In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to ration...In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein(QP-C)gene from the giant panda was analyzed.The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues.The pI of the protein is 10.50 and its mole cular weight is 9.60×103 Da.Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site.Comparing the cDNA sequence with amino acid sequence of giant panda’s QP-C gene and cDNA sequence with the amino acid sequence of other species’ QP-C gene shows high similarity.The similarity is 82.3% to 95.2% and 81.5% to 95.1%.展开更多
在已获得水稻泛素连接酶基因OsHUB2过表达转基因植株的基础上,根据OsHUB2基因序列设计3对引物,分别为OsHUB2-1、OsHUB2-2、OsHUB2-3,通过RT-PCR筛选条带特异单一的引物,real time PCR(qRT-PCR)方法验证最适合检测OsHUB2的反应条件。结...在已获得水稻泛素连接酶基因OsHUB2过表达转基因植株的基础上,根据OsHUB2基因序列设计3对引物,分别为OsHUB2-1、OsHUB2-2、OsHUB2-3,通过RT-PCR筛选条带特异单一的引物,real time PCR(qRT-PCR)方法验证最适合检测OsHUB2的反应条件。结果表明OsHUB2-F3R3扩增的PCR产物特异性最强,扩增曲线及熔解曲线等指标都在理想范围内,同时该引物能很好地区分对照及转基因材料,进而筛选出OsHUB2基因上调表达的转基因植株。Realtime体系的建立与优化为今后进一步研究水稻泛素连接酶基因OsHUB2的功能奠定了基础。展开更多
文摘In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein(QP-C)gene from the giant panda was analyzed.The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues.The pI of the protein is 10.50 and its mole cular weight is 9.60×103 Da.Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site.Comparing the cDNA sequence with amino acid sequence of giant panda’s QP-C gene and cDNA sequence with the amino acid sequence of other species’ QP-C gene shows high similarity.The similarity is 82.3% to 95.2% and 81.5% to 95.1%.
文摘在已获得水稻泛素连接酶基因OsHUB2过表达转基因植株的基础上,根据OsHUB2基因序列设计3对引物,分别为OsHUB2-1、OsHUB2-2、OsHUB2-3,通过RT-PCR筛选条带特异单一的引物,real time PCR(qRT-PCR)方法验证最适合检测OsHUB2的反应条件。结果表明OsHUB2-F3R3扩增的PCR产物特异性最强,扩增曲线及熔解曲线等指标都在理想范围内,同时该引物能很好地区分对照及转基因材料,进而筛选出OsHUB2基因上调表达的转基因植株。Realtime体系的建立与优化为今后进一步研究水稻泛素连接酶基因OsHUB2的功能奠定了基础。
