To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber techniqu...To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber technique, from Sept 3, 2002 to Oct 30, 2003 in two types of soil ecosystems, of which one was covered with litters on the surface soil, and the other had no litters. The results showed that litters had significant influences on CO2, N2O and CH4 fluxes (p<0.05). Their diurnal change patterns of plot with litters and litter-free plot were similar, and they all showed emission/absorption peak at 18:00. The diurnal change fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the diurnal flux of CH4 of plot with litters was lower than that of litter-free plot. The fluxes of CO2, N2O, and CH4 showed the similar seasonal patternsfor both plots. The fluxes of CO2, CH4 showed their peak fluxes in June, but the fluxes of N2O showed its peak emissions in August. The annual fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the annual flux of CH4 of plot with litters was lower than that of litter-free plot. Keywords Flux - CO2, N2O and CH4 - Seasonal variation - Diurnal variation CLC number S718.5 Document code A Foundation item: The study was supported by innovation research project of Institute of Appiied Ecology, Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (30271068)Bigraphy: XIAO Dong-mei (1979-), female, master of Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016. P. R. China.Responsible editor: Song Funan展开更多
To alleviate the resulting increase in energy consumption and emissions and other issues caused by the traffic congestion ahead of the expressway toll station, a novel traffic flowcontrol method is put forward based o...To alleviate the resulting increase in energy consumption and emissions and other issues caused by the traffic congestion ahead of the expressway toll station, a novel traffic flowcontrol method is put forward based on the environment-friendly conception. The technical thinking of inducing the slowly moving traffic into a batch pass is determined based on the conclusion of the research, traffic flowtheory and traffic sensing detection technology. The model of stop times is established and the parameters of the system are optimized in accordance with the principle of minimizing the fuel consumption. The optimal location selection of traffic control lights and Detector 2 for queue of different lengths at toll stations are calculated based on the model. Finally, the effect of the congestion flowcontrol system is verified via the Paramics simulation system. The result shows that the control system is capable of reducing90% of fuel consumption for vehicles going through toll stations.展开更多
Using the 3-year observational data from ChinaFlux (Chinese Terrestrial Ecosystem Flux Research Network), we studied the gas regulation flux dynamics and cumulative process of gas regulation value in Qianyanzhou mid...Using the 3-year observational data from ChinaFlux (Chinese Terrestrial Ecosystem Flux Research Network), we studied the gas regulation flux dynamics and cumulative process of gas regulation value in Qianyanzhou middle subtropical plantation (QYF) and Changbai Mountain temperate mixed forest (CBF). The gas regulation service was differentiated into vegetation gas regulation service and net ecosystem gas regulation service. Carbon tax approach, reforestation cost approach and industrial oxygen approach were employed to calculate gas regulation value. Results show that there was significant seasonal variation in vegetation gas regulation flux. Daily CO2 uptake fluxes averaged 82.00 kg·ha^-·d^-1 and 59.37 kg·ha^-·d^-1 and the corresponding 02 emission fluxes were 59.65 kg·ha^-·d^-1 and 43.19 kg·ha^-·d^-1 for QYF and CBF, respectively. The cumulative curves of vegetation gas regulation value always followed a sigmoid shape, and the annual gas regulation value produced by vegetation was RMB 14342.69 yuan·ha^-1 and RMB 10384.18 yuan·ha^-1 for both QYF and CBF, respectively. In terms of monthly net ecosystem gas regulation service, QYF appeared as a CO2 sink and O2 source for the whole year, while CBF appeared to be a CO2 sink and O2 source mainly in the period between May and September. The cumulative curves of net ecosystem gas regulation value presented a sigmoid ("S") shape for QYF, while a unimodal type curve for CBF. The annual net ecosystem gas regulation value was 8470.52 yuan·ha^-1 and 5091.98yuan·ha^-1 for QYF and CBF, respectively. The economic value of both the vegetation gas regulation service and net ecosystem gas regulation service were mainly produced between May and October.展开更多
The necessity and feasibility of the use of the personalized ventilation(PV)technology in a toll booth is described.First,the indoor environment of the toll booth equipped with a PV system is analyzed.Based on the a...The necessity and feasibility of the use of the personalized ventilation(PV)technology in a toll booth is described.First,the indoor environment of the toll booth equipped with a PV system is analyzed.Based on the analysis results,a set of equipment for controlling the indoor air quality(IAQ)of the toll booth is devised.Then,a full-scale model of the toll booth is set up in the laboratory.The airflow organization,the optimum operation parameters,and the restraint effects of the PV system on pollution are also experimentally studied.The experimental results on the air supply characteristics show that the PV system can effectively reduce the air age,improve the ventilation efficiency,and enhance the comfort and acceptability of human beings.In addition,this system plays a significant role in preventing pollution.展开更多
Estimation of Rayleigh fading channels using time multiplexed pilot symbols in W CDMA (FDD) is considered. Method of interpolation on a second order curve using the instantaneous channel estimation of two contiguous...Estimation of Rayleigh fading channels using time multiplexed pilot symbols in W CDMA (FDD) is considered. Method of interpolation on a second order curve using the instantaneous channel estimation of two contiguous slots based on least square method (SOLSM) is presented. It is demonstrated by computer simulations that the channel distortion on data symbols in a slot can be estimated effectively compared to the method using simple linear interpolation, and the performance of the Rake combiner is improved effectively especially in fast moving cases.展开更多
2.5 Gbit/s monolithic integrated circuits (ICs) for optical fiber transmitter and receiver in 0.35 μm CMOS (complementary metal-oxide-semiconductor transistor) process are presented. The transmitter, which includ...2.5 Gbit/s monolithic integrated circuits (ICs) for optical fiber transmitter and receiver in 0.35 μm CMOS (complementary metal-oxide-semiconductor transistor) process are presented. The transmitter, which includes a 4: 1 multiplexer and a laser diode driver (LDD), has four 622 Mbit/s random signals as its inputs and gets a 2.5 Gbit/s driving signal as its output; the receiver detects a 2.5 Gbit/s random signal and gets four 622 Mbit/s signals at the output. The main circuits include a trans-impedance amplifier (TIA), a limiting amplifier, a clock and data recovery (CDR) unit, and a 1: 4 demultiplexer (DEMUX). Test results prove the logic functions of the transmitter to be right, and the 10% to 90% rise and fall times of transmitter's output data eye diagram are 211.1 ps and 200 ps, respectively. The sensitivity of the receiver is measured to be better than 20 mV. The root mean square jitter of the DEMUX's output data is 15.6 ps and that of the clock after 1: 4 frequency dividing is 1.9 ps. Two chips are both applicable to 2.5 Gbit/s optical fiber communication systems.展开更多
The measures of path charge are important considerations in traffic assignment of road networks. Factors, such as travel time, fixed charge and traffic congestion which affect road users' choices of trip paths, are a...The measures of path charge are important considerations in traffic assignment of road networks. Factors, such as travel time, fixed charge and traffic congestion which affect road users' choices of trip paths, are analyzed. Travelers usually decide their trip paths based on their personal habits, preferences and the information at hand. By considering both deterministic and stochastic factors which affect the value of time (VOT) during the process of path choosing, a variational inequality model is proposed to describe the problem of traffic assignment. A lazy loading algorithm for traffic assignment is designed to solve the proposed model, and the calculation steps are given. Numerical experiment results show that compared with the all-or-nothing assignment, the proposed model and the algorithm can provide more optimal traffic assignments for road networks. The results of this study can be used to optimize traffic planning and management.展开更多
Based on the fact that the variation of tile direction of arrival (DOA) isslower than that of the channel fading, the steering vector of the desired signal is estimatedfirstly using a subspace decomposition method and...Based on the fact that the variation of tile direction of arrival (DOA) isslower than that of the channel fading, the steering vector of the desired signal is estimatedfirstly using a subspace decomposition method and then a constrained condition is configured.Traffic signals are further employed to estimate the channel vector based on the constrained leastsquares criterion. We use the iterative least squares with projection (ILSP) algorithm initializedby the pilot to get the estimation. The accuracy of channel estimation and symbol detection can beprogressively increased through the iteration procedure of the ILSP algorithm. Simulation resultsdemonstrate that the proposed algorithm improves the system performance effectively compared withthe conventional 2-D RAKE receiver.展开更多
Considering four different climate zones in China, an investigation on the choice of heat recovery ventilator for the buildings with little moisture emissions is carried out. The annual composition of energy consumpti...Considering four different climate zones in China, an investigation on the choice of heat recovery ventilator for the buildings with little moisture emissions is carried out. The annual composition of energy consumption of air intake for per unitary air ventilation flow rate is evaluated by employing the testing data of climatic parameters in eight selected cities. The analysis shows that the total heat recovery is suitable in a controlled ventilation system with air humidity controlled during heating period of all the climates. For the building without air humidity controlled in winter, the sensible heat recovery ventilators can be used in severe cold and cold regions, and total heat recovery systems are more suitable for energy saving in hot summer and cold winter and hot summer and warm winter regions.展开更多
AIM: To investigate the effect and the possible mechanism of ginsenoside Rb1 on small intestinal smooth muscle motility in mice. METHODS: Intestinal smooth muscle strips were isolated from male ICR mice (5 wk old), an...AIM: To investigate the effect and the possible mechanism of ginsenoside Rb1 on small intestinal smooth muscle motility in mice. METHODS: Intestinal smooth muscle strips were isolated from male ICR mice (5 wk old), and the effect of ginsenoside Rb1 on spontaneous contraction was recorded with an electrophysiolograph. The effect of ginsenoside Rb1 on ion channel currents, including the voltage-gated K + channel current (IK V ), calcium-activated potassium channel currents (IK Ca ), spontaneous transient outward currents and ATP-sensitive potassium channel current (IK ATP ), was recorded on freshly isolated single cells using the whole-cell patch clamp technique. RESULTS: Ginsenoside Rb1 dose-dependently inhibited the spontaneous contraction of intestinal smooth muscle by 21.15% ± 3.31%, 42.03% ± 8.23% and 67.23% ± 5.63% at concentrations of 25 μmol/L, 50 μmol/L and 100 μmol/L, respectively (n=5,P<0.05). The inhibitory effect of ginsenoside Rb1 on spontaneous contraction was significantly but incompletely blocked by 10 mmol/L tetraethylammonium or 0.5 mmol/L 4-aminopyridine, respectively (n=5, P<0.05). However, the inhibitory effect of ginsenoside Rb1 on spontaneous contraction was not affected by 10 μmol/L glibenclamide or 0.4 μmol/L tetrodotoxin. At the cell level, ginsenoside Rb1 increased outward potassium currents, and IK V was enhanced from 1137.71 ± 171.62 pA to 1449.73 ± 162.39 pA by 50 μmol/L Rb1 at +60 mV (n=6, P<0.05). Ginsenoside Rb1 increased IK Ca and enhanced the amplitudes of spontaneous transient outward currents from 582.77 ± 179.09 mV to 788.12 ± 278.34 mV (n=5, P<0.05). However, ginsenoside Rb1 (50 μmol/L) had no significant effect on IK ATP (n=3, P<0.05). CONCLUSION: These results suggest that ginsenoside Rb1 has an inhibitory effect on the spontaneous contraction of mouse intestinal smooth muscle mediated by the activation of IK V and IK Ca , but the K ATP channel was not involved in this effect.展开更多
In this paper we investigate the impact of the Atmospheric Infra-Red Sounder (AIRS) temperature retrievals on data assimilation and the resulting forecasts using the four-dimensional Local Ensemble Transform Kalman Fi...In this paper we investigate the impact of the Atmospheric Infra-Red Sounder (AIRS) temperature retrievals on data assimilation and the resulting forecasts using the four-dimensional Local Ensemble Transform Kalman Filter (LETKF) data assimilation scheme and a reduced resolution version of the NCEP Global Forecast System (GFS).Our results indicate that the AIRS temperature retrievals have a significant and consistent positive impact in the Southern Hemispheric extratropics on both analyses and forecasts,which is found not only in the temperature field but also in other variables.In tropics and the Northern Hemispheric extratropics these impacts are smaller,but are still generally positive or neutral.展开更多
Potassium transporters play crucial roles in K^+ uptake and translocation in plants. However, so far little is known about the regulatory mechanism of potassium transporters. Here, we show that a Shaker-like potassiu...Potassium transporters play crucial roles in K^+ uptake and translocation in plants. However, so far little is known about the regulatory mechanism of potassium transporters. Here, we show that a Shaker-like potassium channel AtKC1, encoded by the AtLKT1 gene cloned from the Arabidopsis thaliana low-K^+ (LK)-tolerant mutant Atlktl, significantly regulates AKTl-mediated K^+ uptake under LK conditions. Under LK conditions, the Atkcl mutants maintained their root growth, whereas wild-type plants stopped their root growth. Lesion of AtKC1 significantly enhanced the tolerance of the Atkcl mutants to LK stress and markedly increased K^+ uptake and K^+ accumulation in the Atkclmutant roots under LK conditions. Electrophysiological results showed that AtKC1 inhibited the AKT1-mediated inward K^+ currents and negatively shifted the voltage dependence of AKT1 channels. These results demonstrate that the ‘silent' K^+ channel α-subunit AtKC1 negatively regulates the AKTl-mediated K^+ uptake in Arabidopsis roots and consequently alters the ratio of root-to-shoot under LK stress conditions.展开更多
Ground-coupled heat pumps(GCHP)are commonly used in residential heating system.To mitigate the boreholes temperature dropping with operating time,a new exhaust-air recharging system is developed.The new recharging sys...Ground-coupled heat pumps(GCHP)are commonly used in residential heating system.To mitigate the boreholes temperature dropping with operating time,a new exhaust-air recharging system is developed.The new recharging system can be used in three operational modes.In this paper,a ground-coupled heat recovery ventilation(HRV)model is discussed.A thermal model is set up to find the optimal brine flow rate and heat transfer allocation ratio between exhaust and supply coils for maximum heat recovery efficiency.Contrary to the conventional liquid-loop HRV systems,the brine temperature entering the exhaust coil never goes blow zero(0℃),and hence defrosting is needless in the ground-coupled HRV system.This can make the ground-coupled HRV system over 20% more efficient than a conventional HRV system at low outdoor temperatures.展开更多
Recently, single carrier block transmission(SCBT) has received much attention in high-rate phase-coherent underwater acoustic communication.However,minimum-mean-square-error(MMSE) linear FDE may suffer performance los...Recently, single carrier block transmission(SCBT) has received much attention in high-rate phase-coherent underwater acoustic communication.However,minimum-mean-square-error(MMSE) linear FDE may suffer performance loss in the severely time dispersive underwater acoustic channel. To combat the channel distortion, a novel multi-channel receiver with maximum ratio combining and a low complex T/4 fractional iterative frequency domain equalization(FDE) is investigated to improve diversity gain and the bit error rate(BER) performance. The proposed method has been verified by the real data from a lake underwater acoustic communication test in November 2011. At 1.8 km, the useful data rates are around 1500 and 3000 bits/s for BPSK and QPSK respectively. The results show the improvements of system performance. Compared with MMSE FDE system, the output SNR improvement is 6.9 d B, and the BER is from 10-3 to no error bits for BPSK. The output SNR improvement is 5.3 d B, and the BER is from 1.91×10-2 to 2.2×10-4for QPSK.展开更多
基金The study was supported by innovation research project of Institute of Applied Ecology Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (302
文摘To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber technique, from Sept 3, 2002 to Oct 30, 2003 in two types of soil ecosystems, of which one was covered with litters on the surface soil, and the other had no litters. The results showed that litters had significant influences on CO2, N2O and CH4 fluxes (p<0.05). Their diurnal change patterns of plot with litters and litter-free plot were similar, and they all showed emission/absorption peak at 18:00. The diurnal change fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the diurnal flux of CH4 of plot with litters was lower than that of litter-free plot. The fluxes of CO2, N2O, and CH4 showed the similar seasonal patternsfor both plots. The fluxes of CO2, CH4 showed their peak fluxes in June, but the fluxes of N2O showed its peak emissions in August. The annual fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the annual flux of CH4 of plot with litters was lower than that of litter-free plot. Keywords Flux - CO2, N2O and CH4 - Seasonal variation - Diurnal variation CLC number S718.5 Document code A Foundation item: The study was supported by innovation research project of Institute of Appiied Ecology, Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (30271068)Bigraphy: XIAO Dong-mei (1979-), female, master of Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016. P. R. China.Responsible editor: Song Funan
基金The Natural Science Foundation of Hebei Province(No.E2013202228)the Science and Technology Planning Project of the Department of Transportation of Hebei Province(No.R070245)
文摘To alleviate the resulting increase in energy consumption and emissions and other issues caused by the traffic congestion ahead of the expressway toll station, a novel traffic flowcontrol method is put forward based on the environment-friendly conception. The technical thinking of inducing the slowly moving traffic into a batch pass is determined based on the conclusion of the research, traffic flowtheory and traffic sensing detection technology. The model of stop times is established and the parameters of the system are optimized in accordance with the principle of minimizing the fuel consumption. The optimal location selection of traffic control lights and Detector 2 for queue of different lengths at toll stations are calculated based on the model. Finally, the effect of the congestion flowcontrol system is verified via the Paramics simulation system. The result shows that the control system is capable of reducing90% of fuel consumption for vehicles going through toll stations.
基金Chinese Terrestrial Ecosystem Flux Observational Research Network(ChinaFlux) for providing the observational data
文摘Using the 3-year observational data from ChinaFlux (Chinese Terrestrial Ecosystem Flux Research Network), we studied the gas regulation flux dynamics and cumulative process of gas regulation value in Qianyanzhou middle subtropical plantation (QYF) and Changbai Mountain temperate mixed forest (CBF). The gas regulation service was differentiated into vegetation gas regulation service and net ecosystem gas regulation service. Carbon tax approach, reforestation cost approach and industrial oxygen approach were employed to calculate gas regulation value. Results show that there was significant seasonal variation in vegetation gas regulation flux. Daily CO2 uptake fluxes averaged 82.00 kg·ha^-·d^-1 and 59.37 kg·ha^-·d^-1 and the corresponding 02 emission fluxes were 59.65 kg·ha^-·d^-1 and 43.19 kg·ha^-·d^-1 for QYF and CBF, respectively. The cumulative curves of vegetation gas regulation value always followed a sigmoid shape, and the annual gas regulation value produced by vegetation was RMB 14342.69 yuan·ha^-1 and RMB 10384.18 yuan·ha^-1 for both QYF and CBF, respectively. In terms of monthly net ecosystem gas regulation service, QYF appeared as a CO2 sink and O2 source for the whole year, while CBF appeared to be a CO2 sink and O2 source mainly in the period between May and September. The cumulative curves of net ecosystem gas regulation value presented a sigmoid ("S") shape for QYF, while a unimodal type curve for CBF. The annual net ecosystem gas regulation value was 8470.52 yuan·ha^-1 and 5091.98yuan·ha^-1 for QYF and CBF, respectively. The economic value of both the vegetation gas regulation service and net ecosystem gas regulation service were mainly produced between May and October.
文摘The necessity and feasibility of the use of the personalized ventilation(PV)technology in a toll booth is described.First,the indoor environment of the toll booth equipped with a PV system is analyzed.Based on the analysis results,a set of equipment for controlling the indoor air quality(IAQ)of the toll booth is devised.Then,a full-scale model of the toll booth is set up in the laboratory.The airflow organization,the optimum operation parameters,and the restraint effects of the PV system on pollution are also experimentally studied.The experimental results on the air supply characteristics show that the PV system can effectively reduce the air age,improve the ventilation efficiency,and enhance the comfort and acceptability of human beings.In addition,this system plays a significant role in preventing pollution.
文摘Estimation of Rayleigh fading channels using time multiplexed pilot symbols in W CDMA (FDD) is considered. Method of interpolation on a second order curve using the instantaneous channel estimation of two contiguous slots based on least square method (SOLSM) is presented. It is demonstrated by computer simulations that the channel distortion on data symbols in a slot can be estimated effectively compared to the method using simple linear interpolation, and the performance of the Rake combiner is improved effectively especially in fast moving cases.
基金The National High Technology Research and Develop-ment Program of China (863 Program) (No.2001AA312010).
文摘2.5 Gbit/s monolithic integrated circuits (ICs) for optical fiber transmitter and receiver in 0.35 μm CMOS (complementary metal-oxide-semiconductor transistor) process are presented. The transmitter, which includes a 4: 1 multiplexer and a laser diode driver (LDD), has four 622 Mbit/s random signals as its inputs and gets a 2.5 Gbit/s driving signal as its output; the receiver detects a 2.5 Gbit/s random signal and gets four 622 Mbit/s signals at the output. The main circuits include a trans-impedance amplifier (TIA), a limiting amplifier, a clock and data recovery (CDR) unit, and a 1: 4 demultiplexer (DEMUX). Test results prove the logic functions of the transmitter to be right, and the 10% to 90% rise and fall times of transmitter's output data eye diagram are 211.1 ps and 200 ps, respectively. The sensitivity of the receiver is measured to be better than 20 mV. The root mean square jitter of the DEMUX's output data is 15.6 ps and that of the clock after 1: 4 frequency dividing is 1.9 ps. Two chips are both applicable to 2.5 Gbit/s optical fiber communication systems.
基金The National High Technology Research and Development Program of China(863 Program)(No.2007AA11Z202)the National Key Technology R&D Program of China during the 11th Five-Year Plan Period(No.2006BAJ18B03)
文摘The measures of path charge are important considerations in traffic assignment of road networks. Factors, such as travel time, fixed charge and traffic congestion which affect road users' choices of trip paths, are analyzed. Travelers usually decide their trip paths based on their personal habits, preferences and the information at hand. By considering both deterministic and stochastic factors which affect the value of time (VOT) during the process of path choosing, a variational inequality model is proposed to describe the problem of traffic assignment. A lazy loading algorithm for traffic assignment is designed to solve the proposed model, and the calculation steps are given. Numerical experiment results show that compared with the all-or-nothing assignment, the proposed model and the algorithm can provide more optimal traffic assignments for road networks. The results of this study can be used to optimize traffic planning and management.
基金The National Hi-Tech Development Plan (863-317-03-01-02-04-20).
文摘Based on the fact that the variation of tile direction of arrival (DOA) isslower than that of the channel fading, the steering vector of the desired signal is estimatedfirstly using a subspace decomposition method and then a constrained condition is configured.Traffic signals are further employed to estimate the channel vector based on the constrained leastsquares criterion. We use the iterative least squares with projection (ILSP) algorithm initializedby the pilot to get the estimation. The accuracy of channel estimation and symbol detection can beprogressively increased through the iteration procedure of the ILSP algorithm. Simulation resultsdemonstrate that the proposed algorithm improves the system performance effectively compared withthe conventional 2-D RAKE receiver.
基金National Natural Science Foundation of China(Grant No50578034)Shanghai Educational Development Foundationtitled"Shuguang Project"(Grant NO03SG30)
文摘Considering four different climate zones in China, an investigation on the choice of heat recovery ventilator for the buildings with little moisture emissions is carried out. The annual composition of energy consumption of air intake for per unitary air ventilation flow rate is evaluated by employing the testing data of climatic parameters in eight selected cities. The analysis shows that the total heat recovery is suitable in a controlled ventilation system with air humidity controlled during heating period of all the climates. For the building without air humidity controlled in winter, the sensible heat recovery ventilators can be used in severe cold and cold regions, and total heat recovery systems are more suitable for energy saving in hot summer and cold winter and hot summer and warm winter regions.
基金Supported by The National Natural Science Foundation of China, No. 30873328The State Administration of Traditional Chinese Medicine of the People’s Republic of China, No. 06-075930
文摘AIM: To investigate the effect and the possible mechanism of ginsenoside Rb1 on small intestinal smooth muscle motility in mice. METHODS: Intestinal smooth muscle strips were isolated from male ICR mice (5 wk old), and the effect of ginsenoside Rb1 on spontaneous contraction was recorded with an electrophysiolograph. The effect of ginsenoside Rb1 on ion channel currents, including the voltage-gated K + channel current (IK V ), calcium-activated potassium channel currents (IK Ca ), spontaneous transient outward currents and ATP-sensitive potassium channel current (IK ATP ), was recorded on freshly isolated single cells using the whole-cell patch clamp technique. RESULTS: Ginsenoside Rb1 dose-dependently inhibited the spontaneous contraction of intestinal smooth muscle by 21.15% ± 3.31%, 42.03% ± 8.23% and 67.23% ± 5.63% at concentrations of 25 μmol/L, 50 μmol/L and 100 μmol/L, respectively (n=5,P<0.05). The inhibitory effect of ginsenoside Rb1 on spontaneous contraction was significantly but incompletely blocked by 10 mmol/L tetraethylammonium or 0.5 mmol/L 4-aminopyridine, respectively (n=5, P<0.05). However, the inhibitory effect of ginsenoside Rb1 on spontaneous contraction was not affected by 10 μmol/L glibenclamide or 0.4 μmol/L tetrodotoxin. At the cell level, ginsenoside Rb1 increased outward potassium currents, and IK V was enhanced from 1137.71 ± 171.62 pA to 1449.73 ± 162.39 pA by 50 μmol/L Rb1 at +60 mV (n=6, P<0.05). Ginsenoside Rb1 increased IK Ca and enhanced the amplitudes of spontaneous transient outward currents from 582.77 ± 179.09 mV to 788.12 ± 278.34 mV (n=5, P<0.05). However, ginsenoside Rb1 (50 μmol/L) had no significant effect on IK ATP (n=3, P<0.05). CONCLUSION: These results suggest that ginsenoside Rb1 has an inhibitory effect on the spontaneous contraction of mouse intestinal smooth muscle mediated by the activation of IK V and IK Ca , but the K ATP channel was not involved in this effect.
基金National Natural Science Foundation of China (40975067)973 Program (2009CB421500)+1 种基金CMA Grant GYHY200806029NASA grant NNX07AM97G in U.S.A
文摘In this paper we investigate the impact of the Atmospheric Infra-Red Sounder (AIRS) temperature retrievals on data assimilation and the resulting forecasts using the four-dimensional Local Ensemble Transform Kalman Filter (LETKF) data assimilation scheme and a reduced resolution version of the NCEP Global Forecast System (GFS).Our results indicate that the AIRS temperature retrievals have a significant and consistent positive impact in the Southern Hemispheric extratropics on both analyses and forecasts,which is found not only in the temperature field but also in other variables.In tropics and the Northern Hemispheric extratropics these impacts are smaller,but are still generally positive or neutral.
基金Acknowledgments We thank Dr Emily Liman (University of Southern California, USA) for providing the pGEMHE vector for the Xenopus oocyte experiments. We also thank Dr Richer Gaber (Northwestern Uni- versity, USA) for providing the yeast mutant strain with K+ transport deficiency. We are grateful to Dr Rainer Hedrich (University of Wurzburg, Germany) for critical discussion. This work was supported by the National Natural Science Foundation of China (grant no. 30830013 to WHW), the Beijing Municipal Education Commission (grant no. YB20081001901 to WHW) and the Program of Introducing Talents of Discipline to Universities (grant no. B06003 to WHW).
文摘Potassium transporters play crucial roles in K^+ uptake and translocation in plants. However, so far little is known about the regulatory mechanism of potassium transporters. Here, we show that a Shaker-like potassium channel AtKC1, encoded by the AtLKT1 gene cloned from the Arabidopsis thaliana low-K^+ (LK)-tolerant mutant Atlktl, significantly regulates AKTl-mediated K^+ uptake under LK conditions. Under LK conditions, the Atkcl mutants maintained their root growth, whereas wild-type plants stopped their root growth. Lesion of AtKC1 significantly enhanced the tolerance of the Atkcl mutants to LK stress and markedly increased K^+ uptake and K^+ accumulation in the Atkclmutant roots under LK conditions. Electrophysiological results showed that AtKC1 inhibited the AKT1-mediated inward K^+ currents and negatively shifted the voltage dependence of AKT1 channels. These results demonstrate that the ‘silent' K^+ channel α-subunit AtKC1 negatively regulates the AKTl-mediated K^+ uptake in Arabidopsis roots and consequently alters the ratio of root-to-shoot under LK stress conditions.
文摘Ground-coupled heat pumps(GCHP)are commonly used in residential heating system.To mitigate the boreholes temperature dropping with operating time,a new exhaust-air recharging system is developed.The new recharging system can be used in three operational modes.In this paper,a ground-coupled heat recovery ventilation(HRV)model is discussed.A thermal model is set up to find the optimal brine flow rate and heat transfer allocation ratio between exhaust and supply coils for maximum heat recovery efficiency.Contrary to the conventional liquid-loop HRV systems,the brine temperature entering the exhaust coil never goes blow zero(0℃),and hence defrosting is needless in the ground-coupled HRV system.This can make the ground-coupled HRV system over 20% more efficient than a conventional HRV system at low outdoor temperatures.
基金supported in part by National Natural Science Foundation of China under Grants No.61471298 and 61101102Natural Science Basic Research Plan in Shaanxi Province of China (Program No. 2015JM6297)
文摘Recently, single carrier block transmission(SCBT) has received much attention in high-rate phase-coherent underwater acoustic communication.However,minimum-mean-square-error(MMSE) linear FDE may suffer performance loss in the severely time dispersive underwater acoustic channel. To combat the channel distortion, a novel multi-channel receiver with maximum ratio combining and a low complex T/4 fractional iterative frequency domain equalization(FDE) is investigated to improve diversity gain and the bit error rate(BER) performance. The proposed method has been verified by the real data from a lake underwater acoustic communication test in November 2011. At 1.8 km, the useful data rates are around 1500 and 3000 bits/s for BPSK and QPSK respectively. The results show the improvements of system performance. Compared with MMSE FDE system, the output SNR improvement is 6.9 d B, and the BER is from 10-3 to no error bits for BPSK. The output SNR improvement is 5.3 d B, and the BER is from 1.91×10-2 to 2.2×10-4for QPSK.