A novel continuous-flow PCR chip adopting self-heating, passive-cooling mode to realize the DNA fragments amplification was presented. Using the ANSYS finite element analysis, the temperature distribution of the chip ...A novel continuous-flow PCR chip adopting self-heating, passive-cooling mode to realize the DNA fragments amplification was presented. Using the ANSYS finite element analysis, the temperature distribution of the chip is simulated and analyzed.The optimal size of the chip is 30×22 mm2, the roundabout micro-channel is the 90 μm width, 40 μm depth. Two micro-heater with the nickel-chrome alloy material film are formed on the side of silicon belonging to denaturation and renaturation zones needed for PCR reaction, and two adiabatic structures with groove on side of silicon by anisotropy etching. By the mode of heating local zones at single side, three wider constant temperature zones could be formed, which are 60 ℃,72 ℃,95 ℃ and suitable for PCR,and the temperature-difference could be restricted in less than 5 ℃.展开更多
Nanopumps conducting fluids directionally through nanopores and nanochannels have attracted considerable interest for their potential applications in nanofiltration, water purification, and hydroelectric power generat...Nanopumps conducting fluids directionally through nanopores and nanochannels have attracted considerable interest for their potential applications in nanofiltration, water purification, and hydroelectric power generation Here, we demonstrate by molecular dynamics simulations that an excited vibrating carbon nanotube （CNT） cantilever can act as an efficient and simple nanopump. Water molecules inside the vibrating cantilever are driven by centrifugal forces and can undergo a continuous flow from the fixed to free ends of the CNT. Further extensive simulations show that the pumping function holds good not only for a single-file water chain in a narrow （6,6） CNT, but also for bulk-like water columns inside wider CNTs, and that the water flux increases monotonically with increasing diameter of the nanotube.展开更多
基金the National Natural Science Foundation of China(Grant No.60576047)
文摘A novel continuous-flow PCR chip adopting self-heating, passive-cooling mode to realize the DNA fragments amplification was presented. Using the ANSYS finite element analysis, the temperature distribution of the chip is simulated and analyzed.The optimal size of the chip is 30×22 mm2, the roundabout micro-channel is the 90 μm width, 40 μm depth. Two micro-heater with the nickel-chrome alloy material film are formed on the side of silicon belonging to denaturation and renaturation zones needed for PCR reaction, and two adiabatic structures with groove on side of silicon by anisotropy etching. By the mode of heating local zones at single side, three wider constant temperature zones could be formed, which are 60 ℃,72 ℃,95 ℃ and suitable for PCR,and the temperature-difference could be restricted in less than 5 ℃.
基金This work was supported by the 973 Program （No. 2007CB936204）, National and Jiangsu Province National Science Foundation （NSF） （Nos. 10732040, 10802037, 30970557, and BK2008042） of China, and Nanjing University of Aeronautics and Astronautics Funds （No. BCXJ08-02）. The authors thank Drs. Yitao Dai, Chun Tang, and Zhuhua Zhang for helpful discussions.
文摘Nanopumps conducting fluids directionally through nanopores and nanochannels have attracted considerable interest for their potential applications in nanofiltration, water purification, and hydroelectric power generation Here, we demonstrate by molecular dynamics simulations that an excited vibrating carbon nanotube （CNT） cantilever can act as an efficient and simple nanopump. Water molecules inside the vibrating cantilever are driven by centrifugal forces and can undergo a continuous flow from the fixed to free ends of the CNT. Further extensive simulations show that the pumping function holds good not only for a single-file water chain in a narrow （6,6） CNT, but also for bulk-like water columns inside wider CNTs, and that the water flux increases monotonically with increasing diameter of the nanotube.
