探讨连翘叶黄酮(flavonoids from Forsythia suspense leaves,FFL)对力竭运动小鼠的抗疲劳作用。36只ICR小鼠随机分为对照组、连翘叶黄酮小剂量组、连翘叶黄酮大剂量组,每组12只,给药组小鼠每日分别按1.5g生药/kg体重和3.0g生药/kg体重...探讨连翘叶黄酮(flavonoids from Forsythia suspense leaves,FFL)对力竭运动小鼠的抗疲劳作用。36只ICR小鼠随机分为对照组、连翘叶黄酮小剂量组、连翘叶黄酮大剂量组,每组12只,给药组小鼠每日分别按1.5g生药/kg体重和3.0g生药/kg体重剂量以连翘叶黄酮灌胃,对照组小鼠以生理盐水灌胃,连续20d后,将各组小鼠置于水中游泳至力竭,记录力竭运动时间,并测定各组小鼠的肌糖原、肝糖原、全血乳酸(LD)、血尿素氮(BUN)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和丙二醛(MDA)各项生化指标。连翘叶黄酮降低血乳酸和血清尿素氮含量,提高肝糖原、肌糖原的含量,降低MDA的含量,提高SOD、GSH-PX的活性。连翘叶黄酮具有一定的抗疲劳作用。展开更多
In addition to six known flavonoids quercitrin, hyperoside, avicularin, rutin, quercetin and kaemferol, a new flavonol glycoside named 6″_O_acetyl quercetin 3_O_β_ D _alloside (1) was isolated from the aerial par...In addition to six known flavonoids quercitrin, hyperoside, avicularin, rutin, quercetin and kaemferol, a new flavonol glycoside named 6″_O_acetyl quercetin 3_O_β_ D _alloside (1) was isolated from the aerial parts of Hypericum perforatum L. The structures were determined on the basis of spectroscopic methods (UV, IR, FAB_MS, 1H_NMR and 13 C NMR). Antifungal assay of all compounds showed that metabolite 1, quercitrin and quercetin were inhibitory to the growth of phytopathogenic fungus Helminthosporium sativum Pamel King et Bakke with minimum inhibitory concentrations (MICs) of 25, 50 and 50 μg/mL, respectively. Moreover, glycoside 1 and quercitrin were also shown to be able to inhibit the growth of Fusarium graminearum Schw. with MIC of 100 μg/mL. The MICs of ketoconazole used as control against the test fungi were 0.5 μg/mL in our assay.展开更多
Aim Isolation and structural elucidation of the constituents from the aerialpart of Hypericum perforatum L. Methods Column chromatography and TLC were used to isolate chemicalconstituents. Physico-chemical characteriz...Aim Isolation and structural elucidation of the constituents from the aerialpart of Hypericum perforatum L. Methods Column chromatography and TLC were used to isolate chemicalconstituents. Physico-chemical characterization and spectroscopic analysis were employed forstructural identification. Results Three flavonols were isolated and identified. Conclusion Compound1 is a novel natural product and its structure has been characterized to be 3, 5, 7-trihydroxy-3',4'-isopropyldioxy-flavone.展开更多
文摘探讨连翘叶黄酮(flavonoids from Forsythia suspense leaves,FFL)对力竭运动小鼠的抗疲劳作用。36只ICR小鼠随机分为对照组、连翘叶黄酮小剂量组、连翘叶黄酮大剂量组,每组12只,给药组小鼠每日分别按1.5g生药/kg体重和3.0g生药/kg体重剂量以连翘叶黄酮灌胃,对照组小鼠以生理盐水灌胃,连续20d后,将各组小鼠置于水中游泳至力竭,记录力竭运动时间,并测定各组小鼠的肌糖原、肝糖原、全血乳酸(LD)、血尿素氮(BUN)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和丙二醛(MDA)各项生化指标。连翘叶黄酮降低血乳酸和血清尿素氮含量,提高肝糖原、肌糖原的含量,降低MDA的含量,提高SOD、GSH-PX的活性。连翘叶黄酮具有一定的抗疲劳作用。
文摘In addition to six known flavonoids quercitrin, hyperoside, avicularin, rutin, quercetin and kaemferol, a new flavonol glycoside named 6″_O_acetyl quercetin 3_O_β_ D _alloside (1) was isolated from the aerial parts of Hypericum perforatum L. The structures were determined on the basis of spectroscopic methods (UV, IR, FAB_MS, 1H_NMR and 13 C NMR). Antifungal assay of all compounds showed that metabolite 1, quercitrin and quercetin were inhibitory to the growth of phytopathogenic fungus Helminthosporium sativum Pamel King et Bakke with minimum inhibitory concentrations (MICs) of 25, 50 and 50 μg/mL, respectively. Moreover, glycoside 1 and quercitrin were also shown to be able to inhibit the growth of Fusarium graminearum Schw. with MIC of 100 μg/mL. The MICs of ketoconazole used as control against the test fungi were 0.5 μg/mL in our assay.
文摘Aim Isolation and structural elucidation of the constituents from the aerialpart of Hypericum perforatum L. Methods Column chromatography and TLC were used to isolate chemicalconstituents. Physico-chemical characterization and spectroscopic analysis were employed forstructural identification. Results Three flavonols were isolated and identified. Conclusion Compound1 is a novel natural product and its structure has been characterized to be 3, 5, 7-trihydroxy-3',4'-isopropyldioxy-flavone.
