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和胃化痰降逆治反流性食管炎 被引量:1
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作者 伏新顺 《家庭医学(上半月)》 2008年第8期24-24,共1页
反流性食管炎属中医“反胃”、“噎嗝”、“嘈杂”、“吞酸”、“食管瘅”等范畴。其病因是由于上消化道动力障碍引起的酸和威碱相关性疾病,以及食管下括约肌张力低下、食管清除能力减弱、胃排空延迟等,导致胃酸、胃蛋白酶及胃内容物... 反流性食管炎属中医“反胃”、“噎嗝”、“嘈杂”、“吞酸”、“食管瘅”等范畴。其病因是由于上消化道动力障碍引起的酸和威碱相关性疾病,以及食管下括约肌张力低下、食管清除能力减弱、胃排空延迟等,导致胃酸、胃蛋白酶及胃内容物由胃反流入食管;或者以胆汁为主的碱性十二指肠液自下而上反流入胃,和胃中的胃酸、胃蛋白酶及胃内容物一起向上反流入食管, 展开更多
关键词 反流性食管炎 和胃化痰 消化道动力障碍 逆治 胃内容物 胃蛋白酶 相关性疾病 肌张力低下
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《内经》“逆治”“从治”有三义 被引量:1
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作者 欧阳兵 孟令军 《成都中医学院学报》 1990年第4期43-44,共2页
对《内经》“逆治”与“从治”的涵义进行了较深入的发掘和系统的分析。认为其涵义有三:一指对病证的逆从,二指对气机的逆从,三指对标本的逆从。并指出,对于病证的逆从,已为学术界所公认并得到充分的重视。对于气机的逆从,标本的逆从,... 对《内经》“逆治”与“从治”的涵义进行了较深入的发掘和系统的分析。认为其涵义有三:一指对病证的逆从,二指对气机的逆从,三指对标本的逆从。并指出,对于病证的逆从,已为学术界所公认并得到充分的重视。对于气机的逆从,标本的逆从,则为学术界所忽略。同时,着重对后者进行了分析。 展开更多
关键词 黄帝内经 逆治
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加减当归四逆治汤疗手足冻疮106例 被引量:1
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作者 汪仁柏 《陕西中医学院学报》 1989年第1期56-56,共1页
1.方剂:加减当归四逆汤即当归30克,桂枝30克、细辛10克、红花10克、黄芪20克。 2.用法:将上药放入洗脚瓷盆或铝盆中,加水2000毫升左右,用火煎开后,再煎10分钟即可。然后待药液温度下降至35~42℃之间,将患肢浸泡药液中,感觉不凉不烫为... 1.方剂:加减当归四逆汤即当归30克,桂枝30克、细辛10克、红花10克、黄芪20克。 2.用法:将上药放入洗脚瓷盆或铝盆中,加水2000毫升左右,用火煎开后,再煎10分钟即可。然后待药液温度下降至35~42℃之间,将患肢浸泡药液中,感觉不凉不烫为宜。水温过高会增加局部组织代谢,造成细胞更多的损害;温度过低达不到复温和治疗效果。 展开更多
关键词 逆治 药液温度 组织代谢 复温 血栓性静脉炎 此方 黄茂
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顽固性呃逆治验
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作者 徐秀平 许红 《解放军医药杂志》 CAS 1997年第6期410-410,共1页
病例 男,16岁,学生。3个月前与人争吵后,出现呃逆伴头胀痛,陶胁胀闷。近1个月来,呃逆连声,头胀痛,严重至不能坚持上学,胸胁胀满,大便秘结,2~3日一次,舌红苔微黄而腻,脉弦滑。脉症合参,证属肝胃气滞,治以疏肝理气解郁,和胃降逆止呃之法。
关键词 顽固性呃 逆治 情志不畅 脉症合参 肝胃气滞 理气解郁 古今医统大全 胸胁胀满 大便秘结 旋复花
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逆治从治新释
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作者 罗志平 《国医论坛》 1999年第4期39-41,共3页
关键词 逆治
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国医大师李士懋论“观其脉证,知犯何逆,随证治之” 被引量:4
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作者 扈有芹 李玉昌 《环球中医药》 CAS 2016年第6期695-696,共2页
李士懋教授精研《伤寒论》,对《伤寒论》提出的"观其脉证,知犯何逆,随证治之"的观点领会深刻。认为"观其脉证,知犯何逆,随证治之"是对《内经》"谨守病机"的疾病治疗观的具体实践;是仲景提出的辨证论治总... 李士懋教授精研《伤寒论》,对《伤寒论》提出的"观其脉证,知犯何逆,随证治之"的观点领会深刻。认为"观其脉证,知犯何逆,随证治之"是对《内经》"谨守病机"的疾病治疗观的具体实践;是仲景提出的辨证论治总的指导原则;应用在疾病治疗过程的始终。 展开更多
关键词 李士懋 伤寒论 观其脉证 知犯何 随证
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温肾利水法治疗老人尿频
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作者 马献军 《家庭中医药》 2016年第4期48-49,共2页
诗曰:老人尿频扰心情,夜起小便八次醒。手术切除前列腺,问题未解尿频仍。中医辨证整体观,阳虚水阻需要通。温肾利水逆治法,攻补兼施通为用。阳火得助水通利,两觉睡到大天明。诗解:老人尿频是指老年人的小便次数多,每晚起夜少则三五次... 诗曰:老人尿频扰心情,夜起小便八次醒。手术切除前列腺,问题未解尿频仍。中医辨证整体观,阳虚水阻需要通。温肾利水逆治法,攻补兼施通为用。阳火得助水通利,两觉睡到大天明。诗解:老人尿频是指老年人的小便次数多,每晚起夜少则三五次,多则七八次,既干扰了老人的正常睡眠,又影响了老人的心情。 展开更多
关键词 温肾利水 前列腺增生肥大 阳火 西医检查 正常睡眠 攻补兼施 逆治 水阻 肾为先天之本 水液代谢
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胃食管反流病的中医治疗对策 被引量:9
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作者 吴卫莲 张存钧 《上海中医药杂志》 2009年第10期28-29,共2页
关键词 胃食管反流病 中医药疗法 从热论 从气论 从酸论
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Asthma and gastroesophageal reflux disease: Effect of long-term pantoprazole therapy 被引量:10
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作者 Calabrese Carlo Fabbri Anna +3 位作者 Areni Alessandra Scialpi Carlo Zahlane Desiree Di Febo Giulio 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第48期7657-7660,共4页
AIM: To define the prevalence of gastroesophageal reflux disease (GERD) in mild persistent asthma and to value the effect of pantoprazole therapy on asthmatic symptoms.METHODS: Seven of thirty-four asthmatic patie... AIM: To define the prevalence of gastroesophageal reflux disease (GERD) in mild persistent asthma and to value the effect of pantoprazole therapy on asthmatic symptoms.METHODS: Seven of thirty-four asthmatic patients without GERD served as the non-GERD control group. Twenty-seven of thirty-four asthmatic patients had GERD (7/27 also had erosive esophagitis, sixteen of them presented GERD symptoms. An upper gastrointestinal endoscopy was performed in all the subjects to obtain five biopsy specimens from the lower 5 cm of the esophagus. Patients were considered to have GERD when they had a dilation of intercellular space (DIS)〉0.74 μm at transmission electron microscopy. Patients with GERD were treated with pantoprazole, 80 mg/day. Forced expiratory volume in one second (FEV1) was performed at entry and after 6 mo of treatment. Asthmatic symptoms were recorded. The required frequency of inhaling rapid acting β2-agonists was self-recorded in the patients' diaries.RESULTS: Seven symptomatic patients presented erosive esophagitis. Among the 18 asymptomatic patients, 11 presented DIS, while all symptomatic patients showed ultrastructural esophageal damage. Seven asymptomatic patients did not present DIS. At entry the mean of FEV1 was 1.91 L in symptomatic GERD patients and 1.88 L in asymptomatic GERD patients. After the treatment, 25 patients had a complete recovery of DIS and reflux symptoms. Twenty-three patients presented a regression of asthmatic symptoms with normalization of FEV1. Four patients reported a significant improvement of symptoms and their FEV1 was over 80%.CONCLUSION: GERD is a highly prevalent condition in asthma patients. Treatment with pantoprazole (80 mg/day) determines their improvement and complete regression. 展开更多
关键词 ASTHMA Gastroesophageal reflux disease PANTOPRAZOLE NERD ERD Dilated intercellular spaces TEN
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Effect of itopride, a new prokinetic, in patients with mild GERD: A pilot study 被引量:15
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作者 Yong Sung Kim Tae Hyeon Kim +6 位作者 Chang Soo Choi Young Woo Shon Sang Wook Kim Geom Seog Seo Yong Ho Nah Myung Gyu Choi Suck Chei Choi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第27期4210-4214,共5页
AIM: Itopride is a newly developed prokinetic agent, which enhances gastric motility through both antidopaminergic and anti-acetylcholinesterasic actions. The importance of esophageal motor dysfunction in the pathoge... AIM: Itopride is a newly developed prokinetic agent, which enhances gastric motility through both antidopaminergic and anti-acetylcholinesterasic actions. The importance of esophageal motor dysfunction in the pathogenesis of gastro-esophageal reflux disease (GERD) makes it interesting to examine the effect of itopride on esophageal acid exposure.METHODS: The effect of itopride on esophageal acid reflux variables for 24 h was studied in 26 patients with GERD symptoms, pre-entry total acid exposure time (pH〈4) of more than 5% and mild esophagitis (SavaryMiller grades I, II) proven by endoscopy. Ambulatory 24hpH-metry and symptom assessment were performed after treatments with 150 or 300 mg itopride thrice a day (t.i.d.) for 30 d in random order, using an open label method.For evaluating the safety of itopride, blood biochemical laboratory test was performed and the serum prolactin level was also examined before and after treatment.RESULTS: Total symptom score was significantly decreased after treatment in 150- or 300-mg group. Itopride 300 mg was significantly effective than 150 mg on decreasing the total per cent time with pH〈4, total time with pH〈4 and DeMeester score. No serious adverse effects were observed with administration of itopride in both groups.CONCLUSION: Itopride 100 mg t.i.d, is effective on decreasing pathologic reflux in patient with GERD and therefore it has the potential to be effective in the treatment of this disease. 展开更多
关键词 Gastro-esophageal reflux disease ITOPRIDE
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Experimental Therapy for Solid Tumor by in situ Retroviral-mediated Gene Transfer
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作者 周中军 牛大地 +1 位作者 汤健 周爱儒 《Developmental and Reproductive Biology》 1995年第2期39-45,共7页
Although chemotherapy plays a main role in treatment for cancer, gene therapy is thought to be a promising approach of treatment for the future. Thymidine-Kinase gene was delivered into proliferating cells by a retrov... Although chemotherapy plays a main role in treatment for cancer, gene therapy is thought to be a promising approach of treatment for the future. Thymidine-Kinase gene was delivered into proliferating cells by a retroviral mediated gene transfer system in the culture cells. Later treatment with GCV showed definite cytotoxic effect on the HSV-tk modified cells and the effect correlated with the growth rate of cells. The rapid proliferating tumor cells, HR8348, were inhibited more efficiently than slow proliferating cells. In experiment in vivo , the introduced TK producer cells released virus particles continuously into their neighbor and, as compared with the control, GCV treatment exerted remarkable inhibitory effect on the growth of tumor. The inhibition also correlated with the number of injected TK producer cells. With administration of 0.2 ml of #795 tumor cell suspension(100 ng/ml), coinjection of 2 ×106 TK producer cells achieved 37% inhibition while that of 1 ×107 TK producer cells achieved 66% inhibition,though complete regression could not be achieved under such circumstances.Our result suggests that TK gene transfer followed by GCV treatment merits further evaluation as an effective antineoplastic approach. 展开更多
关键词 HSV-TK Gene therapy TUMOR
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Reversible cardiogenic shock caused by atrioventricular junctional rhythm after percutaneous coronary intervention 被引量:1
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作者 Jun Wang Qi-Gao Zhang +3 位作者 Xiao-Min Cai Li-Jun Wang Jian-Bin Gong Shi-Sen Jiang 《Journal of Geriatric Cardiology》 CAS CSCD 2012年第3期318-320,共3页
An 82-year-old female patient undergoing cardiogenic shock caused by atrioventficular junctional rhythm immediately after percutaneous coronary intervention (PCI) is described. Pharmacotherapy was invalid, and subse... An 82-year-old female patient undergoing cardiogenic shock caused by atrioventficular junctional rhythm immediately after percutaneous coronary intervention (PCI) is described. Pharmacotherapy was invalid, and subsequent application of atrial pacing reversed the cardiogenic shock. PCI-related injury of sinuatrial nodal artery leading to acute atrial contractility loss, accompanied by atrioventricular junctional arrhythmia, was diagnosed. We recommend that preoperative risk evaluation be required for multi-risk patients. Likewise, emergent measures should to be established in advance. This case reminds us that atrial pacing can be an optimal management technique once cardiogenic shock has occurred. 展开更多
关键词 Cardiogenic shock Atrioventricular junctional rhythm Percutaneous coronary intervention Atrial pacing
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Effect of omeprazole on symptoms and ultrastructural esophageal damage in acid bile reflux 被引量:3
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作者 Carlo Calabrese Anna Fabbri +5 位作者 Mauro Bortolotti Giovanna Cenacchi Scialpi Carlo Desiree Zahlane Mario Miglioli Giulio Di Febo 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第12期1876-1880,共5页
AIM: To value whether omeprazole could induce the healing of DIS and regression of symptoms in patients with DGER. METHODS: We enrolled 15 symptomatic patients with a pathological esophageal 24-h pH-metry and bilimetr... AIM: To value whether omeprazole could induce the healing of DIS and regression of symptoms in patients with DGER. METHODS: We enrolled 15 symptomatic patients with a pathological esophageal 24-h pH-metry and bilimetry. Patients underwent endoscopy and biopsies were taken from the distal esophagus. Specimens were analyzed at histology and transmission electron microscopy (TEM). Patients were treated with omeprazole 40 mg/d for 3 mo and then endoscopy with biopsies was repeated. Patients with persistent heartburn and/or with an incomplete recovery of DIS were treated for 3 more months and endoscopy with biopsies was performed. RESULTS: Nine patients had a non-erosive reflux disease at endoscopy (NERD) while 6 had erosive esophagitis (ERD). At histology, of the 6 patients with erosive esophagitis, 5 had mild esophagitis and 1 moderate esophagitis. No patients with NERD showed histological signs of esophagitis. After 3 mo of therapy, 13/15 patients (86.7%,P<0.01) showed a complete recovery of DIS and disappearance of heartburn. Of the 2 patients treated for 3 more months, complete recovery of DIS and heartburn were achieved in one. CONCLUSION: Three or 6 mo of omeprazole therapy led to a complete regression of the ultrastructural esophageal damage in 86.7% and in 93% of patients with DGER, NERD and ERD respectively. The ultrastructural recovery of the epithelium was accompanied by regression of heartburn in all cases. 展开更多
关键词 Duodenogastroesophageal reflux Gastroeso phageal reflux disease Transmission Electron Microscopy Dilated Intercellular Spaces Non-erosive reflux disease
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An Assessment of the Level of Knowledge of HIV-Infected Patients about Highly Active Antireteroviral Therapy and Waiting Times and Their Influence on Antiretroviral Therapy Adherence at a Primary Healthcare Centre of South Africa 被引量:1
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作者 N L Katende-Kyenda 《Journal of Pharmacy and Pharmacology》 2017年第7期467-477,共11页
Objectives: The study assessed if the level of knowledge of HIV-infected about HAART and waiting-times in the PHC (primary healthcare) clinic have an influence on antiretroviral adherence. Methods: A descriptive-c... Objectives: The study assessed if the level of knowledge of HIV-infected about HAART and waiting-times in the PHC (primary healthcare) clinic have an influence on antiretroviral adherence. Methods: A descriptive-cross-sectional study was conducted in South Africa. Data collected uses a standardized-questionnaire and face-to-face-exit interviews. Pill-count technique was performed and a value of≥ 95% acceptable. Data were analysed using SPSS. Univariate-factors associated with poor-adherence to knowledge about HAART and waiting times were assessed using ANOVA and p ≤ 0.05 considered statistically significant. Key findings: Of 86 enrolled, 63(73.3%) were females and 23(26.7%) males, with mean-age (± SD) of 35.6(±9.6) years and on HAART for 35.5(± 31.8) months ranging from 1-137. Of these, 27(31.40%) and 25(29.07%) were on WHO stages 2 and 3 respectively. Adherence-rates computed from 32 patients, 23(71.9%) revealed poor adherence-rates. The level of knowledge about HAART in terms of names of tablets, correct-dose, frequency, adverse-effects had no influence on ARV-adherence (p _〉 0.05). Of 23 non-compliant, 10 (40%) gave the reason of drugs-unavailability, 7(30%) adverse-effects, 5(20%) drugs' complexity, and 1(10%) too busy to take them. Waiting areas associated with poor ARV-adherence were reception (p = 0.028), doctors (p = 0.027), while nurse's station (p = 0.29) and pharmacy (p = 0.43) revealed acceptable ARV-adherence. 展开更多
关键词 HIV-infected patients highly active antiretroviral drugs ADHERENCE primary health care.
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Reversal of pulmonary vein remodeling after catheter ablation of atrial fibrillation
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作者 Jia-Hui WU Hung-Kei LI +5 位作者 Daniel M Couri Philip A Araoz Ying-Hsiang Lee Chang-Sheng MA Douglas L Packer Yong-Mei CHA 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2016年第2期163-168,共6页
Background Pulmonary veins (PV) and the atria undergo electrical and structural remodeling in atrial fibrillation (AF). This study aimed to determine PV and left atrial (LA) reverse remodeling after catheter abl... Background Pulmonary veins (PV) and the atria undergo electrical and structural remodeling in atrial fibrillation (AF). This study aimed to determine PV and left atrial (LA) reverse remodeling after catheter ablation for AF assessed by chest computed tomography (CT). Methods PV electrophysiologic studies and catheter ablation were performed in 63 patients (68% male; mean ± SD age: 56 ± 10 years) with symptomatic AF (49% paroxysmal, 51% persistent). Chest CT was performed before and 3 months after catheter ablation. Results At baseline, patients with persistent AF had a greater LA volume (91 ±29 cm3 vs. 66 ± 27 cm3; P = 0.003) and mean PV ostial area (241 + 43 mm2 vs. 212 ± 47 mm2; P = 0.03) than patients with paroxysmal AF. There was no significant correlation between the effective refractory period and the area of the left superior PV ostium. At 3 months of follow-up after ablation, 48 patients (76%) were AF free on or off antiarrhythmic drugs. There was a significant reduction in LA volume (77 ±31 cm3 to 70 ± 28 cm3; P 〈 0.001) and mean PV ostial area (224 ± 48 mm2 to 182 ± 43 mm2; P 〈 0.001). Patients with persistent AF had more reduction in LA volume (11.8 ± 12.8 cm3 vs. 4.0 ± 11.2 cm3; P = 0.04) and PV ostial area (62 mm2 vs. 34 mm2; P = 0.04) than those who have paroxysmal AF. The reduction of the averaged PV ostial area was significantly correlated with the reduction of LA volume (r = 0.38, P = 0.03). Conclusions Catheter ablation of AF improves structural remodeling ofPV ostia and left atrium. This finding is more apparent in patients with persistent AF treated by catheter ablation. 展开更多
关键词 Ablation Atrial fibrillation Computed tomography Left atrium Pulmonary vein isolation Pulmonary vein ostial area
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Experimental Study on Airflow Reversal Within Bypass Branch During Mine Fire 被引量:1
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作者 周福宝 王德明 周延 《Journal of China University of Mining and Technology》 2001年第2期95-99,共5页
Based on the analysis of conditions for airflow reversal within a bypass branch, the airflow reversal phenomena were successfully simulated in the laboratory experiments. Data obtained indicate that airflow reversal w... Based on the analysis of conditions for airflow reversal within a bypass branch, the airflow reversal phenomena were successfully simulated in the laboratory experiments. Data obtained indicate that airflow reversal within a bypass branch is mainly caused by fire pressure yielded in the combustion branch. In addition, the maximum reversal quantity of airflow occurs in step with the highest temperature of fire, the highest concentration of CO 2 and the lowest concentration of O 2. Moreover, the time that fire size reaches the maximum value corresponds to the time airflow reversal in the bypass branch is most possible. The results are of great significance for guiding rescue work to avoid airflow reversal. 展开更多
关键词 mine fire ascensional ventilation bypass branch airflow reversal
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Treatment of uncomplicated reflux disease 被引量:3
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作者 Joachim Labenz Peter Malfertheiner 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第28期4291-4299,共9页
Uncomplicated reflux disease comprises the non-erosive reflux disease (NERD) and erosive reflux disease (ERD).The objectives of treatment are the adequate control of symptoms with restoration of quality of life, heali... Uncomplicated reflux disease comprises the non-erosive reflux disease (NERD) and erosive reflux disease (ERD).The objectives of treatment are the adequate control of symptoms with restoration of quality of life, healing of lesions and prevention of relapse. Treatment of NERD consists in the administration of proton pump inhibitors (PPI) for 2-4 wk, although patients with NERD show an overall poorer response to PPI treatment than patients with ERD owing to the fact that patients with NERD do not form a pathophysiologically homogenous group. For long-term management on-demand treatment with a PPI is probably the best option. In patients with ERD, therapy with a standard dose PPI for 4-8 wk is always recommended.Long-term treatment of ERD is applied either intermittently or as continuous maintenance treatment with an attempt to reduce the daily dosage of the PPI (step-down principle).In selected patients requiring long-term PPI treatment,antireflux surgery is an alternative option. In patients with troublesome reflux symptoms and without alarming features empirical PPI therapy is another option for initial management. Therapy should be withdrawn after initial success. In the case of relapse, the long-term care depends on a careful risk assessment and the response to PPI therapy. 展开更多
关键词 Erosive reflux disease Non-erosive reflux disease Proton pump inhibitor Uninvestigated reflux disease
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Personal Experience in TCM Treatment of Reflux Esophagitis
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作者 洪流 毛树章 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2003年第3期175-176,共2页
Reflux esophagitis falls into the category of reflux gastroesophagopathy. It is characterized by inflammation, erosion, ulcer formation and fibrosis of the esophageal mucous membrane due to reflux of the content of th... Reflux esophagitis falls into the category of reflux gastroesophagopathy. It is characterized by inflammation, erosion, ulcer formation and fibrosis of the esophageal mucous membrane due to reflux of the content of the stomach and duodenum into the esophagus. 展开更多
关键词 PHYTOTHERAPY Drugs Chinese Herbal Esophagitis Peptic FEMALE Humans MALE Middle Aged
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Over Exposure to Efavirenz Plasma Concentrations among Beninese HIV Patients Treated by A 600 mg Daily Dose
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作者 Allabi Aurel Constant Kiki Mikal +2 位作者 Zannou Marcel Onifade El-Fatai Alvarez Jean Claude 《Journal of Pharmacy and Pharmacology》 2017年第9期628-635,共8页
Therapeutic plasma concentrations of EFV (efavirenz) are between 1,000 ng/mL and 4,000 ng/mL. Concentrations below 1000 ng/mL are associated with higher risk of treatment failure, and concentrations above 4,000 ng/m... Therapeutic plasma concentrations of EFV (efavirenz) are between 1,000 ng/mL and 4,000 ng/mL. Concentrations below 1000 ng/mL are associated with higher risk of treatment failure, and concentrations above 4,000 ng/mL are associated with toxicity. The aim of the study was to appreciate EFV plasmas concentrations profile and the association between plasma levels and various characteristics in Beninese patients treated by a 600 mg standard daily-dose. Blood samples were collected and EFV plasma levels were measured by liquid chromatography coupled with mass spectrometry detection in HIV-infected patients receiving EFV in combination with other antiretroviral drugs for at least 14 days. Adverse effects occurring during treatment were collected through a questionnaire. An over-exposure to EFV among Beninese HIV patients were observed, with 46.4% of patients presenting EFV concentration above 4,000 ng/mL, although adverse effects were tolerated indicating that antiretroviral treatment is safe. The measurement of plasma concentration at the steady-state could contribute to early detection of treatment failure and adapt treatment in subjects presenting serious adverse effects within context of therapeutic drug monitoring. 展开更多
关键词 HIV EFAVIRENZ plasma concentrations adverse effects Benin.
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Specific activation of 2'-5'oligoadenylate synthetase gene promoter by hepatitis C virus-core protein:A potential for developing hepatitis C virus targeting gene therapy
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作者 Ying Wang Shan-Shan Mao +3 位作者 Qiong-Qiong He Yuan Zi Ji-Fang Wen De-Yun Feng 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第25期3178-3182,共5页
AIM: TO examine whether 2'-5'oligoadenylate synthetase (OAS) gene promoter can be specifically activated by hepatitis C virus (HCV)-core protein. METHODS: Human embryo hepatic cell line L02 was transfected wit... AIM: TO examine whether 2'-5'oligoadenylate synthetase (OAS) gene promoter can be specifically activated by hepatitis C virus (HCV)-core protein. METHODS: Human embryo hepatic cell line L02 was transfected with pcDNA3.1-core plasmid and selected by G418. Expression of HCV-core was detected by reverse transcription polymerase chain reaction and Western blotting. The OAS promoter sequence was amplified from the genomic DNA and inserted into pGL3-basic vector. The resultant pGL3-OAS-Luci plasmid was transiently transfected into L02/core cells and luciferase activity was assayed. I^ESULTS: L02/core cell line stably expressing HCV- core protein was established. The pGL3-OAS-Luci construct exhibited significant transcriptional activity in the L02/core cells but not in the L02 cells. CONCLUSION: HCV-core protein activates the OAS gene promoter specifically and effectively. Utilization of OAS gene promoter would be an ideal strategy for developing HCV-specific gene therapy. 展开更多
关键词 Hepatitis C virus Gene promoter Gene therapy CORE 2'-5'oligoadenylate synthetase
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