Ion-sensitive field-effect transistor （ISFET）-based biosensor for amino acids was proposed, and the response of the sensor to amino acids was evaluated. As a molecular recognition element, aminoacyl-tRNA synthetase ...Ion-sensitive field-effect transistor （ISFET）-based biosensor for amino acids was proposed, and the response of the sensor to amino acids was evaluated. As a molecular recognition element, aminoacyl-tRNA synthetase which would be expected to have high selectivity for the corresponding amino acids was used, aminoacyl-tRNA synthetase was coated onto an ISFET electrode, and the response of the biosensor to amino acids was evaluated. The amino acid sensor for tyrosine showed a selective response to tyrosine because of the specific binding ability of aminoacyl-tRNA synthetase for tyrosine and from 300μM to 900 μM of tyrosine could be measured （r2 〉 0.969）.展开更多
A non-sulfur chemosensor based on an easy to prepare double naphthalene Schiff base is reported for the colorimetric and fluorometric dual-channel sensing of Hg2+ ions by taking advantage of the twisted intramolecular...A non-sulfur chemosensor based on an easy to prepare double naphthalene Schiff base is reported for the colorimetric and fluorometric dual-channel sensing of Hg2+ ions by taking advantage of the twisted intramolecular charge transfer (TICT) mechanism. This work provides a novel approach for the selective recognition of mercury ions. Moreover, this sensor serves as a potential recyclable component in sensing materials and the complex L-Hg2+ (L = 1-[(2-naphthalenylimino)methyl]-2-naphthalenol) can therefore be used as a fluorescent sensor for iodine anions. Notably, the color changes are very significant and all the recognition and recycling processes can be observed by the naked eye.展开更多
A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and...A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and up to 700-fold enhancement in two-photon photoluminescence.Huge enhancement in two-photon photoluminescence allows achieving a detection limit of as low as 0.0062 mg/kg,which is better than the conventional colorimetric methods.This two-photon sensing scheme has a broad dynamic range and displays excellent selectivity in detection of cartap against over other ten kinds of commonly used insecticides.展开更多
An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of ...An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.展开更多
The development of sensors for selective detection of cyanide ion(CN^-) is an important mission to accomplish because of the versatility and toxicity of CN^-. In the present work, an "ensemble"-based colorim...The development of sensors for selective detection of cyanide ion(CN^-) is an important mission to accomplish because of the versatility and toxicity of CN^-. In the present work, an "ensemble"-based colorimetric and fluorescent sensor(L2-Zn^(2+)) for CN^-ion has been developed. The addition of cyanide ions removed Zn^(2+) from the ensemble(L2-Zn^(2+)) in aqueous medium, resulting in a color change of the solution from red to buff and a "turn-on" fluorescent response. Also, the sensitivity of both the fluorescenceand colorimetric-based assay is below the maximum allowable level of cyanide ions in drinking water set by the World Health Organization. In addition, test strips, which served as convenient and efficient CN^- test kits, were fabricated based on the sensor.Notably, the selective detection of cyanide with L2-Zn^(2+) for practical application was also performed in sprouting potatoes.展开更多
文摘Ion-sensitive field-effect transistor （ISFET）-based biosensor for amino acids was proposed, and the response of the sensor to amino acids was evaluated. As a molecular recognition element, aminoacyl-tRNA synthetase which would be expected to have high selectivity for the corresponding amino acids was used, aminoacyl-tRNA synthetase was coated onto an ISFET electrode, and the response of the biosensor to amino acids was evaluated. The amino acid sensor for tyrosine showed a selective response to tyrosine because of the specific binding ability of aminoacyl-tRNA synthetase for tyrosine and from 300μM to 900 μM of tyrosine could be measured （r2 〉 0.969）.
基金supported by the National Natural Science Foundation of China (21064006, 21161018)the Natural Science Foundation of Gansu Province (1010RJZA018)the Program for Changjiang Scholars and Innovative Research Teams in Universities of the Ministry of Education of China (IRT1177)
文摘A non-sulfur chemosensor based on an easy to prepare double naphthalene Schiff base is reported for the colorimetric and fluorometric dual-channel sensing of Hg2+ ions by taking advantage of the twisted intramolecular charge transfer (TICT) mechanism. This work provides a novel approach for the selective recognition of mercury ions. Moreover, this sensor serves as a potential recyclable component in sensing materials and the complex L-Hg2+ (L = 1-[(2-naphthalenylimino)methyl]-2-naphthalenol) can therefore be used as a fluorescent sensor for iodine anions. Notably, the color changes are very significant and all the recognition and recycling processes can be observed by the naked eye.
基金the Ministry of Education, Singapore (R-143-000-607-112)the National Research Foundation, Prime Minister’s Office, Singapore under its Competitive Research Program (NRF-CRP10-2012-04)Medium Sized Centre Programme
文摘A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and up to 700-fold enhancement in two-photon photoluminescence.Huge enhancement in two-photon photoluminescence allows achieving a detection limit of as low as 0.0062 mg/kg,which is better than the conventional colorimetric methods.This two-photon sensing scheme has a broad dynamic range and displays excellent selectivity in detection of cartap against over other ten kinds of commonly used insecticides.
基金supported by the National Natural Science Foundation of China (20275025 & 20675055)the Natural Science Fundation of Jiangsu Province (BK2009111)Technology Plan of Suzhou (SYJG0901)
文摘An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.
基金supported by the National Natural Science Foundation of China(21662031,21661028,21574104,21262032)the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(IRT 15R56)
文摘The development of sensors for selective detection of cyanide ion(CN^-) is an important mission to accomplish because of the versatility and toxicity of CN^-. In the present work, an "ensemble"-based colorimetric and fluorescent sensor(L2-Zn^(2+)) for CN^-ion has been developed. The addition of cyanide ions removed Zn^(2+) from the ensemble(L2-Zn^(2+)) in aqueous medium, resulting in a color change of the solution from red to buff and a "turn-on" fluorescent response. Also, the sensitivity of both the fluorescenceand colorimetric-based assay is below the maximum allowable level of cyanide ions in drinking water set by the World Health Organization. In addition, test strips, which served as convenient and efficient CN^- test kits, were fabricated based on the sensor.Notably, the selective detection of cyanide with L2-Zn^(2+) for practical application was also performed in sprouting potatoes.
