基于代谢组和转录组的银缕梅叶片退红机制初探

为了探究银缕梅〔Parrotia subaequalis(H.T.Chang)R.M.Hao et H.T.Wei〕叶片在春季由红转绿的物质基础和分子机制,采用代谢组和转录组联合分析对银缕梅幼叶和成熟叶中差异花色苷类成分含量及差异表达基因进行比较分析,并对花色苷合成关键酶基因进行生物信息学分析。结果表明:幼叶中花色苷类成分的总相对含量约为成熟叶的27倍;幼叶中锦葵色素3-O-葡萄糖苷、锦葵色素3-O-半乳糖苷和芍药花素3-O-葡萄糖苷的相对含量明显高于其他花色苷类成分,且极显著(p<0.01)高于成熟叶中对应成分的相对含量。比较幼叶和成熟叶的转录组数据,共筛选出11000个差异表达基因。GO功能富集结果显示富集到催化活性的差异表达基因最多(3000个);KEGG富集分析结果显示差异表达基因显著富集在植物激素信号转导通路和苯丙烷生物合成通路。通过与葡萄(Vitis vinifera Linn.)中已知的花色苷合成关键酶氨基酸序列进行比对,共筛选出9个银缕梅花色苷合成酶基因,并且这9个基因在幼叶中的相对表达量明显高于成熟叶。qRT-PCR结果表明:PsCHS1、PsF3′H、Ps3GT和PsAOMT的相对表达量极显著高于成熟叶。PsCHS1、PsF3′H、Ps3GT和PsAOMT分别包含393、528、461和323个氨基酸残基;4个蛋白的二级结构均以α螺旋和无规卷曲为主,其中,PsF3′H不含β转角,其余3个蛋白均由α螺旋、β转角、延伸链和无规卷曲构成;4个蛋白的三级结构均含有相应蛋白家族的保守结构。系统进化树显示:PsCHS1与红花檵木(Loropetalum chinense var.rubrum Yieh)的LcCHS,PsF3′H与枫香树(Liquidambar formosana Hance)的LfF3′H,Ps3GT与可可(Theobroma cacao Linn.)的Tc3GT,以及PsAOMT与枫香树的LfAOMT分别首先聚在一起。综上所述,锦葵色素3-O-葡萄糖苷、锦葵色素3-O-半乳糖苷和芍药花素3-O-葡萄糖苷可能是银缕梅叶片退红的主效花色苷类成分,PsCHS1、PsF3′H、Ps3GT和PsAOMT可能是银缕梅叶片退红的关键基因。

退红舒缓中药面膜联合丹栀逍遥散治疗玫瑰痤疮患者的疗效及对其皮肤屏障功能、BDNF、TrKB表达的影响

目的探讨退红舒缓中药面膜联合丹栀逍遥散治疗肝郁脾虚型玫瑰痤疮患者的疗效及对其皮肤屏障功能、脑源性神经营养因子(Brain-derived neurotrophic factor,BDNF)、脑源性神经营养因子受体(Tropomyosin receptor kinase b,TrKB)表达的影响。方法选取2021年5月—2023年5月期间在湖南省中西医结合医院诊治的90例肝郁脾虚型玫瑰痤疮患者,采用随机数字表法分为对照组、中药组和联合治疗组,每组各30例。对照组患者外用退红舒缓中药面膜治疗,中药组患者口服丹栀逍遥散治疗,联合治疗组采用退红舒缓中药面膜联合丹栀逍遥散治疗,3组均持续治疗4周。观察比较3组患者临床疗效、不良反应情况,治疗前后中医证候积分、心理状态评分[汉密尔顿抑郁量表(Hamilton depression rating scale,HAMD)、汉密尔顿焦虑量表(Hamilton anxiety rating scale,HAMA)]、BDNF、TrKB蛋白和mRNA相对表达水平、炎症因子水平[白介素-4(Interleukin-4,IL-4)、白介素-17(Interleukin-17,IL-17)和白介素-37(Interleukin-37,IL-37)]。结果治疗后3组患者中医证候积分较治疗前降低,差异有统计学意义(P<0.05);且联合治疗组患者中医证候积分低于对照组和中药组,差异有统计学意义(P<0.05)。治疗后3组患者皮肤泛红、颜色评分和经表皮失水率较治疗前降低,角质层水平含量较治疗前升高,差异有统计学意义(P<0.05);且联合治疗组患者皮肤泛红、颜色评分和经表皮失水率低于对照组和中药组,角质层水平含量高于对照组和中药组,差异有统计学意义(P<0.05)。治疗后3组患者心理状态评分均较治疗前降低,差异有统计学意义(P<0.05);且联合治疗组患者心理状态评分低于对照组和中药组,差异有统计学意义(P<0.05)。治疗后3组患者BDNF、TrKB和BDNF mRNA、TrkB mRNA均较治疗前降低,差异有统计学意义(P<0.05),且联合治疗组患者BDNF、TrKB和BDNF mRNA、TrkB mRNA低于对照组和中药组,差异有统计学意义(P<0.05)。治疗后3组患者IL-4、IL-17和IL-37水平均较治疗前降低,差异有统计学意义(P<0.05);且联合治疗组患者IL-4、IL-17和IL-37水平低于对照组和中药组,差异有统计学意义(P<0.05)。治疗后联合治疗组临床愈显率为90.00%(27/30),较对照组63.33%(20/30)和中药组66.67%(19/30)明显升高,差异有统计学意义(P<0.05)。治疗期间,3组患者均未发生明显不良反应。结论退红舒缓中药面膜联合丹栀逍遥散治疗可以改善肝郁脾虚型玫瑰痤疮患者临床症状,提高皮肤屏障功能和心理水平,其机制可能与降低BDNF、TrkB表达有关。

以退红煎超声雾化为主治疗重症急性结膜炎86例

目的:观察以退红煎超声雾化为主,配合内服退红汤治疗重症急性结膜炎的临床疗效.方法:对136例经常规治疗7天以上无效的重症急性结膜炎患者,随机分为治疗组86例、对照组50例,进行治疗观察.结果:经3～14天治疗,136例全部治愈.治疗组治愈时间明显缩短,表明退红煎超声雾化配合内服退红汤治疗重症急性结膜炎,疗效满意.

活血美颗粒联合中药退红面膜治疗面部毛细血管扩张症疗效观察

目的:探讨活血美颗粒联合自制中药退红面膜治疗面部毛细血管扩张症的临床疗效。方法:收集面部单纯性毛细血管扩张症患者80例,随机分为两组。对照组:40例,外用他克莫司软膏,25mg/次,2次/d;治疗组:40例,服用活血美颗粒联合自制中药退红面膜。连续治疗7d为1个疗程。两组连续治疗4个疗程后观察临床症状及不良反应,利用VISIA软件判定疗效。结果:治疗4个疗程后,治疗组总有效率为92.5%,对照组为67.5%,治疗组疗效优于对照组,有显著性差异(P<0.05);治疗组患者临床症状得到明显改善,副作用小,而且停药后复发率较低。结论:活血美颗粒联合中药退红面膜治疗面部毛细血管扩张症疗效显著,可临床推广应用。

祛斑退红中药面膜治疗痤疮炎症后红斑及色素沉着的临床观察

目的:观察祛斑退红中药面膜治疗痤疮炎症后红斑及色素沉着的临床疗效和安全性。方法:采用随机、开放、同期对照的方法,将72例受试者随机分为试验组和对照组,每组36例。试验组连续使用4周中药面膜,每天1次,对照组4周内不使用中药面膜,为空白对照,试验期间,两组受试者均被告知做好面部基础护理,接受同样的痤疮护理宣教,并于治疗前和治疗后2、4周进行访视,评价皮损症状评分、视觉模拟评分(VAS)、VISIA皮肤检测仪分值、图像靶皮损与周围正常靶皮肤RGB均值差、临床疗效及安全性。结果:试验组红斑、色素沉着皮损症状评分均随着治疗时间的推进逐渐下降,对照组症状评分不受治疗时间的影响,且试验组红斑评分在治疗后4周显著低于对照组(P<0.05),色素沉着评分在治疗后2、4周均显著低于对照组(P<0.05)。试验组红斑、色素沉着、皮肤暗沉、毛孔粗大程度的VAS评分均随着治疗时间的推进逐渐下降,对照组VAS评分各项水平不受治疗时间的影响,且试验组VAS评分各项水平在治疗后2、4周均显著低于对照组(P<0.05)。试验组治疗后4周斑点、红色区、棕色区VISIA检测分值均低于治疗前,且红色区、棕色区分值均低于对照组。两组红斑、色素沉着靶皮损与正常靶皮肤RGB均值差均随着治疗时间的推进逐渐缩小,但试验组红斑、色素沉着的RGB均值差在治疗后2、4周均显著低于对照组(P<0.05)。两组红斑总有效率均随着治疗时间的推进逐渐增加,治疗后4周,试验组总有效率为85.29%(29/34),对照组为14.71%(5/34),试验组红斑总有效率显著高于对照组(P<0.01)。试验组色素沉着总有效率为76.47%(26/34),对照组为11.76%(4/34),试验组色素沉着总有效率显著高于对照组(P<0.01)。试验组不良反应发生3例,对照组不良反应发生0例,两组不良反应发生例数差异无统计学意义。结论:祛斑退红中药面膜治疗痤疮炎症后红斑及色素沉着疗效确切且安全性较高。

合肥市翡翠路精品化改造方案设计探析

翡翠路是合肥市连接政务、经开及肥西的重要商业、景观主轴,项目北起312国道,南至方兴大道,全长约7km,红线宽60m,双向八车道。文章主要针对功能提升与品质完善进行方案探讨,包括总体设计、交通改善、退红空间设计、绿化提升、城市家具设计等。

Carbon Sink Effects in Conversion of Farmland to Forest Project in Karst Drainage Basin——A Case of Hongfenghu Drainage Basin in Guizhou Province

[Objective]Study on carbon sink effects in Conversion of Farmland to Forest Project in Hongfenghu drainage basin in order to provide evidences for assessing carbon sink potential of conversion of farmland to forest in Guizhou Province.[Method]By investigating the implement of Conversion of Farmland to Forest Project in Hongfenghu drainage basin from 2000 to 2006,the carbon sink amount and effect of seven main tree species in the foreat region like Cunninghamia lanceolata,Cryptomeria fortunei,Amygdalus persica,Prunus salicina,Armeniaca vulgaris,Camptotheca acuminate and Catalpa bungei were calculated,based on which the amount of forest carbon sinks in Hongfenghu drainage basin in 2015 was estimated.[Result]Biomass storage and carbon sink amount in middle and young aged forests were increasing over time from 2000 to 2006,which reached 1.05×107 kg by 2006 and would engage more and more potential.Cunninghamia lanceolata has the superior carbon sink capacity in the seven tree species in the research region,of which the amount of carbon sink per unit area will be 106.51 t/hm2 by 2015,followed by Cryptomeria fortunei with the amount of carbon sink per unit area by 99.42 t/hm2.Armeniaca vulgaris has the weakest carbon sink capacity of all the seven species with the amount of 13.03 t/hm2.The total amount of carbon sink in seven tree species was 2.35×107 kg,while the average amount of carbon sink per unit area was 26.17 t/hm2,which could produced economic benefit of 7.17×106 yuan calculated on the price of 305.0 yuan/t or 5.91×106 yuan calculated on the price of 254.1 yuan/t.[Conclusion]Economic benefits of carbon sink effects of Conversion of Farmland to Forest Project in Hongfenghu drainage basin were great with huge appreciation potential.

Degradation of Soil Nutrients in Southeast China

A total of 2 190 soil nutrient data in the Second National Soil Survey of China were collected to assess thedegradation of soil nutrients in the hilly region of Southeast China. The definition of soil nutrient degradationis suggested firstly then the evaluation criteria are set up and the current status of degradation of red soil and latosol is assessed. The percentages of areas in four grades of soil nutrient degradation, i.e., slightlydeficient, medium deficient, severely deficient and extremely deficient, were 21.3%, 43.3%, 16.2% and 3.0%for soil total N; 0.7%, 6.4%, 16.7% and 76.2% for soil available P; and 25.4%, 26.3%, 8.6% and 5.0% forsoil available K, respectively. The severity of soil nutrient degradation was in the order of P > N > K. Themajor factors leading to the degradation of soil nutrients in quantity include soil erosion, leaching and theconsumption by crops. And the principal faCtor affecting the degradation of soil nutrieots in availability isthe fixation of N, P and K, especially the fixation of phosphorus. The average amouat of P fixed by soils is408 mg kg-1, and upland soils can fix more P than paddy soils.

Snstainable Land Use in Hilly Red Soil Region of South-eastern China

In the Present work, an integrated analysis of natural and socio-economic conditions in the hilly redsoil region of southeastern China is made by nieans of a Geographic Information System. Particular em-phasis lies on soil degradation and other adverse ecological and environmental effects of the prevalent andirrational land-use systems of mono-and valley-agriculture in the region. Moreover, taking into account thesimultaneous existence of enormous potentials and serious constraints and the high diversity and complexityof the biophysical and socio-economic environments, a set of strategies and counterineasures are also prc-posed for appropriate management of environmental resources, in terms of maintaining harmony betweenutilization and conservation of the natural environment, including 1) adjustment of the agricultural structureand optimization of ecological patterns of stereo-agriculture; 2) improvement and integrated exploitation ofthe low-yield lands and wastelands; 3) rehabilitation and conservation of soil and water resources; and 4)establishment of production bases of tropical and subtropical cash crops and fruits.

Eosinophilic cholangitis is a potentially underdiagnosed etiology in indeterminate biliary stricture

To investigate presence and extent of eosinophilic cholangitis (EC) as well as IgG4-related disease in patients with indeterminate biliary stricture (IBS).METHODSAll patients with diagnosis of sclerosing cholangitis (SC) and histopathological samples such as biopsies or surgical specimens at University Hospital Frankfurt from 2005-2015 were included. Histopathological diagnoses as well as further clinical course were reviewed. Tissue samples of patients without definite diagnosis after complete diagnostic work-up were reviewed regarding presence of eosinophilic infiltration and IgG4 positive plasma cells. Eosinophilic infiltration was as well assessed in a control group of liver transplant donors and patients with primary sclerosing cholangitis.RESULTSone hundred and thirty-five patients with SC were included. In 10/135 (13.5%) patients, no potential cause of IBS could be identified after complete diagnostic work-up and further clinical course. After histopathological review, a post-hoc diagnosis of EC was established in three patients resulting in a prevalence of 2.2% (3/135) of all patients with SC as well as 30% (3/10) of patients, where no cause of IBS was identified. 2/3 patients with post-hoc diagnosis of EC underwent surgical resection with suspicion for malignancy. Diagnosis of IgG4-related cholangitis was observed in 7/135 patients (5.1%), whereas 3 cases were discovered in post-hoc analysis. 6/7 cases with IgG4-related cholangitis (85.7%) presented with eosinophilic infiltration in addition to IgG4 positive plasma cells. There was no patient with eosinophilic infiltration in the control group of liver transplant donors (n = 27) and patients with primary sclerosing cholangitis (n = 14).CONCLUSIONEC is an underdiagnosed benign etiology of SC and IBS, which has to be considered in differential diagnosis of IBS.

TRHH-herb pair prevents IL-1β-induced degeneration of endplate chondrocytes in vitro

The inflammatory cytokine interleukin-1 beta （IL-1β） plays a key role in the process of intervertebral disc degenera- tion （IVDD）. In the present study, we aimed to evaluate the effect of pharmaco-serum of ＂Taoren-Honghua-herb pair＂ on IL-1β- induced chondrocyte degeneration in vitro. Taoren （Semen persicae） and Honghua （Safflower carthamus） were administered to the rats, and the pharmaco-serum was collected and prepared. Chondrocytes of the third passage, isolated from the rat＇s vertebral endplates, were treated by standard medium only （Group NC）, IL-1β （Group IL） or combination of IL-1β and pharmaco-serum （Group TRHH）. Cell proliferation and apoptosis were determined, and the expression of aggrecan, Col2ul, Coll0ul, IL-6 and SOX9 at the mRNA level in chondrocytes was quantified by real-time PCR. Immunohistochemistry staining of type II and X collagen and Safranine O staining were also used to evaluate the chondrocytes. Compared with the Group NC, IL-1β treatment inhibited the cell proliferation and induced the cell apoptosis （P〈0.05）, and the expression of aggrecan, Col2αl and SOX9 at the mRNA level was down-regulated. In contrast, the expression of Coll0ul and IL-6 was up-regulated after IL-1β treatment （P〈0.05）. Meanwhile, the immune-staining of type II collagen and Safranine O staining were decreased, while the staining of type X collagen was increased. Compared with the Group IL, cell proliferation was increased, and apoptosis of chondrocytes was decreased when cells were treated with the pharmaco-semm of TRHH-herb pair （P〈0.05）. The expression of aggrecan, Col2cd and SOX9 at the mRNA level was up-regulated, while that of Coll0cd and IL-6 was down-regulated （P〈0.05）. Saffanine O staining also showed increased positive staining （P〈0.05）. Taken together, the treatment of pharmaco-serum of TRHH-herb pair could prevent endplate chondrocyte degeneration induced by IL-1β.

Investigation of structural and magnetic properties of Ni implanted rutile

In this paper,the structural and magnetic properties of Ni metal implanted TiO 2 single crystals are discussed.Ni nanocrystals (NCs) have been formed in TiO 2 after ion implantation.Their crystalline sizes were increased with increasing post-annealing temperature.Metallic Ni NCs inside the TiO 2 matrix are stable up to an annealing temperature of 1073 K.The Ni NCs forming inside TiO 2 are the major contribution of the measured ferromagnetism.