The paper aims to study agricultural land suitability in typical agricultural area in Huanghuaihai plain by the comprehensive evaluation of natural factors and environmental conditions of agricultural land with Liaoch...The paper aims to study agricultural land suitability in typical agricultural area in Huanghuaihai plain by the comprehensive evaluation of natural factors and environmental conditions of agricultural land with Liaocheng area in Shangdong Province in the center of Huanghuaihai plain as a case.The results suggested that the land quality in Liaocheng was above the average and accorded with the local practical situation.The authors validated the scientificity of agricultural land suitability study by using GIS technology and proposed some suggestions on analysis results.展开更多
[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant D...[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.展开更多
基金Support by Research of Comprehensive Evaluation of Agricultural Land Suitability in Typical Area of Huanghuahai Plain based on GIS(X0810025)~~
文摘The paper aims to study agricultural land suitability in typical agricultural area in Huanghuaihai plain by the comprehensive evaluation of natural factors and environmental conditions of agricultural land with Liaocheng area in Shangdong Province in the center of Huanghuaihai plain as a case.The results suggested that the land quality in Liaocheng was above the average and accorded with the local practical situation.The authors validated the scientificity of agricultural land suitability study by using GIS technology and proposed some suggestions on analysis results.
基金Supported by Major Projects of Cultivating New Varieties by Trans-genic Technology (2008ZX08012-001)~~
文摘[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.
