采用美国沃特邦(BOWNT)公司生产的BOD SH 405/8FC型叠片式过滤器对进入超滤的生产水进行前置过滤,叠片式过滤器在运行3个月左右后压差普遍升高,导致超滤出水浊度偏高,经分析是由于沙子、铁锈垢堵塞叠片间的过滤沟槽和过滤器反洗参数设...采用美国沃特邦(BOWNT)公司生产的BOD SH 405/8FC型叠片式过滤器对进入超滤的生产水进行前置过滤,叠片式过滤器在运行3个月左右后压差普遍升高,导致超滤出水浊度偏高,经分析是由于沙子、铁锈垢堵塞叠片间的过滤沟槽和过滤器反洗参数设置不合理造成的。先通过水冲洗再通过酸洗去除叠片式内铁锈和缩短反洗时间间隔等措施,叠片式过滤器进出水管压差明显降低,出水浊度降低,过滤效果得到明显提升。展开更多
The transmission and dispersive characteristics of slotline are calculated in this paper. The tail of Gaussion pulse is improved because a modified dispersive boundary condition (DBC) is adopted. It leads to a reduct...The transmission and dispersive characteristics of slotline are calculated in this paper. The tail of Gaussion pulse is improved because a modified dispersive boundary condition (DBC) is adopted. It leads to a reduction in computer memory requirements and computational time. The computational domain is greatly reduced to enable performance in personal computer. At the same time because edges of a boundary and summits are treated well, the computational results is more accurate and more collector.展开更多
Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence...Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence in the untranslated regions. RT_PCR and Southern blotting using 3′_untranslated region (3′_UTR) as specific probe revealed that pea isoactin genes were expressed in roots, stems, leaves, tendrils, pollen and juvenile siliques, but displayed different patterns of transcript accumulation. Two_fold serial dilution electrophoresis showed PEAcⅠ mRNA preferentially accumulated in rapidly developing tissues: it peaked in seven days' stem; remained at a rather high level in leaves within a month but decreased significantly later; varied a little in tendrils and reached a median and a very low level respectively in juvenile siliques and in pollen. PEAcⅡ displayed somewhat similar expression pattern to PEAcⅠ. The observed differences in sequences and transcript accumulation patterns suggest that the individual pea actin genes may differ in their transcriptional regulation and cellular function. Phylogenetic tree of actins showed that pea actin isoforms are as diverged from each other as they are from other plant actins, and pea actins might have originated from a common ancestor before the divergence of the dicot and monocot plants.展开更多
Actin depolymerizing factor (ADF), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin fi...Actin depolymerizing factor (ADF), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin filaments. Four cDNAs (designated GhADF2, GhADF3, GhADF4, and GhADF5, respectively) encoding ADF proteins were isolated from cotton (Gossypium hirsutum) fiber cDNA library. GhADF2 cDNA is 705 bp in length and deduces a protein with 139 amino acids. GhADF3 cDNA is 819 bp in length and encodes a protein of 139 amino acids. GhADF4 cDNA is 804 bp in length and deduces a protein with 143 amino acids. GhADF5 cDNA is 644 bp in length and encodes a protein of 141 amino acids. The molecular evolutionary relationship of these genes was analyzed by means of bioinformatics. GhADF2 is closely related to GhADF3 (99% identity) and PetADF2 (89% identity). GhADF4 is closely related to AtADF6 (78% identity), and GhADF5 is closely related to AtADF5 (83% identity). These results demonstrated that the plant ADF genes are highly conserved in structure. RT-PCR analysis showed that GhADF2 is predominantly expressed in fiber, whereas, GhADF5 is mainly expressed in cotyledons. On the other hand, it seems that GhADF3 and GhADF4 have no tissue specificity. Expression levels of different ADF genes may vary considerably in the same cell type, suggesting that they might be involved in regulating tissue development of cotton and the each ADF isoform may diverge to form the functional difference from the other ADFs during evolution.展开更多
The mRNA Differential Display is a new molecular biological strategy for detecting and characterizing altered gene expression in eukaryotic cells which wad developed in 1992.Because of its simplicity,sensitivity and r...The mRNA Differential Display is a new molecular biological strategy for detecting and characterizing altered gene expression in eukaryotic cells which wad developed in 1992.Because of its simplicity,sensitivity and reproducibility,this method should find wide-ranging underpaid application developmental and molecular biology.Therecent successful applications of this method to gene hunting and the technological improvement promise great potential of mRNA Differential Display.展开更多
Differential evolution (DE) is an evolutionary optimization method, which has been successfully used in many practical cases. However, DE involves large computation time, especially, when used to optimize the compur...Differential evolution (DE) is an evolutionary optimization method, which has been successfully used in many practical cases. However, DE involves large computation time, especially, when used to optimize the compurationally expensive objective function. To overcome this .difficulty, the concept of immunity based on vaccination is used to help proliferate excellent schemata and to restrain the degenerate phenomenon. To improve the effective- ness of vaccines, a new vaccine autonomous obtaining method, and a method of deciding the probability of vacci- nation are proposed. In addition, a method for modifying the search space dynamically is proposed to enhance the possibility of converging to the true global optimum. Experiments showed that the improved DE performs better than the classical DE significantly.展开更多
A novel and simple technique to control the search direction of the differential mutation was proposed.In order to verify the performance of this method,ten widely used benchmark functions were chosen and the results ...A novel and simple technique to control the search direction of the differential mutation was proposed.In order to verify the performance of this method,ten widely used benchmark functions were chosen and the results were compared with the original differential evolution(DE)algorithm.Experimental results indicate that the search direction controlled DE algorithm obtains better results than the original DE algorithm in term of the solution quality and convergence rate.展开更多
A new version of differential evolution (DE) algorithm, in which immune concepts and methods are applied to determine the parameter setting, named immune self-adaptive differential evolution (ISDE), is proposed to...A new version of differential evolution (DE) algorithm, in which immune concepts and methods are applied to determine the parameter setting, named immune self-adaptive differential evolution (ISDE), is proposed to improve the performance of the DE algorithm. During the actual operation, ISDE seeks the optimal parameters arising from the evolutionary process, which enable ISDE to alter the algorithm for different optimization problems and improve the performance of ISDE by the control parameters' self-adaptation. The .performance of the proposed method is studied with the use of nine benchmark problems and compared with original DE algorithm ~nd-other well-known self-adaptive DE algorithms. The experiments conducted show that the ISDE clearly outperforms the other DE algorithms in all benchmark functions. Furthermore, ISDE is applied to develop the kinetic model for homogeneous mercury. (Hg) oxidation in flue gas, and satisfactory results are obtained.展开更多
文摘采用美国沃特邦(BOWNT)公司生产的BOD SH 405/8FC型叠片式过滤器对进入超滤的生产水进行前置过滤,叠片式过滤器在运行3个月左右后压差普遍升高,导致超滤出水浊度偏高,经分析是由于沙子、铁锈垢堵塞叠片间的过滤沟槽和过滤器反洗参数设置不合理造成的。先通过水冲洗再通过酸洗去除叠片式内铁锈和缩短反洗时间间隔等措施,叠片式过滤器进出水管压差明显降低,出水浊度降低,过滤效果得到明显提升。
文摘The transmission and dispersive characteristics of slotline are calculated in this paper. The tail of Gaussion pulse is improved because a modified dispersive boundary condition (DBC) is adopted. It leads to a reduction in computer memory requirements and computational time. The computational domain is greatly reduced to enable performance in personal computer. At the same time because edges of a boundary and summits are treated well, the computational results is more accurate and more collector.
文摘Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence in the untranslated regions. RT_PCR and Southern blotting using 3′_untranslated region (3′_UTR) as specific probe revealed that pea isoactin genes were expressed in roots, stems, leaves, tendrils, pollen and juvenile siliques, but displayed different patterns of transcript accumulation. Two_fold serial dilution electrophoresis showed PEAcⅠ mRNA preferentially accumulated in rapidly developing tissues: it peaked in seven days' stem; remained at a rather high level in leaves within a month but decreased significantly later; varied a little in tendrils and reached a median and a very low level respectively in juvenile siliques and in pollen. PEAcⅡ displayed somewhat similar expression pattern to PEAcⅠ. The observed differences in sequences and transcript accumulation patterns suggest that the individual pea actin genes may differ in their transcriptional regulation and cellular function. Phylogenetic tree of actins showed that pea actin isoforms are as diverged from each other as they are from other plant actins, and pea actins might have originated from a common ancestor before the divergence of the dicot and monocot plants.
基金This work was supported by the National Natural Sciences Foundation of China (No. 30470930)the Ministry of Education of China (No. 104130)the National Program for Basic Research and Development (973) of China (No. 2004CB117304).
文摘Actin depolymerizing factor (ADF), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin filaments. Four cDNAs (designated GhADF2, GhADF3, GhADF4, and GhADF5, respectively) encoding ADF proteins were isolated from cotton (Gossypium hirsutum) fiber cDNA library. GhADF2 cDNA is 705 bp in length and deduces a protein with 139 amino acids. GhADF3 cDNA is 819 bp in length and encodes a protein of 139 amino acids. GhADF4 cDNA is 804 bp in length and deduces a protein with 143 amino acids. GhADF5 cDNA is 644 bp in length and encodes a protein of 141 amino acids. The molecular evolutionary relationship of these genes was analyzed by means of bioinformatics. GhADF2 is closely related to GhADF3 (99% identity) and PetADF2 (89% identity). GhADF4 is closely related to AtADF6 (78% identity), and GhADF5 is closely related to AtADF5 (83% identity). These results demonstrated that the plant ADF genes are highly conserved in structure. RT-PCR analysis showed that GhADF2 is predominantly expressed in fiber, whereas, GhADF5 is mainly expressed in cotyledons. On the other hand, it seems that GhADF3 and GhADF4 have no tissue specificity. Expression levels of different ADF genes may vary considerably in the same cell type, suggesting that they might be involved in regulating tissue development of cotton and the each ADF isoform may diverge to form the functional difference from the other ADFs during evolution.
文摘The mRNA Differential Display is a new molecular biological strategy for detecting and characterizing altered gene expression in eukaryotic cells which wad developed in 1992.Because of its simplicity,sensitivity and reproducibility,this method should find wide-ranging underpaid application developmental and molecular biology.Therecent successful applications of this method to gene hunting and the technological improvement promise great potential of mRNA Differential Display.
基金Supported by the National Natural Science Foundation of China (60736021), the National High Technology Research and Development Program of China (2006AA04Z184, 2007AA041406), and the Key Technologies R&D Program of Zhejiang Province (2006C 11066, 2006C31051).
文摘Differential evolution (DE) is an evolutionary optimization method, which has been successfully used in many practical cases. However, DE involves large computation time, especially, when used to optimize the compurationally expensive objective function. To overcome this .difficulty, the concept of immunity based on vaccination is used to help proliferate excellent schemata and to restrain the degenerate phenomenon. To improve the effective- ness of vaccines, a new vaccine autonomous obtaining method, and a method of deciding the probability of vacci- nation are proposed. In addition, a method for modifying the search space dynamically is proposed to enhance the possibility of converging to the true global optimum. Experiments showed that the improved DE performs better than the classical DE significantly.
基金Project(2011FJ3016)supported by the Research Foundation of Science & Technology Office of Hunan Province,China
文摘A novel and simple technique to control the search direction of the differential mutation was proposed.In order to verify the performance of this method,ten widely used benchmark functions were chosen and the results were compared with the original differential evolution(DE)algorithm.Experimental results indicate that the search direction controlled DE algorithm obtains better results than the original DE algorithm in term of the solution quality and convergence rate.
基金Supported by the National Natural Science Foundation of China (20506003, 20776042) and the National High-Tech Research and Development Program of China (2007AA04Z 164).
文摘A new version of differential evolution (DE) algorithm, in which immune concepts and methods are applied to determine the parameter setting, named immune self-adaptive differential evolution (ISDE), is proposed to improve the performance of the DE algorithm. During the actual operation, ISDE seeks the optimal parameters arising from the evolutionary process, which enable ISDE to alter the algorithm for different optimization problems and improve the performance of ISDE by the control parameters' self-adaptation. The .performance of the proposed method is studied with the use of nine benchmark problems and compared with original DE algorithm ~nd-other well-known self-adaptive DE algorithms. The experiments conducted show that the ISDE clearly outperforms the other DE algorithms in all benchmark functions. Furthermore, ISDE is applied to develop the kinetic model for homogeneous mercury. (Hg) oxidation in flue gas, and satisfactory results are obtained.