[Objective] The study was to solve the contradiction between fungus and forest during the production of black fungi, and to obtain relatively high economic benefits and ecological benefits. [Method] Black fungi were c...[Objective] The study was to solve the contradiction between fungus and forest during the production of black fungi, and to obtain relatively high economic benefits and ecological benefits. [Method] Black fungi were cultivated in forest with middle-high altitude of 500-1 600 m. The germination time of fungus stick, germination days of buds, growth days of fruiting body, commodity exterior, marketing effect and biological efficiency of black fungi were investigated. [Result] Compared with traditional cultivation, black fungus products cultivated under forest had the taste and flavor of wild black fungus. Fungus chaff wastage was directly decomposed by the roots in forest land, improving the soil structure of forest land. The need of moisture retention and the frequent access of administrative staff reduced the probability of forest fire occurrence. [Conclusion] Cultivation of black fungus under forest saves more than 70% fixed-asset investment on fungus shed, and the biological efficiency reached 12.2%.展开更多
Dynamic casual modeling of functional magnetic resonance imaging(fMRI) signals is employed to explore critical emotional neurocircuitry under sad stimuli. The intrinsic model of emotional loops is built on the basis...Dynamic casual modeling of functional magnetic resonance imaging(fMRI) signals is employed to explore critical emotional neurocircuitry under sad stimuli. The intrinsic model of emotional loops is built on the basis of Papez's circuit and related prior knowledge, and then three modulatory connection models are established. In these models, stimuli are placed at different points, which represents they affect the neural activities between brain regions, and these activities are modulated in different ways. Then, the optimal model is selected by Bayesian model comparison. From group analysis, patients' intrinsic and modulatory connections from the anterior cingulate cortex (ACC) to the right inferior frontal gyrus (rlFG) are significantly higher than those of the control group. Then the functional connection parameters of the model are selected as classifier features. The classification accuracy rate from the support vector machine(SVM) classifier is 80.73%, which, to some extent, validates the effectiveness of the regional connectivity parameters for depression recognition and provides a new approach for the clinical diagnosis of depression.展开更多
Objective To study the association between the epidermal growth factor(EGF) gene and intelligence in patients with major depression.Methods Intelligence measurement using Wechsler Adult Intelligence Scale(WAIS) was pe...Objective To study the association between the epidermal growth factor(EGF) gene and intelligence in patients with major depression.Methods Intelligence measurement using Wechsler Adult Intelligence Scale(WAIS) was perfor-med on 120 unrelated patients with major depression and 46 control subjects.Blood was collected from all subjects for extraction of genomic DNA.Four single nucleotide polymorphisms(SNPs) in the EGF gene were genotyped using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Results Mean scores of both score lang and score task,two subtests in WAIS,differed significantly between major depression patients and controls(P<0.0001).Quantitative trait analysis showed that the genetype of rs2250724 was closely associated with score lang and score task in major depression patients.The associations were still significant after 10 000 permutations.Conclusions Although preliminary,our results provide evidence for association between the EGF gene and intelligence in patients with major depression.Genetic variation in the EGF gene may increase the susceptibility of major depression.展开更多
Objective: To investigate estrogen receptor β (ERβ) gene Rsa1 polymorphism and concentration of estrogen, FSH and LH in serum in peri-menopausal and menopausal women with depressive disorder. Methods: Seventy-four p...Objective: To investigate estrogen receptor β (ERβ) gene Rsa1 polymorphism and concentration of estrogen, FSH and LH in serum in peri-menopausal and menopausal women with depressive disorder. Methods: Seventy-four peri-menopausal and menopausal women with depressive disorder met ICD-10 and CCMD-3 assessment criteria for depressive disorder were recruited. ERβ gene Rsa1 polymorphism was analyzed with PCR-RFLP. Serum levels of estrogen, FSH and LH were measured by magnetism-ELISA. Results: The respective frequency of ERβ gene Rsa1 polymorphism was no significant difference between women with depressive disorder and the healthy women (χ 2=1.106,P>0.05). The serum level of estrogen was lower in women with depressive disorder than in the healthy women (P<0.05). No difference was found for FSH and LH between two groups. Conclusion: ERβ gene Rsa1 polymorphism may be not associated with depressive disorder in the peri-menopausal and menopausal women. The serum level of estrogen is associated with depressive disorder in the peri-menopausal and menopausal women.展开更多
Selenium protects cells from oxidative damage and reduces lipopolysaccharide (LPS)-induced expression of inflammatory cytokines. Because inflammatory cytokines induce growth depression, we hypothesized that selenium...Selenium protects cells from oxidative damage and reduces lipopolysaccharide (LPS)-induced expression of inflammatory cytokines. Because inflammatory cytokines induce growth depression, we hypothesized that selenium-rich yeast (SeY) would inhibit growth depression caused by LPS. Twenty Meishan weanling pigs [28 (s.d. 2 d), 7.6 (s.d. 0.3 kg) body weight (BW)] were used in a 2×2 factorial design (n=5). The main factors were dietary Se sources [selenium-rich yeast (SeY) vs. sodium selenite (SSe)] with supplement level at 0.3 mg Se/kg, and immunological stress (LPS vs. saline). The experiment lasted for 28 d. On d 14 and d 21, half of pigs in each dietary treatment were intramuscularly injected with either 50 μg/kg BW LPS or an equivalent amount of sterile saline. Blood samples were collected at 3 h post-injection. There were no interactions between dietary Se sources and immunological stress on growth performance. LPS depressed average daily gain (ADG) (d 14-21, P〈0.05; d 21-28, P〈 0.01), and elevated feed to gain ratio (F/G) (d 21-28, P〈0.05), while SeY elevated ADG (d 0-14, P〈0.05; d 21-28 and d 0-28, P〈0.01), and decreased F/G (P〈0.01). Interactions were observed on interleukin-1β(IL-1β) and interleukin-6 (IL-6) from serum samples of d 14 and d 21 (P〈0.01), as well as superoxide dismutase (SOD) and insulin like growth factor (IGF-1) of d 21 (P〈0.05). In conclusion, SeY relieved the growth depression of piglets immunologically stressed by LPS, which maybe resulted from the decreased externalization of inflammatory cytokines and improved antioxidant status.展开更多
Objective:To observe the clinical effects of tuina plus Western medication for functional dyspepsia (FD) due to liver qi stagnation and spleen deficiency.Methods:A total of 72 patients in conformity with the inclu...Objective:To observe the clinical effects of tuina plus Western medication for functional dyspepsia (FD) due to liver qi stagnation and spleen deficiency.Methods:A total of 72 patients in conformity with the inclusion criteria of FD were randomly divided into an observation group and a control group based upon the random number table,36 cases in each group.The control group was treated with mosapride citrate dispersible tablets,and the observation group was treated with the same tablets plus tuina.Before the treatment and 4 weeks after the treatment,the clinical symptoms,quality of life (QOL) and depression severity were observed by the scale,and were followed up two months later after the treatment for assessment of the clinical effects.Results:After the treatment and at the follow-up,the symptom scores of FD and the sores of Hamilton depression rating scale (HAMD) in both groups decreased,and the scores in Chinese version of quality of life questionnaire for functional digestive disorders (Chin-FDDQL) increased,with statistically significant differences in comparison with the same group before the treatment (all P〈0.05).In comparison between the two groups at the same time point after the treatment,the scores of FD symptoms,HAMD and Chin-FDDQL were improved better in the observation group than those in the control group,with statistically significant differences (all P〈0.05).The total effective rates at the follow-up were 91.7% in the observation group and 75.0% in the control group,without statistical difference between the two groups (P〉0.05).The rate of clinical cure and remarkable effect was 66.7% in the observation group,higher than 41.7% in the control group,it is higher in the observation group than that in the control group,with a statistically significant difference between the two groups (P〈0.05).Conclusion:Tuina plus Western medication is precise in the therapeutic effects for FD due to liver qi stagnation and spleen deficiency and can effectively relieve clinical symptoms,elevate the QOL and alleviate depression severity of the patients.Moreover,it's better than the treatment by Western medication alone in the long-term therapeutic effects.展开更多
基金Supported by the Research Fund for the Youth of the Shandong Academy of Agricultural Sciences(2016YQN38)the Independent Innovation Projects for the Universities Institutes of Jinan(201303056)~~
文摘[Objective] The study was to solve the contradiction between fungus and forest during the production of black fungi, and to obtain relatively high economic benefits and ecological benefits. [Method] Black fungi were cultivated in forest with middle-high altitude of 500-1 600 m. The germination time of fungus stick, germination days of buds, growth days of fruiting body, commodity exterior, marketing effect and biological efficiency of black fungi were investigated. [Result] Compared with traditional cultivation, black fungus products cultivated under forest had the taste and flavor of wild black fungus. Fungus chaff wastage was directly decomposed by the roots in forest land, improving the soil structure of forest land. The need of moisture retention and the frequent access of administrative staff reduced the probability of forest fire occurrence. [Conclusion] Cultivation of black fungus under forest saves more than 70% fixed-asset investment on fungus shed, and the biological efficiency reached 12.2%.
基金The National Natural Science Foundation of China(No.30900356,81071135)
文摘Dynamic casual modeling of functional magnetic resonance imaging(fMRI) signals is employed to explore critical emotional neurocircuitry under sad stimuli. The intrinsic model of emotional loops is built on the basis of Papez's circuit and related prior knowledge, and then three modulatory connection models are established. In these models, stimuli are placed at different points, which represents they affect the neural activities between brain regions, and these activities are modulated in different ways. Then, the optimal model is selected by Bayesian model comparison. From group analysis, patients' intrinsic and modulatory connections from the anterior cingulate cortex (ACC) to the right inferior frontal gyrus (rlFG) are significantly higher than those of the control group. Then the functional connection parameters of the model are selected as classifier features. The classification accuracy rate from the support vector machine(SVM) classifier is 80.73%, which, to some extent, validates the effectiveness of the regional connectivity parameters for depression recognition and provides a new approach for the clinical diagnosis of depression.
基金Supported by National High Technology Research and Development Program of China (863 Program) (2006AA02A407)National Basic Research Program of China (973 Program) (2010CB529603)Beijing Natural Science Foundation (7102109)
文摘Objective To study the association between the epidermal growth factor(EGF) gene and intelligence in patients with major depression.Methods Intelligence measurement using Wechsler Adult Intelligence Scale(WAIS) was perfor-med on 120 unrelated patients with major depression and 46 control subjects.Blood was collected from all subjects for extraction of genomic DNA.Four single nucleotide polymorphisms(SNPs) in the EGF gene were genotyped using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Results Mean scores of both score lang and score task,two subtests in WAIS,differed significantly between major depression patients and controls(P<0.0001).Quantitative trait analysis showed that the genetype of rs2250724 was closely associated with score lang and score task in major depression patients.The associations were still significant after 10 000 permutations.Conclusions Although preliminary,our results provide evidence for association between the EGF gene and intelligence in patients with major depression.Genetic variation in the EGF gene may increase the susceptibility of major depression.
文摘Objective: To investigate estrogen receptor β (ERβ) gene Rsa1 polymorphism and concentration of estrogen, FSH and LH in serum in peri-menopausal and menopausal women with depressive disorder. Methods: Seventy-four peri-menopausal and menopausal women with depressive disorder met ICD-10 and CCMD-3 assessment criteria for depressive disorder were recruited. ERβ gene Rsa1 polymorphism was analyzed with PCR-RFLP. Serum levels of estrogen, FSH and LH were measured by magnetism-ELISA. Results: The respective frequency of ERβ gene Rsa1 polymorphism was no significant difference between women with depressive disorder and the healthy women (χ 2=1.106,P>0.05). The serum level of estrogen was lower in women with depressive disorder than in the healthy women (P<0.05). No difference was found for FSH and LH between two groups. Conclusion: ERβ gene Rsa1 polymorphism may be not associated with depressive disorder in the peri-menopausal and menopausal women. The serum level of estrogen is associated with depressive disorder in the peri-menopausal and menopausal women.
文摘Selenium protects cells from oxidative damage and reduces lipopolysaccharide (LPS)-induced expression of inflammatory cytokines. Because inflammatory cytokines induce growth depression, we hypothesized that selenium-rich yeast (SeY) would inhibit growth depression caused by LPS. Twenty Meishan weanling pigs [28 (s.d. 2 d), 7.6 (s.d. 0.3 kg) body weight (BW)] were used in a 2×2 factorial design (n=5). The main factors were dietary Se sources [selenium-rich yeast (SeY) vs. sodium selenite (SSe)] with supplement level at 0.3 mg Se/kg, and immunological stress (LPS vs. saline). The experiment lasted for 28 d. On d 14 and d 21, half of pigs in each dietary treatment were intramuscularly injected with either 50 μg/kg BW LPS or an equivalent amount of sterile saline. Blood samples were collected at 3 h post-injection. There were no interactions between dietary Se sources and immunological stress on growth performance. LPS depressed average daily gain (ADG) (d 14-21, P〈0.05; d 21-28, P〈 0.01), and elevated feed to gain ratio (F/G) (d 21-28, P〈0.05), while SeY elevated ADG (d 0-14, P〈0.05; d 21-28 and d 0-28, P〈0.01), and decreased F/G (P〈0.01). Interactions were observed on interleukin-1β(IL-1β) and interleukin-6 (IL-6) from serum samples of d 14 and d 21 (P〈0.01), as well as superoxide dismutase (SOD) and insulin like growth factor (IGF-1) of d 21 (P〈0.05). In conclusion, SeY relieved the growth depression of piglets immunologically stressed by LPS, which maybe resulted from the decreased externalization of inflammatory cytokines and improved antioxidant status.
文摘Objective:To observe the clinical effects of tuina plus Western medication for functional dyspepsia (FD) due to liver qi stagnation and spleen deficiency.Methods:A total of 72 patients in conformity with the inclusion criteria of FD were randomly divided into an observation group and a control group based upon the random number table,36 cases in each group.The control group was treated with mosapride citrate dispersible tablets,and the observation group was treated with the same tablets plus tuina.Before the treatment and 4 weeks after the treatment,the clinical symptoms,quality of life (QOL) and depression severity were observed by the scale,and were followed up two months later after the treatment for assessment of the clinical effects.Results:After the treatment and at the follow-up,the symptom scores of FD and the sores of Hamilton depression rating scale (HAMD) in both groups decreased,and the scores in Chinese version of quality of life questionnaire for functional digestive disorders (Chin-FDDQL) increased,with statistically significant differences in comparison with the same group before the treatment (all P〈0.05).In comparison between the two groups at the same time point after the treatment,the scores of FD symptoms,HAMD and Chin-FDDQL were improved better in the observation group than those in the control group,with statistically significant differences (all P〈0.05).The total effective rates at the follow-up were 91.7% in the observation group and 75.0% in the control group,without statistical difference between the two groups (P〉0.05).The rate of clinical cure and remarkable effect was 66.7% in the observation group,higher than 41.7% in the control group,it is higher in the observation group than that in the control group,with a statistically significant difference between the two groups (P〈0.05).Conclusion:Tuina plus Western medication is precise in the therapeutic effects for FD due to liver qi stagnation and spleen deficiency and can effectively relieve clinical symptoms,elevate the QOL and alleviate depression severity of the patients.Moreover,it's better than the treatment by Western medication alone in the long-term therapeutic effects.