A new bimetallic Cu?complex, [(phen)Cu(μ bdmp)Cu(phen)](ClO4)4·2H2O, where phen and bdmp denote 1,10 phenanthroline and 1,1′ bis(nitrilomethylidyne 4,5 diazafluoren) 4,4′ (μ methylene)bisphenyl respectively w...A new bimetallic Cu?complex, [(phen)Cu(μ bdmp)Cu(phen)](ClO4)4·2H2O, where phen and bdmp denote 1,10 phenanthroline and 1,1′ bis(nitrilomethylidyne 4,5 diazafluoren) 4,4′ (μ methylene)bisphenyl respectively was synthesized and its DNA binding behavior was studied by photophysical methods. It has been found that calf thymus DNA could result in significant decrease both in electronic absorption and fluorescent emission of the complex. The fluorescence quenching by ferrocyanide could be reduced to some extent for the complexin presence of DNA. These facts suggest that this dicopper?complex binds to double helix DNA by partial intercalation.展开更多
0 IntroductionChemistry of manganese complex has become anattractive research field because of the involvement ofmanganese in several biological redox-active systems[1,2],
采用邻二氮菲-Fe2+法测定牦牛乳酪蛋白酶解产物的抗氧化性。通过单因素分析,确定了试剂的用量及反应条件对样品吸光度及羟自由基清除率的影响。优化得到的反应条件为:1 mL 1.5 mmol/L邻二氮菲,2 mLpH7.4磷酸盐缓冲液,1 mL抗氧化剂,充分...采用邻二氮菲-Fe2+法测定牦牛乳酪蛋白酶解产物的抗氧化性。通过单因素分析,确定了试剂的用量及反应条件对样品吸光度及羟自由基清除率的影响。优化得到的反应条件为:1 mL 1.5 mmol/L邻二氮菲,2 mLpH7.4磷酸盐缓冲液,1 mL抗氧化剂,充分摇匀后,加入1 mL 0.5 mmol/L FeSO4溶液,混匀,加1 mL 0.1%H2O2,具塞于38℃反应50 min,于波长536 nm处测定其吸光度。结果表明,改良的邻二氮菲-Fe2+法测定结果重现性好,适于酪蛋白酶解产物抗氧化性的测定。展开更多
文摘A new bimetallic Cu?complex, [(phen)Cu(μ bdmp)Cu(phen)](ClO4)4·2H2O, where phen and bdmp denote 1,10 phenanthroline and 1,1′ bis(nitrilomethylidyne 4,5 diazafluoren) 4,4′ (μ methylene)bisphenyl respectively was synthesized and its DNA binding behavior was studied by photophysical methods. It has been found that calf thymus DNA could result in significant decrease both in electronic absorption and fluorescent emission of the complex. The fluorescence quenching by ferrocyanide could be reduced to some extent for the complexin presence of DNA. These facts suggest that this dicopper?complex binds to double helix DNA by partial intercalation.
文摘0 IntroductionChemistry of manganese complex has become anattractive research field because of the involvement ofmanganese in several biological redox-active systems[1,2],
文摘采用邻二氮菲-Fe2+法测定牦牛乳酪蛋白酶解产物的抗氧化性。通过单因素分析,确定了试剂的用量及反应条件对样品吸光度及羟自由基清除率的影响。优化得到的反应条件为:1 mL 1.5 mmol/L邻二氮菲,2 mLpH7.4磷酸盐缓冲液,1 mL抗氧化剂,充分摇匀后,加入1 mL 0.5 mmol/L FeSO4溶液,混匀,加1 mL 0.1%H2O2,具塞于38℃反应50 min,于波长536 nm处测定其吸光度。结果表明,改良的邻二氮菲-Fe2+法测定结果重现性好,适于酪蛋白酶解产物抗氧化性的测定。
