＇s ganglion （vestibular ganglion, VG）, maculae of utricle and saccule, and ampullary cristae, from normal Sprague-Dawley rats were processed immunohistochemically for VGluTs, by avidin-biotinylated peroxidase complex method, with 3-3＇-diaminobenzidine （DAB） as chromogen. Results （1） VGluT 1 was localized to partial neurons of VG and to the putative primary afferent fibers innervating vestibular end-organs. （2） Intense VGluT3 immunoreactivity was detected in large number of sensory epithelia cells, and weak labeling of VGluT3- positive afferent fibers was in the maculae and ampullary cristae. （3） No or very weak VGluT2 immunoreactivity was observed in the VG and acoustic maculae. Conclusion These results provide the morphological support that glutamate exists in the peripheral vestibular system, and it may play an important role in the centripetal vestibular transmission.展开更多
文摘Objective To examine the vesicular glutamate transporters （VGluTs： VGluT 1-VGluT3） in the peripheral vestibular system. Methods The vestibular structures, including Scarpa＇s ganglion （vestibular ganglion, VG）, maculae of utricle and saccule, and ampullary cristae, from normal Sprague-Dawley rats were processed immunohistochemically for VGluTs, by avidin-biotinylated peroxidase complex method, with 3-3＇-diaminobenzidine （DAB） as chromogen. Results （1） VGluT 1 was localized to partial neurons of VG and to the putative primary afferent fibers innervating vestibular end-organs. （2） Intense VGluT3 immunoreactivity was detected in large number of sensory epithelia cells, and weak labeling of VGluT3- positive afferent fibers was in the maculae and ampullary cristae. （3） No or very weak VGluT2 immunoreactivity was observed in the VG and acoustic maculae. Conclusion These results provide the morphological support that glutamate exists in the peripheral vestibular system, and it may play an important role in the centripetal vestibular transmission.
