The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals fr...The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability.展开更多
Objective To investigate the BRCA1 gene in hereditary breast and ovarian cancer, early onset breast cancer and sporadic ovarian cancer Methods The exons of 2, 11 and 20 of BRCA1 ...Objective To investigate the BRCA1 gene in hereditary breast and ovarian cancer, early onset breast cancer and sporadic ovarian cancer Methods The exons of 2, 11 and 20 of BRCA1 gene were analyzed Polymerase chain reaction single strand conformation analysis(PCR SSCP) and PCR SSCP combined by restriction enzymes were used to screen for mutations Mutations were further indentifed by sequencing. The loss of heterozygosity (LOH)were also investigated at the BRCA1 genetic loci D17S855 in 10 hereditary ovarian cancer Results A insertion mutation was detected in H7.“C” was inserted at nucleotide 797. It would result in truncation of the BRCA1 protein at codon 277. A missen mutation was detected in an early onset breast cancer(diagnosed at age 24). At nucleotide position 3732, the substitution of a “G” to a “C” in codon 1205 changes a Gly to a Arg. A missen mutation were also detected in three sporadic ovarian cancers. At nucleotide position 2051, the substitution of a “T” to a “G” in codon 644 changes a Cys to a Trp. H3 and H7 patients show LOH. Conclusions. BRCA1 gene has an important effect in Chinese hereditary breast and ovarian cancer, its effect on early onset breast cancer and sporadic ovarian cancer are still to be studied. BRCA1 gene is a tumor suppressor gene.展开更多
The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food fish.It is native to Afganistan,Pakistan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambod...The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food fish.It is native to Afganistan,Pakistan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambodia and Thailand(Froese and Pauly,2003).In India,the species in-habits the Indus,Ganges and Mahanadi river sys-terns.It is identified as a potential cultivable species and has considerable significance for the fishery of these regions.L.dyocheilus has been categorized as a vulnerable fish due to declining commercial catches in India(Mahanta et a1.,1994).At present the fishery is supported through capture from natural sources.To achieve domestication,various aspects of captive breeding and propagation are being studied.展开更多
基金Supported by the Open Funds of Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology (No. 2007HS015)the Shandong Post-Doctoral Science Foundation (No. 200802039)the China Postdoctoral Science Foundation (No. 20060390999)
文摘The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability.
文摘Objective To investigate the BRCA1 gene in hereditary breast and ovarian cancer, early onset breast cancer and sporadic ovarian cancer Methods The exons of 2, 11 and 20 of BRCA1 gene were analyzed Polymerase chain reaction single strand conformation analysis(PCR SSCP) and PCR SSCP combined by restriction enzymes were used to screen for mutations Mutations were further indentifed by sequencing. The loss of heterozygosity (LOH)were also investigated at the BRCA1 genetic loci D17S855 in 10 hereditary ovarian cancer Results A insertion mutation was detected in H7.“C” was inserted at nucleotide 797. It would result in truncation of the BRCA1 protein at codon 277. A missen mutation was detected in an early onset breast cancer(diagnosed at age 24). At nucleotide position 3732, the substitution of a “G” to a “C” in codon 1205 changes a Gly to a Arg. A missen mutation were also detected in three sporadic ovarian cancers. At nucleotide position 2051, the substitution of a “T” to a “G” in codon 644 changes a Cys to a Trp. H3 and H7 patients show LOH. Conclusions. BRCA1 gene has an important effect in Chinese hereditary breast and ovarian cancer, its effect on early onset breast cancer and sporadic ovarian cancer are still to be studied. BRCA1 gene is a tumor suppressor gene.
基金This research was funded by agrant from ICAR(No NBFGR/DNA25A)
文摘The Labeo dyocheilus(family Cyprinidae)in-habits fresh water streams at the fo thill regions and is a commercially important food fish.It is native to Afganistan,Pakistan,India,Nepal,Bhutan,Bangladesh,M yanmnar,Cambodia and Thailand(Froese and Pauly,2003).In India,the species in-habits the Indus,Ganges and Mahanadi river sys-terns.It is identified as a potential cultivable species and has considerable significance for the fishery of these regions.L.dyocheilus has been categorized as a vulnerable fish due to declining commercial catches in India(Mahanta et a1.,1994).At present the fishery is supported through capture from natural sources.To achieve domestication,various aspects of captive breeding and propagation are being studied.
