Fishes, the biggest and most diverse community in vertebrates are good experimental models for studies of cell and developmental biology by many favorable characteristics. Nuclear transplantation in fish has been th...Fishes, the biggest and most diverse community in vertebrates are good experimental models for studies of cell and developmental biology by many favorable characteristics. Nuclear transplantation in fish has been thoroughly studied in China since 1960s. Fish nuclei of embryonic cells from different genera were transplanted into enucleated eggs generating nucleo-cytoplasmic hybrids of adults. Most importantly, nuclei of cultured goldfish kidney cells had been reprogrammed in enucleated eggs to support embryogenesis and ontogenesis of a fertile fish. This was the first case of cloned fish with somatic cells. Based on the technique of microinjection, recombinant MThGH gene has been transferred into fish eggs and the first batch of transgenic fish were Produced in 1984. The behavior of foreign gene was characterized and the onset of the foreign gene replication occurred between the blastula to gastrula stages and random integration mainly occurred at later stages of embryogenesis. This eventually led to the transgenic mosaicism. The MThGH-transferred common carp enhanced growth rate by 2-4 times in the founder juveniles and doubled the body weight in the adults. The transgenic common carp were more efficient in utilizing dietary protein than the controls. An 'all-fish' gene construct CAgcGH has been made by splicing the common carp β-actin gene (CA) promoter onto the grass carp growth hormone gene (goGH) coding sequence. The CAgcGH-transferred Yellow River Carp have also shown significantly fast-growth trait. Combination of techniques of fish cell culture, gene transformation with cultured cells and nuclear transplantation should be able to generate ho- mogeneous strain of valuable transgenic fish to fulfil human requirement in 21st century展开更多
This paper presents the possibilities of job optimization in waterway with multiple locks and canals, in order to increase the system productivity. Safe navigation in such complex waterway system is very demanding. So...This paper presents the possibilities of job optimization in waterway with multiple locks and canals, in order to increase the system productivity. Safe navigation in such complex waterway system is very demanding. Some of the problems that need to be solved are： How to control traffic in a way that vessels move in opposite directions; How to resolve possible conflicts in case that more vessels try to acquire particular lock at the same time; How to avoid possible deadlocks; How to ensure the vessel passage in the shortest possible time？ It is necessary to apply adequate control policy to avoid deadlocks and blocks the vessels＇ moving only in the case of dangerous situation. The motion of vessels can be described as the set of discrete events and states. Herein we propose deadlock avoidance algorithm for complex waterway system with multiple key resources and we use multiple re-entrant flowlines class of Petri net （MRF^PN）. The solution represents deadlock prevention supervisor in a sense that vessels are stopped only in a case of immediate dangerous situation in dense traffic. The goal of this paper is to find optimal, conflict and deadlock free job schedule in CWS. In this sense, the authors developed the algorithm which integrates MRF^PN with a genetic algorithm. The algorithm deals with multi-constrained scheduling problem with shared resources. The final goals are minimization the total travel time of vessels through the waterway system.展开更多
文摘Fishes, the biggest and most diverse community in vertebrates are good experimental models for studies of cell and developmental biology by many favorable characteristics. Nuclear transplantation in fish has been thoroughly studied in China since 1960s. Fish nuclei of embryonic cells from different genera were transplanted into enucleated eggs generating nucleo-cytoplasmic hybrids of adults. Most importantly, nuclei of cultured goldfish kidney cells had been reprogrammed in enucleated eggs to support embryogenesis and ontogenesis of a fertile fish. This was the first case of cloned fish with somatic cells. Based on the technique of microinjection, recombinant MThGH gene has been transferred into fish eggs and the first batch of transgenic fish were Produced in 1984. The behavior of foreign gene was characterized and the onset of the foreign gene replication occurred between the blastula to gastrula stages and random integration mainly occurred at later stages of embryogenesis. This eventually led to the transgenic mosaicism. The MThGH-transferred common carp enhanced growth rate by 2-4 times in the founder juveniles and doubled the body weight in the adults. The transgenic common carp were more efficient in utilizing dietary protein than the controls. An 'all-fish' gene construct CAgcGH has been made by splicing the common carp β-actin gene (CA) promoter onto the grass carp growth hormone gene (goGH) coding sequence. The CAgcGH-transferred Yellow River Carp have also shown significantly fast-growth trait. Combination of techniques of fish cell culture, gene transformation with cultured cells and nuclear transplantation should be able to generate ho- mogeneous strain of valuable transgenic fish to fulfil human requirement in 21st century
文摘This paper presents the possibilities of job optimization in waterway with multiple locks and canals, in order to increase the system productivity. Safe navigation in such complex waterway system is very demanding. Some of the problems that need to be solved are： How to control traffic in a way that vessels move in opposite directions; How to resolve possible conflicts in case that more vessels try to acquire particular lock at the same time; How to avoid possible deadlocks; How to ensure the vessel passage in the shortest possible time？ It is necessary to apply adequate control policy to avoid deadlocks and blocks the vessels＇ moving only in the case of dangerous situation. The motion of vessels can be described as the set of discrete events and states. Herein we propose deadlock avoidance algorithm for complex waterway system with multiple key resources and we use multiple re-entrant flowlines class of Petri net （MRF^PN）. The solution represents deadlock prevention supervisor in a sense that vessels are stopped only in a case of immediate dangerous situation in dense traffic. The goal of this paper is to find optimal, conflict and deadlock free job schedule in CWS. In this sense, the authors developed the algorithm which integrates MRF^PN with a genetic algorithm. The algorithm deals with multi-constrained scheduling problem with shared resources. The final goals are minimization the total travel time of vessels through the waterway system.
