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Online support vector regression for reinforcement learning
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作者 于振华 Cai Yuanli 《High Technology Letters》 EI CAS 2007年第2期173-176,共4页
The goal in reinforcement learning is to learn the value of state-action pair in order to maximize the total reward. For continuous states and actions in the real world, the representation of value functions is critic... The goal in reinforcement learning is to learn the value of state-action pair in order to maximize the total reward. For continuous states and actions in the real world, the representation of value functions is critical. Furthermore, the samples in value functions are sequentially obtained. Therefore, an online sup-port vector regression (OSVR) is set up, which is a function approximator to estimate value functions in reinforcement learning. OSVR updates the regression function by analyzing the possible variation of sup-port vector sets after new samples are inserted to the training set. To evaluate the OSVR learning ability, it is applied to the mountain-car task. The simulation results indicate that the OSVR has a preferable con- vergence speed and can solve continuous problems that are infeasible using lookup table. 展开更多
关键词 reinforcement learning function approximation support vector regression online leaming
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突变质粒CMV-3flag-hPROKR2(343YFK/AAA345,351HWR/AAA353)的构建及表达
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作者 周晓涛 周文涛 +6 位作者 彭震 刘化蝶 陈丹娜 夏开德 迪丽娜尔.波拉提 赵云娟 李家大 《生物技术》 CAS 2018年第2期113-118,150,共7页
[目的]构建CMV-3flag-h PROKR2(343YFK/AAA345,351HWR/AAA353)真核表达质粒并观察其蛋白表达。[方法]将NotⅠ的酶切位点(GCGGCCGCC)引入第一次PCR的后引物,扩增并突变h PROKR2的1-1035 bp(343YFK/AAA345),再将NotⅠ的酶切位点和3个连续... [目的]构建CMV-3flag-h PROKR2(343YFK/AAA345,351HWR/AAA353)真核表达质粒并观察其蛋白表达。[方法]将NotⅠ的酶切位点(GCGGCCGCC)引入第一次PCR的后引物,扩增并突变h PROKR2的1-1035 bp(343YFK/AAA345),再将NotⅠ的酶切位点和3个连续甘氨酸突变碱基GCCGCCGCA引入第二次PCR的前引物扩增并突变h PROKR2的1036-1152bp(351HWR/AAA353),通过NotⅠ酶切位点连接前后两个片段,即可构建pc DNA3.1-h PROKR2-myc-his(343YFK/AAA345,351HWR/AAA353),最后通过测序鉴定突变是否成功。扩增h PROKR2(343YFK/AAA345,351HWR/AAA353),连接入CMV-3flag。Western Blot检测相应蛋白的表达。[结果]成功构建CMV-3flag-h PROKR2(343YFK/AAA345,351HWR/AAA353)质粒,并验证到相应蛋白表达。[结论]利用NotⅠ酶的碱基序列特性(GCGGCCGCC)进行3个连续氨基酸突变的方法简单有效。质粒CMV-3flag-h PROKR2(343YFK/AAA345,351HWR/AAA353)的成功构建为后期进一步实验创造条件。 展开更多
关键词 hPROKR2 连续位点碱基突变 限制性内切酶NotⅠ 质粒构建
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