[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to ampli...[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.展开更多
基金Supported by"Provincial and Ministerial Key Discipline,Provincial Key Laboratory of University and School Laboratory Sharing Platform" ItemSouthwest Forestry University Key Fund Item(110909)~~
文摘[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.
