Objective: to explore a new serological method for detecting Helicobacter pylori ( H. pylori ) infection. Methods: Serum soluble antigen of H. pylori was detected by using avidin-biotin ELISA technique to evaluate t...Objective: to explore a new serological method for detecting Helicobacter pylori ( H. pylori ) infection. Methods: Serum soluble antigen of H. pylori was detected by using avidin-biotin ELISA technique to evaluate the status of H. pylori infection and for comparison with rapid urease test ( RUT ), histologic examination and serology. Results: The sensitivity, specificity, positive predictive value and negative predictive value were 77.46%, 91.07%, 91.67% and 76.12 %, respectively. The prevalence rate of serum H. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by 14C-UBT methods ( P> 0.05 ). Conclusions: The detection of serum H. pylori soluble antigen(HpSAg) could be used as a new serological method which is accurate, and convenient, not affected by the memorizing reaction of serum antibody; is more sensitive, more specific and suitable for clinical diagnosis, and evaluation of eradication and for follow-up of H. pylori as well as for detection in children and pregnant women.展开更多
文摘Objective: to explore a new serological method for detecting Helicobacter pylori ( H. pylori ) infection. Methods: Serum soluble antigen of H. pylori was detected by using avidin-biotin ELISA technique to evaluate the status of H. pylori infection and for comparison with rapid urease test ( RUT ), histologic examination and serology. Results: The sensitivity, specificity, positive predictive value and negative predictive value were 77.46%, 91.07%, 91.67% and 76.12 %, respectively. The prevalence rate of serum H. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by 14C-UBT methods ( P> 0.05 ). Conclusions: The detection of serum H. pylori soluble antigen(HpSAg) could be used as a new serological method which is accurate, and convenient, not affected by the memorizing reaction of serum antibody; is more sensitive, more specific and suitable for clinical diagnosis, and evaluation of eradication and for follow-up of H. pylori as well as for detection in children and pregnant women.
