Costaria costata(C.Agardh) Saunders is one of common kelps distributed in many coastal areas worldwide;however,in China,no reports have been made on cultivation of the genus.To investigate potential cultivation of the...Costaria costata(C.Agardh) Saunders is one of common kelps distributed in many coastal areas worldwide;however,in China,no reports have been made on cultivation of the genus.To investigate potential cultivation of the species in the northern part of China,trials on isolation and preservation of the gametophytes were conducted using C.costata from Korea;growth and development of the gametophytes were observed.We showed that at 10±1°C,60 μmol m-2s-1 and 12:12 h(L:D),freshly released zoospores settled down within 1 hour,and then developed into the primary cell during the following 2 days.After a vegetative growth phase lasting 6-8 days,female gametophytes became 3-4 times larger in diameter than that of the primary cell,but still remained at a unicellular stage,while male gametophytes divided into 4-10 cells with only a slight change in size.Fertilization occurred within 10 days after the zoospores were released from the sporangia,and the apical and basal tissues of the juvenile sporophyte divided and differentiated into the blade and stipe.Temperature and irradiance influenced gametophytic vegetative growth and developmental patterns.Generally,low irradiance(15 μmol m-2s-1 and 30 μmol m-2s-1) was unfavorable to the induction of fertility,but it enhanced female gametophyte division.The optimal conditions for vegetative growth were 15°C and 30 μmol m-2s-1.After transplantation of the juvenile seedlings and after eight months cultivation,the harvested mature blade reached 194 cm in length and 32.7 cm in width.Our study proves that it is feasible to implement propagation and large scale cultivation of C.costata in northern China.展开更多
In accordance to National Commodity Policy （2011-2020）, Malaysian government targets at expending the pepper cultivated area from the current 16,331 ha to 20,110 ha by year 2020. Based on a planting density of 2,000...In accordance to National Commodity Policy （2011-2020）, Malaysian government targets at expending the pepper cultivated area from the current 16,331 ha to 20,110 ha by year 2020. Based on a planting density of 2,000 vines/ha, there will be an urgent need of 7.588 million cuttings for the next five years in order to achieve this target. However, shortages of planting materials, low productivity and occurrence of pests and diseases remain as the major challenge of pepper industry nowadays. Therefore, a novel farming innovation, namely W-configuration cultivation method has been developed to ensure high production of planting materials for large-scale pepper cultivation and at the same time to maximize land use in pepper farm. A field evaluation was conducted to compare the cutting production among W-configuration, V-configuration and traditional cultivation method. W-configuration recorded the highest mean number of pepper cuttings produced with 11.97 cuttings per vine in every pruning cycle, or equivalent to 71% of increment as compared to 7.00 cuttings per vine produced from the traditional planting method. Meanwhile, V-configuration produced 8.91 of mean cuttings, which are significantly lower than those produced by W-configuration method and yet comparable to traditional method. Based on return on investment （ROI） analysis, implementation of W-configuration cultivation method was able to achieve 35% of return per cycle of planting within two years for the first cycle and upsurged to approximately 355% for the subsequence cycle. Furthermore, this novel innovation also out-yielded traditional planting method by 75.27% for the first and second year of green berry production. The ROI analysis proved the feasibility of this newly developed cultivation method to create potential new income for pepper smallholders in Malaysia, who traditionally cultivate pepper for its peppercorn production. This study also enlightened the thorough planting procedures of W-configuration cultivation method mainly for pepper cutting production.展开更多
基金Supported by the Shandong Agriculture Seedstocks Projectthe Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-N-247-02)
文摘Costaria costata(C.Agardh) Saunders is one of common kelps distributed in many coastal areas worldwide;however,in China,no reports have been made on cultivation of the genus.To investigate potential cultivation of the species in the northern part of China,trials on isolation and preservation of the gametophytes were conducted using C.costata from Korea;growth and development of the gametophytes were observed.We showed that at 10±1°C,60 μmol m-2s-1 and 12:12 h(L:D),freshly released zoospores settled down within 1 hour,and then developed into the primary cell during the following 2 days.After a vegetative growth phase lasting 6-8 days,female gametophytes became 3-4 times larger in diameter than that of the primary cell,but still remained at a unicellular stage,while male gametophytes divided into 4-10 cells with only a slight change in size.Fertilization occurred within 10 days after the zoospores were released from the sporangia,and the apical and basal tissues of the juvenile sporophyte divided and differentiated into the blade and stipe.Temperature and irradiance influenced gametophytic vegetative growth and developmental patterns.Generally,low irradiance(15 μmol m-2s-1 and 30 μmol m-2s-1) was unfavorable to the induction of fertility,but it enhanced female gametophyte division.The optimal conditions for vegetative growth were 15°C and 30 μmol m-2s-1.After transplantation of the juvenile seedlings and after eight months cultivation,the harvested mature blade reached 194 cm in length and 32.7 cm in width.Our study proves that it is feasible to implement propagation and large scale cultivation of C.costata in northern China.
文摘In accordance to National Commodity Policy （2011-2020）, Malaysian government targets at expending the pepper cultivated area from the current 16,331 ha to 20,110 ha by year 2020. Based on a planting density of 2,000 vines/ha, there will be an urgent need of 7.588 million cuttings for the next five years in order to achieve this target. However, shortages of planting materials, low productivity and occurrence of pests and diseases remain as the major challenge of pepper industry nowadays. Therefore, a novel farming innovation, namely W-configuration cultivation method has been developed to ensure high production of planting materials for large-scale pepper cultivation and at the same time to maximize land use in pepper farm. A field evaluation was conducted to compare the cutting production among W-configuration, V-configuration and traditional cultivation method. W-configuration recorded the highest mean number of pepper cuttings produced with 11.97 cuttings per vine in every pruning cycle, or equivalent to 71% of increment as compared to 7.00 cuttings per vine produced from the traditional planting method. Meanwhile, V-configuration produced 8.91 of mean cuttings, which are significantly lower than those produced by W-configuration method and yet comparable to traditional method. Based on return on investment （ROI） analysis, implementation of W-configuration cultivation method was able to achieve 35% of return per cycle of planting within two years for the first cycle and upsurged to approximately 355% for the subsequence cycle. Furthermore, this novel innovation also out-yielded traditional planting method by 75.27% for the first and second year of green berry production. The ROI analysis proved the feasibility of this newly developed cultivation method to create potential new income for pepper smallholders in Malaysia, who traditionally cultivate pepper for its peppercorn production. This study also enlightened the thorough planting procedures of W-configuration cultivation method mainly for pepper cutting production.
