To investigate the adaptation of poliovirus Sabin 2 in human embryonic lung diploid fibroblast（KMB17） and its relation to nucleotide mutations of 5′ noncoding region（5′NCR）,the Sabin 2 vaccine virus was passaged...To investigate the adaptation of poliovirus Sabin 2 in human embryonic lung diploid fibroblast（KMB17） and its relation to nucleotide mutations of 5′ noncoding region（5′NCR）,the Sabin 2 vaccine virus was passaged serially in KMB17 for seventeen passages.The infectious titers of Sabin 2 virus increased along with the passages,the highest titer reached 8.0 LgCCID50/ml at passage 13（P13）.During passage,there were three nucleotide mutations appeared in 5′NCR,G changed to C at position 20,C to U at position 189 and G to A at position 481.The results showed that Sabin 2 poliovirus adapted well in KMB17 after 17 passages.The nucleotide mutation at position 481 that appeared from passage 3 on indicated the possibility of virulent reversion.展开更多
In this paper, we propose two novel efficient scheduling schemes with network coding in multi-relay wireless network to maximize the transmission efficiency. The first one uses adaptive forwarding with network coding(...In this paper, we propose two novel efficient scheduling schemes with network coding in multi-relay wireless network to maximize the transmission efficiency. The first one uses adaptive forwarding with network coding(AF-NC), in which each relay adaptively calculates the number of packets having innovative information according to the feedback from the sink. With AF-NC, duplicate packets are not sent, and the total number of time slots needed to complete transmission can be significantly reduced. The second scheme, named adaptive forwarding with network coding and retransmission(AFR-NC), combines AF-NC with automatic repeat request(ARQ) to guarantee reliable end-to-end communication with limited resource occupation. Numerical results show that compared with simple forwarding with network coding(F-NC), AF-NC has close successful delivery rate with dramatically less time slots, while AFR-NC achieves strict reliability with limited resource cost.展开更多
文摘To investigate the adaptation of poliovirus Sabin 2 in human embryonic lung diploid fibroblast（KMB17） and its relation to nucleotide mutations of 5′ noncoding region（5′NCR）,the Sabin 2 vaccine virus was passaged serially in KMB17 for seventeen passages.The infectious titers of Sabin 2 virus increased along with the passages,the highest titer reached 8.0 LgCCID50/ml at passage 13（P13）.During passage,there were three nucleotide mutations appeared in 5′NCR,G changed to C at position 20,C to U at position 189 and G to A at position 481.The results showed that Sabin 2 poliovirus adapted well in KMB17 after 17 passages.The nucleotide mutation at position 481 that appeared from passage 3 on indicated the possibility of virulent reversion.
基金the National Natural Science Foundation of China(Nos.61102051,61221001 and 61301117)the National High Technology Research and Development Program(863)of China(Nos.2012AA011701 and 2012AA121601)+1 种基金the Shanghai Jiao Tong University Science and Technology Innovation Foundation(No.AF0300021)the Shanghai Key Laboratory Funding(No.12DZ2272600)
文摘In this paper, we propose two novel efficient scheduling schemes with network coding in multi-relay wireless network to maximize the transmission efficiency. The first one uses adaptive forwarding with network coding(AF-NC), in which each relay adaptively calculates the number of packets having innovative information according to the feedback from the sink. With AF-NC, duplicate packets are not sent, and the total number of time slots needed to complete transmission can be significantly reduced. The second scheme, named adaptive forwarding with network coding and retransmission(AFR-NC), combines AF-NC with automatic repeat request(ARQ) to guarantee reliable end-to-end communication with limited resource occupation. Numerical results show that compared with simple forwarding with network coding(F-NC), AF-NC has close successful delivery rate with dramatically less time slots, while AFR-NC achieves strict reliability with limited resource cost.
