Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we use...Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we used a rat model of carotid artery balloon injury （CABI） to study the changes and potential roles of vascular TCs after vascular injury. Transmission electron microscopy （TEM） and CD34/vimentin immunolabeling were used to identify and quantify TCs in normal and injured carotid arteries. Quantita- tive immunofluorescence analysis revealed that, compared with the sham group, the number of TCs in the CAB1 group in- creased from 7.2＋1.0 to an average of 20.4＋1.8 per l-ram2 vascular area. The expression level of miR-24 in TCs was three times higher than in vascular smooth muscle cells （VSMCs）. The percentage of VSMCs in S phase and G2/M phase increased by approximately 5% when VSMCs were incubated with the supematant of TCs. The antagomir of miR-24 in TCs reduced the ratio of VSMCs in S ohase and G2/M phase. This study illuminates the function of TCs in the proliferation of VSMCs.展开更多
OBJECTIVE:To study the effect of Lichong Decoction(Lichong Decoction for strengthening anti-pathogenic Qi and eliminating blood stasis) on the expression of insulin-like growth factor-I(IGF-I) and proliferating cell n...OBJECTIVE:To study the effect of Lichong Decoction(Lichong Decoction for strengthening anti-pathogenic Qi and eliminating blood stasis) on the expression of insulin-like growth factor-I(IGF-I) and proliferating cell nuclear antigen(PCNA) mRNA in a rat model of uterine leiomyoma.METHODS:Fifty female Wistar rats were randomized into a normal control group,model group,Lichong Decoction group,Guizhifuling Capsule(Capsule containing Cassia Twig and Poria) group,and Mifepristone group.The uterine leiomyoma model was established by peritoneal injections of exogenous estrogen and progesterone hormone.The ultrastructural changes in cells of rat uterine tissues were observed with transmission electron microscopy,and the expression of IGF-I and PCNA mRNA was detected by real-time fluorescent quantitative PCR.RESULTS:Following treatment,cells in the Lichong Decoction group appeared to be arranged normally,the cellular morphology were almost in a normal state,hyperplasia and hypertrophy of the chondriosome was reduced,collagen fibers were arranged in a regular manner,without obvious hyperplasia,and the expression of IGF-I and PCNA mRNA was significantly decreased compared with the model group(P<0.01).CONCLUSIONS:The effect of Lichong Decoction on uterine leiomyoma is related to its function in reducing the expression of IGF-I and PCNA mRNA.展开更多
基金supported by the National Natural Science Foundation of China (91339105)the Beijing Natural Science Foundation (7142165)the Science and Technology Commission of Beijing Municipality (z141100000214006)
文摘Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we used a rat model of carotid artery balloon injury （CABI） to study the changes and potential roles of vascular TCs after vascular injury. Transmission electron microscopy （TEM） and CD34/vimentin immunolabeling were used to identify and quantify TCs in normal and injured carotid arteries. Quantita- tive immunofluorescence analysis revealed that, compared with the sham group, the number of TCs in the CAB1 group in- creased from 7.2＋1.0 to an average of 20.4＋1.8 per l-ram2 vascular area. The expression level of miR-24 in TCs was three times higher than in vascular smooth muscle cells （VSMCs）. The percentage of VSMCs in S phase and G2/M phase increased by approximately 5% when VSMCs were incubated with the supematant of TCs. The antagomir of miR-24 in TCs reduced the ratio of VSMCs in S ohase and G2/M phase. This study illuminates the function of TCs in the proliferation of VSMCs.
基金Supported by the National Natural Science Foundation of China(No.81073096)the Natural Science Foundation of Beijing City(No.7082015)the "Cultivation Plan for Youth Backbone Talents" Project(PHR 201008403) of Higher SchoolTalent Strong Education Plan
文摘OBJECTIVE:To study the effect of Lichong Decoction(Lichong Decoction for strengthening anti-pathogenic Qi and eliminating blood stasis) on the expression of insulin-like growth factor-I(IGF-I) and proliferating cell nuclear antigen(PCNA) mRNA in a rat model of uterine leiomyoma.METHODS:Fifty female Wistar rats were randomized into a normal control group,model group,Lichong Decoction group,Guizhifuling Capsule(Capsule containing Cassia Twig and Poria) group,and Mifepristone group.The uterine leiomyoma model was established by peritoneal injections of exogenous estrogen and progesterone hormone.The ultrastructural changes in cells of rat uterine tissues were observed with transmission electron microscopy,and the expression of IGF-I and PCNA mRNA was detected by real-time fluorescent quantitative PCR.RESULTS:Following treatment,cells in the Lichong Decoction group appeared to be arranged normally,the cellular morphology were almost in a normal state,hyperplasia and hypertrophy of the chondriosome was reduced,collagen fibers were arranged in a regular manner,without obvious hyperplasia,and the expression of IGF-I and PCNA mRNA was significantly decreased compared with the model group(P<0.01).CONCLUSIONS:The effect of Lichong Decoction on uterine leiomyoma is related to its function in reducing the expression of IGF-I and PCNA mRNA.
