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细菌基因组稳定性和变异性之间动态平衡的自发调控:遗传开关的作用及其分子机制 被引量:3
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作者 赵尔樱 金刚 +5 位作者 唐乐 周玉洁 魏晓维 王冠 刘桂荣 刘树林 《中国科学:生命科学》 CSCD 北大核心 2013年第5期361-367,共7页
细菌在进化的过程中要保持高度的遗传稳定性来实现种系的繁衍,但同时也需要一定的变异性来适应环境的变化.这一对看似尖锐的矛盾在细菌基因组内却实现了完美的调和.已有大量研究确证,细菌的遗传稳定性主要是由DNA错配修复系统维持的.那... 细菌在进化的过程中要保持高度的遗传稳定性来实现种系的繁衍,但同时也需要一定的变异性来适应环境的变化.这一对看似尖锐的矛盾在细菌基因组内却实现了完美的调和.已有大量研究确证,细菌的遗传稳定性主要是由DNA错配修复系统维持的.那么,遗传变异性又是怎么实现的?这个问题长期没有明确的答案.最近的细菌比较基因组学研究结果表明,错配修复系统中的mutL基因可由一个6碱基重复序列拷贝数的自发变换来调控细菌的遗传稳定性和变异性之间的动态平衡,起到遗传开关的作用.本文对遗传开关的发现和理论提出过程进行了详述,并对其在细菌进化中的作用进行了展望. 展开更多
关键词 细菌进化 错配修复 遗传开关 比较基因组学 沙门菌基因组
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遗传拨动开关系统的全局稳定性 被引量:1
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作者 李洁 何志蓉 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2017年第4期675-678,共4页
本文研究一个具有协同数为1的遗传拨动开关系统的全局定性性质.本文首先证明该系统仅有一个平衡点且为稳定结点,再利用Poincaré-Bendixson定理证明系统没有周期轨,最后证明系统恰有两个无穷远平衡点且均为鞍结点,从而获得系统的全... 本文研究一个具有协同数为1的遗传拨动开关系统的全局定性性质.本文首先证明该系统仅有一个平衡点且为稳定结点,再利用Poincaré-Bendixson定理证明系统没有周期轨,最后证明系统恰有两个无穷远平衡点且均为鞍结点,从而获得系统的全局定性结构,并由此知系统是全局单稳的. 展开更多
关键词 遗传拨动开关 Poincare-Bendixson定理 周期轨 Poincare变换 无穷远平衡点
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光控诱导重组系统的开发与应用
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作者 谢甜 王梅 +3 位作者 高瑞钰 苗艳尼 张燚铭 蒋婧 《遗传》 CAS CSCD 北大核心 2022年第8期655-671,共17页
位点特异性重组系统由重组酶和特异性识别位点两部分组成,是一种强大的基因操作工具,被广泛运用于生命科学研究。已开发的诱导型重组系统以时空方式精准调控细胞和动物的基因表达,被用于基因功能研究、细胞谱系示踪和疾病治疗等领域。... 位点特异性重组系统由重组酶和特异性识别位点两部分组成,是一种强大的基因操作工具,被广泛运用于生命科学研究。已开发的诱导型重组系统以时空方式精准调控细胞和动物的基因表达,被用于基因功能研究、细胞谱系示踪和疾病治疗等领域。根据诱导重组酶时空表达方式的不同,诱导型重组系统可分为化学诱导和光控诱导两种方式。光控诱导重组系统是利用光作为诱导剂,根据光控方式和对象的不同,可进一步分为光笼和光遗传学两类。光笼诱导重组系统是利用光敏基团来控制化学诱导剂或重组酶,光诱导前它们的活性被光敏基团抑制;在特定光照射后,它们的活性被恢复,进而实现光控诱导基因重组。光遗传学诱导重组系统是通过光遗传学开关介导分割型重组酶的重新激活来诱导基因重组。其中光遗传学开关由一系列基因编码的光敏蛋白组成,包括隐花色素、VIVID蛋白、光敏色素等。这些类型丰富的光控诱导重组系统为从高时空分辨率的维度解析基因的表达和功能提供了更多的工具,以满足日益复杂的生命科学研究需求。本文主要对不同类型光控诱导重组系统的开发原理及应用进行综述,比较其优缺点,最后对未来开发更多光控重组系统进行展望,旨在为系统优化升级提供理论基础和指导。 展开更多
关键词 光控诱导重组系统 光笼 遗传开关 位点特异性重组酶 时空调控
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THE EFFECTIVENESS OF GENETIC ALGORITHM IN CAPTURING CONDITIONAL NONLINEAR OPTIMAL PERTURBATION WITH PARAMETERIZATION “ON-OFF” SWITCHES INCLUDED BY A MODEL 被引量:2
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作者 方昌銮 郑琴 《Journal of Tropical Meteorology》 SCIE 2009年第1期13-19,共7页
In the typhoon adaptive observation based on conditional nonlinear optimal perturbation (CNOP), the ‘on-off’ switch caused by moist physical parameterization in prediction models prevents the conventional adjoint me... In the typhoon adaptive observation based on conditional nonlinear optimal perturbation (CNOP), the ‘on-off’ switch caused by moist physical parameterization in prediction models prevents the conventional adjoint method from providing correct gradient during the optimization process. To address this problem, the capture of CNOP, when the "on-off" switches are included in models, is treated as non-smooth optimization in this study, and the genetic algorithm (GA) is introduced. After detailed algorithm procedures are formulated using an idealized model with parameterization "on-off" switches in the forcing term, the impacts of "on-off" switches on the capture of CNOP are analyzed, and three numerical experiments are conducted to check the effectiveness of GA in capturing CNOP and to analyze the impacts of different initial populations on the optimization result. The result shows that GA is competent for the capture of CNOP in the context of the idealized model with parameterization ‘on-off’ switches in this study. Finally, the advantages and disadvantages of GA in capturing CNOP are analyzed in detail. 展开更多
关键词 dynamic meteorology typhoon adaptive observation genetic algorithm conditional nonlinear optimal perturbation switches moist physical parameterization
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调控进化与形态多样性 被引量:1
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作者 国春策 张睿 +1 位作者 山红艳 孔宏智 《生物多样性》 CAS CSCD 北大核心 2014年第1期72-79,共8页
揭示导致生物体形态和结构多样性产生的原因和机制,是进化生物学研究的重要内容。进化发育生物学的研究表明,许多复杂的形态结构及其多样性,都是通过对古老调控网络的修饰或改造来完成的。也就是说,生物体形态和结构的多样化并不是像以... 揭示导致生物体形态和结构多样性产生的原因和机制,是进化生物学研究的重要内容。进化发育生物学的研究表明,许多复杂的形态结构及其多样性,都是通过对古老调控网络的修饰或改造来完成的。也就是说,生物体形态和结构的多样化并不是像以前认为的是由基因编码区的变化造成的,而更多的是取决于基因的调控进化。作为控制基因表达的关键组分,基因调控区的顺式调控元件通过与特定反式作用因子结合,精细调控基因表达的时、空和量。因此,调控元件的获得、丢失、修饰或者改变都能引起基因表达模式的变化,是形态和结构多样性产生的主要原因。本文结合近年来国际上在基因的调控进化方面所取得的进展,总结了真核生物中基因调控的方式和特点,阐述了调控进化的基本式样,揭示了调控进化在生物进化(特别是形态和结构多样化)中的作用。 展开更多
关键词 调控元件 多效基因 遗传开关 平行演化 进化发育生物学
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An effective genetic algorithm to VDA with discontinuous “on-off” switches 被引量:4
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作者 ZHENG Qin SHA JianXin FANG ChangLuan 《Science China Earth Sciences》 SCIE EI CAS 2012年第8期1345-1357,共13页
With more and more improvements of atmosphere or ocean models,a growing number of physical processes in the form of parameterization are incorporated into the models,which,on the one hand,makes the models capable of d... With more and more improvements of atmosphere or ocean models,a growing number of physical processes in the form of parameterization are incorporated into the models,which,on the one hand,makes the models capable of describing the at-mospheric or oceanic movement more precisely,and on the other hand,introduces non-smoothness in the form of "on-off" switches into the models."On-off" switches enhance the nonlinearity of the models and finally result in the loss of the effec-tiveness of variational data assimilation(VDA) based on the conventional adjoint method(ADJ).This study,in virtue of the optimization ability of a genetic algorithm(GA) for non-smooth problems,presents a new GA(referred to as GA NEW) to solve the problems of the VDA with discontinuous "on-off" processes.In the GA-NEW,adaptive selection and mutation oper-ators,blend crossover operator,and elitist strategy are combined in application.In order to verify the effectiveness and feasi-bility of the GA NEW in VDA,an idealized model of partial differential equation with discontinuous "on-off" switches in the forcing term is adopted as the governing equation.By comparison with the ADJ,it is shown that the GA NEW in VDA is more effective and can yield better assimilation retrievals.In addition,VDA experiments demonstrate that the performance of a GA is greatly related to the configuration of genetic operators(selection,crossover and mutation operators) and much better results may be attained with more proper genetic operations.Furthermore,the robustness of the GA NEW to observational noise,model errors and observation density is investigated,and the results show that the GA NEW has stronger robustness than the ADJ with respect to all the three observation noises,model errors,and sparse observation. 展开更多
关键词 variational data assimilation "on-off" switches genetic algorithm
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Spontaneous modulation of a dynamic balance between bacterial genomic stability and mutability: roles and molecular mechanisms of the genetic switch 被引量:1
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作者 TANG Le LIU RuoWei +3 位作者 JIN Gang ZHAO ErYing LIU GuiRong LIU ShuLin 《Science China(Life Sciences)》 SCIE CAS 2014年第3期275-279,共5页
Bacteria need a high degree of genetic stability to maintain their species identities over long evolutionary times while retaining some mutability to adapt to the changing environment.It is a long unanswered question ... Bacteria need a high degree of genetic stability to maintain their species identities over long evolutionary times while retaining some mutability to adapt to the changing environment.It is a long unanswered question that how bacteria reconcile these seemingly contradictory biological properties.We hypothesized that certain mechanisms must maintain a dynamic balance between genetic stability and mutability for the survival and evolution of bacterial species.To identify such mechanisms,we analyzed bacterial genomes,focusing on the Salmonella mismatch repair(MMR)system.We found that the MMR gene mutL functions as a genetic switch through a slipped-strand mispairing mechanism,modulating and maintaining a dynamic balance between genetic stability and mutability during bacterial evolution.This mechanism allows bacteria to maintain their phylogenetic status,while also adapting to changing environments by acquiring novel traits.In this review,we outline the history of research into this genetic switch,from its discovery to the latest findings,and discuss its potential roles in the genomic evolution of bacteria. 展开更多
关键词 genetic switch genomic evolution mismatch repair comparative genomics Salmonella genome
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