Objective: To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), the central enzymes in folate metabolism that affects DNA meth...Objective: To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), the central enzymes in folate metabolism that affects DNA methylation and synthesis, and the risk of Down syndrome in China. Methods: Genomic DNA was isolated from the peripheral lymphocytes of 64 mothers of children with Down syndrome and 70 age matched control subjects. Polymerase chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTHFR 677C→T, MTRR 66A→G and the relationship between these genotypes and the risk of Down syndrome was analyzed. Results: The results show that the MTHFR 677C→T polymorphism is more prevalent among mothers of children with Down syndrome than among control mothers, with an odds ratio of 3.78 (95% confidence interval (CI), 1.78～8.47). In addition, the homozygous MTRR 66A→G polymorphism was independently associated with a 5.2-fold increase in estimated risk (95% CI, 1.90～14.22). The combined presence of both polymorphisms was associated with a greater risk of Down syndrome than the presence of either alone, with an odds ratio of 6.0 (95% CI, 2.058～17.496). The two polymorphisms appear to act without a multiplicative interaction. Conclusion: MTHFR and MTRR gene mutation alleles are related to Down syndrome, and CT, TT and GG gene mutation types increase the risk of Down syndrome.展开更多
Ovoviviparous black rockfish(Sebastes schlegeli) is an important marine fish species for aquaculture and fisheries in China. Genetic information of this species is scarce because of the lack of microsatellite markers....Ovoviviparous black rockfish(Sebastes schlegeli) is an important marine fish species for aquaculture and fisheries in China. Genetic information of this species is scarce because of the lack of microsatellite markers. In this study, a large number of microsatellite markers of black rockfish were isolated by constructing microsatellite-enriched libraries. Female- and male-specific genetic linkage maps were constructed using 435 microsatellite markers genotyped in a full-sib family of the fish species. The female linkage map contained 140 microsatellite markers, in which 23 linkage groups had a total genetic length of 1334.1 c M and average inter-marker space of 13.3 c M. The male linkage map contained 156 microsatellite markers, in which 25 linkage groups had a total genetic length of 1359.6 c M and average inter-marker distance of 12.4 c M. The genome coverage of the female and male linkage maps was 68.6% and 69.3%, respectively. The female-to-male ratio of the recombination rate was approximately 1.07:1 in adjacent microsatellite markers. This paper presents the first genetic linkage map of microsatellites in black rockfish. The collection of polymorphic markers and sex-specific linkage maps of black rockfish could be useful for further investigations on parental assignment, population genetics, quantitative trait loci mapping, and marker-assisted selection in related breeding programs.展开更多
Here concerned is a certain kind of non-standard measure defined on the n-dimensional Euclidean space (Rn), which (with n = 1) can be used to show that any standard linear point-set or the usual ordered field R of rea...Here concerned is a certain kind of non-standard measure defined on the n-dimensional Euclidean space (Rn), which (with n = 1) can be used to show that any standard linear point-set or the usual ordered field R of real numbers is of measure zero. The proposition just mentioned is basically consistent with Poincare's famous remark which renders a deep insight into the paradoxical structural nature of Cantor's continuum consisting precisely of all distinct real numbers.展开更多
This paper studies denumerable continuous-time Markov decision processes with expected total reward criteria. The authors first study the unconstrained model with possible unbounded transition rates, and give suitable...This paper studies denumerable continuous-time Markov decision processes with expected total reward criteria. The authors first study the unconstrained model with possible unbounded transition rates, and give suitable conditions on the controlled system＇s primitive data under which the authors show the existence of a solution to the total reward optimality equation and also the existence of an optimal stationary policy. Then, the authors impose a constraint on an expected total cost, and consider the associated constrained model. Basing on the results about the unconstrained model and using the Lagrange multipliers approach, the authors prove the existence of constrained-optimal policies under some additional conditions. Finally, the authors apply the results to controlled queueing systems.展开更多
基金Project (No. 2003ABA148) supported by the Science Foundation of Hubei Province, China
文摘Objective: To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), the central enzymes in folate metabolism that affects DNA methylation and synthesis, and the risk of Down syndrome in China. Methods: Genomic DNA was isolated from the peripheral lymphocytes of 64 mothers of children with Down syndrome and 70 age matched control subjects. Polymerase chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTHFR 677C→T, MTRR 66A→G and the relationship between these genotypes and the risk of Down syndrome was analyzed. Results: The results show that the MTHFR 677C→T polymorphism is more prevalent among mothers of children with Down syndrome than among control mothers, with an odds ratio of 3.78 (95% confidence interval (CI), 1.78～8.47). In addition, the homozygous MTRR 66A→G polymorphism was independently associated with a 5.2-fold increase in estimated risk (95% CI, 1.90～14.22). The combined presence of both polymorphisms was associated with a greater risk of Down syndrome than the presence of either alone, with an odds ratio of 6.0 (95% CI, 2.058～17.496). The two polymorphisms appear to act without a multiplicative interaction. Conclusion: MTHFR and MTRR gene mutation alleles are related to Down syndrome, and CT, TT and GG gene mutation types increase the risk of Down syndrome.
基金supported by the National High-Tech Research and Development Program (Grant Nos.2012AA10A408 and 2012AA10A402)
文摘Ovoviviparous black rockfish(Sebastes schlegeli) is an important marine fish species for aquaculture and fisheries in China. Genetic information of this species is scarce because of the lack of microsatellite markers. In this study, a large number of microsatellite markers of black rockfish were isolated by constructing microsatellite-enriched libraries. Female- and male-specific genetic linkage maps were constructed using 435 microsatellite markers genotyped in a full-sib family of the fish species. The female linkage map contained 140 microsatellite markers, in which 23 linkage groups had a total genetic length of 1334.1 c M and average inter-marker space of 13.3 c M. The male linkage map contained 156 microsatellite markers, in which 25 linkage groups had a total genetic length of 1359.6 c M and average inter-marker distance of 12.4 c M. The genome coverage of the female and male linkage maps was 68.6% and 69.3%, respectively. The female-to-male ratio of the recombination rate was approximately 1.07:1 in adjacent microsatellite markers. This paper presents the first genetic linkage map of microsatellites in black rockfish. The collection of polymorphic markers and sex-specific linkage maps of black rockfish could be useful for further investigations on parental assignment, population genetics, quantitative trait loci mapping, and marker-assisted selection in related breeding programs.
基金Supperted by Special Foundation of Dalian Univ. of Technology.
文摘Here concerned is a certain kind of non-standard measure defined on the n-dimensional Euclidean space (Rn), which (with n = 1) can be used to show that any standard linear point-set or the usual ordered field R of real numbers is of measure zero. The proposition just mentioned is basically consistent with Poincare's famous remark which renders a deep insight into the paradoxical structural nature of Cantor's continuum consisting precisely of all distinct real numbers.
基金supported by the National Natural Science Foundation of China under Grant Nos.10925107 and 60874004
文摘This paper studies denumerable continuous-time Markov decision processes with expected total reward criteria. The authors first study the unconstrained model with possible unbounded transition rates, and give suitable conditions on the controlled system＇s primitive data under which the authors show the existence of a solution to the total reward optimality equation and also the existence of an optimal stationary policy. Then, the authors impose a constraint on an expected total cost, and consider the associated constrained model. Basing on the results about the unconstrained model and using the Lagrange multipliers approach, the authors prove the existence of constrained-optimal policies under some additional conditions. Finally, the authors apply the results to controlled queueing systems.
