[Objective] Simple and reliable identification criteria used in screening phosphorus efficiency of soybean at seedling stage was discussed.[Method] Using the high and low phosphorus soil pot experiment,the soybean see...[Objective] Simple and reliable identification criteria used in screening phosphorus efficiency of soybean at seedling stage was discussed.[Method] Using the high and low phosphorus soil pot experiment,the soybean seedling stage＇s 5 criteria including RPH,RAW,RRW,RAPC and RRPC were studied.[Result] Low phosphorus stress had the low effects on relative plant height,the coefficient of variation was only 9.07%,has not achieved the remarkable level with its its criteria＇s relevance;Low phosphorus stress had the higher effects on relatively above-ground dry weight and relative dry weight of root,relatively above-ground phosphorus concentration and relative root phosphorus concentration,its coefficient of variation was also big,in turn is 26.67%,22.68%,24.01% and 15.87%,The correlation coefficient among the indicators had significantly or extremely significantly positive correlation.[Conclusion] Relatively above-ground dry weight and relative dry weight of root,relatively above-ground phosphorus concentration can be used as an important indicator of comprehensive evaluation of screening phosphorus efficiency of soybean at seedling stage,while the relative root phosphorus concentration can be used as secondary screening index.展开更多
cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones,...cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.展开更多
基金Supported by Foundation of Heilongjiang Provincial Department of Education"Biological Basis of the Roots from Different Soybean Genotypes Adapting to Low-phosphorus Soil"(10551033)Scientific Research Startup Foundation for Doctor Program of Mudanjiang Normal University(MSB200912)~~
文摘[Objective] Simple and reliable identification criteria used in screening phosphorus efficiency of soybean at seedling stage was discussed.[Method] Using the high and low phosphorus soil pot experiment,the soybean seedling stage＇s 5 criteria including RPH,RAW,RRW,RAPC and RRPC were studied.[Result] Low phosphorus stress had the low effects on relative plant height,the coefficient of variation was only 9.07%,has not achieved the remarkable level with its its criteria＇s relevance;Low phosphorus stress had the higher effects on relatively above-ground dry weight and relative dry weight of root,relatively above-ground phosphorus concentration and relative root phosphorus concentration,its coefficient of variation was also big,in turn is 26.67%,22.68%,24.01% and 15.87%,The correlation coefficient among the indicators had significantly or extremely significantly positive correlation.[Conclusion] Relatively above-ground dry weight and relative dry weight of root,relatively above-ground phosphorus concentration can be used as an important indicator of comprehensive evaluation of screening phosphorus efficiency of soybean at seedling stage,while the relative root phosphorus concentration can be used as secondary screening index.
文摘cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.
