The authors report a Japanese patient with hereditary sensory and autonomic neuropathy type 2 (HSAN2) who has a new mutation of the HSN2 gene. The pathologic findings of the patient matched those of Canadian patients....The authors report a Japanese patient with hereditary sensory and autonomic neuropathy type 2 (HSAN2) who has a new mutation of the HSN2 gene. The pathologic findings of the patient matched those of Canadian patients. They identified a homozygous 1134-1135 ins T mutation, resulting in a frameshift, and the subsequent premature stop codon at residue 378. These observations support the hypothesis that HSN2 is a causative gene for HSAN2.展开更多
目的探讨基于二代测序的植入前单体型分析在遗传性感觉和自主神经病4型(hereditary sensory and autonomic neuropathyⅣ,HSANⅣ)出生缺陷阻断中的应用价值。方法纳入1个HSANⅣ家系,采用高通量测序和Sanger测序相结合的方法对NTRK1基因...目的探讨基于二代测序的植入前单体型分析在遗传性感觉和自主神经病4型(hereditary sensory and autonomic neuropathyⅣ,HSANⅣ)出生缺陷阻断中的应用价值。方法纳入1个HSANⅣ家系,采用高通量测序和Sanger测序相结合的方法对NTRK1基因进行突变分析,通过构建TrkA表达载体对突变进行致病性预测。对活检的囊胚滋养层细胞进行全基因组扩增,选择致病基因上下游数十至数百个单核苷酸多态性位点作为连锁遗传标记,通过基于二代测序的植入前单体型分析完成植入前遗传学检测。结果先证者NTRK1基因存在复合杂合突变,包括新发现的移码突变(c.963delG;p.Val321-Valfs*149)和已报道的错义突变(c.850+1G>A),新突变可导致编码蛋白截短与功能失调,其母亲和父亲分别为上述突变携带者。经植入前遗传学检测,活检的2个囊胚中1个携带母方致病基因,1个受累。选择携带母方致病基因的胚胎进行移植,未获妊娠。结论本研究是我国NTRK1基因突变行植入前遗传学检测的首例报道,新发现的c.963delG突变丰富了NTRK1基因的突变谱,同时为阻断单基因遗传病出生缺陷的发生提供有效的预防手段。展开更多
Mitochondrial DNA (mtDNA) A7445G point mutation has been shown to be responsible for familial nonepidermolytic palmoplantar keratoderma (NEPPK) associated with deafness without any additional features. To date, only a...Mitochondrial DNA (mtDNA) A7445G point mutation has been shown to be responsible for familial nonepidermolytic palmoplantar keratoderma (NEPPK) associated with deafness without any additional features. To date, only a few cases have been described. We report a Portuguese pedigree presenting an inherited combination of NEPPK and sensorineural deafness compatible with maternal transmission. Clinical expression and age of onset of NEPPK and deafness were variable. Normal expression patterns of epidermal keratins and filaggrin, intercellular junction proteins including connexin 26, loricrin and cornified envelope proteins, were observed. Molecular analysis revealed that all the affected members, previously screened for Cx26 mutations with negative results, presented the mtDNA A7445G point mutation in the homoplasmic form. To our knowledge, this is the fifth family in whom inherited NEPPK and hearing loss are related to this mitochondrial mutation.展开更多
文摘遗传性感觉自主神经病(hereditary sensory autonomic neuropathy,HSAN),是一组以感觉障碍为主的遗传性周围神经病,具有临床及遗传异质性。临床表现为四肢对称性感觉减退、肌无力和肌肉萎缩,伴有自主神经功能障碍[1-2]。遗传性感觉自主神经病1型(HSAN1)是一种罕见的常染色体显性遗传病,为HSAN最常见的类型,主要表现为肢体远端感觉功能障碍[3-4]。本文探讨一例SPTLC1(serine palmitoyltransferase long chain base subunit 1)基因突变所致HSAN1的临床、病理、电生理和遗传学特点,给予L-丝氨酸口服及足部正畸治疗随访观察,对其基因突变进行分析。
文摘The authors report a Japanese patient with hereditary sensory and autonomic neuropathy type 2 (HSAN2) who has a new mutation of the HSN2 gene. The pathologic findings of the patient matched those of Canadian patients. They identified a homozygous 1134-1135 ins T mutation, resulting in a frameshift, and the subsequent premature stop codon at residue 378. These observations support the hypothesis that HSN2 is a causative gene for HSAN2.
文摘目的探讨基于二代测序的植入前单体型分析在遗传性感觉和自主神经病4型(hereditary sensory and autonomic neuropathyⅣ,HSANⅣ)出生缺陷阻断中的应用价值。方法纳入1个HSANⅣ家系,采用高通量测序和Sanger测序相结合的方法对NTRK1基因进行突变分析,通过构建TrkA表达载体对突变进行致病性预测。对活检的囊胚滋养层细胞进行全基因组扩增,选择致病基因上下游数十至数百个单核苷酸多态性位点作为连锁遗传标记,通过基于二代测序的植入前单体型分析完成植入前遗传学检测。结果先证者NTRK1基因存在复合杂合突变,包括新发现的移码突变(c.963delG;p.Val321-Valfs*149)和已报道的错义突变(c.850+1G>A),新突变可导致编码蛋白截短与功能失调,其母亲和父亲分别为上述突变携带者。经植入前遗传学检测,活检的2个囊胚中1个携带母方致病基因,1个受累。选择携带母方致病基因的胚胎进行移植,未获妊娠。结论本研究是我国NTRK1基因突变行植入前遗传学检测的首例报道,新发现的c.963delG突变丰富了NTRK1基因的突变谱,同时为阻断单基因遗传病出生缺陷的发生提供有效的预防手段。
文摘Mitochondrial DNA (mtDNA) A7445G point mutation has been shown to be responsible for familial nonepidermolytic palmoplantar keratoderma (NEPPK) associated with deafness without any additional features. To date, only a few cases have been described. We report a Portuguese pedigree presenting an inherited combination of NEPPK and sensorineural deafness compatible with maternal transmission. Clinical expression and age of onset of NEPPK and deafness were variable. Normal expression patterns of epidermal keratins and filaggrin, intercellular junction proteins including connexin 26, loricrin and cornified envelope proteins, were observed. Molecular analysis revealed that all the affected members, previously screened for Cx26 mutations with negative results, presented the mtDNA A7445G point mutation in the homoplasmic form. To our knowledge, this is the fifth family in whom inherited NEPPK and hearing loss are related to this mitochondrial mutation.
