家畜遗传资源多样性的研究

家畜遗传资源,是在家畜中存在的遗传性变异,能应用于家畜育种事业。现代家畜育种在畜牧生产中占据主导地位.而某些提高了家畜经济效益、个体产量的育成品种,则会使得大量地方品种灭绝或数量大幅度减少,为防止家畜遗传资源的进一步破坏和枯竭,以原位保存为主,加入冷冻保存,繁殖技术,基因定位,DNA文库等新技术来保护家畜遗传资源必不可少。

中国—东盟生物多样性廊道内遗传资源的知识产权保护

中国—东盟自由贸易区内生物遗传资源丰富,但随着区域内经济的发展,生物多样性遭到了威胁,为此双方酝酿建设中国—东盟生物多样性廊道。当前廊道内遗传资源面临盗掘滥用、外来生物入侵、孤立保护、利益归属不清晰等问题。必须把廊道内遗传资源保护同知识产权制度结合起来,加强跨国遗传资源的知识产权保护合作,完善廊道内各国遗传资源的立法和管理体制,并加快廊道内遗传资源的普查、登记、注册等基础工作。

白木香化学成分和遗传多样性研究进展

白木香[Aquilaria sinensis(Lour.)Gilg]是珍贵的药用树种,是国产沉香的唯一植物资源,具有极高的经济价值。但由于野生资源和生态环境长期遭受人为破坏等因素,白木香野生资源量不断减少,现已被列为国家二级重点保护野生植物。本文综述了近几年白木香化学成分和种质资源遗传多样性的国内外研究进展,期望能为白木香的资源保护及综合开发利用研究提供参考。

ISSR Analysis of Genetic Relationship of Germplasm Resource in Prunus mume Sibe. et Zucc

Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.

Study on Present Status of Plant Germplasm Resources Conservation and Countermeasures

Plant germplasm resources is the material foundation and key point for agricultural production, crop breeding and bioengineering. China is one of the centers for the origin of the cultivated plants worldwide, as well as one of the countries with most abundant plant resources and the highest biodiversity. Strengthening research in the conservation and utilization of plant germplasm resources is of significant value and importance. In this study, the present status of conservation, utilization, and existing problems of plant germplasm resources in China and around the world were reviewed; further, through analyzing measures taken by global countries for plant germplasm resources protection, countermeasures for plant germplasm resources conservation in China were also presented from three aspects, namely, collection and conservation, information network construction and national legislation and policies.

Study on Genetic Diversity of Agronomic Traits and Genetic Relationships among Core Collections of Bitter Gourd

[Objective] This study aimed to investigate the genetic diversity of agronomic traits and genetic relationships among core collections of bitter gourd.[Method] Total 141 germplasms of bitter gourd were selected,and the genetic diversity of 13 agronomic traits was analyzed.In addition,total 46 core collections of bitter gourd were employed,and their genetic relationships were analyzed based on the phenotypic values and genotypic values of 5 agronomic traits,respectively.[Result] The genetic diversity analysis of agronomic traits showed that the genetic diversity indexes of the 4 qualitative traits of bitter gourd germplasms ranged from 0.46 to 1.34;the distribution of the 9 quantitative traits data was more dispersed with average coefficient of variation of 20.02%.The genetic relationship analysis showed that based on the phenotypic values and genotypic values of the 5 quantitative traits,the genetic distances among the 46 core collections of bitter gourd were different.Based on the genotypic values,the genetic distances among the 46 bitter gourd core collections ranged from 0.84 to 10.71.The 46 germplasms were divided into 17 groups with the rescaled distance of 8.5,which further classified the relationships among different germplasms.[Conclusion] This study will lay a solid foundation for the effective utilization of core collections and new variety breeding in bitter gourd.

Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L. Germplasm Resources Using ISSR Molecular Marker

[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.

Collection and Preservation of Cashew Germplasms Resources Research Progress in China

The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.

EST-SSR Marker-based Genetic Diversity Analysis of Rhododendron simsii Germplasm in Guifeng Mountain

Rhododendron simsii（Ericaeae：Rhododendron） has high ornamental value and ecological value.In this study,7 pairs of novel EST-SSR markers were developed from the genomic sequence of R.simsii,and they were used to investigate the genetic diversity of 32 natural R.simsii samples from Guifeng Mountain,Hubei Province.Results showed that a total of 31 polymorphic bands were amplified with allele number per locus of 4.43.Mean values of heterozygosity（Ho） and expected heterozygosity（He） were 0.679 58 and 0.723 14,respectively.This research will not only enrich the existing SSR database,but also lay a foundation for subsequent studies about molecular marker-assisted breeding,genetic diversity analysis,genetic structure analysis and phylogenetic analysis.

Genetic Diversity Analysis and Constructing Core Collection Based on Phenotypes in Cotton

In that DNA diversity detected nowadays could not mean phenotypic diversity,it is the precondition of breeding project and basic research of crop improvement that genetic diversity analysis and sampling of core collection by phenotypes.Phenotyping and statistic analysis on 9 traits of 92 accessions of cotton germplasm resource from three species（Gossypium hirsutum L.,Gossypium barbadence L.and Gossypium arboreum L.）were conducted.And the statistics（variation coefficient,proportion of special accessions and Shannon-Weaver information index）indicated that initial collection had abundant phenotypic diversity;software NTSYS-pc and the unweighted pair group method of arithmetic（UPGMA）were used for the cluster analysis on genetic similarity coefficient and genetic distance matrix,and the result showed that the genetic relationship among accessions was highly consistent with the pedigree;22 accessions of core collection were selected by software QGAStation,four statistics,such as variance difference percentage（VD%）,mean difference percentage（MD%）,coincidence rate（CR%）and variable rate（VR%）,showed that the genetic diversity of core collection was approximately equal to the initial collection.The results of genetic diversity analysis based on phenotypic data and sampling of core collection would provide reference for breeding projects and basic research.

Comparison of Genetic Diversity Between Local Cultivated Aromatic and Non-aromatic Rice in Yunnan Province

[ Objective] The genetic diversity of the local cultivated aromatic rice and non-aromatic rice in Yunnan Province were compared to provide further genetic resources for breeding practice. [Method] Genetic diversity of 10 aromatic rice and 45 non-aromatic rice were analyzed by 64 SSR primers covered on 12 rice chromosomes. [ Result] Per locus 5.44 and 7.98 alleles in average were detected, ranging from 2 to 12 and from 2 to 17 in aromatic and non-aromatic rice, respectively. Average genetic multiplicity index（Hs） was 0.46 and 0.67 respectively. The average polymorphism information content （PIC） was 0.43 and 0.58 in aromatic and non-aromatic rice respectively. [ Conclusion] The results indicated that genetic diversity was higher in non-aromatic rice than in aromatic rice.

Genetic Diversity of JUNCAO Germplasms Detected by i PBS Markers

[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR screening on a subset of four germplasms, of which 11 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. [Result] A total of 208 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 11 selected iPBS primers. By using the NTSYSpc 2.1 software combined with UPGMA clustering analysis method, the simple matching （SM） coefficient of similarity was calculated among all accessions and ranged from 0.58 to 0.99. The 47 JUNCAO germplasms were clustered into 10 categories at a genetic similarity coefficient of 0.67. All the 47 accessions were distinguished from each other. [Conclusion] Our results showed that iPBS markers could be effectively used for genetic diversity analysis of JUNCAO germplasms. This study provides a preliminary theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.

Research Progress on the Genetic Diversity of Wild Soybean in China

Wild soybean is recognized as the relative wild species of cultivate soy- bean. China is rich in wild soybean resources. In the past 30 years, domestic scholars have carried out a large quantity of studies on the genetic diversity of wild soybean in China, which have laid the theoretical foundation for the protection and utilization of wild soybean resources. In this paper, the research progress on the genetic diversity of wild soybean in China at the phenotypic, biochemical and molecular levels of wild soybean was reviewed, and the future development of the research on the genetic diversity of wild soybean was introduced.

Molecular Discrimination of Wild Philippine Paddy Straw Mushroom （ Volvariella volvacea）

Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA （RAPD）. A single 10-based primer was used to generate randomly amplified polymorphic DNA （RAPD） in V. volvacea and differences were noted in band size （bp） ranging from 1,800 bp to 550 bp. Principal component analysis （PCA） of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.

Analysis of 50 Cucumber Accessions by RAPD Markers

Genetic diversity of 50 cucumber (Cucumis sativus L.) accessions from American, Holland, Japan and China was detected and evaluated using RAPD markers. 25 selected primers produced 215 scorable polymorphic RAPD bands and the ratio of polymorphism is 86.98%. Four main groups of cucumber accessions could be distinguished from UPGMA analysis: Occidental cucumber, South-China cucumber, North-China cucumber and Xishuangbanna cucumber. Our result confirmed that cucumber is a narrow-based germplasm, nevertheless RAPD analysis was stili useful in cucumber genotypic differentiation. More significantly, Xishuangbanna cucumber (Cucumis sativus L. Var Xishuangbannanesis Qi et Yuan), a mutation of cucumber, was clustered together to a special group in the study, which implied its special status in cucumber germplasm.

Analysis of Genetic Diversity of JUNCAO Germplasms by SRAP Markers

SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce poly-morphic DNA fragments. As a result, a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs. By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis, the genetic similarity （SM） coefficient be- tween the 48 accessions was calculated and ranged from 0.58 to 0.98. All the 48 accessions were distinguished from each other. The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645. The results showed that the SRAP markers could be effectively used for analysis of genetic di- versify of JUNCAO germplasms. Additionally, the results also showed that there ex- ist abundant JUNCAO germptasm in the genus Arundo in China. This study provides a new technique for studying the genetic diversify of JUNCAO germplasms. The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future. Moreover, the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.

Fhe Future of TRIPS and the Trade in Genetic Resources

Since its entry into the WTO ten years ago, China has been increasingly involved in building new rules to make multilateral trading systems more development- oriented. In the past decade, both developing and developed WTO member nations have debated whether the TRIPS agreement should expand its definition to cover new areas such as genetic resources and traditional knowledge. This article argues that although the genetic resources-based market is huge, developing countries have benefited little from it. Specifically, the current market mechanism and system provide little incentive to developing countries to protect biodiversity. Expanding TRIPS to cover new areas such as genetic resources and traditional knowledge can make multilateral trade fairer and more balanced and will enable developing members to better protect biodiversity.

Study of Camelina Biodiversity in Southwestern of Algeria

In the framework of biodiversity＇s studies of animal＇s genetic resources in general and especially camels, the authors focused, in the case of biodiversity, on the phenotypic and genetic variability of each camel populations studied in southwestern of Algeria. The objective of the present study is to describe the conditions of dromedaries breeding, to analyze their phenotypic diversity, redefine the range flocks, highlight the perspectives of exploitation and use their products in livestock systems sustainablely. Knowing that the camel occupies a vital place in the socioeconomic development of breeders, owing to the multiple functions and services it provides. However, the pressures of diverse origins including population growth and recurrent droughts take serious threats to the development of the species. This survey was conducted during 2 years over 2500 animals owned by 60 farmers were studied. These animals were analyzed in terms of phenotypic and ethno-geographical evolution of breeding. A large number of blood samples （200） were collected, which allowed us to be the first DNA bank of camels in Algeria.

现代花卉产业的一场革命——基因工程在花卉育种中的应用

多年来,育种学家及爱好者利用传统的杂交育种及定向选择育种,培育出了大量观赏植物的新品种,但这种育种方法有其局限性:在自然条件下,植物变异频率较人类所需要的要小得多;人工诱变虽然提高了变异频率,但结果仍不够理想,且盲目性比较大;杂交育种虽然能弥补两者的不足,却也存在着远缘杂交不亲合,难以打破物种生殖隔离,以及在有性生殖过程中,重组时染色体的交换量很小,难以打破某些基因连锁等缺点.

The Categories and Benefit-sharing of Traditional Knowledge Associated with Biodiversity

A milestone in the Year of Biodiversity is that the Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits Arising from their Utilization to the Convention on Biological Diversity （CBD） was adopted in the 10th Conference of Parties （COP 10） held in Nagoya, Japan, Oct 18- 29, 2010. Traditional knowledge （TK） is a key issue in the Protocol’s text and its negotiating period. This paper aimed to protect TK by defining its concepts and categories and promoting benefit-sharing with TK’s holders. Based on the analysis for the TK concepts of relevant international organizations and conventions, in particularly on the author’s current years’ research work on TK in the ethnic areas of China, this paper proposes the categories and the classification system for the TK associated with biological resources, i.e. （i） the knowledge for traditional use of agricultural bio-species and genetic resources; （ii） the knowledge for traditional use of medicinal bio-species;（iii） traditional technical innovations for bio- resource use and traditional practices for farming and living styles; （iv） traditional cultures such as customary laws and community protocols that are related to conservation and sustainable use of bio-resources; and （v） traditional geographically biological indicators. Furthermore, this paper introduced the provisions for access and benefit-sharing of TK and proposed to share fairly and equitably the benefits produced from use of TK with indigenous people, local communities and other kinds of holders such as a country for the ancient documented TK. In addition, aimed to the existing problems for traditional knowledge protection, the national strategy for TK protection was introduced and measures for TK inheritance, development, utilization and protection measures were put forward, which are significant for TK’s popularization and beneficial to local communities of the ethnic groups in China.