This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn ( Macrobrachium nipponense ) ...This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn ( Macrobrachium nipponense ) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability (h = 1.000, π = 0.028), whereas the population from Cbongqing exhibits the lowest level of variability (h = 0.700,π = 0.008). Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (N J) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.展开更多
The Hooded Crane (Grus monacha) is a waterbird wintering in the wetlands of the middle and lower reaches of the Yangtze River, China. The gradual habitat loss resulting from wetland degradation may have posed negative...The Hooded Crane (Grus monacha) is a waterbird wintering in the wetlands of the middle and lower reaches of the Yangtze River, China. The gradual habitat loss resulting from wetland degradation may have posed negative effects on the structure of our wintering populations. For its effective protection, it is important to conduct an intensive study on the genetic structure of this population. A total of 221 faecal samples, nine feather samples and four muscle samples of Hooded Cranes from four wintering populations, i.e., from Caizi Lake and Shengjin Lake in Anhui, Poyang Lake in Jiangxi and Chongming Dongtan in Shanghai, were collected for this study. Full-length 1103–1104 bp mtDNA D-loop sequences from 72 samples were amplified using PCR. Based on our amplified D-loop sequences and the sequences of two individual birds obtained from GenBank (AB017625 and AB023813), we analyzed the genetic structure of these four wintering Hooded Crane populations. Twenty six variable sites were found among 72 target sequences in the four wintering populations and 23 haplotypes were defined. Genetic diversity analyses showed that the haplotype diversity of Hooded Cranes was 0.823 ± 0.042 with a nucleotide diversity of 0.00157 ± 0.00021. The FST values of the four populations show that there is no significant genetic differentiation among the populations of Hooded Cranes wintering in the middle and lower reaches of the Yangtze River. Tajima’s D and Fu’s tests suggest that the Hooded Crane populations may have experienced population expansion in their evolutionary history.展开更多
[Objective] This study aimed to analyze the genetic structure of eight populations of Spartina alterniflora in China at the nuclear DNA level.[Method] The EH277045-derived sequences were amplified from 75 samples in 8...[Objective] This study aimed to analyze the genetic structure of eight populations of Spartina alterniflora in China at the nuclear DNA level.[Method] The EH277045-derived sequences were amplified from 75 samples in 8 populations and directly sequenced.Nucleotide diversity,haplotype diversity,the mean value of Nei's genetic distance,genetic differentiation index FST and other genetic parameters were calculated to estimate the genetic diversity and genetic differentiation of S.alterniflora populations in China.[Result] 75 samples were divided into 25 haplotypes by 28 polymorphic sites.Relatively high nucleotide diversity(π=0.011) and haplotype diversity(Hd=0.794) were detected.The mean value of Nei's genetic distance and genetic differentiation index FST among eight populations were 0.056 and 0.222,respectively,the Nei's genetic distance ranged from 0.000 to 0.189 and FST ranged from 0.000 to 0.444 between each pair of the eight populations.AMOVA result revealed that 79% and 21% of the total genetic variation was partitioned within and among S.alterniflora populations,respectively.[Conclusion] At the nuclear DNA level,there were a relatively high level of genetic diversity and a relatively low level of genetic differentiation among S.alterniflora populations in China,and the genetic diversity existed mainly within rather than among populations.展开更多
To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamysfarreri population. Thirt...To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamysfarreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-pp, ACP, AK, PK, AAT, SOD, EST) in three butter systems (TC, pH6.9; TMME, pH 7.4; and EBT, pHS.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep.m-l, Pep.pp. Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.展开更多
Mendel, the father of genetics took the first steps in defining "contrasting characters, genotypes in F1 and F2... and setting different laws". The genotypes of F2 is dependent on the type of its parents genotype an...Mendel, the father of genetics took the first steps in defining "contrasting characters, genotypes in F1 and F2... and setting different laws". The genotypes of F2 is dependent on the type of its parents genotype and it follows certain roles. Purpose of this paper is to analyze the second generation genotypes of monohybrid and a dihybrid with a mathematical structure. We use the concept of Hv-semigroup structure in the F2-genotypes with cross oper- ation and prove that this is an Hv-semigroup. We determine the kinds of number of the Hv-subsemigroups of F2-genotypes.展开更多
Different cell types in a certain organism contain the same genome while running diverse transcription programs. This is controlled by epigenetic information, including DNA modifications, histone modifications, histon...Different cell types in a certain organism contain the same genome while running diverse transcription programs. This is controlled by epigenetic information, including DNA modifications, histone modifications, histone variants, non-coding RNAs, and chromatin architectures. Interestingly, recent progresses revealed extensive crosstalks among these epigenetic players. We discuss here how epigenetic players coordinate with each other to maintain epigenetic identities in mammal cells. More exciting researches are expected to expand our understanding regarding the epigenetic regulatory network.展开更多
基金This project was supported partly by Key Research Programof Zhejiang(2005C12006-01,2006C12005)
文摘This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn ( Macrobrachium nipponense ) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability (h = 1.000, π = 0.028), whereas the population from Cbongqing exhibits the lowest level of variability (h = 0.700,π = 0.008). Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (N J) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.
基金supported by the National Natural Science Foundation of China (Grant No 31172117)the Anhui Academic and Technical Leaders Fund
文摘The Hooded Crane (Grus monacha) is a waterbird wintering in the wetlands of the middle and lower reaches of the Yangtze River, China. The gradual habitat loss resulting from wetland degradation may have posed negative effects on the structure of our wintering populations. For its effective protection, it is important to conduct an intensive study on the genetic structure of this population. A total of 221 faecal samples, nine feather samples and four muscle samples of Hooded Cranes from four wintering populations, i.e., from Caizi Lake and Shengjin Lake in Anhui, Poyang Lake in Jiangxi and Chongming Dongtan in Shanghai, were collected for this study. Full-length 1103–1104 bp mtDNA D-loop sequences from 72 samples were amplified using PCR. Based on our amplified D-loop sequences and the sequences of two individual birds obtained from GenBank (AB017625 and AB023813), we analyzed the genetic structure of these four wintering Hooded Crane populations. Twenty six variable sites were found among 72 target sequences in the four wintering populations and 23 haplotypes were defined. Genetic diversity analyses showed that the haplotype diversity of Hooded Cranes was 0.823 ± 0.042 with a nucleotide diversity of 0.00157 ± 0.00021. The FST values of the four populations show that there is no significant genetic differentiation among the populations of Hooded Cranes wintering in the middle and lower reaches of the Yangtze River. Tajima’s D and Fu’s tests suggest that the Hooded Crane populations may have experienced population expansion in their evolutionary history.
基金Supported by National Natural Science Foundation of China(30900161)~~
文摘[Objective] This study aimed to analyze the genetic structure of eight populations of Spartina alterniflora in China at the nuclear DNA level.[Method] The EH277045-derived sequences were amplified from 75 samples in 8 populations and directly sequenced.Nucleotide diversity,haplotype diversity,the mean value of Nei's genetic distance,genetic differentiation index FST and other genetic parameters were calculated to estimate the genetic diversity and genetic differentiation of S.alterniflora populations in China.[Result] 75 samples were divided into 25 haplotypes by 28 polymorphic sites.Relatively high nucleotide diversity(π=0.011) and haplotype diversity(Hd=0.794) were detected.The mean value of Nei's genetic distance and genetic differentiation index FST among eight populations were 0.056 and 0.222,respectively,the Nei's genetic distance ranged from 0.000 to 0.189 and FST ranged from 0.000 to 0.444 between each pair of the eight populations.AMOVA result revealed that 79% and 21% of the total genetic variation was partitioned within and among S.alterniflora populations,respectively.[Conclusion] At the nuclear DNA level,there were a relatively high level of genetic diversity and a relatively low level of genetic differentiation among S.alterniflora populations in China,and the genetic diversity existed mainly within rather than among populations.
基金Supported by Chinese Basic Research Project (G1999012007) and Na-tional Natural Science Foundation of China (No. 39700017).
文摘To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamysfarreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-pp, ACP, AK, PK, AAT, SOD, EST) in three butter systems (TC, pH6.9; TMME, pH 7.4; and EBT, pHS.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep.m-l, Pep.pp. Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.
文摘Mendel, the father of genetics took the first steps in defining "contrasting characters, genotypes in F1 and F2... and setting different laws". The genotypes of F2 is dependent on the type of its parents genotype and it follows certain roles. Purpose of this paper is to analyze the second generation genotypes of monohybrid and a dihybrid with a mathematical structure. We use the concept of Hv-semigroup structure in the F2-genotypes with cross oper- ation and prove that this is an Hv-semigroup. We determine the kinds of number of the Hv-subsemigroups of F2-genotypes.
基金supported by the Chinese Ministry of Science and Technology(2015CB856200 and 2011CB965300)the National Natural Science Foundation of China(91419305 and31425013)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB08010103)the Howard Hughes Medical Institute International Early Career Scientist Program
文摘Different cell types in a certain organism contain the same genome while running diverse transcription programs. This is controlled by epigenetic information, including DNA modifications, histone modifications, histone variants, non-coding RNAs, and chromatin architectures. Interestingly, recent progresses revealed extensive crosstalks among these epigenetic players. We discuss here how epigenetic players coordinate with each other to maintain epigenetic identities in mammal cells. More exciting researches are expected to expand our understanding regarding the epigenetic regulatory network.