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核糖开关的结构和调控机理 被引量:3
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作者 王少伟 李锡香 《生物技术通报》 CAS CSCD 北大核心 2010年第5期16-22,33,共8页
一种新发现的RNA分子——核糖开关,通过感知代谢物浓度的变化调控目标基因的表达。它可以调整自身的结构直接结合代谢物小分子,而不需要蛋白因子的参与。在原核生物中发现了大量的核糖开关,在真核生物如植物和真菌中也发现了核糖开关。... 一种新发现的RNA分子——核糖开关,通过感知代谢物浓度的变化调控目标基因的表达。它可以调整自身的结构直接结合代谢物小分子,而不需要蛋白因子的参与。在原核生物中发现了大量的核糖开关,在真核生物如植物和真菌中也发现了核糖开关。核糖开关由适体域和表达平台两个功能域组成,能在不同水平调控基因的表达,如转录终止、翻译起始、mRNA剪辑和加工。核糖开关不需要蛋白因子的参与,因此人们认为它可能是古代RNA世界的遗留物。核糖开关作为RNA传感器可以设计成一种基因控制元件,在未来的基因治疗方面可能具有很大的应用前景。 展开更多
关键词 核糖开关 适体域 表达平台 基因表达调控
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Experimental study on applicability of using time-domain reflectometry to detect NAPLs contaminated sands 被引量:5
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作者 ZHAN LiangTong MU QingYi +1 位作者 CHEN YunMin CHEN RenPeng 《Science China(Technological Sciences)》 SCIE EI CAS 2013年第6期1534-1543,共10页
Underground contamination by non-aqueous phase liquids (NAPLs) becomes increasingly serious. Rapid and reliable detection of contaminated zone and degree is the first step to site remediation. In this paper, diesel an... Underground contamination by non-aqueous phase liquids (NAPLs) becomes increasingly serious. Rapid and reliable detection of contaminated zone and degree is the first step to site remediation. In this paper, diesel and fine sand are used as experiment materials to investigate the applicability of using time-domain reflectometry (TDR) to detect LNAPLs contamination. The major work includes: measurement of dielectric constant and electrical conductivity for the diesel-water-air-sand mixtures; measurement of reflection waveform and dielectric constant for specimens with a diesel contaminated layer being sandwiched in sand. The experimental results show the followings: A significant decrease in both dielectric constant and electrical conductivity is observed for the diesel-water-air-sand mixtures when diesel displaces the pore water, and the content of diesel can be calculated by the model; insignificant change in dielectric properties is measured when diesel only displaces the pore gas; when the diesel contaminated sand is sandwiched between two saturated sand layers, the interfaces of the diesel contaminated layer can be identified by analyzing the reflection waveform; for field application, TDR method is valid for the case that LNAPLs seep into saturated sand layer, and the applicability of TDR method in vadose zone depends on the initial saturation of the sand layer. The findings obtained in this paper provide a guidance for the use of TDR for the field investigation of NAPLs contaminated site. 展开更多
关键词 LNAPLS SAND time domain reflectometry dielectric constant electrical conductivity
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AN ADAPTIVE TRUST REGION METHOD FOR EQUALITY CONSTRAINED OPTIMIZATION 被引量:1
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作者 ZHANGJuliang ZHANGXiangstm ZHUOXinjian 《Journal of Systems Science & Complexity》 SCIE EI CSCD 2003年第4期494-505,共12页
In this paper, a trust region method for equality constrained optimizationbased on nondifferentiable exact penalty is proposed. In this algorithm, the trail step ischaracterized by computation of its normal component ... In this paper, a trust region method for equality constrained optimizationbased on nondifferentiable exact penalty is proposed. In this algorithm, the trail step ischaracterized by computation of its normal component being separated from computation of itstangential component, i.e., only the tangential component of the trail step is constrained by trustradius while the normal component and trail step itself have no constraints. The other maincharacteristic of the algorithm is the decision of trust region radius. Here, the decision of trustregion radius uses the information of the gradient of objective function and reduced Hessian.However, Maratos effect will occur when we use the nondifferentiable exact penalty function as themerit function. In order to obtain the superlinear convergence of the algorithm, we use the twiceorder correction technique. Because of the speciality of the adaptive trust region method, we usetwice order correction when p = 0 (the definition is as in Section 2) and this is different from thetraditional trust region methods for equality constrained optimization. So the computation of thealgorithm in this paper is reduced. What is more, we can prove that the algorithm is globally andsuperlinearly convergent. 展开更多
关键词 equality constrained optimization global convergence trust region method superlinear convergence nondifferentiable exact penalty function maratos effect
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Automatically building large-scale named entity recognition corpora from Chinese Wikipedia
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作者 Jie ZHOU Bi-cheng LI Gang CHEN 《Frontiers of Information Technology & Electronic Engineering》 SCIE EI CSCD 2015年第11期940-956,共17页
Named entity recognition (NER) is a core component in many natural language processing applications. Most NER systems rely on supervised machine learning methods, which depend on time-consuming and expensive annotat... Named entity recognition (NER) is a core component in many natural language processing applications. Most NER systems rely on supervised machine learning methods, which depend on time-consuming and expensive annotations in different languages and domains. This paper presents a method for automatically building silver-standard NER corpora from Chinese Wikipedia. We refine novel and language-dependent features by exploiting the text and structure of Chinese Wikipedia. To reduce tagging errors caused by entity classification, we design four types of heuristic rules based on the characteristics of Chinese Wikipedia and train a supervised NE classifier, and a combined method is used to improve the precision and coverage. Then, we realize type identification of implicit mention by using boundary information of outgoing links. By selecting the sentences related with the domains of test data, we can train better NER models. In the experiments, large-scale NER corpora containing 2.3 million sentences are built from Chinese Wikipedia. The results show the effectiveness of automatically annotated corpora, and the trained NER models achieve the best performance when combining our silver-standard corpora with gold-standard corpora. 展开更多
关键词 NER corpora Chinese Wikipedia Entity classification Domain adaptation Corpus selection
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