Objective:The aim of the research was to study the effects of prolactin-inducible protein(PIP) downregulation on metastatic abilities of human breast cancer MDA-MB-453 cells.Methods:PIP-siRNA was transfected into huma...Objective:The aim of the research was to study the effects of prolactin-inducible protein(PIP) downregulation on metastatic abilities of human breast cancer MDA-MB-453 cells.Methods:PIP-siRNA was transfected into human breast cancer MDA-MB-453 cells through liposome.Reverse transcription PCR and immunocytochemistry were employed to detect the downregulated expression of PIP.Cell migration,adhesion and invasion assays were performed to assess the impacts of PIP downregulation on cell migration,adhesion and invasion respectively.Results:Knockdown of PIP obviously inhibited cell migration,the migrated cells were decreased by 83.1% compared with the negative control group.Cell adhesion was also reduced,the adhesion rates at 30 min and 60 min were decreased by 42.6% and 48.5% respectively compared with the negative control group.Moreover,PIP downregulation resulted in decreased invasion rate by 73.9%.Conclusion:Reduced PIP expression in MDA-MB-453 cells can inhibit the abilities of migration,adhesion and invasion,which suggests that PIP plays an important role in the metastatic potency of breast cancer cells.展开更多
By controlling the amorphous-to-crystalline relative volume,chalcogenide phase-change memory materials can provide multi-level data storage(MLS),which offers great potential for high-density storageclass memory and ne...By controlling the amorphous-to-crystalline relative volume,chalcogenide phase-change memory materials can provide multi-level data storage(MLS),which offers great potential for high-density storageclass memory and neuro-inspired computing.However,this type of MLS system suffers from high power consumption and a severe time-dependent resistance increase(‘‘drift")in the amorphous phase,which limits the number of attainable storage levels.Here,we report a new type of MLS system in yttriumdoped antimony telluride,utilizing reversible multi-level phase transitions between three states,i.e.,amorphous,metastable cubic and stable hexagonal crystalline phases,with ultralow power consumption(0.6–4.3 p J)and ultralow resistance drift for the lower two states(power-law exponent<0.007).The metastable cubic phase is stabilized by yttrium,while the evident reversible cubic-to-hexagonal transition is attributed to the sequential and directional migration of Sb atoms.Finally,the decreased heat dissipation of the material and the increase in crystallinity contribute to the overall high performance.This study opens a new way to achieve advanced multi-level phase-change memory without the need for complicated manufacturing procedures or iterative programming operations.展开更多
The South China Sea where water depth is up to 5000 m is the most promising oil and gas exploration area in China in the future.The seismic data acquired in the South China Sea contain various types of multiples that ...The South China Sea where water depth is up to 5000 m is the most promising oil and gas exploration area in China in the future.The seismic data acquired in the South China Sea contain various types of multiples that need to be removed before imaging can be developed.However,compared with the conventional reflection migration,multiples carry more information of the underground structure that helps provide better subsurface imaging.This paper presents a method to modify the conventional reverse time migration so that multiple reflections can migrate to their correct locations in the subsurface.This approach replaces the numerical impulsive source with the recorded data including primaries and multiples on the surface,and replaces the recorded primary reflection data with multiples.In the reverse time migration process,multiples recorded on the surface are extrapolated backward in time to each depth level,while primaries and multiples recorded on the surface are extrapolated forward in time to the same depth levels.By matching the difference between the primary and multiple images using an objective function,this algorithm improves the primary resultant image.Synthetic tests on Sigsbee2 B show that the proposed method can obtain a greater range and better underground illumination.Images of deep water in the South China Sea are obtained using multiples and their matching with primaries.They demonstrate that multiples can make up for the reflection illumination and the migration of multiples is an important research direction in the future.展开更多
The results of experimental investigation of laminar-turbulent transition in three-dimensional flow under the high continuous pressure gradient including the flow with local boundary layer separation are presented. Th...The results of experimental investigation of laminar-turbulent transition in three-dimensional flow under the high continuous pressure gradient including the flow with local boundary layer separation are presented. The experimental studies were performed within the Mach number range from 4 to 6 and Reynolds number 10-60×106 1/m, the angles of attack were 0° and 5°. The experiments were carried out on the three-dimensional convergent inlet model with and without sidewalls. The influence of artificial tubulator of boundary layer on transition and flow structure was studied. The conducted researches have shown that adverse pressure gradient increase hastens transition and leads to decrease of transition area length. If pressure gradient rises velocity profile fullness increases and profile transformation from laminar to turbulent occurs. As a result of it the decrease of separation area length occurs. The same effect was reached with Reynolds number increase. These results are compared with the data on two-dimensional model with longitudinal curvature.展开更多
Human endogenous retrovirus W env(HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis(MS). These diseases are accompanied by immunological reactions in th...Human endogenous retrovirus W env(HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis(MS). These diseases are accompanied by immunological reactions in the central nervous system(CNS). Microglia are important immunocytes in brain inflammation that can produce a gasotransmitter – nitric oxide(NO). NO not only plays a role in the function of neuronal cells but also participates in the pathogenesis of various neuropsychological diseases. In this study, we reported increased NO production in CHME-5 microglia cells after they were transfected with HERV-W env. Moreover, HERV-W env increased the expression and function of human inducible nitric oxide synthase(hi NOS) and enhanced the promoter activity of hi NOS. Microglial migration was also enhanced. These data revealed that HERV-W env might contribute to increase NO production and microglial migration ability in neuropsychological disorders by regulating the expression of inducible NOS. Results from this study might lead to the identification of novel targets for the treatment of neuropsychological diseases, including neuroinflammatory diseases, stroke, and neurodegenerative diseases.展开更多
文摘Objective:The aim of the research was to study the effects of prolactin-inducible protein(PIP) downregulation on metastatic abilities of human breast cancer MDA-MB-453 cells.Methods:PIP-siRNA was transfected into human breast cancer MDA-MB-453 cells through liposome.Reverse transcription PCR and immunocytochemistry were employed to detect the downregulated expression of PIP.Cell migration,adhesion and invasion assays were performed to assess the impacts of PIP downregulation on cell migration,adhesion and invasion respectively.Results:Knockdown of PIP obviously inhibited cell migration,the migrated cells were decreased by 83.1% compared with the negative control group.Cell adhesion was also reduced,the adhesion rates at 30 min and 60 min were decreased by 42.6% and 48.5% respectively compared with the negative control group.Moreover,PIP downregulation resulted in decreased invasion rate by 73.9%.Conclusion:Reduced PIP expression in MDA-MB-453 cells can inhibit the abilities of migration,adhesion and invasion,which suggests that PIP plays an important role in the metastatic potency of breast cancer cells.
基金the National Key Research and Development Program of China(2017YFB0701700)the National Natural Science Foundation of China(51872017)the High-Performance Computing(HPC)Resources at Beihang University。
文摘By controlling the amorphous-to-crystalline relative volume,chalcogenide phase-change memory materials can provide multi-level data storage(MLS),which offers great potential for high-density storageclass memory and neuro-inspired computing.However,this type of MLS system suffers from high power consumption and a severe time-dependent resistance increase(‘‘drift")in the amorphous phase,which limits the number of attainable storage levels.Here,we report a new type of MLS system in yttriumdoped antimony telluride,utilizing reversible multi-level phase transitions between three states,i.e.,amorphous,metastable cubic and stable hexagonal crystalline phases,with ultralow power consumption(0.6–4.3 p J)and ultralow resistance drift for the lower two states(power-law exponent<0.007).The metastable cubic phase is stabilized by yttrium,while the evident reversible cubic-to-hexagonal transition is attributed to the sequential and directional migration of Sb atoms.Finally,the decreased heat dissipation of the material and the increase in crystallinity contribute to the overall high performance.This study opens a new way to achieve advanced multi-level phase-change memory without the need for complicated manufacturing procedures or iterative programming operations.
基金supported by the National Basic Research Program of China(Grant No.2009CB219405)the National Oil and Gas Program(Grant No.2011ZX05008-006)the National Natural Science Foundation of China(Grant Nos.40930421,41074091)
文摘The South China Sea where water depth is up to 5000 m is the most promising oil and gas exploration area in China in the future.The seismic data acquired in the South China Sea contain various types of multiples that need to be removed before imaging can be developed.However,compared with the conventional reflection migration,multiples carry more information of the underground structure that helps provide better subsurface imaging.This paper presents a method to modify the conventional reverse time migration so that multiple reflections can migrate to their correct locations in the subsurface.This approach replaces the numerical impulsive source with the recorded data including primaries and multiples on the surface,and replaces the recorded primary reflection data with multiples.In the reverse time migration process,multiples recorded on the surface are extrapolated backward in time to each depth level,while primaries and multiples recorded on the surface are extrapolated forward in time to the same depth levels.By matching the difference between the primary and multiple images using an objective function,this algorithm improves the primary resultant image.Synthetic tests on Sigsbee2 B show that the proposed method can obtain a greater range and better underground illumination.Images of deep water in the South China Sea are obtained using multiples and their matching with primaries.They demonstrate that multiples can make up for the reflection illumination and the migration of multiples is an important research direction in the future.
文摘The results of experimental investigation of laminar-turbulent transition in three-dimensional flow under the high continuous pressure gradient including the flow with local boundary layer separation are presented. The experimental studies were performed within the Mach number range from 4 to 6 and Reynolds number 10-60×106 1/m, the angles of attack were 0° and 5°. The experiments were carried out on the three-dimensional convergent inlet model with and without sidewalls. The influence of artificial tubulator of boundary layer on transition and flow structure was studied. The conducted researches have shown that adverse pressure gradient increase hastens transition and leads to decrease of transition area length. If pressure gradient rises velocity profile fullness increases and profile transformation from laminar to turbulent occurs. As a result of it the decrease of separation area length occurs. The same effect was reached with Reynolds number increase. These results are compared with the data on two-dimensional model with longitudinal curvature.
基金supported by grants from the National Natural Sciences Foundation of China(No.31470264,No.81271820,No.30870789,and No.30300117)the Key Program of Natural Science Foundation of Hubei Province of China(No.2014CFA078)+1 种基金the Stanley Foundation from the Stanley Medical Research Institute(SMRI),USA(No.06R-1366),to Dr.Fan Zhuthe Scientific Innovation Team Project of Hubei Province of China(No.2015CFA009)
文摘Human endogenous retrovirus W env(HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis(MS). These diseases are accompanied by immunological reactions in the central nervous system(CNS). Microglia are important immunocytes in brain inflammation that can produce a gasotransmitter – nitric oxide(NO). NO not only plays a role in the function of neuronal cells but also participates in the pathogenesis of various neuropsychological diseases. In this study, we reported increased NO production in CHME-5 microglia cells after they were transfected with HERV-W env. Moreover, HERV-W env increased the expression and function of human inducible nitric oxide synthase(hi NOS) and enhanced the promoter activity of hi NOS. Microglial migration was also enhanced. These data revealed that HERV-W env might contribute to increase NO production and microglial migration ability in neuropsychological disorders by regulating the expression of inducible NOS. Results from this study might lead to the identification of novel targets for the treatment of neuropsychological diseases, including neuroinflammatory diseases, stroke, and neurodegenerative diseases.