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水红花子对2/3肝切除PH小鼠肝再生和骨髓细胞增殖的影响 被引量:2
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作者 杨军英 刘锋瑞 《中药药理与临床》 CAS CSCD 北大核心 2010年第5期89-91,共3页
目的:研究水红花子对部分肝切除小鼠骨髓细胞增殖的影响。方法:参照文献方法制备小鼠2/3肝切除模型。给药7d后处死动物,摘取肝脏,计算肝指数;分离小鼠骨髓细胞,流式细胞仪检测DNA含量和细胞周期分布。结果:水红花子3.6、1.8 g/kg使肝切... 目的:研究水红花子对部分肝切除小鼠骨髓细胞增殖的影响。方法:参照文献方法制备小鼠2/3肝切除模型。给药7d后处死动物,摘取肝脏,计算肝指数;分离小鼠骨髓细胞,流式细胞仪检测DNA含量和细胞周期分布。结果:水红花子3.6、1.8 g/kg使肝切除小鼠骨髓细胞DNA含量降低,水红花子3.6、1.8、0.9 g/kg使模型鼠G0/G1期细胞数量增加,而G2/M和S期细胞减少,具有剂量依赖性。PH后小鼠的体重增加缓慢,术后7d时模型鼠的体重和残肝重量明显低于正常对照组。水红花子给药组小鼠的体重和残肝重量明显低于正常对照组,但和模型组比较没有显著性差异。结论:水红花子抑制肝切除小鼠骨髓细胞的增殖。 展开更多
关键词 水红花子 部分肝切除小鼠 骨髓细胞 DNA含量 细胞周期 指数
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Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice 被引量:3
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作者 Norifumi Ohashi Tomohide Hori +5 位作者 Florence Chen Sura Jermanus Christopher B Eckman Akimasa Nakao Shinji Uemoto Justin H Nguyen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第19期2320-2333,共14页
AIM: To investigate the effect of matrix metallopro- teinase-9 (MMP-9) on the remnant liver after massivehepatectomy in the mouse.METHODS: Age-matched, C57BL/6 wild-type (WT), MMP-9(-/-), and tissue inhibitors... AIM: To investigate the effect of matrix metallopro- teinase-9 (MMP-9) on the remnant liver after massivehepatectomy in the mouse.METHODS: Age-matched, C57BL/6 wild-type (WT), MMP-9(-/-), and tissue inhibitors of metalloprotein- ases (TIMP)-1(-/-) mice were used. The mice received 80%-partial hepatectomy (PH). Samples were obtained at 6 h after 80%-PH, and we used histology, immuno- histochemical staining, western blotting analysis and zymography to investigate the effect of PH on MMP-9. The role of MMP-9 after PH was investigated using a monoclonal antibody and MMP inhibitor.RESULTS: We examined the remnant liver 6 h after 80%-PH and found that MMP-9 deficiency attenuated the formation of hemorrhage and necrosis. There were significantly fewer and smaller hemorrhagic and ne- crotic lesions in MMP-9(-/-) remnant livers compared with WT and TIMP-1(-/-) livers (P 〈 0.01), with no dif- ference between WT and TIMP-1(-/-) mice. Serum ala- nine aminotransaminase levels were significantly lower in MMP-9(-/-) mice compared with those in TIMP-I(-/-) mice (WT: 476± 83 IU/L, MMP-9(-/-): 392 ± 30 IU/L, TIMP-I(-/-): 673 ± 73 IU/L, P 〈 0.01). Western blot- ting and gelatin zymography demonstrated a lack of MMP-9 expression and activity in MMP-9(-/-) mice, which was in contrast to WT and TIMP-1(-/-) mice. No change in MMP-2 expression was observed in any of the study groups. Similar to MMP-9(-/-) mice, when WT mice were treated with MMP-9 monoclonal antibody or the synthetic inhibitor GM6001, hemorrhagic and necrotic lesions were significantly smaller and fewer than in control mice (P 〈 0.05). These results suggest that MMP-9 plays an important role in the development of parenchymal hemorrhage and necrosis in the small remnant liver.CONCLUSION: Successful MMP-9 inhibition attenuates the formation of hemorrhage and necrosis and mightbe a potential therapy to ameliorate liver injury after massive hepatectomy. 展开更多
关键词 Matrix metalloproteinase Liver remnant HEPATECTOMY Liver failure NECROSIS
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