Zheng Xuan wrote a large number of works in all his life. Now according to a textual research, his works added up to 54 kinds, among which, annotated, were 32 kinds, 15 kinds of Confucian Classics, 10 kinds of Weishu ...Zheng Xuan wrote a large number of works in all his life. Now according to a textual research, his works added up to 54 kinds, among which, annotated, were 32 kinds, 15 kinds of Confucian Classics, 10 kinds of Weishu (books about charms, omens, etc. circulated as appendices to the classics) and 6 kinds of miscellaneous works, besides, 23 kinds of works Zheng Xuan authored by himself. In addition, there were 2 kinds his disciples gathered. As to the sequence of his writing scholarly works, on the whole he first annotated all kinds of Weishu, and then annotated all kinds of Confucian classics. In the 14 years when he was subjected to the interdiction of party politics he wrote more works than any other period and just accomplished Three Books of Rites with annotations and attained perfection in learning.展开更多
[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize a...[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.展开更多
文摘Zheng Xuan wrote a large number of works in all his life. Now according to a textual research, his works added up to 54 kinds, among which, annotated, were 32 kinds, 15 kinds of Confucian Classics, 10 kinds of Weishu (books about charms, omens, etc. circulated as appendices to the classics) and 6 kinds of miscellaneous works, besides, 23 kinds of works Zheng Xuan authored by himself. In addition, there were 2 kinds his disciples gathered. As to the sequence of his writing scholarly works, on the whole he first annotated all kinds of Weishu, and then annotated all kinds of Confucian classics. In the 14 years when he was subjected to the interdiction of party politics he wrote more works than any other period and just accomplished Three Books of Rites with annotations and attained perfection in learning.
基金Supported by Major Projects for Cultivation of New Varieties ofGenetically Modified Organisms (2009ZX08010-011B)
文摘[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.
