For orthogonal frequency division multiplexing (OFDM) wireless communication, the system throughput and data rate are usually limited by pilots, especially in a high mobility environment. In this paper, an enhanced it...For orthogonal frequency division multiplexing (OFDM) wireless communication, the system throughput and data rate are usually limited by pilots, especially in a high mobility environment. In this paper, an enhanced iterative joint channel estimation and symbol detection algorithm is proposed to enhance the system throughput and data rate. With lower pilot power, the proposed scheme increases system throughput firstly, and then the channel estimation and symbol detection proceed iteratively within one OFDM symbol to improve the BER performance. In the proposed algorithm, the original channel estimate of each OFDM symbol is based on the channel estimate of the previous OFDM symbol, thus the variation of the mobile channel is traced efficiently, so the number of pilots in the time domain can be reduced greatly. Besides reducing the system overhead, the proposed algorithm is also shown by simulation to give much better BER performance than the conventional iterative algorithm does.展开更多
The module for function electrical stimulation (FES) of neurons is designed for the research of the neural function regeneration microelectronic system, which is an in-body embedded micro module. It is implemented b...The module for function electrical stimulation (FES) of neurons is designed for the research of the neural function regeneration microelectronic system, which is an in-body embedded micro module. It is implemented by using discrete devices at first and characterized in vitro. The module is used to stimulate sciatic nerve and spinal cord of rats and rabbits for in-vivo real-time experiments of the neural function regeneration system. Based on the module, a four channel module for the FES of neurons is designed for 12 sites cuff electrode or 10 sites shaft electrode. Three animal experiments with total five rats and two rabbits were made. In the in-vivo experiment, the neural signals including spontaneous and imitated were regenerated by the module. The stimulating signal was used to drive sciatic nerve and spinal cord of rats and rabbits, successfully caused them twitch in different parts of their bodies, such as legs, tails, and fingers. This testifies that the neural function regeneration system can regenerate the neural signals.展开更多
Finding channel sandbodies is an important task in oil and gas exploration due to the importance of fluvial reservoirs. It is difficult to describe fluvial reservoirs in detail owing to their frequent changes and seri...Finding channel sandbodies is an important task in oil and gas exploration due to the importance of fluvial reservoirs. It is difficult to describe fluvial reservoirs in detail owing to their frequent changes and serious intersections, as well as limitations of S/N ratio and seismic data resolution. Based on the Laohekou 3D data in Shengli Oilfield, we analyze the general characteristics of fluvial reservoirs in this area, from which we find that they are characterized by strong amplitudes on seismic profiles, high continuity on time slices, and low frequency in the frequency domain. In addition, a cluster of strong string-bead- like reflections was found after color processing and detailed interpretation. To understand this observation, we conduct forward modeling to explain the mechanism. This provides a new way to identify ancient channels in similar areas. By using the multi-attribute fusion and RGB display techniques, channel incision is more obvious and the characteristics of the channel structures are manifested much better. Finally, we introduce and apply multi-wavelet detection technology to identify weaker fluvial reservoir signals.展开更多
Aim To get the theory base of designing FM fuze's jamming signal, its jamming mechanism was studied. Methods A sinusoidal FM fuze was analyzed in time domain and frequency domain and the concept of channel lea...Aim To get the theory base of designing FM fuze's jamming signal, its jamming mechanism was studied. Methods A sinusoidal FM fuze was analyzed in time domain and frequency domain and the concept of channel leak was presented. Results It was proved that information channel leak exists in FM fuze because of the nonlinear property of the mixer. The jamming signal was designed based on the channel leak and the jamming mechanism was analyzed in detail. Conclusion This kind of jamming signal can jam the sinusoidal FM fuzes effectively just depending on the jamming signal's feature itself. It's different from the traditional jamming way of simulating echo. Though the sinusoidal FM fuze was just analyzed, the principle is applicable to all FM fuzes. At the same time, it may be used as the reference for FM radar and communication countermeasures.展开更多
Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA...Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA) in Vicia faba. Stomatal opening was completely inhibited by external application of 1 μmol/L ABA, and such ABA inhibition was significantly reversed by the addition of CDPK inhibitor trifluoperazine (TFP). The inward whole cell K + currents were inhibited by 60% in the presence of 1 μmol/L intracellular ABA, and this inhibition was completely abolished by the addition of CDPK competitive substrate histone Ⅲ S. The results suggest that CDPKs may be involved in the signal transduction cascades of ABA regulated stomatal movements.展开更多
Objective To investigate effect of pinacidil, an ATP sensitive potassium channel (KATP) opener, on the neuronal apoptosis and its signaling transduction mechanism following focal cerebral ischemia-reperfusion in rat...Objective To investigate effect of pinacidil, an ATP sensitive potassium channel (KATP) opener, on the neuronal apoptosis and its signaling transduction mechanism following focal cerebral ischemia-reperfusion in rats. Methods One hundred male Wistar rats were randomly divided into four groups: A, sham-operated group; B, ischemia-reperfusion group; C, KATe opener treatment group; and D, KATe opener and blocker treatment group. The middle cerebral artery occlusion (MCAO) model was established by using the intraluminal suture occlusion method, neuronal apoptosis was determined by TUNEL staining, and expressions of caspase-8, caspase-9 and caspase-3 mRNA were detected by in situ hybridization. Results (1) The numbers of apoptotic neurons at 12 h, 24 h, 48 h, and 72 h were significantly less in group C than in groups B and D (P 〈 0.01 or P 〈 0.05); and there was no difference between groups B and D at all time points (P 〉 0.05). (2) The expressions of caspase-3 mRNA and caspase-8 mRNA at all times and the expressions of caspase-9 mRNA at 12 h, 24 h, 48 h, 72 h were significantly lower in group C than in groups B and D (P 〈 0.01 or P 〈 0.05); and there were no differences between groups B and D at all time points (P 〉 0.05). Conclusions KATP opener can significantly decrease the neuronal apoptosis and the expressions of caspase-3, caspase-8 and caspase-9 mRNAs following cerebral ischemiareperfusion. The neuronal apoptosis may be decreased by the inhibition of both mitochondrial and death-receptor signal pathways.展开更多
A novel secret key generation(SKG)method based on two-way randomness is proposed for TDD-SISO system.The legitimate transceivers mutually transmit their own random signal via reciprocal wireless channel,then the multi...A novel secret key generation(SKG)method based on two-way randomness is proposed for TDD-SISO system.The legitimate transceivers mutually transmit their own random signal via reciprocal wireless channel,then the multiplication of transmitted and received signal is used as common randomness to generate secret keys.In quasi-static channel,the theoretical SKG rates(SKGRs)of the three SKG methods,namely wireless channel based,one-way randomness and two-way randomness,are derived and compared.Further,two practical SKG schemes based on twoway randomness,Scheme-1bit and Scheme-3bit,are completely designed and simulated.Generally,Scheme-1bit applies to low signal to noise ratio(SNR)scenarios and achieves 0.13~0.86bit/T_s SKGR and 10^(-2)~10^(-5) level secret key outage probability(SKOP),while Scheme-3bit fits high SNR situation and obtains 0.93~1.35bit/T_s SKGR and 10^(-3)~10^(-4) level SKOP.At last,the national institute of standards and technology(NIST)test is conducted to evaluate the secret key randomness(SKRD)and the test results show that both of the proposed schemes have passed the test.展开更多
In order to improve the operational efficiency of heavy left-turn demand intersections,an optimal allocation model of an intersection with dynamic use of exit lanes for left turns(EFL)is proposed.The constraints of se...In order to improve the operational efficiency of heavy left-turn demand intersections,an optimal allocation model of an intersection with dynamic use of exit lanes for left turns(EFL)is proposed.The constraints of setting EFL are analyzed,including the number and length of reverse variable lanes,flow direction constraints,and signal constraints,etc.The constraints and control variables are combined in a unified framework for simultaneous optimization.The objective functions are defined as the average delay and left-turn capacity of an intersection.The model is solved by a non-dominated genetic algorithm(NSGA-Ⅱ).The results show that after the optimal allocation of EFL,the average vehicle delays of the intersection can be reduced by 14.9%and left-turn capacity can be increased by 19.3%.The effectiveness of the optimal allocation model of EFL is demonstrated.展开更多
AIM: To develop a simplified and efficient method for the preparation of hepatitis C virus (HCV) cDNA microarray probes.METHODS: With the technique of restriction display PCR (RD-PCR), restriction enzyme Sau3A I was c...AIM: To develop a simplified and efficient method for the preparation of hepatitis C virus (HCV) cDNA microarray probes.METHODS: With the technique of restriction display PCR (RD-PCR), restriction enzyme Sau3A I was chosen to digest the full-length HCV cDNAs. The products were classified and re-amplified by RD-PCR. We separated the differential genes by polyacrylamide gel electrophoresis and silver staining. Single bands cut out from the polyacrylamide gel were isolated. The third-round PCR was performed using the single bands as PCR template.The RD-PCR fragments were purified and cloned into the pMD18-T vector. The recombinant plasmids were extracted from positive clones, and the target gene fragments were sequenced. The cDNA microarray was prepared by spotting RD-PCR products to the surface of amino-modified glass slides using a robot. We validated the detection of microarray by hybridization and sequence analysis.RESULTS: A total of 24 different cDNA fragments ranging from 200 to 800 bp were isolated and sequenced,which were the specific gene fragments of HCV. These fragments could be further used as probes in microarray preparation. The diagnostic capability of the microarray was evaluated after the washing and scanning steps. The results of hybridization and sequence analysis showed that the specificity, sensitivity, accuracy, reproducibility,and linearity in detecting HCV RNA were satisfactory.CONCLUSION: The RD-PCR technique is of great value in obtaining a large number of size-comparable gene probes, which provides a speedy protocol in generating probes for the preparation of microarrays. Microarray prepared as such could be further optimized and applied in the clinical diagnosis of HCV.展开更多
There is evidence to suggest that follicle-stimulating hormone (FSH) can facilitate the neovascularization of ovarian cancers by increasing vascular endothelial growth factor (VEGF) expression in cancer cells, alt...There is evidence to suggest that follicle-stimulating hormone (FSH) can facilitate the neovascularization of ovarian cancers by increasing vascular endothelial growth factor (VEGF) expression in cancer cells, although the underlying molecular mechanism of this process is not well known. Therefore, we investigated the effect of FSH on VEGF expression in the ovarian cancer cell lines SKOV-3 and ES-2. Treatment with FSH significantly increased VEGF expression in a dose- and time-dependent manner. In addition, FSH treatment enhanced the expression of survivin and hypoxlainducible factor-1 (HIF-1α). Knockdown of survivin or HIF-1α suppressed VEGF expression, but only knockdown of survivin inhibited FSH-stimulated VEGF expression. Pretreatment with LY294002, a phosphoinositide 3-kinase (PI3K)/AKT inhibitor, neutralized the enhanced expression of survivin induced by FSH, but treatment with U0126, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor, had no such effect. We further showed that ovarian serous cystadenocarcinoma samples had much higher incidence of positive AKT and phosphorylated AKT (pAKT) protein staining than did benign ovarian cystadenoma samples (p 〈 0.01). The 5-year survival rate was only about 15% in patients with ovarian serous cystadenocarcinoma who had AKT and pAKT expression, whereas it was about 80% in those who did not have AKT or pAKT expression. Taken together, these results indicate that FSH increases the expression of VEGF by upregulating the expression of survivin, which is activated by the PI3K/AKT signaling pathway. Understanding the role of the PI3K/AKT pathway in FSH-stimulated expression of survivin and VEGF will be beneficial for evaluating the prognosis for patients with ovarian serous cystadenocarcinoma and for pursulug effective treatment against this disease.展开更多
L (2, 1)-labeling number, λ(G( Z , D)) , of distance graph G( Z , D) is studied. For general finite distance set D , it is shown that 2D+2≤λ(G( Z , D))≤D 2+3D. Furthermore, λ(G( Z , D)) ≤8 when...L (2, 1)-labeling number, λ(G( Z , D)) , of distance graph G( Z , D) is studied. For general finite distance set D , it is shown that 2D+2≤λ(G( Z , D))≤D 2+3D. Furthermore, λ(G( Z , D)) ≤8 when D consists of two prime positive odd integers is proved. Finally, a new concept to study the upper bounds of λ(G) for some special D is introduced. For these sets, the upper bound is improved to 7.展开更多
The intelligent pig based on the (MFL) is frequently used for in-line inspection of transportation pipelines. The article discusses the key technology of an MFL tool that includes the sensors structure, the constituti...The intelligent pig based on the (MFL) is frequently used for in-line inspection of transportation pipelines. The article discusses the key technology of an MFL tool that includes the sensors structure, the constitution of tool hardware, software and the analysis method of MFL signal.展开更多
Signal-to-noise ratio (SNR) and channel estimations are critical for 60-GHz communications to track the optimal trans- mission and reception beam pairs. However, the excessive pilot overhead for the estima- tions se...Signal-to-noise ratio (SNR) and channel estimations are critical for 60-GHz communications to track the optimal trans- mission and reception beam pairs. However, the excessive pilot overhead for the estima- tions severely reduces system throughput in fast-rotation scenarios. In order to address this problem, we firstly demonstrate the potential sparseness property of 60-GHz channel in beam tracking; subsequently, via exploiting this property, we propose a novel compressed SNR-and-channel estimation. The estimation is conducted in a three-stage fashion, includ- ing the unstructured estimation, nonzero-tap detection, and structured estimation with non- zero-tap location. Numerical simulations show that, in the case of substantial reduction of the pilot overhead, the proposed estimator still reveals a significant improvement in terms of estimation performance over the scheme in IEEE 802.1 lad. Furthermore, it is also demon- strated that the proposed SNR and channel estimators can approach the lower bounds in sparse channels so long as SNR exceeds 8 dB.展开更多
To reduce the delay of left-turning buses and improve the traffic efficiency at signalized intersections,a novel variable bus approach lane(VBAL)control method based on bus pre-signals is proposed.This method combines...To reduce the delay of left-turning buses and improve the traffic efficiency at signalized intersections,a novel variable bus approach lane(VBAL)control method based on bus pre-signals is proposed.This method combines the variable lane with the bus priority pre-signal,and realizes the left-turning bus priority without causing great impact on other vehicles.To validate the effectiveness of the method,the VBAL scheme was compared with the single left-turn lane scheme(SLTL)and the double left-turn lane scheme(DLTL).On this basis,the delay change calculation model of left-turning buses and through vehicles were established by the cumulative curve graphic method.The influence of vehicle proportion and green split on the model was studied through sensitivity analysis.The results show that VBAL can reduce the delay of left-turning bus and the increase of through vehicle delay to the greatest extent.Finally,the scheme was applied to a real-world intersection,and the results demonstrate the effectiveness and advantage of the VBAL scheme.展开更多
For a graph G and two positive integers j and k an m-L j k -edge-labeling of G is an assignment from the set 0 1 … m-to the edges such that adjacent edges receive labels that differ by at least j and edges at distanc...For a graph G and two positive integers j and k an m-L j k -edge-labeling of G is an assignment from the set 0 1 … m-to the edges such that adjacent edges receive labels that differ by at least j and edges at distance two receive labels that differ by at least k.Theλ′j k-number of G denoted byλ′j k G is the minimum integer m overall m-L j k -edge-labeling of G.The necklace is a specific type of Halin graph.The L 1 2 -edge-labeling of necklaces is studied and the lower and upper bounds on λ′1 2-number for necklaces are given.Also both the lower and upper bounds are attainable.展开更多
The properties of the paths in an ROBDD representation of a Boolean function are presented and proved in the present paper, and the applications of ROBDD in calculating signal probability are also discussed. By this m...The properties of the paths in an ROBDD representation of a Boolean function are presented and proved in the present paper, and the applications of ROBDD in calculating signal probability are also discussed. By this method, the troublesome calculation of the correlation among the nodes, which is caused by the re-convergent fan-out in digital system, can be avoided and power estimation can be faster than simulation-based method in [1].展开更多
文摘For orthogonal frequency division multiplexing (OFDM) wireless communication, the system throughput and data rate are usually limited by pilots, especially in a high mobility environment. In this paper, an enhanced iterative joint channel estimation and symbol detection algorithm is proposed to enhance the system throughput and data rate. With lower pilot power, the proposed scheme increases system throughput firstly, and then the channel estimation and symbol detection proceed iteratively within one OFDM symbol to improve the BER performance. In the proposed algorithm, the original channel estimate of each OFDM symbol is based on the channel estimate of the previous OFDM symbol, thus the variation of the mobile channel is traced efficiently, so the number of pilots in the time domain can be reduced greatly. Besides reducing the system overhead, the proposed algorithm is also shown by simulation to give much better BER performance than the conventional iterative algorithm does.
基金The National Natural Science Foundation of China(No69825101,90377013)
文摘The module for function electrical stimulation (FES) of neurons is designed for the research of the neural function regeneration microelectronic system, which is an in-body embedded micro module. It is implemented by using discrete devices at first and characterized in vitro. The module is used to stimulate sciatic nerve and spinal cord of rats and rabbits for in-vivo real-time experiments of the neural function regeneration system. Based on the module, a four channel module for the FES of neurons is designed for 12 sites cuff electrode or 10 sites shaft electrode. Three animal experiments with total five rats and two rabbits were made. In the in-vivo experiment, the neural signals including spontaneous and imitated were regenerated by the module. The stimulating signal was used to drive sciatic nerve and spinal cord of rats and rabbits, successfully caused them twitch in different parts of their bodies, such as legs, tails, and fingers. This testifies that the neural function regeneration system can regenerate the neural signals.
基金sponsored by The Science and Technology Research Project,Shengli Oilfield (Grant No. YKW1002)
文摘Finding channel sandbodies is an important task in oil and gas exploration due to the importance of fluvial reservoirs. It is difficult to describe fluvial reservoirs in detail owing to their frequent changes and serious intersections, as well as limitations of S/N ratio and seismic data resolution. Based on the Laohekou 3D data in Shengli Oilfield, we analyze the general characteristics of fluvial reservoirs in this area, from which we find that they are characterized by strong amplitudes on seismic profiles, high continuity on time slices, and low frequency in the frequency domain. In addition, a cluster of strong string-bead- like reflections was found after color processing and detailed interpretation. To understand this observation, we conduct forward modeling to explain the mechanism. This provides a new way to identify ancient channels in similar areas. By using the multi-attribute fusion and RGB display techniques, channel incision is more obvious and the characteristics of the channel structures are manifested much better. Finally, we introduce and apply multi-wavelet detection technology to identify weaker fluvial reservoir signals.
文摘Aim To get the theory base of designing FM fuze's jamming signal, its jamming mechanism was studied. Methods A sinusoidal FM fuze was analyzed in time domain and frequency domain and the concept of channel leak was presented. Results It was proved that information channel leak exists in FM fuze because of the nonlinear property of the mixer. The jamming signal was designed based on the channel leak and the jamming mechanism was analyzed in detail. Conclusion This kind of jamming signal can jam the sinusoidal FM fuzes effectively just depending on the jamming signal's feature itself. It's different from the traditional jamming way of simulating echo. Though the sinusoidal FM fuze was just analyzed, the principle is applicable to all FM fuzes. At the same time, it may be used as the reference for FM radar and communication countermeasures.
文摘Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA) in Vicia faba. Stomatal opening was completely inhibited by external application of 1 μmol/L ABA, and such ABA inhibition was significantly reversed by the addition of CDPK inhibitor trifluoperazine (TFP). The inward whole cell K + currents were inhibited by 60% in the presence of 1 μmol/L intracellular ABA, and this inhibition was completely abolished by the addition of CDPK competitive substrate histone Ⅲ S. The results suggest that CDPKs may be involved in the signal transduction cascades of ABA regulated stomatal movements.
文摘Objective To investigate effect of pinacidil, an ATP sensitive potassium channel (KATP) opener, on the neuronal apoptosis and its signaling transduction mechanism following focal cerebral ischemia-reperfusion in rats. Methods One hundred male Wistar rats were randomly divided into four groups: A, sham-operated group; B, ischemia-reperfusion group; C, KATe opener treatment group; and D, KATe opener and blocker treatment group. The middle cerebral artery occlusion (MCAO) model was established by using the intraluminal suture occlusion method, neuronal apoptosis was determined by TUNEL staining, and expressions of caspase-8, caspase-9 and caspase-3 mRNA were detected by in situ hybridization. Results (1) The numbers of apoptotic neurons at 12 h, 24 h, 48 h, and 72 h were significantly less in group C than in groups B and D (P 〈 0.01 or P 〈 0.05); and there was no difference between groups B and D at all time points (P 〉 0.05). (2) The expressions of caspase-3 mRNA and caspase-8 mRNA at all times and the expressions of caspase-9 mRNA at 12 h, 24 h, 48 h, 72 h were significantly lower in group C than in groups B and D (P 〈 0.01 or P 〈 0.05); and there were no differences between groups B and D at all time points (P 〉 0.05). Conclusions KATP opener can significantly decrease the neuronal apoptosis and the expressions of caspase-3, caspase-8 and caspase-9 mRNAs following cerebral ischemiareperfusion. The neuronal apoptosis may be decreased by the inhibition of both mitochondrial and death-receptor signal pathways.
基金supported by National Natural Science Foundation of China(61521003,61501516,61471396,61401510)Postdoctoral Science Foundation of China(2016M592990)
文摘A novel secret key generation(SKG)method based on two-way randomness is proposed for TDD-SISO system.The legitimate transceivers mutually transmit their own random signal via reciprocal wireless channel,then the multiplication of transmitted and received signal is used as common randomness to generate secret keys.In quasi-static channel,the theoretical SKG rates(SKGRs)of the three SKG methods,namely wireless channel based,one-way randomness and two-way randomness,are derived and compared.Further,two practical SKG schemes based on twoway randomness,Scheme-1bit and Scheme-3bit,are completely designed and simulated.Generally,Scheme-1bit applies to low signal to noise ratio(SNR)scenarios and achieves 0.13~0.86bit/T_s SKGR and 10^(-2)~10^(-5) level secret key outage probability(SKOP),while Scheme-3bit fits high SNR situation and obtains 0.93~1.35bit/T_s SKGR and 10^(-3)~10^(-4) level SKOP.At last,the national institute of standards and technology(NIST)test is conducted to evaluate the secret key randomness(SKRD)and the test results show that both of the proposed schemes have passed the test.
基金The Natural Science Foundation of Jilin Province(No.20190201107JC)the National Key Research and Development Program of China(No.2019YFB1600500)。
文摘In order to improve the operational efficiency of heavy left-turn demand intersections,an optimal allocation model of an intersection with dynamic use of exit lanes for left turns(EFL)is proposed.The constraints of setting EFL are analyzed,including the number and length of reverse variable lanes,flow direction constraints,and signal constraints,etc.The constraints and control variables are combined in a unified framework for simultaneous optimization.The objective functions are defined as the average delay and left-turn capacity of an intersection.The model is solved by a non-dominated genetic algorithm(NSGA-Ⅱ).The results show that after the optimal allocation of EFL,the average vehicle delays of the intersection can be reduced by 14.9%and left-turn capacity can be increased by 19.3%.The effectiveness of the optimal allocation model of EFL is demonstrated.
基金Supported by the National Natural Science Foundation of China,No. 39880032Major Programs for Science and Technology Development of Guangzhou, No. 01-Z-005-01
文摘AIM: To develop a simplified and efficient method for the preparation of hepatitis C virus (HCV) cDNA microarray probes.METHODS: With the technique of restriction display PCR (RD-PCR), restriction enzyme Sau3A I was chosen to digest the full-length HCV cDNAs. The products were classified and re-amplified by RD-PCR. We separated the differential genes by polyacrylamide gel electrophoresis and silver staining. Single bands cut out from the polyacrylamide gel were isolated. The third-round PCR was performed using the single bands as PCR template.The RD-PCR fragments were purified and cloned into the pMD18-T vector. The recombinant plasmids were extracted from positive clones, and the target gene fragments were sequenced. The cDNA microarray was prepared by spotting RD-PCR products to the surface of amino-modified glass slides using a robot. We validated the detection of microarray by hybridization and sequence analysis.RESULTS: A total of 24 different cDNA fragments ranging from 200 to 800 bp were isolated and sequenced,which were the specific gene fragments of HCV. These fragments could be further used as probes in microarray preparation. The diagnostic capability of the microarray was evaluated after the washing and scanning steps. The results of hybridization and sequence analysis showed that the specificity, sensitivity, accuracy, reproducibility,and linearity in detecting HCV RNA were satisfactory.CONCLUSION: The RD-PCR technique is of great value in obtaining a large number of size-comparable gene probes, which provides a speedy protocol in generating probes for the preparation of microarrays. Microarray prepared as such could be further optimized and applied in the clinical diagnosis of HCV.
文摘There is evidence to suggest that follicle-stimulating hormone (FSH) can facilitate the neovascularization of ovarian cancers by increasing vascular endothelial growth factor (VEGF) expression in cancer cells, although the underlying molecular mechanism of this process is not well known. Therefore, we investigated the effect of FSH on VEGF expression in the ovarian cancer cell lines SKOV-3 and ES-2. Treatment with FSH significantly increased VEGF expression in a dose- and time-dependent manner. In addition, FSH treatment enhanced the expression of survivin and hypoxlainducible factor-1 (HIF-1α). Knockdown of survivin or HIF-1α suppressed VEGF expression, but only knockdown of survivin inhibited FSH-stimulated VEGF expression. Pretreatment with LY294002, a phosphoinositide 3-kinase (PI3K)/AKT inhibitor, neutralized the enhanced expression of survivin induced by FSH, but treatment with U0126, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor, had no such effect. We further showed that ovarian serous cystadenocarcinoma samples had much higher incidence of positive AKT and phosphorylated AKT (pAKT) protein staining than did benign ovarian cystadenoma samples (p 〈 0.01). The 5-year survival rate was only about 15% in patients with ovarian serous cystadenocarcinoma who had AKT and pAKT expression, whereas it was about 80% in those who did not have AKT or pAKT expression. Taken together, these results indicate that FSH increases the expression of VEGF by upregulating the expression of survivin, which is activated by the PI3K/AKT signaling pathway. Understanding the role of the PI3K/AKT pathway in FSH-stimulated expression of survivin and VEGF will be beneficial for evaluating the prognosis for patients with ovarian serous cystadenocarcinoma and for pursulug effective treatment against this disease.
文摘L (2, 1)-labeling number, λ(G( Z , D)) , of distance graph G( Z , D) is studied. For general finite distance set D , it is shown that 2D+2≤λ(G( Z , D))≤D 2+3D. Furthermore, λ(G( Z , D)) ≤8 when D consists of two prime positive odd integers is proved. Finally, a new concept to study the upper bounds of λ(G) for some special D is introduced. For these sets, the upper bound is improved to 7.
基金Supported by the High Technology Research and Development Program of China (No.2001AA602021).
文摘The intelligent pig based on the (MFL) is frequently used for in-line inspection of transportation pipelines. The article discusses the key technology of an MFL tool that includes the sensors structure, the constitution of tool hardware, software and the analysis method of MFL signal.
基金supported by the National Natural Science Foundation of China(NSFC) under Grant No.61201189 and 61132002National High Tech(863) Projects under Grant No.2011AA010202+1 种基金Research Fund of Tsinghua University under Grant No.2011Z05117 and 20121087985Shenzhen Strategic Emerging Industry Development Special Funds under Grant No. CXZZ20120616141708264
文摘Signal-to-noise ratio (SNR) and channel estimations are critical for 60-GHz communications to track the optimal trans- mission and reception beam pairs. However, the excessive pilot overhead for the estima- tions severely reduces system throughput in fast-rotation scenarios. In order to address this problem, we firstly demonstrate the potential sparseness property of 60-GHz channel in beam tracking; subsequently, via exploiting this property, we propose a novel compressed SNR-and-channel estimation. The estimation is conducted in a three-stage fashion, includ- ing the unstructured estimation, nonzero-tap detection, and structured estimation with non- zero-tap location. Numerical simulations show that, in the case of substantial reduction of the pilot overhead, the proposed estimator still reveals a significant improvement in terms of estimation performance over the scheme in IEEE 802.1 lad. Furthermore, it is also demon- strated that the proposed SNR and channel estimators can approach the lower bounds in sparse channels so long as SNR exceeds 8 dB.
基金National Key R&D Program of China(No.2019YFB1600501)Scientific and Technological Developing Project of Jilin Province(No.20190201107JC).
文摘To reduce the delay of left-turning buses and improve the traffic efficiency at signalized intersections,a novel variable bus approach lane(VBAL)control method based on bus pre-signals is proposed.This method combines the variable lane with the bus priority pre-signal,and realizes the left-turning bus priority without causing great impact on other vehicles.To validate the effectiveness of the method,the VBAL scheme was compared with the single left-turn lane scheme(SLTL)and the double left-turn lane scheme(DLTL).On this basis,the delay change calculation model of left-turning buses and through vehicles were established by the cumulative curve graphic method.The influence of vehicle proportion and green split on the model was studied through sensitivity analysis.The results show that VBAL can reduce the delay of left-turning bus and the increase of through vehicle delay to the greatest extent.Finally,the scheme was applied to a real-world intersection,and the results demonstrate the effectiveness and advantage of the VBAL scheme.
基金The National Natural Science Foundation of China(No.10971025,10901035)
文摘For a graph G and two positive integers j and k an m-L j k -edge-labeling of G is an assignment from the set 0 1 … m-to the edges such that adjacent edges receive labels that differ by at least j and edges at distance two receive labels that differ by at least k.Theλ′j k-number of G denoted byλ′j k G is the minimum integer m overall m-L j k -edge-labeling of G.The necklace is a specific type of Halin graph.The L 1 2 -edge-labeling of necklaces is studied and the lower and upper bounds on λ′1 2-number for necklaces are given.Also both the lower and upper bounds are attainable.
文摘The properties of the paths in an ROBDD representation of a Boolean function are presented and proved in the present paper, and the applications of ROBDD in calculating signal probability are also discussed. By this method, the troublesome calculation of the correlation among the nodes, which is caused by the re-convergent fan-out in digital system, can be avoided and power estimation can be faster than simulation-based method in [1].