An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butan...An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18（2） column（4.6 mm × 250 mm, 5 μm） was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid（0.05%, v/v）. Gradient elution was carried out at the flow rate of 1 m L/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.展开更多
To observe the clinical efficacy of nape cluster acupuncture in treating migraine. Methods： Fifty patients with confirmed diagnosis of migraine were intervened by using nape cluster acupuncture, and were evaluated at...To observe the clinical efficacy of nape cluster acupuncture in treating migraine. Methods： Fifty patients with confirmed diagnosis of migraine were intervened by using nape cluster acupuncture, and were evaluated at the outset and after 2-month treatment by the short-form of McGill pain questionnaire （SP-MPQ） and self-rating depression scale （SDS）. Results： After treatment, the number of positive words, sensory pain rating index （S-PRI）, affective pain rating index （A-PRI）, total pain rating index （T-PRI）, visual analogue scale （VAS）, and present pain intensity （PPI） were significantly changed （P〈0.01）; the SDS score was （56.42＋8.12） points before treatment versus （41.08＋5.73） points after treatment, and the difference was statistically significant （P〈0.01）. The total effective rate was 86.0%; the therapeutic efficacy of the patients with a shorter disease duration was superior to that of the patients with a longer one （P〈0.05）; the efficacy of mild-moderate migraine was superior to that of severe one （P〈0.01）; the total effective rate of patients without depression was higher than that with depression, but without a significant difference in comparing the therapeutic efficacy （P〈0.05）. Conclusion： Nape cluster acupuncture is effective in treating migraine, significantly improving headache and depression.展开更多
基金Foundation for Young Scholars of Shanxi Province,China(Grant No.2011021007-2)
文摘An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18（2） column（4.6 mm × 250 mm, 5 μm） was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid（0.05%, v/v）. Gradient elution was carried out at the flow rate of 1 m L/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.
基金supported by Shanghai Leading Academic Discipline Project~~
文摘To observe the clinical efficacy of nape cluster acupuncture in treating migraine. Methods： Fifty patients with confirmed diagnosis of migraine were intervened by using nape cluster acupuncture, and were evaluated at the outset and after 2-month treatment by the short-form of McGill pain questionnaire （SP-MPQ） and self-rating depression scale （SDS）. Results： After treatment, the number of positive words, sensory pain rating index （S-PRI）, affective pain rating index （A-PRI）, total pain rating index （T-PRI）, visual analogue scale （VAS）, and present pain intensity （PPI） were significantly changed （P〈0.01）; the SDS score was （56.42＋8.12） points before treatment versus （41.08＋5.73） points after treatment, and the difference was statistically significant （P〈0.01）. The total effective rate was 86.0%; the therapeutic efficacy of the patients with a shorter disease duration was superior to that of the patients with a longer one （P〈0.05）; the efficacy of mild-moderate migraine was superior to that of severe one （P〈0.01）; the total effective rate of patients without depression was higher than that with depression, but without a significant difference in comparing the therapeutic efficacy （P〈0.05）. Conclusion： Nape cluster acupuncture is effective in treating migraine, significantly improving headache and depression.
