The anthropogenic managements of forest have created a network of roads resulting in the loss and alternation of habitat. To better understand road′s impact on animal habitats, we assessed the habitat pattern of sabl...The anthropogenic managements of forest have created a network of roads resulting in the loss and alternation of habitat. To better understand road′s impact on animal habitats, we assessed the habitat pattern of sables(Martes zibellina), one of rodents within national first-class protected species, when roads are considered in Huzhong area in Da Hinggan Mountains, northeastern China. Employing published literatures about behavior ecology, aerial photographs and forest stand maps, we classified the study area into three habitat types including best-suitable, suitable and unsuitable habitats based on sable habitat requirements at the landscape scale including four variables derived from forest source map with attribute database. Results indicated the loss and significant fragmentation of best-suitable habitat and home range habitat when roads, especially 150 m avoidance distance of roads, were considered. The roads reduced and fragmented highly suitable habitats more significantly during earlier development period than the later development period. Additionally, the suitable area percentage increased with increasing distance to roads. This study helped to identify the suitable area for sables and location of sable population. Also, this study suggested the passage construction and road management involving road closure and removal will reduce the fragmentation functionally and benefit the sable population.展开更多
AIM： To assess the role of hyperpolarization-activated cyclic nucleotide-gated cation （HCN） channels in regu- lating the excitability of vagal and spinal gut afferents. METHODS： The mechanosensory response of mese...AIM： To assess the role of hyperpolarization-activated cyclic nucleotide-gated cation （HCN） channels in regu- lating the excitability of vagal and spinal gut afferents. METHODS： The mechanosensory response of mesen- teric afferent activity was measured in an ex vivo murine jejunum preparation. HCN channel activity was recorded through voltage and current clamp in acutely dissoci- ated dorsal root ganglia （DRG） and nodose ganglia （NG） neurons retrogradely labeled from the small intestine through injection of a fluorescent marker （DiI）. The isoforms of HCN channels expressed in DRG and NG neurons were examined by immunohistochemistry. RESULTS： Ramp distension of the small intestine evok- ed biphasic increases in the afferent nerve activity, re- flecting the activation of low- and high-threshold fibers.HCN blocker CsCl （5 mmol/L） preferentially inhibited the responses of low-threshold fibers to distension and showed no significant effects on the high-threshold re- sponses. The effect of CsCI was mimicked by the more selective HCN blocker ZD7288 （10 ~mol/L）. In 71.4% of DiI labeled DRG neurons （/7 = 20） and 90.9% of DiI labeled NG neurons （n = 10）, an inward current （Ih current） was evoked by hyperpolarization pulses which was fully eliminated by extracellular CsCI. In neurons expressing Ih current, a typical ＂sag＂ was observed upon injection of hyperpolarizing current pulses in cur- rent-clamp recordings. CsCI abolished the sag entirely. In some DiI labeled DRG neurons, the Ih current was potentiated by 8-Br-cAMP, which had no effect on the Ih current of DiI labeled NG neurons. Immunohistochem- istry revealed differential expression of HCN isoforms in vagal and spinal afferents, and HCN2 and HCN3 seemed to be the dominant isoform in DRG and NG, respec- tively.CONCLUSION： HCNs differentially regulate the excit- ability of vagal and spinal afferent of murine small in- testine.展开更多
Early Renaissance man regained the independence of individual personality, but by the fear of God into the evolution away from God, from the backwash toward the loss. Late Renaissance reflection and adjustments is com...Early Renaissance man regained the independence of individual personality, but by the fear of God into the evolution away from God, from the backwash toward the loss. Late Renaissance reflection and adjustments is comforting one, rational constraints original desire, divinity give directions to humanity, which is the most profound meaning of humanism. For human freedom and liberation, post-humanism made a valuable exploration, by absorbing humanistic ingredients of the ancient Greek - Roman and Hebrew - Christian culture, tried to avoid walking single dimension of cultural evolution orientation.展开更多
AIM: To investigate whether the combined methods of unilateral thyroparathyroidectomy (TPX) and subdiaphragmatic vagotomy (VAX) can be adapted for rats and used as a reliable method to produce a rat model of long-term...AIM: To investigate whether the combined methods of unilateral thyroparathyroidectomy (TPX) and subdiaphragmatic vagotomy (VAX) can be adapted for rats and used as a reliable method to produce a rat model of long-term reduction of gastrointestinal (GI) motor function. METHODS: Male Sprague-Dawley rats were randomly divided into 3 groups, normal, sham-operated and unilateral TPX plus VAX. The TPX plus VAX rats received VAX 7 d after application of TPX, and dietary intake and fecal output were then measured daily for 1 wk.After completion of the experiments, gastric emptying and small bowel transit were measured in vivo, and the contractile responses of colonic strips to excitatory and inhibitory neurotransmitters were estimated using isometric force transducers in vitro. RESULTS: In comparison with normal and sham-operated rats, rats which received unilateral TPX plus VAX showed a significant decrease in body weight and in fecal pellet number and weight throughout the entire week. Application of TPX plus VAX to rats markedly delayed gastric emptying and small bowel transit. In TPX plus VAX rats, the longitudinal muscles of the proximal colon showed a significant reduction in contractile responses to acetylcholine (5 × 10-6 mol/L), and a dramatic attenuation of contractile responses was also observed in both the longitudinal and circular muscles of the distal colon. However, the spontaneous contractility of the colonic strips from TPX plus VAX rats was not significantly affected by treatment with N-nitro-Larginine-methyl ester (0.1 mol/L). CONCLUSION: The results indicate that unilateral TPX plus VAX reduced the motor function of the GI tract in rats, and the reduced gut motility is likely mediated, at least in part, by inhibition of the excitatory neurotransmitter system.展开更多
There is the question--can we hope that humanism and erudition can redevelop nowadays？ In the long history of humanistic inheritance, from ancient times till the end of the 18th century, there was obvious decadence o...There is the question--can we hope that humanism and erudition can redevelop nowadays？ In the long history of humanistic inheritance, from ancient times till the end of the 18th century, there was obvious decadence of essential humanistic points. As the historial period, humanism means revival of classical culture, and as a specific culturological phenomenon, it means erudite education created by Hellens and developed by Romans, who recovered ＂humanitas＂ as global education. Italy was the first who helped humanists to contemplate on classical culture; and in post-medieval age, Italian humanists achieved a supreme measure of humanism through their ＂humanistic studies.＂ We will try to point out good achievements of humanism erudite education in European culture in later epochs. Humanism leads man to turn over to world--after medieval stricture--and it becomes self-conscious revolution, what we can see in Latin literature till the 18th century. Rise of individualism, development of libraries and humanistic academies give chance to latin tradition in European literature as universal discipline, including erudite education in international corpus （Ragusa also）. High-intellectual and latinized elites with humanistic state of mind develop complex spiritual inteligency with science requests in later epochs too. We will try to explain in which degree humanism, with its synthetic point of view, influences on later corpus of European literary themes and how great its actuallity is nowdays.展开更多
基金Under the auspices of National Natural Science Foundation of China(No.41271201,31070422,41201185)
文摘The anthropogenic managements of forest have created a network of roads resulting in the loss and alternation of habitat. To better understand road′s impact on animal habitats, we assessed the habitat pattern of sables(Martes zibellina), one of rodents within national first-class protected species, when roads are considered in Huzhong area in Da Hinggan Mountains, northeastern China. Employing published literatures about behavior ecology, aerial photographs and forest stand maps, we classified the study area into three habitat types including best-suitable, suitable and unsuitable habitats based on sable habitat requirements at the landscape scale including four variables derived from forest source map with attribute database. Results indicated the loss and significant fragmentation of best-suitable habitat and home range habitat when roads, especially 150 m avoidance distance of roads, were considered. The roads reduced and fragmented highly suitable habitats more significantly during earlier development period than the later development period. Additionally, the suitable area percentage increased with increasing distance to roads. This study helped to identify the suitable area for sables and location of sable population. Also, this study suggested the passage construction and road management involving road closure and removal will reduce the fragmentation functionally and benefit the sable population.
基金Supported by Science and Technology Commission of Shanghai Municipality,No. 10ZR1417300Educational Commission of Shanghai Municipality,No. 10ZZ69
文摘AIM： To assess the role of hyperpolarization-activated cyclic nucleotide-gated cation （HCN） channels in regu- lating the excitability of vagal and spinal gut afferents. METHODS： The mechanosensory response of mesen- teric afferent activity was measured in an ex vivo murine jejunum preparation. HCN channel activity was recorded through voltage and current clamp in acutely dissoci- ated dorsal root ganglia （DRG） and nodose ganglia （NG） neurons retrogradely labeled from the small intestine through injection of a fluorescent marker （DiI）. The isoforms of HCN channels expressed in DRG and NG neurons were examined by immunohistochemistry. RESULTS： Ramp distension of the small intestine evok- ed biphasic increases in the afferent nerve activity, re- flecting the activation of low- and high-threshold fibers.HCN blocker CsCl （5 mmol/L） preferentially inhibited the responses of low-threshold fibers to distension and showed no significant effects on the high-threshold re- sponses. The effect of CsCI was mimicked by the more selective HCN blocker ZD7288 （10 ~mol/L）. In 71.4% of DiI labeled DRG neurons （/7 = 20） and 90.9% of DiI labeled NG neurons （n = 10）, an inward current （Ih current） was evoked by hyperpolarization pulses which was fully eliminated by extracellular CsCI. In neurons expressing Ih current, a typical ＂sag＂ was observed upon injection of hyperpolarizing current pulses in cur- rent-clamp recordings. CsCI abolished the sag entirely. In some DiI labeled DRG neurons, the Ih current was potentiated by 8-Br-cAMP, which had no effect on the Ih current of DiI labeled NG neurons. Immunohistochem- istry revealed differential expression of HCN isoforms in vagal and spinal afferents, and HCN2 and HCN3 seemed to be the dominant isoform in DRG and NG, respec- tively.CONCLUSION： HCNs differentially regulate the excit- ability of vagal and spinal afferent of murine small in- testine.
文摘Early Renaissance man regained the independence of individual personality, but by the fear of God into the evolution away from God, from the backwash toward the loss. Late Renaissance reflection and adjustments is comforting one, rational constraints original desire, divinity give directions to humanity, which is the most profound meaning of humanism. For human freedom and liberation, post-humanism made a valuable exploration, by absorbing humanistic ingredients of the ancient Greek - Roman and Hebrew - Christian culture, tried to avoid walking single dimension of cultural evolution orientation.
基金Grants of the Korea Healthcare Technology Rand D Project, Ministry of Health, Welfare and Family AffairsA090216the National Research Foundation of Korea2011-0014777
文摘AIM: To investigate whether the combined methods of unilateral thyroparathyroidectomy (TPX) and subdiaphragmatic vagotomy (VAX) can be adapted for rats and used as a reliable method to produce a rat model of long-term reduction of gastrointestinal (GI) motor function. METHODS: Male Sprague-Dawley rats were randomly divided into 3 groups, normal, sham-operated and unilateral TPX plus VAX. The TPX plus VAX rats received VAX 7 d after application of TPX, and dietary intake and fecal output were then measured daily for 1 wk.After completion of the experiments, gastric emptying and small bowel transit were measured in vivo, and the contractile responses of colonic strips to excitatory and inhibitory neurotransmitters were estimated using isometric force transducers in vitro. RESULTS: In comparison with normal and sham-operated rats, rats which received unilateral TPX plus VAX showed a significant decrease in body weight and in fecal pellet number and weight throughout the entire week. Application of TPX plus VAX to rats markedly delayed gastric emptying and small bowel transit. In TPX plus VAX rats, the longitudinal muscles of the proximal colon showed a significant reduction in contractile responses to acetylcholine (5 × 10-6 mol/L), and a dramatic attenuation of contractile responses was also observed in both the longitudinal and circular muscles of the distal colon. However, the spontaneous contractility of the colonic strips from TPX plus VAX rats was not significantly affected by treatment with N-nitro-Larginine-methyl ester (0.1 mol/L). CONCLUSION: The results indicate that unilateral TPX plus VAX reduced the motor function of the GI tract in rats, and the reduced gut motility is likely mediated, at least in part, by inhibition of the excitatory neurotransmitter system.
文摘There is the question--can we hope that humanism and erudition can redevelop nowadays？ In the long history of humanistic inheritance, from ancient times till the end of the 18th century, there was obvious decadence of essential humanistic points. As the historial period, humanism means revival of classical culture, and as a specific culturological phenomenon, it means erudite education created by Hellens and developed by Romans, who recovered ＂humanitas＂ as global education. Italy was the first who helped humanists to contemplate on classical culture; and in post-medieval age, Italian humanists achieved a supreme measure of humanism through their ＂humanistic studies.＂ We will try to point out good achievements of humanism erudite education in European culture in later epochs. Humanism leads man to turn over to world--after medieval stricture--and it becomes self-conscious revolution, what we can see in Latin literature till the 18th century. Rise of individualism, development of libraries and humanistic academies give chance to latin tradition in European literature as universal discipline, including erudite education in international corpus （Ragusa also）. High-intellectual and latinized elites with humanistic state of mind develop complex spiritual inteligency with science requests in later epochs too. We will try to explain in which degree humanism, with its synthetic point of view, influences on later corpus of European literary themes and how great its actuallity is nowdays.
