The cDNA fragments of interest were amplified using \%Sj\%λ ZipLox library as the templates by PCR and then cloned into a eukaryotic expression vector p\|CMV\|GH; A small number of DNA fragments inserted in the recom...The cDNA fragments of interest were amplified using \%Sj\%λ ZipLox library as the templates by PCR and then cloned into a eukaryotic expression vector p\|CMV\|GH; A small number of DNA fragments inserted in the recombinants was identified by restriction cleavage, EST sequencing and bioinformatical analysis; mice were injected intramuscularly with the expression library(L\|CMV\|SjR) or sublibraries(L\|CMV\|SjR 1,L\|CMV\|SjR 2 and L\|CMV\|SjR 3), immunized mice were challenged with \%Schistosoma japonicum\% cercariae on day 35, the levels of IgG antibodies in sera from the immunized mice were detected by ELISA. The results demonstrated that a partial cDNA expression library of \%S.j,\% with～10\+5 transformants, was constructed, most of the recombinants contained the insert DNA fragments of interest, and these fragments had the features of protein\|coding sequences for \%Schistosome.\% There were no significant differences for the levels of IgG antibodies in sera from all of the immunized groups. Mice immunized with L\|CMV\|SjR, L\|CMV\|SjR 1 and L\|CMV\|SjR 2 developed significant protective effect against \%Sj\% infection compared to control mice injected with the empty plasmid, the rate of worm reduction was about 30%.展开更多
文摘The cDNA fragments of interest were amplified using \%Sj\%λ ZipLox library as the templates by PCR and then cloned into a eukaryotic expression vector p\|CMV\|GH; A small number of DNA fragments inserted in the recombinants was identified by restriction cleavage, EST sequencing and bioinformatical analysis; mice were injected intramuscularly with the expression library(L\|CMV\|SjR) or sublibraries(L\|CMV\|SjR 1,L\|CMV\|SjR 2 and L\|CMV\|SjR 3), immunized mice were challenged with \%Schistosoma japonicum\% cercariae on day 35, the levels of IgG antibodies in sera from the immunized mice were detected by ELISA. The results demonstrated that a partial cDNA expression library of \%S.j,\% with～10\+5 transformants, was constructed, most of the recombinants contained the insert DNA fragments of interest, and these fragments had the features of protein\|coding sequences for \%Schistosome.\% There were no significant differences for the levels of IgG antibodies in sera from all of the immunized groups. Mice immunized with L\|CMV\|SjR, L\|CMV\|SjR 1 and L\|CMV\|SjR 2 developed significant protective effect against \%Sj\% infection compared to control mice injected with the empty plasmid, the rate of worm reduction was about 30%.
