葡萄糖对内皮细胞蛋白激酶C活性、通透性及粘附性的影响

目的 研究不同浓度葡萄糖对血管内皮细胞的蛋白激酶C(PKC)活性、通透性及粘附性的影响。方法 用[γ32 P]ATP参入外源性底物的方法测定人脐静脉内皮细胞(HUVECs )PKC活性;用51 Cr标记的静止血小板与HUVECs保温,测定内皮细胞的粘附性;用HUVECs对白蛋白的体外通透性实验测定内皮细胞的通透性。结果 2 0mmol L的高浓度葡萄糖可最大程度活化HUVECs的PKC ,膜PKC活性为( 2 874±0 0 4 6 )mmol·mg- 1 ·min- 1 ,明显高于浆PKC活性[( 1 2 15±0 0 19)mmol·mg- 1 ·min- 1 ,P <0 0 1];HUVECs在2 0mmol L高浓度葡萄糖作用下的最大粘附率为( 6 2 84±3 2 4 ) % ,对白蛋白的最大通透率为( 0 32±0 0 0 2 )ng ml,明显高于对照组(P <0 0 1) ;HUVECs的PKC总活性与HUVECs的通透性及粘附率的相关系数r分别为0 84 6、0 734(P均<0 0 5 )。PKC抑制剂CalphostinC能抑制高浓度( 2 0mmol L)葡萄糖对HUVECs的PKC活化,并使HUVECs的通透性及粘附率明显下降。结论 高浓度葡萄糖( 2 0mmol L)可活化血管内皮细胞的PKC ,进而增加其通透性及粘附性;

含多种钙质的人工骨临床应用

含多种钙质的人工骨临床应用白求恩医科大学王庆林，曹树民吉林省人民医院刘利口腔颌面部畸形的修复是口腔颌面外科中一个重要内容。而颌面部功能的重建和形态恢复，在很大程度上取决于该部位骨组织正常支架的建立。对如何修复骨缺损以及畸形，目前所应用的无论是生物类材...

Numerical simulation and experiment analysis of improving permeability by deep-hole presplitting explosion in high gassy and low permeability coal seam

Created a new damage model for explosive for LS-DYNA3D,taking advantageof the Taylor method aimed at the high gassy and low permeability coal seam,and numericallysimulated and analyzed the deep-hole presplitting explosion.The entire processof explosion was represented,including cracks caused by dynamic pressure,transmissionand vibration superposition of stress waves,as well as cracks growth driven by gas generatedby explosion.The influence of the cracks generated in the process of explosion andthe performance of improving permeability caused by the difference of interval between.explosive holes were analyzed.A reasonable interval between explosive holes of deepholepresplitting explosions in high gassy and low permeability coal seams was proposed,and the resolution of gas drainage in high gassy and low permeability coal seam was putforward.