The oil film clutch, which is superior in speed adjustment, is capable of being used in the belt conveyor to carry out soft start operation. According to running properties of both belt conveyor and oil film clutch...The oil film clutch, which is superior in speed adjustment, is capable of being used in the belt conveyor to carry out soft start operation. According to running properties of both belt conveyor and oil film clutch, equations that are used to decide the lubricating oil flow and the number of oil films (i.e. the number of rotating and stationary plates) are deduced theoretically. Also key parameters are provided for the design of the hydraulic system. All these together provide the theoretical basis for the soft start design of the belt conveyor and references for the application of the oil film clutch in similar fields.展开更多
Air film conveyors equipped with porous pads have been developed to bring the liquid crystal display(LCD) into a non-contact state during transportation process. In this work, a theoretical model including flow proper...Air film conveyors equipped with porous pads have been developed to bring the liquid crystal display(LCD) into a non-contact state during transportation process. In this work, a theoretical model including flow property of porous media and Reynolds equation is established within a representative region in order to optimize the design parameters of a partial porous air conveyor. With the theoretical model, an optimization method using nondominated sorting genetic algorithm – II(NSGA-II) is applied for a two-objective optimization to achieve a minimum air consumption and maximum load capacity. Three Pareto-optimal solutions are selected to analyze the influence of each parameter on the characteristics of the air conveyor, and the results indicate that the position of the porous pads has the most significant impact on the performance and of course must be determined with care. Furthermore, experimental results in terms of the supporting force versus gap clearance show that the optimized air conveyor can greatly improve the load capacity over the normal one, indicating that the optimization method is applicable for practical use.展开更多
In this paper, the air film formation behavior of air cushion belt conveyor with stable load is studied. The air cushion field is analyzed by means of theoretical derivation, numerical simulation and experimental rese...In this paper, the air film formation behavior of air cushion belt conveyor with stable load is studied. The air cushion field is analyzed by means of theoretical derivation, numerical simulation and experimental research. An intelligent experiment platform is developed. Three dimensional pressure distribution of air film and the air film thickness distribution along the conveyor belt in the width direction are obtained. The experimental result is analyzed by comparing with theoretical calculation and numerical simulation. The numerical and theoretical results are in good agreement with those obtained from experiments. The air film formation behavior pattern of air cushion belt conveyor with stable load is presented. The optimized film thickness and pore distribution are obtained based on the comprehensive energy consumption. This study provides a basis for the optimization design of air cushion belt conveyor.展开更多
The P2X3 receptor plays a vital role in sensory processing and transmission. The assembly and trafficking of the P2X3 receptor are important for its function in primary sensory neurons. As an important inflammation me...The P2X3 receptor plays a vital role in sensory processing and transmission. The assembly and trafficking of the P2X3 receptor are important for its function in primary sensory neurons. As an important inflammation mediator, ATP is released from different cell types around primary sensory neurons, especially under pathological pain conditions. Here, we showthat α, β-MeATP dramatically promoted membrane delivery of the P2X3 receptor both in HEK293T celts expressing recombinant P2X3 receptor and in rat primary sensory neurons. α, β-MeATP induced P2X3 receptor-mediated Ca^2＋ influx, which further activated Ca^2＋/calmodulin-dependent protein kinase Ilec （CaMKIIα）. The N terminus of the P2X3 receptor was responsible for CaMKIleα binding, whereas Thr38s in the C terminus was phosphorylated by CaMKIIα. Thr^388 phosphorylation increased P2X3 receptor binding to caveoUn-1. CaveoUn-1 knockdown abrogated the α, β-MeATP-induced membrane insertion of the P2X3 receptor. Moreover,α, β-MeATP drove the CaMKIlec-mediated membrane coinsertion of the P2X2 receptor with the P2X3 receptor. The increased P2X3 receptors on the cell membrane that are due to Thr388 phosphorytation facilitated P2X3 receptor-mediated signal transduction. Together, our data indicate that CaMKIIoL and caveoUn-1 cooperate to drive Ugand-induced membrane delivery of the P2X3 receptor and may provide a mechanism of P2X3 receptor sensitization in pain development.展开更多
Interests in intracellular peptide delivery have continued to grow,significantly fueled by the importance of peptides and their mimetics in modern cell biology and pharmaceutical industry.However,efficient intracellul...Interests in intracellular peptide delivery have continued to grow,significantly fueled by the importance of peptides and their mimetics in modern cell biology and pharmaceutical industry.However,efficient intracellular delivery of membrane-impermeable peptides of different polarities remains a challenging task.In this study,we develop a general and robust strategy for intracellular peptide delivery by using a boronic acid-rich dendrimer.The designed material is capable of transporting peptides with different polarities and charge properties into the cytosol of various cell lines without inducing additional cytotoxicity.The transduction efficacy and proteolytic stability of cargo peptides delivered by the boronic acid-rich dendrimer are much superior to peptides conjugated with cell penetrant peptides such as octaarginine.In addition,the bioactivities of pro-apoptotic peptides are maintained after intracellular delivery.This study provides a versatile and robust platform for the intracellular delivery of membrane-impermeable peptides.展开更多
文摘The oil film clutch, which is superior in speed adjustment, is capable of being used in the belt conveyor to carry out soft start operation. According to running properties of both belt conveyor and oil film clutch, equations that are used to decide the lubricating oil flow and the number of oil films (i.e. the number of rotating and stationary plates) are deduced theoretically. Also key parameters are provided for the design of the hydraulic system. All these together provide the theoretical basis for the soft start design of the belt conveyor and references for the application of the oil film clutch in similar fields.
基金Project(51205174)supported by the National Natural Science Foundation of ChinaProject(2014M550309)supported by the Postdoctoral Science Foundation of ChinaProject(GZKF-201407)supported by the Open Foundation of the State Key Laboratory of Fluid Power Transmission and Control,China
文摘Air film conveyors equipped with porous pads have been developed to bring the liquid crystal display(LCD) into a non-contact state during transportation process. In this work, a theoretical model including flow property of porous media and Reynolds equation is established within a representative region in order to optimize the design parameters of a partial porous air conveyor. With the theoretical model, an optimization method using nondominated sorting genetic algorithm – II(NSGA-II) is applied for a two-objective optimization to achieve a minimum air consumption and maximum load capacity. Three Pareto-optimal solutions are selected to analyze the influence of each parameter on the characteristics of the air conveyor, and the results indicate that the position of the porous pads has the most significant impact on the performance and of course must be determined with care. Furthermore, experimental results in terms of the supporting force versus gap clearance show that the optimized air conveyor can greatly improve the load capacity over the normal one, indicating that the optimization method is applicable for practical use.
基金partially supported by the National Natural Science Foundation of China (Grant Nos. 51075025 and 51175029)the Beijing Natural Science Foundation (Grant No. 3123041)
文摘In this paper, the air film formation behavior of air cushion belt conveyor with stable load is studied. The air cushion field is analyzed by means of theoretical derivation, numerical simulation and experimental research. An intelligent experiment platform is developed. Three dimensional pressure distribution of air film and the air film thickness distribution along the conveyor belt in the width direction are obtained. The experimental result is analyzed by comparing with theoretical calculation and numerical simulation. The numerical and theoretical results are in good agreement with those obtained from experiments. The air film formation behavior pattern of air cushion belt conveyor with stable load is presented. The optimized film thickness and pore distribution are obtained based on the comprehensive energy consumption. This study provides a basis for the optimization design of air cushion belt conveyor.
文摘The P2X3 receptor plays a vital role in sensory processing and transmission. The assembly and trafficking of the P2X3 receptor are important for its function in primary sensory neurons. As an important inflammation mediator, ATP is released from different cell types around primary sensory neurons, especially under pathological pain conditions. Here, we showthat α, β-MeATP dramatically promoted membrane delivery of the P2X3 receptor both in HEK293T celts expressing recombinant P2X3 receptor and in rat primary sensory neurons. α, β-MeATP induced P2X3 receptor-mediated Ca^2＋ influx, which further activated Ca^2＋/calmodulin-dependent protein kinase Ilec （CaMKIIα）. The N terminus of the P2X3 receptor was responsible for CaMKIleα binding, whereas Thr38s in the C terminus was phosphorylated by CaMKIIα. Thr^388 phosphorylation increased P2X3 receptor binding to caveoUn-1. CaveoUn-1 knockdown abrogated the α, β-MeATP-induced membrane insertion of the P2X3 receptor. Moreover,α, β-MeATP drove the CaMKIlec-mediated membrane coinsertion of the P2X2 receptor with the P2X3 receptor. The increased P2X3 receptors on the cell membrane that are due to Thr388 phosphorytation facilitated P2X3 receptor-mediated signal transduction. Together, our data indicate that CaMKIIoL and caveoUn-1 cooperate to drive Ugand-induced membrane delivery of the P2X3 receptor and may provide a mechanism of P2X3 receptor sensitization in pain development.
基金supported by the National Natural Science Foundation of China (21725402)the Science and Technology Commission of Shanghai Municipality (17XD1401600)+1 种基金Guangdong Innovative and Entrepreneurial Research Team Program (2016ZT06C322)supports from the Flow Cytometry Core Facility and the Confocal Microscopy Facility at ECNU
文摘Interests in intracellular peptide delivery have continued to grow,significantly fueled by the importance of peptides and their mimetics in modern cell biology and pharmaceutical industry.However,efficient intracellular delivery of membrane-impermeable peptides of different polarities remains a challenging task.In this study,we develop a general and robust strategy for intracellular peptide delivery by using a boronic acid-rich dendrimer.The designed material is capable of transporting peptides with different polarities and charge properties into the cytosol of various cell lines without inducing additional cytotoxicity.The transduction efficacy and proteolytic stability of cargo peptides delivered by the boronic acid-rich dendrimer are much superior to peptides conjugated with cell penetrant peptides such as octaarginine.In addition,the bioactivities of pro-apoptotic peptides are maintained after intracellular delivery.This study provides a versatile and robust platform for the intracellular delivery of membrane-impermeable peptides.
