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外周血GPC3与PEG10 mRNA检测对肝细胞癌转移的诊断价值 被引量:3
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作者 张洁 王之青 +2 位作者 于嘉屏 李继强 葛海良 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2010年第2期208-212,共5页
目的研究外周血磷脂酰肌醇蛋白聚糖3(GPC-3)与遗传印迹基因(PEG10)mRNA检测对肝细胞癌(HCC)转移的诊断价值。方法30例HCC患者分为HCC转移组(n=18)和HCC未转移组(n=12),另选取11例肝硬化患者作为对照。以SYBRGreenⅠ作为荧光信号,采用实... 目的研究外周血磷脂酰肌醇蛋白聚糖3(GPC-3)与遗传印迹基因(PEG10)mRNA检测对肝细胞癌(HCC)转移的诊断价值。方法30例HCC患者分为HCC转移组(n=18)和HCC未转移组(n=12),另选取11例肝硬化患者作为对照。以SYBRGreenⅠ作为荧光信号,采用实时荧光定量聚合酶链反应(FQ RT-PCR)检测三组患者外周血GPC-3 mRNA和PEG10 mRNA表达,应用ROC曲线和诊断性能专用指标评价两者预测诊断及排除诊断的价值。结果HCC转移组GPC-3 mRNA和PEG10 mRNA表达显著高于HCC未转移组和肝硬化组(P<0.05);HCC未转移组与肝硬化组GPC-3 mRNA和PEG10 mRNA的表达比较差异无统计学意义。单项检测GPC-3 mRNA和PEG10 mRNA的诊断灵敏度分别为66.7%和72.2%,特异度均为91.7%,ROC曲线下面积(AUC)为0.748和0.812;两基因检测平行试验的灵敏度为90.7%,特异度为84.0%,阴性似然比为0.11,诊断准确率为83.3%;系列试验的灵敏度为60.5%,特异度为98.7%,阳性似然比为45.5,诊断准确率为73.3%。结论检测外周血GPC-3mRNA和PEG10 mRNA对估计肝癌有无血行播散和肝外转移有一定的价值,且PEG10 mRNA优于GPC-3 mRNA。两者联合系列试验有助于HCC外周血转移的预测诊断。 展开更多
关键词 肝细胞癌 转移 磷脂酰肌醇蛋白聚糖3基因 遗传印迹基因 ROC曲线 联合检测
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Plant Genomic Imprinting and Its Effect on Seed Development 被引量:1
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作者 孙超 唐天向 +6 位作者 唐伟杰 隋丽波 张慧 夏张婷 赵海洋 韩月鑫 林良斌 《Agricultural Science & Technology》 CAS 2017年第6期984-987,1032,共5页
Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs ... Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner, and was first discovered in mammalian embryos. Recent studies have shown that it also occurs in developing plant seeds, and is now becoming a hot topic of biology of plant seed development. According to the previous studies on imprinted genes, imprinting mechanism and their roles in plant seed development, the current progress of genomic imprinting in plant seed development was summarized and possible strategies were proposed to deal with the problems, which could provide helpful information for further research. 展开更多
关键词 Plant seed Genomic imprinting EPIGENETICS
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