A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer) .Firstly, a standard t...A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer) .Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4- 10.4 conidia/mm^2) of Z. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, bioassay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower of Z. anhuiensis conidia at each of four dosages (resulting from exposures of 0.3 - 8.0 min) . Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1 - 12 h after exposure under temperature treatments of 15 and 20℃, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection of M. persicae from Z. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20℃ before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤ 1 h to infect > 50% of the aphids at a dosage of > 1.5 conida/mm^2 and > 90% at > 50 conidia/mm^2 .展开更多
Fire is an important ecological factor and what constitutes appropriate fire management is much debated in the US and elsewhere. The role of fire as a source of greenhouse gases has been intensively investigated, but ...Fire is an important ecological factor and what constitutes appropriate fire management is much debated in the US and elsewhere. The role of fire as a source of greenhouse gases has been intensively investigated, but less is known about the production rate of the solid black carbon residue from fires. Black carbon accumulates in soil as it has longer turnover times than plant residues. To understand the significance of black carbon production during wildfire, we quantified black carbon using hydropyrolysis in O and A horizons before and after a prescribed bum at four sites in the New Jersey Pine Barrens forest in the North-Eastern US. Black carbon was found in both O- and A-horizons at all investigated sites, stocks in the range of 61.31-168.15 g m^-2 in the O-horizon and 169.59-425.25 g m^-2 in the A-horizon. Total black carbon stocks did not increase following the fire suggesting that either black carbon production in fires may be small compared to the variability, or that equivalent amounts of black carbon formed in previous fires may have been consumed in the fire. The study raises questions about how black carbon production and consumption in ftres can be quantified separately.展开更多
文摘A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer) .Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4- 10.4 conidia/mm^2) of Z. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, bioassay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower of Z. anhuiensis conidia at each of four dosages (resulting from exposures of 0.3 - 8.0 min) . Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1 - 12 h after exposure under temperature treatments of 15 and 20℃, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection of M. persicae from Z. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20℃ before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤ 1 h to infect > 50% of the aphids at a dosage of > 1.5 conida/mm^2 and > 90% at > 50 conidia/mm^2 .
文摘Fire is an important ecological factor and what constitutes appropriate fire management is much debated in the US and elsewhere. The role of fire as a source of greenhouse gases has been intensively investigated, but less is known about the production rate of the solid black carbon residue from fires. Black carbon accumulates in soil as it has longer turnover times than plant residues. To understand the significance of black carbon production during wildfire, we quantified black carbon using hydropyrolysis in O and A horizons before and after a prescribed bum at four sites in the New Jersey Pine Barrens forest in the North-Eastern US. Black carbon was found in both O- and A-horizons at all investigated sites, stocks in the range of 61.31-168.15 g m^-2 in the O-horizon and 169.59-425.25 g m^-2 in the A-horizon. Total black carbon stocks did not increase following the fire suggesting that either black carbon production in fires may be small compared to the variability, or that equivalent amounts of black carbon formed in previous fires may have been consumed in the fire. The study raises questions about how black carbon production and consumption in ftres can be quantified separately.
