OBJECTIVE: To investigate the possible advantages of acupotomy over sodium hyaluronate injection for the treatment of knee osteoarthritis(KOA).METHODS: Twenty rabbits were divided randomly into four groups(n = 5 in ea...OBJECTIVE: To investigate the possible advantages of acupotomy over sodium hyaluronate injection for the treatment of knee osteoarthritis(KOA).METHODS: Twenty rabbits were divided randomly into four groups(n = 5 in each): a control group,model group, acupotomy group, and sodium hyaluronate injection group. The model, acupotomy,and sodium hyaluronate groups underwent anterior cruciate ligament transection plus partial medial meniscectomy. Sodium hyaluronate injection and acupotomy were administered to the respective groups from weeks 5 to 8, and samples of the tibial plateau and medial condyle of the femur were collected in week 9. Vascular endothelial growth factor(VEGF) expression was assessed in cartilage and subchondral bone by immunohistochemical staining.RESULTS: Articular cartilage degeneration was less pronounced in the acupotomy compared with the model and sodium hyaluronate groups. VEGF expression levels in cartilage and subchondral bone were increased in the model group compared with the control group(P < 0.01), and acupotomy had a more pronounced therapeutic effect than sodium hyaluronate injection(P < 0.01).CONCLUSION: Acupotomy and sodium hyaluronate injection may both reduce degeneration in the cartilage and subchondral bone in KOA based on the results from a rabbit model, but acupotomy improved the histopathology and reduced the VEGF content more effectively than sodium hyaluronate injection, probably by reducing venous stasis and intraosseous pressure. Acupotomy may improve KOA by lowering VEGF.展开更多
Objective: To observe the influence of intra-articular injection of sodium hyaluronate (HA) on the mRNA expressions of matrix metalloproteinase-1,-3 (MMP-1,-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in c...Objective: To observe the influence of intra-articular injection of sodium hyaluronate (HA) on the mRNA expressions of matrix metalloproteinase-1,-3 (MMP-1,-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in cartilage and synovium of traumatic osteoarthritis (OA).Methods: Sixteen white rabbits underwent unilateral anterior cruciate ligament transection (ACLT) were divided into 2 groups randomly 5 weeks after transection. The experimental group rabbits received 0.3 ml of 1% HA by intra-articular injection once a week. Animals in the control group were treated under the same conditions using physiological saline. Ten weeks following surgery, cartilage and synovium were harvested. The mRNA expressions of MMP-1, MMP-3 and TIMP-1 were analyzed using reverse transcription-polymerase chain reaction (RT-PCR).Results: In synovium, the mRNA expression of MMP-3 was suppressed in the HA injection group. HA treatment had no effect on the MMP-3 expression in cartilage. No significant difference of MMP-1 and TIMP-1 expressions in cartilage and synovium was found between the HA injection group and the control group.Conclusions: One of the mechanisms of the therapeutic effect of HA may be the inhibition of expression of MMP-3 in synovium during early stage of traumatic OA.展开更多
This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly,prepare HA with various average molecular weight and ...This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly,prepare HA with various average molecular weight and alginate with different viscosity,mix them up at a certain proportion and make it into a Alg Ca2 +-HA composite scaffold with a film-forming method. This article discusses the feasibility of this scaffold used in tissue engineering field according to the consequence of moisture content testing,mechanical analysis, and scanning electron microscopy analysis. The structure and properties of Alg Ca2 +-HA composite scaffold are closely related to some factors such as average molecular weight of hyaluronic acid, hyaluronic acid concentration, alginate viscosity, cross-linking agents and processing technology. The Alg Ca2+-HA composite material, which is at different proportions and adding different cross-linking agent,has some certain characteristics: moisture content ranging from 50% to 95%, tensile strength between 2.69 N/mm2 and 4.299 N/mm2, and elongation at break is about 58% to 160%.The prepared Alg Ca2+-HA composite scaffolds can be used as tissue engineering scaffolds resulting from its high moisture content, good mechanical properties and ideal pore structure.展开更多
基金Supported by Beijing medicine research Development Fund:A Clinical Study On ltrasound-guided Acupotomy Treatment of Knee Osteoarthritis(No.SF-2009-46)
文摘OBJECTIVE: To investigate the possible advantages of acupotomy over sodium hyaluronate injection for the treatment of knee osteoarthritis(KOA).METHODS: Twenty rabbits were divided randomly into four groups(n = 5 in each): a control group,model group, acupotomy group, and sodium hyaluronate injection group. The model, acupotomy,and sodium hyaluronate groups underwent anterior cruciate ligament transection plus partial medial meniscectomy. Sodium hyaluronate injection and acupotomy were administered to the respective groups from weeks 5 to 8, and samples of the tibial plateau and medial condyle of the femur were collected in week 9. Vascular endothelial growth factor(VEGF) expression was assessed in cartilage and subchondral bone by immunohistochemical staining.RESULTS: Articular cartilage degeneration was less pronounced in the acupotomy compared with the model and sodium hyaluronate groups. VEGF expression levels in cartilage and subchondral bone were increased in the model group compared with the control group(P < 0.01), and acupotomy had a more pronounced therapeutic effect than sodium hyaluronate injection(P < 0.01).CONCLUSION: Acupotomy and sodium hyaluronate injection may both reduce degeneration in the cartilage and subchondral bone in KOA based on the results from a rabbit model, but acupotomy improved the histopathology and reduced the VEGF content more effectively than sodium hyaluronate injection, probably by reducing venous stasis and intraosseous pressure. Acupotomy may improve KOA by lowering VEGF.
文摘Objective: To observe the influence of intra-articular injection of sodium hyaluronate (HA) on the mRNA expressions of matrix metalloproteinase-1,-3 (MMP-1,-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in cartilage and synovium of traumatic osteoarthritis (OA).Methods: Sixteen white rabbits underwent unilateral anterior cruciate ligament transection (ACLT) were divided into 2 groups randomly 5 weeks after transection. The experimental group rabbits received 0.3 ml of 1% HA by intra-articular injection once a week. Animals in the control group were treated under the same conditions using physiological saline. Ten weeks following surgery, cartilage and synovium were harvested. The mRNA expressions of MMP-1, MMP-3 and TIMP-1 were analyzed using reverse transcription-polymerase chain reaction (RT-PCR).Results: In synovium, the mRNA expression of MMP-3 was suppressed in the HA injection group. HA treatment had no effect on the MMP-3 expression in cartilage. No significant difference of MMP-1 and TIMP-1 expressions in cartilage and synovium was found between the HA injection group and the control group.Conclusions: One of the mechanisms of the therapeutic effect of HA may be the inhibition of expression of MMP-3 in synovium during early stage of traumatic OA.
文摘This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly,prepare HA with various average molecular weight and alginate with different viscosity,mix them up at a certain proportion and make it into a Alg Ca2 +-HA composite scaffold with a film-forming method. This article discusses the feasibility of this scaffold used in tissue engineering field according to the consequence of moisture content testing,mechanical analysis, and scanning electron microscopy analysis. The structure and properties of Alg Ca2 +-HA composite scaffold are closely related to some factors such as average molecular weight of hyaluronic acid, hyaluronic acid concentration, alginate viscosity, cross-linking agents and processing technology. The Alg Ca2+-HA composite material, which is at different proportions and adding different cross-linking agent,has some certain characteristics: moisture content ranging from 50% to 95%, tensile strength between 2.69 N/mm2 and 4.299 N/mm2, and elongation at break is about 58% to 160%.The prepared Alg Ca2+-HA composite scaffolds can be used as tissue engineering scaffolds resulting from its high moisture content, good mechanical properties and ideal pore structure.
