转Hu-IFN-α基因草鱼雌核发育F_1的遗传配型分析

The Hu-IFN-αgene,which was transducted into downstream promoter of β-actin gene of common carp（Cyprinus carpio）,was recombined by DNA recombination technology.These recombined genes were injected into 1-2 cell fertilized eggs of grass carp（Ctenophatyngodon idellus） by micro-injection technology,we gained transgenetic fish by molecular detection methods.In order to analyse the genetic expression of tran-Hu-IFN-α gene gynogensis F1,which male individualization were gained by raising methyl-testosterone,molecular genetic marker technology was used.In our research,30 random primers were picked out from 48 and were used into RAPD-PCR,the result indicated that 1 169 clear,steady and repeated DNA finger printing bands were achieved.On the basis of gentic distance matrix among tran-Hu-IFN-α gene gynogenesis F1 group,the genetic relationship of gynogenesis F1 were analysed by UPGMA,the results showed the genetic patterns are close between the 3# male gynogenesis F1 and the the 23# female of gynogenesis F1,5# and 27#,2# and 28#,2# and 30#.The data indicated that these group could be served as parent of tran-Hu-IFN-α grass carp（Ctenophatyngodon idellus） pure line.

Web services composition based on global semantics and QoS-aware aspect

A global semantics matching and QoS-awareness service selection are proposed when aimed at a web services composition process.Both QoS-aware matching and global semantic matching are considered during the global matching.When there are demands for global semantic matching and QoS of service composition,a concrete service set which meets the demands is selected for the whole service composition process and an optimal solution is also achieved.A QoS model is built and the corresponding evaluation method is given for the matching of the service composition process.Based on them,a genetic algorithm is proposed to achieve the maximal global semantic matching degree and fulfill the QoS requirements for the whole service composition process.Experimental results and analysis show that the algorithm is feasible and effective for semantics and QoS-aware service matching.

Investigation on Optimization Design of an Equivalent Water Depth Truncated Mooring System Based on INSGA-Ⅱ

At present,equivalent water depth truncated mooring system optimization design is regarded as the priority of hybrid model testing for deep sea platforms,and will replace the full depth system test in the future.Compared with the full depth system,the working depth and span are smaller in the truncated one,and the other characteristics maintain more consistency as well.In this paper,an inner turret moored floating production storage ＆ offloading system（FPSO） which works at a water depth of 320m,was selected to be a research example while the truncated water depth was 80m.Furthermore,an improved non-dominated sorting genetic algorithm（INSGA-II） was selected to optimally calculate the equivalent water depth truncated system,considering the stress condition of the total mooring system in both the horizontal and vertical directions,as well as the static characteristic similarity of the representative single mooring line.The results of numerical calculations indicate that the mathematical model is feasible,and the optimization method is fast and effective.

Tri-level programming model for combined urban traffic signal control and traffic flow guidance

In order to balance the temporal-spatial distribution of urban traffic flow, a model is established for combined urban traffic signal control and traffic flow guidance. With consideration of the wide use of fixed signal control at intersections, traffic assignment under traffic flow guidance, and dynamic characteristics of urban traffic management, a tri-level programming model is presented. To reflect the impact of intersection delay on traffic assignment, the lower level model is set as a modified user equilibrium model. The middle level model, which contains several definitional constraints for different phase modes, is built for the traffic signal control optimization. To solve the problem of tide lane management, the upper level model is built up based on nonlinear 0-1 integer programming. A heuristic iterative optimization algorithm(HIOA) is set up to solve the tri-level programming model. The lower level model is solved by method of successive averages(MSA), the middle level model is solved by non-dominated sorting genetic algorithm II(NSGA II), and the upper level model is solved by genetic algorithm(GA). A case study is raised to show the efficiency and applicability of the proposed modelling and computing method.

Multi-objective optimization of the streamlined head of high-speed trains based on the Kriging model

As the running speed of high-speed trains increases, aerodynamic drag becomes the key factor which limits the further increase of the running speed and energy consumption. Aerodynamic lift of the trailing car also becomes the key force which affects the amenity and safety of the train. In the present paper, a simplified CRH380A high-speed train with three carriages is chosen as the model in order to optimize aerodynamic drag of the total train and aerodynamic lift of the trailing car. A constrained mul- ti-objective optimization design of the aerodynamic head shape of high-speed trains based on adaptive non-dominated sorting genetic algorithm is also developed combining local function three-dimensional parametric approach and central Latin hypercube sampling method with maximin criteria based on the iterative local search algorithm. The results show that local function parametric approach can be well applied to optimal design of complex three-dimensional aerodynamic shape, and the adaptive non-dominated sorting genetic algorithm can be more accurate and efficient to find the Pareto front. After optimization the aerodynamic drag of the simplified train with three carriages is reduced by 3.2%, and the lift coefficient of the trailing car by 8.24%, the volume of the streamlined head by 2.16%; the aerodynamic drag of the real prototype CRH380A is reduced by 2.26%, lift coefficient of the trailing car by 19.67%. The variation of aerodynamic performance between the simplified train and the true train is mainly concentrated in the deformation region of the nose cone and tail cone. The optimization approach proposed in the present paper is simple yet efficient, and sheds lights on the constrained multi-objective engineering optimization design of aerodynamic shape of high-speed trains.