AIM:To investigate the effect of alcohol on the metabolic syndrome (MS) and fatty liver in Japanese men and women.METHODS:A cross-sectional study was conducted in a medical health checkup program at a general hospital...AIM:To investigate the effect of alcohol on the metabolic syndrome (MS) and fatty liver in Japanese men and women.METHODS:A cross-sectional study was conducted in a medical health checkup program at a general hospital.This study involved 18 571 Japanese men and women,18-88 years of age,with a mean body mass index of 22.6 kg/m 2.A standardized questionnaire was administered.The total amount of alcohol consumed per week was calculated,and categorized into four grades.Fatty liver was examined by ultrasound modified criteria of the revised National Cholesterol Educa-tion Program Adult Treatment Panel Ⅲ and the new International Diabetes Federation.RESULTS:The prevalence of fatty liver decreased in men and women with light to moderate alcohol consumption,whereas the prevalence of MS was not so changed.The prevalence of fatty liver of any grade in men was lower than that in those with no or minimal alcohol consumption.In women with light to moderate alcohol consumption,prevalence of fatty liver was lower than that in women with no or minimal alcohol consumption.By logistic regression analysis,the odds ratio (OR) for MS in women with light alcohol consumption was decreased to < 1.0,but this change was not clear in men.The OR for fatty liver was clearly < 1.0 in men with any level of alcohol consumption and in women with light to moderate consumption.CONCLUSION:Light to moderate alcohol consumption has a favorable effect for fatty liver,but not for MS in Japanese men and women.展开更多
Daily average intake of alcohol during pregnancy has consistently been associated with short term adverse outcomes such as miscarriage, preterm birth and intrauterine growth restriction, a large variety of malformatio...Daily average intake of alcohol during pregnancy has consistently been associated with short term adverse outcomes such as miscarriage, preterm birth and intrauterine growth restriction, a large variety of malformations, as well as long term adverse outcomes such as foetal alcohol syndrome, mental retardation and general impairment of cognitive functions including intelligence, attention, learning abilities as well as social and behavioural functions. Weekly average consumption and alcohol binge drinking （usually defined as ≥ 5 drinks on a single occasion） independently of high daily average intake has not been consistently associated with short and long term adverse outcomes. Health authorities in most countries recommend that pregnant women completely abstain from alcohol. Even so, many health professionals including doctors, midwives and nurses do not provide information to pregnant women in accordance with the offcial recommendations, although a large proportion of women of child bearing age and pregnant women drink alcohol, especially before recognition of pregnancy. The discrepancy between guidelines and the information practice of health personnel is likely to continue to exist because guidelines of abstinence are not clearly evidence-based and not in line with current focus on autonomy and informed choice for patients, and because guidelines do not consider the everyday clinical communication situation.展开更多
Alcohol abuse is a global problem due to the financial burden on society and the healthcare system. While the harmful health effects of chronic alcohol abuse are well established, more recent data suggest that acute a...Alcohol abuse is a global problem due to the financial burden on society and the healthcare system. While the harmful health effects of chronic alcohol abuse are well established, more recent data suggest that acute alcohol consumption also affects human wellbeing. Thus, there is a need for research models in order to fully understand the effect of acute alcohol abuse on different body systems and organs. The present manuscript summarizes the interdisciplinary advantages and disadvantages of currently available human and non-human models of acute alcohol abuse, and identifi es their suitability for biomedical research.展开更多
Oyster extract is an effective bioactivity component. It has abundant nutritional value and antiviral, antitumor and im- mune defense fimctions. The role of oyster extract in treating liver injury has been paid more a...Oyster extract is an effective bioactivity component. It has abundant nutritional value and antiviral, antitumor and im- mune defense fimctions. The role of oyster extract in treating liver injury has been paid more attention. We use Wistar rats to make alcoholic liver disease model through injecting alcohol into rats＇ stomachs. These rats were randomly divided into five groups： model group, control group, low-dose, middle-dose and high-dose experimental group with a dose of 0.12gkg-1, 0.40gkg-1, and 1.20gkg-1 alcoholic. After nine weeks, serum biomarkers （ALT, AST, TG and TCHO）, malondialdehyde （MDA）, glutathione （GSH）, C3a, CSa, IL-17, TNF-a, anti-MAA-HAS IgC~ CD3＋, CD4＋, CDS＋, NK cell activation and zinc content were assessed. The results showed that the serum biomarkers（ALT, AST, TG and TCHO）, MDA content, anti-MAA-HSA IgG, serum C3a, CSa IL-17 and TNF-a levels of oyster extract treatment groups were significantly decreased in comparison with model group. On the contrary, GSH showed ad- verse trend. Serum CD3＋, CD4＋ and NK cell activation were significantly increased in middle-dose group and high-dose group compared with model group, and there was decrease of CD8＋ activity in high-dose group. Plasma Zn level was decreased in model group compared with that in control group. Meanwhile, Mean plasma Zn levels increased dramatically following the dose increase of a given oyster extract.展开更多
Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for...Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for the cryopreservation by vitrification of zygotic embryos of V. vinifera cv. ＂Red Globe＂ and evaluate possible epigenetics changes. The plant vitrification solution 2 （PVS2） was utilized before the utilization of liquid nitrogen （LN）. The effect of this protocol on embryo viability was tested by the triphenyl-tetrazolium chloride solution, as well as by the in vitro development of grape embryos into plantlet. A cDNA expression library of grape zygotic embryos was created to isolate expressed sequence tags of several DNA methyltrasferases. Gene expression of domains rearranged methyltransferase type 1 （DMR1） and DNA （cytosine-5）-methyltransferase 1 （MET1-2） isozymes was analyzed by quantitative reverse transcriptase PCR. The optimal conditions for vitrification were 10 min in 50% PVS2, followed by I0 min in 100% PVS2. Under these conditions, about 30% of plantlet was obtained from embryos after cryopreservation. It was recorded a reduction in the MET1-2 gene expression, which plays a role in the maintenance of DNA methylation. It is possible to cryopreserve viable grape zygotic embryos, although the treatment seems to induce alterations in the normal DNA methylation pattern of the zygotic embryo genome.展开更多
This paper work aims to contribute to active participation of professional authorities into the updating process of education, law and standardization when the preparation of a catastrophic risk plan, in particular fi...This paper work aims to contribute to active participation of professional authorities into the updating process of education, law and standardization when the preparation of a catastrophic risk plan, in particular fire and safety of buildings. Thus will encourage responsibility of Kosovo Government and society to look after the catastrophic events in a structured way, while this approach will enhance effectively and efficiently the level of FP e the buildings, emphasize buildings of cultural heritage of Kosova.展开更多
With the rapid development of the tourist industry in Lijiang, the famous hotels in Lijiang not only bring to the local area the enhancement of the international influence and the levels of the service, but also bring...With the rapid development of the tourist industry in Lijiang, the famous hotels in Lijiang not only bring to the local area the enhancement of the international influence and the levels of the service, but also bring the international experience in the management of the environmental protection. Through the field investigation and interviews, the author compares the characteristics of the environmental protection systems that the branded hotels in Lijiang apply, and summarizes the performances that some of the hotels obtain in the application of the environmental protection systems, and finally puts forward the related suggestions for Lijiang on the construction of the national model city of the environmental protection.展开更多
In this study, a yeast@ZIF-8 core–shell composite material was successfully synthesized under room temperature in aqueous solution. The ZIF-8 shell endowed the inner yeast cells with a considerably extended lifetime ...In this study, a yeast@ZIF-8 core–shell composite material was successfully synthesized under room temperature in aqueous solution. The ZIF-8 shell endowed the inner yeast cells with a considerably extended lifetime without any nutrients at 4°C. Compared with the bare yeast cells, most coated yeast cells were kept alive even when cultured in zymolyase solution for 3h. Furthermore, the encapsulated yeast cells could be reactivated and regrown by dissolving the ZIF-8 shell with competitive coordination interactions.展开更多
基金Supported by A grant from the Gifu Medical AssociationYoung Scientists (B) from Japan Society for the Promotion of Science,No.23790791,in part
文摘AIM:To investigate the effect of alcohol on the metabolic syndrome (MS) and fatty liver in Japanese men and women.METHODS:A cross-sectional study was conducted in a medical health checkup program at a general hospital.This study involved 18 571 Japanese men and women,18-88 years of age,with a mean body mass index of 22.6 kg/m 2.A standardized questionnaire was administered.The total amount of alcohol consumed per week was calculated,and categorized into four grades.Fatty liver was examined by ultrasound modified criteria of the revised National Cholesterol Educa-tion Program Adult Treatment Panel Ⅲ and the new International Diabetes Federation.RESULTS:The prevalence of fatty liver decreased in men and women with light to moderate alcohol consumption,whereas the prevalence of MS was not so changed.The prevalence of fatty liver of any grade in men was lower than that in those with no or minimal alcohol consumption.In women with light to moderate alcohol consumption,prevalence of fatty liver was lower than that in women with no or minimal alcohol consumption.By logistic regression analysis,the odds ratio (OR) for MS in women with light alcohol consumption was decreased to < 1.0,but this change was not clear in men.The OR for fatty liver was clearly < 1.0 in men with any level of alcohol consumption and in women with light to moderate consumption.CONCLUSION:Light to moderate alcohol consumption has a favorable effect for fatty liver,but not for MS in Japanese men and women.
文摘Daily average intake of alcohol during pregnancy has consistently been associated with short term adverse outcomes such as miscarriage, preterm birth and intrauterine growth restriction, a large variety of malformations, as well as long term adverse outcomes such as foetal alcohol syndrome, mental retardation and general impairment of cognitive functions including intelligence, attention, learning abilities as well as social and behavioural functions. Weekly average consumption and alcohol binge drinking （usually defined as ≥ 5 drinks on a single occasion） independently of high daily average intake has not been consistently associated with short and long term adverse outcomes. Health authorities in most countries recommend that pregnant women completely abstain from alcohol. Even so, many health professionals including doctors, midwives and nurses do not provide information to pregnant women in accordance with the offcial recommendations, although a large proportion of women of child bearing age and pregnant women drink alcohol, especially before recognition of pregnancy. The discrepancy between guidelines and the information practice of health personnel is likely to continue to exist because guidelines of abstinence are not clearly evidence-based and not in line with current focus on autonomy and informed choice for patients, and because guidelines do not consider the everyday clinical communication situation.
基金Supported by Grants AA016571 (AD)AA014372 (GS) from NIAAAA (in part)
文摘Alcohol abuse is a global problem due to the financial burden on society and the healthcare system. While the harmful health effects of chronic alcohol abuse are well established, more recent data suggest that acute alcohol consumption also affects human wellbeing. Thus, there is a need for research models in order to fully understand the effect of acute alcohol abuse on different body systems and organs. The present manuscript summarizes the interdisciplinary advantages and disadvantages of currently available human and non-human models of acute alcohol abuse, and identifi es their suitability for biomedical research.
基金financially supported by Grants from Qingdao Technology Office, Qingdao Technology Developing Plan, 10-3-3-3-11-NSH and 03-3-hh-09
文摘Oyster extract is an effective bioactivity component. It has abundant nutritional value and antiviral, antitumor and im- mune defense fimctions. The role of oyster extract in treating liver injury has been paid more attention. We use Wistar rats to make alcoholic liver disease model through injecting alcohol into rats＇ stomachs. These rats were randomly divided into five groups： model group, control group, low-dose, middle-dose and high-dose experimental group with a dose of 0.12gkg-1, 0.40gkg-1, and 1.20gkg-1 alcoholic. After nine weeks, serum biomarkers （ALT, AST, TG and TCHO）, malondialdehyde （MDA）, glutathione （GSH）, C3a, CSa, IL-17, TNF-a, anti-MAA-HAS IgC~ CD3＋, CD4＋, CDS＋, NK cell activation and zinc content were assessed. The results showed that the serum biomarkers（ALT, AST, TG and TCHO）, MDA content, anti-MAA-HSA IgG, serum C3a, CSa IL-17 and TNF-a levels of oyster extract treatment groups were significantly decreased in comparison with model group. On the contrary, GSH showed ad- verse trend. Serum CD3＋, CD4＋ and NK cell activation were significantly increased in middle-dose group and high-dose group compared with model group, and there was decrease of CD8＋ activity in high-dose group. Plasma Zn level was decreased in model group compared with that in control group. Meanwhile, Mean plasma Zn levels increased dramatically following the dose increase of a given oyster extract.
文摘Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for the cryopreservation by vitrification of zygotic embryos of V. vinifera cv. ＂Red Globe＂ and evaluate possible epigenetics changes. The plant vitrification solution 2 （PVS2） was utilized before the utilization of liquid nitrogen （LN）. The effect of this protocol on embryo viability was tested by the triphenyl-tetrazolium chloride solution, as well as by the in vitro development of grape embryos into plantlet. A cDNA expression library of grape zygotic embryos was created to isolate expressed sequence tags of several DNA methyltrasferases. Gene expression of domains rearranged methyltransferase type 1 （DMR1） and DNA （cytosine-5）-methyltransferase 1 （MET1-2） isozymes was analyzed by quantitative reverse transcriptase PCR. The optimal conditions for vitrification were 10 min in 50% PVS2, followed by I0 min in 100% PVS2. Under these conditions, about 30% of plantlet was obtained from embryos after cryopreservation. It was recorded a reduction in the MET1-2 gene expression, which plays a role in the maintenance of DNA methylation. It is possible to cryopreserve viable grape zygotic embryos, although the treatment seems to induce alterations in the normal DNA methylation pattern of the zygotic embryo genome.
文摘This paper work aims to contribute to active participation of professional authorities into the updating process of education, law and standardization when the preparation of a catastrophic risk plan, in particular fire and safety of buildings. Thus will encourage responsibility of Kosovo Government and society to look after the catastrophic events in a structured way, while this approach will enhance effectively and efficiently the level of FP e the buildings, emphasize buildings of cultural heritage of Kosova.
文摘With the rapid development of the tourist industry in Lijiang, the famous hotels in Lijiang not only bring to the local area the enhancement of the international influence and the levels of the service, but also bring the international experience in the management of the environmental protection. Through the field investigation and interviews, the author compares the characteristics of the environmental protection systems that the branded hotels in Lijiang apply, and summarizes the performances that some of the hotels obtain in the application of the environmental protection systems, and finally puts forward the related suggestions for Lijiang on the construction of the national model city of the environmental protection.
基金supported by the National Key Basic Research Program of China (2014CB931801 and 2016YFA0200700)the National Natural Science Foundation of China (2189038, 21721002 and 21475029)+1 种基金Frontier Science Key Project of Chinese Academy of Sciences (QYZDJ-SSW-SLH038)K. C. Wong Education Foundation
文摘In this study, a yeast@ZIF-8 core–shell composite material was successfully synthesized under room temperature in aqueous solution. The ZIF-8 shell endowed the inner yeast cells with a considerably extended lifetime without any nutrients at 4°C. Compared with the bare yeast cells, most coated yeast cells were kept alive even when cultured in zymolyase solution for 3h. Furthermore, the encapsulated yeast cells could be reactivated and regrown by dissolving the ZIF-8 shell with competitive coordination interactions.
